Xiaoqian Xie,Mingfei Pan,Liping Hong,Kaixin Liu,Jingying Yang,Shan Wang,Shuo Wang
In this study, the rhodamine 6G hydrazide (R6GH) complex was synthesized to develop an “off–on” output platform for fluorescence and visual dual-mode analysis of lead(II) (Pb2+). The prepared R6GH complex using the heat to reflux reaction of rhodamine 6G (R6G) and hydrazine hydrate was characterized through FT-IR, MS, 1H NMR, and 13C NMR and demonstrated to have good fluorescence stability and reversibility. The microenvironment for Pb2+ detection has been optimized in detail. Under the optimal conditions, the “off–on” R6GH-based fluorescence output platform showed a good response to Pb2+ in the concentration range of 0.05–6.0 μM (R2 = 0.9851) with a limit of detection (LOD) of 0.02 μM. Furthermore, at three spiked Pb2+ levels in the selected agricultural (tap water, soil) and food (fish, shrimp) samples, the developed R6GH-based fluorescence assays obtained a significant recovery range of 84.0–102.0% (RSD < 5.0%, n = 3), which had a good correlation with the results from ICP-MS (R2 = 0.9915). The developed R6GH immobilized paper-based array sensor can reach the lower LOD (2.5 μM) for Pb2+ through the naked eye. By combining with LAB analysis, a good linear response was obtained in the Pb2+ concentration range of 1.0–50.0 μM. These results indicated that the developed R6GH probe had great application potential in accurate detection of fluorescence and rapid visual and semiquantitative screening for Pb2+.
Ziwen Zhang,Ran Han,Sixuan Chen,Feilin Zheng,Xinmiao Ma,Mingfei Pan,Shuo Wang
In this study, a rapid fluorescent and colorimetric dual-mode detection strategy for Hg2+in seafoods was developed based on the cyclic binding of the organic fluorescent dye rhodamine 6G hydrazide (R6GH) to Hg2+. The luminescence properties of the fluorescent R6GH probe in different systems were investigated in detail. Based on the UV and fluorescence spectra, it was determined that the R6GH has good fluorescence intensity in acetonitrile and good selective recognition of Hg2+. Under optimal conditions, the R6GH fluorescent probe showed a good linear response to Hg2+(R2= 0.9888) in the range of 0–5 μM with a low detection limit of 2.5 × 10−2μM (S/N = 3). A paper-based sensing strategy based on fluorescence and colorimetric analysis was developed for the visualization and semiquantitative analysis of Hg2+in seafoods. The LAB values of the paper-based sensor impregnated with the R6GH probe solution showed good linearity (R2= 0.9875) with Hg2+concentration in the range of 0–50 μM, which means that the sensing paper can be combined with smart devices to provide reliable and efficient Hg2+detection.Keywords:Hg2+;R6GH;dual mode;fluorescence;visualization
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