INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Chao Li,Qiang Huang,Xiong Fu,Xiu-Jie Yue,Rui Hai Liu,Li-Jun You
Water-soluble polysaccharides from Prunella vulgaris Linn ( P. vulgaris ) were fractionated using DEAE-Sepharose fast-flow column to obtain several eluents of water (PV-P1), 0.1 M NaCl (PV-P2) and 0.2 M NaCl (PV-P3). Structural analyses showed that PV-P1 had a higher molecular weight and degree of branching as compared to PV-P2 and PV-P3. Tertiary structure analyses indicated that PV-P1, PV-P2 and PV-P3 did not have triple-helical conformation. PV-P2 and PV-P3 showed stronger antioxidant activities than PV-P1, as measured radical scavenging capacities. PV-P1 showed stronger immunomodulatory activities than PV-P2 and PV-P3 in term of stimulation of the production of pro-inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in murine macrophage RAW 264.7 cells. PV-P1, PV-P2 and PV-P3 did not exhibit cytotoxicities against RAW 264.7 at the concentrations tested. These results suggest that P. vulgaris polysaccharides could be explored as potential antioxidant and immunomodulatory agents for the complementary medicine or functional foods.
研究领域:
药理学
·
免疫学
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qiu-Yue Ma,Qian-Da Xu,Nan Chen,Wei-Cai Zeng
A polysaccharide (EOP) from Epiphyllum oxypetalum (DC.) Haw. was isolated and identified, and its immunomodulatory activity was evaluated both in vitro and in vivo . By using multispectral analysis, EOP was determined to be composed of rhamnose , arabinose , galactose , and galacturonic acid at a molar ratio of 26.65:11.48:53.79:6.04, and its molecular weight was 5.77 × 10 6 Da. In addition, backbone structure of EOP was determined to consist of (1 → 4)-linked β-Gal p , (1 → 2)-linked β-Rha p , (1 → 3,4)-linked β-Gal p , (1 → 2,4)-linked β-Rha p and (1 → 4) -linked α-Gal p A, terminating with t-β-Ara p and t-β-Gal p . The in vitro immunomodulatory activity assay on RAW 264.7 cell showed that EOP increased the proliferation of macrophages, enhanced its phagocytic capability, and promoted the production of cytokines including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Furthermore, the in vivo evaluation on zebrafish showed that EOP could reduce the residual content of fluorescent microspheres in zebrafish, which indicated that EOP had the capability to enhance the macrophage phagocytosis . All results suggested that EOP showed a complex structure and exhibited significant immunomodulatory activity both in vitro and in vivo that had the potential to be utilized valuably in food and medicine industries.
研究领域:
植物学
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免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Lihua Geng,Quanbin Zhang,Qiong Li,Qian Zhang,Cong Wang,Ni Song,Wenyu Xin
Silvetia siliquosa , the only species of the family Fucaceae in China, is used as a medicine food homology. Fucoidan from S. siliquosa was extracted by hot water twice thoroughly (13 % of total yield), and a purified fucoidan SSF with a molecular weight of 93 kD was obtained. Chemical composition analysis demonstrated that SSF was primarily composed of sulfate (21.68 wt%) and fucose (84 % of all neutral monosaccharides). IR, methylation analysis, NMR and ESI-MS results indicated SSF had the backbone of mainly (1 → 3)-α-L-fucopyranose and minor (1 → 4)-α-L-fucopyranose, with little 1,3 and 1,4 branched β-D-Xyl p and β-D-Gal p . The in vitro immunomodulatory test on RAW 264.7 cells showed that SSF could up-regulate the expression of immune related factors and proteins in a concentration-dependent manner, but the immunomodulatory effect disappeared from desulfated SSF . This research indicated that highly sulfated fucan possessed immunomodulatory effect and the importance of sulfate groups in the activity of SSF .
研究领域:
药理学
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免疫学
·
海洋生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Minglu Li,Hui Han,Shengmei Song,Shaomin Shuang,Chuan Dong
Fluorescent probes have contributed greatly to our understanding of the biological role of peroxynitrite (ONOO – ). The ONOO – fluorescence probe characterized by the arlyboronate received a moderate opening fluorescence response, and the borate-masked probe significantly increased the sensitivity of ONOO – . Thus, two simple fluorescent probes ( ADB and ANB ) with the recognition receptor of phenyl boronate moiety were constructed for the detection of ONOO – . The change of emission spectrum was affected differently by the electron donating (or withdrawing) of the substituents. ANB was shown to have a low sensitivity and quantum yield towards ONOO – in aqueous solution, whereas ADB with aggregation-induced emission (AIE) process exhibited not only good sensitivity for ONOO – with a detection limit of 75 nM, but also ADB could be used to quantitative detecting ONOO – in response to concentrations of ONOO – within 20 s. Importantly, ADB had good performance for the detection of exogenous ONOO – in the RAW 264.7 cells.
研究领域:
生物化学
·
医学影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 46
·26371-26376
·
IF 2.936
Qiu Li,Qun Li,Zhihui Hao,Xucai Zheng,Wei He
We report a novel water-soluble polysaccharide from Rhizoma panacis japonica, namely, PJ-1 with anti-inflammatory activity. We first obtained a polysaccharide from this herb by eluting with water. After fully characterizing PJ-1 with a series of chromatographic technologies, we evaluated its biological activity by stimulating Raw 264.7 cells to express inflammatory cytokines and found that PJ-1 effectively enhanced the production of anti-inflammatory factors such as IL-10. We further identified its anti-inflammatory effect in a mouse model of sepsis induced by a lipopolysaccharide. The treatment of PJ-1 evidently suppressed the secretion of major inflammatory cytokines and increased the survival rate of mice. Particularly, we verified the STAT3 signal pathway responsible for the anti-inflammatory activity. Thus, PJ-1, which has remarkable anti-inflammatory effect and mechanism of action, may be a promising candidate as an anti-inflammatory agent.
研究领域:
药理学
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免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2019
·Vol. 108
·期 3
·1077-1084
·
IF 2.674
Zhaoliang Guo,Qiannan Zhao,Yuqian Zhang,Bingdong Li,Lijuan Li,Liwei Feng,Manman Wang,Xianguang Meng,Guifu Zuo
An oxidized, porous graphitic carbon nitride nanosheets (CNNs) was successfully obtained via a bottom-up approach. The inner filter effect (IFE) of Fe (III) tetrakis (1-methyl-4-pyridyl) porphyrin pentachlorideporphyrin pentachloride (FeTMPyP) on the CNNs results in fluorescence quenching of the CNNs due to the overlap of FeTMPyP absorbance band and CNNs emission band. It is interesting that the quenched fluorescence could be “turned on” in response to the participation of H 2 O 2 , which caused by decomposition of FeMPyP. In this study, for the first time, a porous fluorescence probe based on CNNs and FeTMPyP was designed and an excellent H 2 O 2 detection performance with a large detection range of 0.1 ~ 100 μM and a detection limit of 0.07 μM was achieved. Furthermore, the proposed method was successfully used for H 2 O 2 detection in RAW 264.7 cells.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Kaiqin Liu,Fuwei Pi,Hongxia Zhang,Jian Ji,Shuang Xia,Fangchao Cui,Jiadi Sun,Xiulan Sun
Inflammation has been shown to play a critical role in the development of many diseases. In this study, we used metabolomics to evaluate the inflammatory effect of lipopolysaccharide (LPS) and the anti-inflammatory effect of glabridin (GB, a polyphenol from Glycurrhiza glabra L. roots) in RAW 264.7 cells. Multivariate statistical analysis showed that in comparison with the LPS group, the metabolic profile of the GB group was more similar to that of the control group. LPS impacted the amino acid, energy, and lipid metabolisms in RAW 264.7 cells, and metabolic pathway analysis showed that GB reversed some of those LPS impacts. Metabolomics analysis provided us with a new perspective to better understand the inflammatory response and the anti-inflammatory effects of GB. Metabolic pathway analysis can be an effective tool to elucidate the mechanism of inflammation and to potentially find new anti-inflammatory agents.
研究领域:
药理学
·
免疫学
·
代谢组学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Jing-yan Guo,Xin-ru Song,Yan-nian Wang,Hui-ming Hua,Shu-meng Ren,Ying-ni Pan,Kun Ren,Yong-fang Sun,Dong-mei Wang,Xiao-qiu Liu
Frankincense (FRA), the oily resin consisting of essential oils, boswellic acids (BAs) and polysaccharides, has been used to improve the blood circulation and relieve pain against carbuncles. According to the theory of traditional Chinese medicine, vinegar processed frankincense (VPF) can increase the effects of promoting blood circulation and relieving pain. Existing studies have carried out much on BAs and essential oils. However, the comparative analysis of polysaccharides from FRA and VPF has not been reported. In this paper, two polysaccharides were isolated and purified from FRA and the other two were from VPF, and their structures and physicochemical properties were analyzed. The immunological and anticoagulatant activities of the four polysaccharides were tested in RAW 264.7 cell and Sprague-Dawley rats, respectively. The polysaccharides purified from VPF showed better immunological and anticoagulatant activities than those in FRA. Therefore, polysaccharides may be one of the active substances for the synergistic effect of VPF.
研究领域:
药理学
·
传统中药学
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生物化学
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免疫学
·
血液学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 24
·4019
·
IF 4.7
Zhou Xu,Yanxia Xiong,Pei Hu,Long Chen,Jianhua Wan,Chenggang Huang,Wenjun Liu
Alpinia officinarumHance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes ofA. officinarum, polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column. The proposed structure was elucidated and the immunoregulatory effects on RAW 264.7 macrophage cells were evaluated. The results showed that AOP-w had a molecular weight of 5.26 kDa, and mainly consisted of galactose and glucose (molar ratio of 0.12:0.88). Its backbone comprised α-(1→4)-Glcp, α-(1→4,6)-Glcpand β-(1→3,4)-Galpresidues, terminated by α-(1→6)-Glcpand T-Glcpresidues. AOP-w was nontoxic to RAW 264.7 cells, but demonstrated promotion in cell proliferation within a 100 μg/mL concentration. The immunostimulatory effects of AOP-w were confirmed by the elevated NO production of AOP-w-treated cells. Moreover, the RNAseq was conducted and the results showed that AOP-w may activate the TNF and NF-κB signaling pathways by binding to Toll-like receptors, thereby affecting the immune modulatory activity of RAW264.7 cells. These results suggest a high potential of AOP-w fromA. officinarumfor immunotherapeutic applications.
研究领域:
药理学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Shasha Cheng,Yuzhen Hou,Xue Wen,Dawei Xu,Xianzhu Luo,Hongshuai Cao
Real-time monitoring of nitric oxide from endogenously cells released is crucial for revealing their physiological activities or pathological processes. However, designing electrochemical sensors for real-time monitoring at the cellular level is challenging due to the simultaneous requirements of sensitivity, transient recording capability, and biocompatibility. Combining the universally self-supported grown highly active materials with stretchable conductive flexible substrates holds promise for achieving sensitive detection of cells and real-time monitoring of mechanically induced biomolecular release. However, existing manufacturing strategies for highly sensitive stretchable flexible electrochemical sensors still face significant challenges due to low material activity, high processing requirements, and the complexity of further designing membrane electrodes. Here, we designed a method of in-situ self-supported growth to grow Co-doped Ni(OH) 2 structures on rigid Ti foil. Leveraging the synergistic effect of Co and Ni, sensitive detection of NO was achieved, enabling real-time monitoring of trace amounts of NO released by Raw 264.7 cells. Based on this material, using catalytic ink method, it was transferred to low-resistance flexible ITO film (LIF), enabling real-time monitoring of mechanically induced NO release from Human Umbilical Vein Endothelial Cells (HUVECs) cells.
研究领域:
生物医学工程
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
LIPIDS
·
2020
·Vol. 55
·期 3
·251-260
·
IF 1.919
Bingnan He,Xia Wang,Xini Jin,Zimeng Xue,Yinhua Ni,Jianbo Zhu,Caiyun Wang,Yuanxiang Jin,Zhengwei Fu
Studies have elucidated that pyrethroids induce adipogenesis. It is also known that macrophages can affect the homeostasis of adipose tissue. However, whether and how the β-cypermethrin (β-CYP)-mediated inhibition of the macrophages affects adipogenesis remain unknown. To explore the effects of β-CYP on adipogenesis through modulating the function of macrophages, 3T3-L1 cells, a preadipocyte cell line, were exposed to culture medium from either RAW 264.7 cells, a macrophage cell line (RM), or β-CYP-treated RAW 264.7 cells (CRM). CRM decreased the inhibitory effects of RM treatment on cell proliferation and adipogenesis, as lipid accumulation, the CEBPA content, and Fasn and Acaca expression in 3T3-L1 cells were higher following CRM treatment than following RM treatment through the higher levels of the demethylated CEBPA promoter in 3T3-L1 cells. However, the medium from β-CYP- and N-acetyl-L-cysteine-cotreated RAW 264.7 cells (CNRM) partially restored the inhibitory effects of RAW 264.7 cells on 3T3-L1 cells that had been reduced by CRM, indicating that β-CYP might reduce the cytotoxicity and inhibitory effects of RAW 264.7 cells on the adipogenesis of 3T3-L1 cells through elevating ROS levels in RAW 264.7 cells. Moreover, exposure to β-CYP downregulated the TNF-α secretion in RAW 264.7 cells. In conclusion, these data demonstrated that β-CYP affected the function of RAW 264.7 cells, alleviating their inhibitory effects on adipogenesis and CEBPA demethylation in 3T3-L1 cells. β-CYP might achieve these effects through downregulating the secretion of TNF-α via elevating ROS levels in RAW 264.7 cells. Our experiments provide a new perspective on the obesogenic effect of pyrethroids.
研究领域:
毒理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Analytical Methods in Chemistry
·
2023
·
IF 2.6
Qianchun Zhang,Haijiang Du,Siqi Xie,Fengling Tian,Xixi Long,Shan Liu,Yun Wu
Hypochlorite (ClO−) has received extensive attention owing to its significant roles in the immune defense and pathogenesis of numerous diseases. However, excessive or misplaced production of ClO− may pose certain diseases. Thus, to determine its biological functions in depth, ClO− should be tested in biosystems. In this study, a facile, one-pot synthesis of nitrogen-fluorine-doped carbon quantum dots (N, F-CDs) was developed using ammonium citrate tribasic, L-alanine, and ammonium fluoride as raw materials under hydrothermal conditions. The prepared N, F-CDs demonstrate not only strong blue fluorescence emission with a high fluorescence quantum yield (26.3%) but also a small particle size of approximately 2.9 nm, as well as excellent water solubility and biocompatibility. Meanwhile, the as-prepared N, F-CDs exhibit good performance in the highly selective and sensitive detection of ClO−. Thus, a wide concentration response range of 0–600 μM with a low limit of detection (0.75 μM) was favorably obtained for the N, F-CDs. Based on the excellent fluorescence stability, excellent water solubility, and low cell toxicity, the practicality and viability of the fluorescent composites were also successfully verified via detecting ClO− in water samples and living RAW 264.7 cells. The proposed probe is expected to provide a new approach for detecting ClO− in other organelles.
研究领域:
分析化学
·
生物医学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）