SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Guobin Mao,Wenquan Peng,Songbai Tian,Jiao Zheng,Xinghu Ji,Zhike He
Herein, we developed a simple and sensitive method for the detection of protamine and trypsin by using a ratiometric fluorescent probe based on Rox-DNA functionalized CdZnTeS quantum dots (QDs). The Rox-DNA functionalized CdZnTeS QDs were prepared via one-pot method, which was simple, low-cost, and modification-free. Protamine can cause the aggregation of ratiometric fluorescent probe through electrostatic interaction and quench the fluorescence of CdZnTeS QDs. Then, the protamine could be hydrolyzed to small peptides by trypsin and the fluorescence of QDs was recovered. However, the fluorescence of Rox kept unchanged. The fluorescence intensity ratio was proportional to the concentration of protamine or trypsin. A good linear relationship (25–300 ng/mL for protamine and 2.5–50 ng/mL for trypsin) with low limit of detection (4.73 ng/mL for protamine and 0.87 ng/mL for trypsin) was obtained. Meanwhile, we observed different colors by the naked eye after the addition of different concentrations of protamine and trypsin, and the minimum detectable concentration was as low as 4 μg/mL for protamine and 40 ng/mL for trypsin, respectively. In addition, the ratiometric fluorescent probe exhibited good analytical performance in complex biological matrix. Therefore, the ratiometric fluorescent probe can be proposed as a simple and effective tool for the detection and visualization of dual proteins.
研究领域:
分析化学
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生物化学
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荧光光谱
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Guobin Mao,Qin Cai,Fubing Wang,Changliang Luo,Xinghu Ji,Zhike He
As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate DNA bound to QDs, which were employed as a ratiometric fluorescent probe for the rapid and sensitive detection of H2O2 and glucose. Compared with the traditional multistep construction of ratiometric fluorescent probes, this presented approach is simpler and more effective without chemical modification and complicated separation. The CdZnTeS QDs with green fluorescence is specifically sensitive to H2O2, while the red fluorescence of Rox is invariable. H2O2 is the product from the oxidation of glucose catalyzed by glucose oxidase (GOx). Therefore, a facile method to detect H2O2 and glucose with a detection limit of 0.075 μM for H2O2 and 0.042 μM for glucose was developed. In addition, this proposed probe has been employed for the detection of glucose in human serum with a satisfactory result. Moreover, this probe has been used for visual detection, and the health and diabetics can be distinguished by the naked eye. Meanwhile, this nanoprobe is also generalizable and can be extended to the detection of many other H2O2-mediated analytes.
研究领域:
生物化学
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医学诊断
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
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2023
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IF 11.1
Zhipan Wen,Runqiao Chen,Ge Zhang,Shen Li,Yalei Zhang,Xiaoping Liao,Gang Cheng,Rong Chen
To eliminate environmental risk that induced from organoarsenic feed additive ROX, and enhance special selectivity and recognizability of this micro-polluted aromatic organoarsenicals compound in complex aqueous as well. Novel core-shell magnetic molecularly imprinted polymers (MMIPs) were successfully synthesized through the surface imprinting technology , which circumvented the drawbacks of traditional method such as the deep embedding of the imprinting sites and incomplete elution of the target molecule. Owing to its imprinting effect, the obtained MMIPs displayed excellent behavior and performance for ROX removal compared with magnetic non-imprinted polymers (MNIPs), the binding isotherms of ROX for both MMIPs and MNIPs were better fitted by Langmuir model, and two types of binding sites (specific and non-specific adsorption sites) existed on the surface of MMIPs. The removal efficiency of ROX by MMIPs remained unchanged along with a broad pH range (3.0–10.0), whereas pH seriously affected the ROX removal efficiency of MNIPs. The better uptake of ROX by MMIPs co-existed with competitive substances and two other organic/inorganic arsenic compounds ( p -ASA/As(V)) confirmed the high binding selectivity and special recognizability for its target pollutant. Finally, the application in different spiked real water samples and continuous flow tests further demonstrated MMIPs were the promising candidates and of the potential application future to selectively remove this trace pollutant in different water bodies.
研究领域:
环境科学
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
·
IF 5.401
Dongxia Li,Jin Qin,Guiqin Yan
To solve interference from background fluorescence and scattering light of real biological samples, we developed a room-temperature phosphorescence (RTP) sensor to detect Micrococcal nuclease (MNase). The sensor was based on the phosphorescent resonance energy transfer (PRET) between 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnS quantum dots (QDs) and single-marked oligonucleotide (DNA-ROX). Specifically, the poly-(diallyldimethylammonium chloride) (PDAD)-modified QDs (PDAD-QDs) were prepared as the energy donor, and the DNA-ROX was chosen as the energy receptor. The DNA-ROX could be adsorbed to the surface of the PDAD-QDs through electrostatic interaction, which induced PRET and quenched the RTP of PDAD-QDs. The MNase could efficiently degrade the DNA-ROX into small DNA segments, which were less prone to electrostatic interaction with PDAD-QDs, and thus the PRET efficiency decreased. The RTP intensity of PDAD-QDs was gradually enhanced with the increment of MNase concentration. Under the optimal conditions, the change of RTP intensity was proportional to the logarithm of MNase concentration in the range from 2 × 10 −3 to 8.0 × 10 −2 U mL −1 , with a high correlation coefficient of 0.993 and a detection limit of 6 × 10 −4 U mL −1 . This proposed RTP sensor can avoid interferences from the background fluorescence or scattering light of the matrix that are encountered in spectrofluorometry. Thus, this biosensor can be applied to detect MNase in culture media.
研究领域:
生物化学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Cleaner Production
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2023
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IF 11.1
Zhipan Wen,Shen Li,Ge Zhang,Runqiao Chen,Yalei Zhang,Xiaoping Liao,Gang Cheng,Rong Chen
Magnetic hollow Fe 3 O 4 -based Mg/Al layered double hydroxide (Fe 3 O 4 @SiO 2 @LDH) was successfully synthesized and subsequently the laboratory scale tests were conducted to systematically investigate the sequestration of roxarsone (ROX) and arsenate (As(V)). Owing to its abundant surface metal hydroxyl (M-OH) groups and exchangeable CO 3 2− groups in the interlayer of MgAl-LDH nanosheets , the obtained Fe 3 O 4 @SiO 2 @LDH exhibited remarkable behavior for As(V) and ROX removal. Both As(V) and ROX could be effectively immobilized at a relatively wide pH range (3–8), and the maximum adsorption capacities of As(V) and ROX were 96.47 and 71.67 mg/g, respectively. Meanwhile, the negligible mutual effect demonstrated the Fe 3 O 4 @SiO 2 @LDH can simultaneously remove inorganic and organic arsenic compounds . The competitive cations/anions (except for PO 4 3− ions) and ionic strength were negligible on As(V) and ROX removal, whereas the inhibitory effect stem from humic acid (HA) seemed to be more seriously for As(V) than that of ROX. Adsorption mechanism of As(V)/ROX were attributed to electrostatic attraction and ion/ligand exchange process, whereas hydrogen bonding and π-π interaction may also involve in the ROX removal. Furthermore, the excellent performance in fix-bed column tests and prominent reusability indicated this magnetic Fe 3 O 4 @SiO 2 @LDH was a promising candidate adsorbent and of great potential application in organic/inorganic As-contamination wastewater treatment .
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2016
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IF 5.886
Changhui Liu,Weiju Chen,Zhihe Qing,Jing Zheng,Yue Xiao,Sheng Yang,Lili Wang,Yinhui Li,Ronghua Yang
By virtue of its high sensitivity and rapidity, Fenton reaction has been demonstrated as a powerful tool for in vitro biochemical analysis; however, in vivo applications of Fenton reaction still remain to be exploited. Herein, we report, for the first time, the design, formation and testing of Fenton reaction for in vivo fluorescence imaging of hydrogen peroxide (H2O2). To realize in vivo fluorescence imaging of H2O2via Fenton reaction, a functional nanosphere, Fc@MSN-FDNA/PTAD, is fabricated from mesoporous silica nanoparticle (MSN), a Fenton reagent of ferrocene (Fc), ROX-labeled DNA (FDNA), and a cationic perylene derivative (PTAD). The ferrocene molecules are locked in the pore entrances of MSN, and exterior of MSN is covalently immobilized with FDNA. As a key part, PTAD acts as not only the gatekeeper of MSN but also the efficient quencher of ROX. H2O2 can permeate into the nanosphere and react with ferrocene to product hydroxyl radical (·OH) via Fenton reaction, which cleaves FDNA to detach ROX from PTAD, thus in turn, lights the ROX fluorescence. Under physiological condition, H2O2 can be determined from 5.0 nM to 1.0 μM with a detection limit of 2.4 nM. Because of the rapid kinetics of Fenton reaction and high specificity for H2O2, the proposed method meets the requirement for real applications. The feasibility of Fc@MSN-FDNA/PTAD for in vivo applications is demonstrated for fluorescence imaging of exogenous and endogenous H2O2 in cells and mice. We expect that this work will not only contribute to the H2O2-releated studies but also open up a new way to exploit in vivo Fenton reaction for biochemical research.
研究领域:
生物化学
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纳米技术
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生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2023
·
IF 6.1
Nuo Duan,Yuting Chang,Wenhui Lv,Changxin Li,Chunxia Lu,Zhouping Wang,Shijia Wu
Herein, a SiO 2 @Ag NPs core/shell nanoparticles were synthesized to fabricate a surface-enhanced Raman spectroscopy (SERS) sensor for the simultaneous determination of histamine (HIS) and tyramine (TYR) based on specific aptamer recognition and ratiometric strategy. SiO 2 @Ag NPs with 4-thiosaminophenol (4-ATP) and Nile blue A (NBA) molecules were used as an internal standard (IS) and labeled with aptamers corresponding to HIS and TYR, respectively. Raman reporter molecules ROX and Cy5 labeled complementary DNA (cDNA) were then hybridized with aptamers to form rigid double-stranded DNA. After the HIS and TYR were captured by their aptamers, resulting in the dissociation of cDNA and separated from the SERS substrate. Therefore, the SERS signal intensity at 1503 cm −1 of ROX and 1358 cm −1 of Cy5 tagged on the terminal of cDNA decreased with the concentration of HIS and TYR increasing, while the SERS signal intensity at 1079 cm −1 of 4-APT and 592 cm −1 of NBA on the substrate remain stable. Thus, the concentrations of HIS and TYR can be determined by the I 1503 /I 1079 and I 1358 /I 592 values, respectively. This sensing strategy achieves a lower detection limit of 0.2 ng/mL for HIS and 0.05 ng/mL for TYR, respectively, demonstrating promising applications in sensitive detection of BAs in animal-derived foodstuff.
研究领域:
分析化学
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食品科学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2022
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IF 6.556
Nuo Duan,Tangbin Yao,Changxin Li,Zhouping Wang,Shijia Wu
We designed a SERS-based method to detect β-lactoglobulin (β-Lg), a major allergen of milk, utilizing aptamers to specifically anchor β-Lg. The Au–Ag nanourchins with excellent Raman enhancement effects were prepared and modified with a β-Lg aptamer as the capture substrate. By taking advantage of the hybridization of aptamer and Raman reporter molecule (6-Carboxyl-X-Rhodamine: ROX)-labeled complementary DNA (cDNA), when ROX is close to Au–Ag nanourchins, the SERS signal is enhanced. In the presence of β-Lg, aptamer binding to β-Lg induces the dissociation of ROX-cDNA from Au–Ag nanourchins, thereby decreasing the SERS signal. Under optimal conditions, the present bioassay provides a wide detection range of 10 ng/mL to 1000 ng/mL with a detection limit of 0.07 ng/mL. In addition, the established SERS sensor can selectively and accurately identify β-Lg with recovery rates of 93.25–107.0% in milk samples. It provides great potential as a sensitive and reliable detection method against the growing threat of allergens.
研究领域:
分析化学
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食品科学
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纳米技术
·
过敏原检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
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IF 5.4
Runqiao Chen,Shuheng Yi,Zhipan Wen,Han Feng,Xing Yin,Lingyue Shu,Yalei Zhang
To enhance the special selectivity and recognizability of two kinds of typical phenylarsonic compounds ( p -arsanilic acid ( p -ASA) and roxarsone (ROX)) in complicated water bodies, novel double-template molecularly imprinted polymers (DMIPs) were successfully synthesized via the suspension polymerization method. Subsequently, laboratory scale tests were carried out to systematically investigate the adsorption behavior and performance of p -ASA and ROX by using polymers. The result indicated that DMIPs exhibited super p -ASA/ROX sequestration and immobilization compared with non-imprinted polymers (DNIPs), which was associated with imprinting effect of polymers. The better removal efficiency of p -ASA/ROX by DMIPs were occurred in a broad pH region (3.0–9.0), whereas solution pH severely affected the uptake of DNIPs for organic arsenic compounds. According to the remarkable binding selectivity and special recognizability of DMIPs, common inorganic ions and natural organic matters (NOM) were negligible influence on targeted pollutants removal, and excellent adsorption capacity could still maintain even in the presence of interfering substances that displayed similar chemical structure with templates to a certain extent. Finally, the remarkable reusability and stable performance in continuous flow studies demonstrated the obtained DMIPs were promising candidates for simultaneously and effectively eliminating these multiple organic arsenic pollutants in harsh and complicated aquatic environment.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）