Journal of Analysis and Testing
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2024
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IF 5.5
Xiao Qi,Zhang Dongyou,Yang Mingli,Liu Shuai,Fang Yi,Huang Shan
A highly sensitive electrochemical aptasensor was developed for the detection of kanamycin, employing a DNA signal amplification strategy that combines RecJf exonuclease-assisted target recycling with a hybridization chain reaction (HCR). The sensing platform is constructed by covalently immobilizing double-stranded DNA (dsDNA) comprised of a kanamycin-specific aptamer and its thiol-modified complementary strand (SH-CDNA) onto a gold electrode. In the presence of kanamycin and RecJf exonuclease, the aptamer selectively binds to kanamycin, dissociating from the dsDNA complex. The RecJf exonuclease then cleaves the aptamer, releasing kanamycin and initiating a cycle of repetitive binding and release. The residual SH-CDNA on the electrode triggers an HCR between two types of ferrocene-labeled hairpin DNA, forming an elongated stable dsDNA nanostructure. This results in an amplified electrochemical signal proportional to the logarithm of kanamycin concentration over a range of 0.01–10 nmol/L, with a remarkable detection limit of 1.8 pmol/L. The aptasensor’s performance was validated by analyzing spiked kanamycin in pharmaceutical eye drops and milk samples, yielding recovery rates between 95.6% and 104.8% and relative standard deviations from 1.4% to 4.2%. With its exceptional selectivity and sensitivity, this aptasensor offers a compelling alternative to traditional HPLC for rapid on-site detection of kanamycin. Capitalizing on the specificity of aptamers, the sensor design presented herein serves as a valuable blueprint for engineering detectors of other molecules, with significant implications for analytical chemistry and food safety monitoring.
研究领域:
分析化学
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食品安全
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生物传感
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DOI
高影响因子
Food Bioscience
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2025
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IF 5.9
Tao Lu,Zhiguang Suo,Min Wei,Huali Jin,Yong Liu,Baoshan He,Renyong Zhao
β-lactoglobulin (β-LG) is a major allergen in milk what can provoke intense allergic reactions. Therefore, an accurate and efficient method is required for sensitive detection of β-LG. A label-free electrochemical aptasensor utilizing Au/Ag@ZIF-8 and dual amplification strategy has been designed for the detection of β-LG. Au/Ag@ZIF-8 nanocomposite is used as the electrode modification material, which has abundant relative surface area and exceptional conductivity behavior, and it can also serve to immobilize DNA strands. By combining RecJf exonuclease and hybridization chain reaction (HCR) dual amplification strategy, an apparent enhancement of electrochemical signal is obtained. β-LG undergoes target cycling in the effect of RecJf exonuclease, which dislodges complementary strand cDNA and further triggers HCR to provide a large number of sites for methylene blue embedding. The detection range of this aptasensor is 0.01–1000 ng/mL in the optimal detection conditions, with a limit of detection (LOD) of 5.37 pg/mL. The constructed electrochemical aptasensor possesses the superiorities of good specificity, preeminent accuracy and satisfied stability in the detection of β-LG. Moreover, it also demonstrates satisfactory detection performance in the detection of real samples, which indicates that the electrochemical aptasensor has a broad application prospect in protecting food safety and health.
研究领域:
食品安全检测
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生物传感技术
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分析化学
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DOI
高影响因子
TALANTA
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2016
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IF 4.035
Meng Chen,Ning Gan,You Zhou,Tianhua Li,Qing Xu,Yuting Cao,Yinji Chen
An ultrasensitive electrochemical aptasensor for simultaneous detection of oxytetracycline (OTC) and kanamycin (KAN) has been developed based on metal ions doped metal organic frame materials (MOFs) as signal tracers and RecJ f exonuclease-catalyzed targets recycling amplification. The aptasensor consists of capture beads (the anti-single-stranded DNA Antibody, as anti-ssDNA Ab, labeled on Dynabeads) and nanoscale MOF (NMOF) based signal tracers (simplified as Apts-MNM, the NMOF labeled with metal ions and the aptamers). Particularly, the MOF (UiO-66-NH 2 ), with large internal surface areas, ultrahigh porosity and abundant amine groups in the pores, was employed as substrates to carry plenty of metal ions (Pb 2+ or Cd 2+ ) and label aptamers of OTC or KAN. Thus, the aptasensor is formed by the specific recognition between anti-ssDNA Ab and aptamers. In the presence of targets (OTC and KAN), aptamers prefer to form targets-Apts-MNM complexes in lieu of anti-ssDNA Ab-aptamer complexes, which results in the dissociation of Apts-MNM from capture beads. With the employment of RecJ f exonuclease, targets-Apts-MNM in supernatant was digested into mononucleotides and liberated the target, which can further participate in the next reaction cycling to produce more signal tracers. After magnetic separation, the enhanced square wave voltammetry (SWV) signals were produced from signal tracers. The aptasensor exhibited a linear correlation in the range from 0.5 pM to 50 nM, with detection limits of 0.18 pM and 0.15 pM (S/N=3) toward OTC and KAN respectively. This strategy provides specificity and sensitive approach for multiplex antibiotics detection and has promising applications in food analysis.
研究领域:
分析化学
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环境科学
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食品安全
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生物传感器
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
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2022
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IF 5.76
Cuiying Lin,Qingqing Huang,Xiang Hong,Shichai Hong,Xiaolong Shu,Enci Wang,Lixin Wang,Weiguo Fu,Zhenyu Lin
Vascular endothelial growth factor (VEGF 165 ) is a signal protein that plays a central role in the regulation of angiogenesis and can stimulate angiogenesis. The development of highly sensitive and selective detection method for VEGF 165 is very important for disease diagnosis and follow-up treatment monitoring. In this study, an electrochemiluminescence (ECL) aptasensor for VEGF 165 has been developed based on quench of H 2 O 2 toward Ru(bpy) 3 2+ /TPrA ECL system and RecJ f exonuclease induced target recovery and hybridization chain reaction (HCR) as amplification strategy. The presence of VEGF 165 makes a large number of glucose oxidase (GOD) fixed on the electrode surface through the double signal amplification strategies. The present of GOD cause the production of a large amount of H 2 O 2 near the electrode surface under excess amount of glucose, resulting in the inhibition of the ECL signal of Ru(bpy) 3 2+ /Au nanoparticles (Ru(bpy) 3 2+ /AuNPs) film fixed on the electrode surface. The ECL response of the designed biosensor has a good linear relationship with the logarithm of the concentration of VEGF 165 in the range of 0.5 pg/mL to 500 ng/mL with a detection limit of 0.2 fg/mL. The VEGF 165 in serum samples has been detected by the proposed aptasensor with satisfactory results.
研究领域:
生物传感器
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分析化学
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生物医学工程
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分子生物学
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临床诊断
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DOI
PubMed
高影响因子
Food Chemistry-X
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2024
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IF 6.5
Hua Zheng,Linlin Feng,Zheng Huang,Ziwei Zou,Xiaolong Ma,Ziping Pan,Jinfeng Li,Jinxia Wu,Mei Li,Zhiheng Su
Aflatoxin B1 (AFB1) is a potent toxin in food, necessitating rapid, instant, and sensitive detection. We have engineered an electrochemical sensor to monitor AFB1 using a system composed of Fe 3 O 4 -NH 4 /AuNPs/apt-S1. The aptamer specifically recognizes AFB1, while ‘S1’ is functionalized with methylene blue to enhance the current. The RecJf exonuclease promotes the formation of the electrochemical strategy. The Fe 3 O 4 component, with its magnet properties, enables a rapid separation of solids and liquids without the need for instrumentation. The sensor exhibits a linear range for AFB1 ranging from 1 ng to 10 μg. The regression equation is I(nA) = 446.8 × logc+2085 (where I and c represent the peak current and AFB1 concentration, respectively). The correlation coefficient is 0.9508, and the detection limit is 3.447 nM. The relative standard deviation of AFB1 in peanut oil ranges from 4.80% to 6.80%. These results demonstrate that the sensor has high sensitivity, stability, repeatability, and specificity for AFB1 detection.
研究领域:
食品安全
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分析化学
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电化学
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毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）