INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Yao Xu,Huiyan Jiang,Congyong Sun,Michael Adu-Frimpong,Wenwen Deng,Jiangnan Yu,Ximing Xu
In this study, two polysaccharide fractions (RRP1: Mw = 5.5 kDa, and RRP2: Mw = 425.7 kDa) were isolated from Rhodiola rosea to investigate their antioxidation and hepatoprotective effects. Physicochemical analysis showed that RRP1 was composed of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose with a relative molar ratio of 0.69:0.11:0.15:1:0.51:7.5 and RRP2 was consisted of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose (relative molar ratio = 0.15:0.19:1.01:0.18:0.47:1). Periodate oxidation and Smith degradation analysis revealed that, in RRP1, part of the arabinose and glucose residues were 1 → 3,6/1 → 3/1 → 2,3/1 → 3,4/1 → 2,4/1 → 2,3,4-linked, and the mannose, rhamnose and galactose residues were 1 → 2,6/1 → 6/1 → 2/1→/1 → 4,6/1 → 4-linked. In RRP2, the rhamnose, glucose and galactose residues were linked by 1 → 3,6/1 → 3/1 → 2,3/1 → 3,4/1 → 2,4/1 → 2,3,4 linkages, and the arabinose and mannose residues were 1 → 2/1 → 6/1 → 4-linked. The methylation analysis confirmed the structure information of the two fractions. Importantly, fraction RRP1 demonstrated stronger antioxidative activities than RRP2 by scavenging DPPH, hydroxyl and superoxide anion radicals in vitro. Correspondently, RRP1 showed more significant effects than RRP2 on decreasing the levels of ALT, AST and MDA, and increasing the GSH, SOD and CAT levels in the CCl 4 -treated mice. These data demonstrated that the polysaccharide RRP1 could be developed as a promising candidate for preventing and treating liver damage induced by toxic chemicals.
研究领域:
药理学
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生物化学
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DOI
PubMed
高影响因子
ACS Omega
·
2021
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IF 3.512
Daihua Hu,Xu Yang,Wang Chen,Zili Feng,Chingyuan Hu,Fei Yan,Xiaohua Chen,Dong Qu,Zhiyuan Chen
The silver nanoparticles (AgNPs) using the rhizome extract of Rhodiola rosea have been reported. However, their antioxidant activity and whether the biogenic AgNPs could be used to catalyze the reduction of hazardous dye or used as fluorescence enhancers are unknown. This study focused on the facile green synthesis of silver nanoparticles using the rhizome aqueous extract of R. rosea (G-AgNPs). We then studied their antioxidant activity and catalytic degradation of hazardous dye Direct Orange 26 (DO26) and Direct Blue 15 (DB15). Their effects on fluorescein’s fluorescent properties were also evaluated. The chemical AgNPs (C-AgNPs) were synthesized by reducing solid sodium borohydride (NaBH4), and its above activities were compared with those of G-AgNPs. The formation of G-AgNPs was confirmed by the appearance of brownish-gray color and the surface plasmon resonance (SPR) peak at 437 nm. The biogenic AgNPs were approximately 10 nm in size with a regular spherical shape identified from transmission electron microscopy (TEM) analysis. G-AgNPs exhibited significantly improved 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity than butylated hydroxytoluene (BHT) and C-AgNPs (p < 0.05). The biogenic G-AgNPs were also found to function as an effective green catalyst in reducing DO26 and DB15 by NaBH4, which is superior to C-AgNPs. Furthermore, G-AgNPs showed better fluorescence enhancement activity than C-AgNPs, and the concentration required was lower. When the concentration of the G-AgNP solution was 64 nmol/L, the fluorescence intensity reached the maximum of 5460, with the fluorescence enhancement efficiency of 3.39, and the fluorescence activity was stable within 48 h. This study shows the efficacy of biogenic AgNPs in catalyzing the reduction of hazardous dye DO26 and DB15. Biogenic AgNPs could also be used as fluorescence enhancers in low concentrations.
研究领域:
纳米生物学
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环境科学
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药理学
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DOI
PubMed
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
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2026
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IF 4.7
Wenting Gao,Bin Mu,Fangfang Yang,Yalong Li,Yu Tang,Zhihe Luo,Aiqin Wang
The potential of chitosan (CS) films for the preservation has been explored, and it is recognized as one of the promising approaches to enhancing the comprehensive capability of CS films for food control after incorporation of functional nanofillers. Herein, Rhodiola rosea extract/ZnO/palygorskite (RRE/ZnO/Pal) nanocomposites were facilely prepared and incorporated into CS matrix to obtain RRE/ZnO/Pal/CS (RZPC) composite films, in which ZnO/Pal nanocomposites were synthesized by a green phyto-mediated method followed by loading of R. rosea extract via a semi-dry grinding process to form organic/inorganic bioactive nanocomposites. The composite films exhibited significantly enhanced antioxidant, antibacterial, mechanical and UV-shielding properties compared with the pure CS film, as well as the maximum scavenging activities on hydroxyl radicals and 2,2-diphenyl-1-picrylhydrazine radicals of composite films were 92.85% ± 1.59% and 56.89% ± 0.56%, respectively. The antibacterial ratios of composite films containing 6.25% of RRE/ZnO/Pal nanocomposites were 94.92% ± 2.67% and 95.48% ± 2.08% for E. coli and S. aureus , respectively. In addition, the RZPC films effectively delayed browning and deterioration of bananas. This study provides a green method for construction of bioactive nanocomposites and new strategy to design multifunctional CS films for food preservation based on the synergistic effect of organic and inorganic components.
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高影响因子
PLANT CELL TISSUE AND ORGAN CULTURE
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2026
·Vol. 165
·期 1
·12
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IF 2.8
Erst Anna A.,Kotsupiy Olga V.,Tomoshevich Maria A.,Banaev Evgeny V.
For the first time, a mixoploid plants and callus cultures of Rhodiola rosea L. were established. Flow cytometry analysis revealed that seed treatment with 1000 µM colchicine for 24 hours induced mixoploidy in 30% of the samples, while the remaining 70% remained diploid. The combination of 5 µM thidiazuron and 5 µM α-naphthaleneacetic acid resulted in a 100% callus induction rate from cotyledonary leaves and supported callus growth up to 50 g/L dry weight. The phenylpropanoids identified in the rhizomes of in vitro plants (control, 2n) were rosavin, rosin, unidentified phenylpropanoids (PP1, PP2, and PP3), and cinnamyl alcohol. In the mixoploid calluses, the same phenylpropanoids were detected, with the exception of PP1. In the diploid calluses, no phenylpropanoids were detectable within the sensitivity limits of the analytical method used. ANOVA confirmed the significant influence of both UV exposure and cinnamyl alcohol concentration on phenylpropanoid accumulation in mixoploid calluses, with levels reaching up to 484 mg/100 g dry weight. Rosin was identified as the major phenylpropanoid in mixoploid calluses under standard conditions. However, after 72 hours of UV irradiation and cinnamyl alcohol supplementation, PP3 became the dominant compound. This study demonstrates that combining polyploidization, biotransformation, and elicitation is a promising alternative strategy for enhancing metabolite production in Rhodiola rosea cell cultures. Moreover, the targeted application of these stress factors can serve as a tool for ‘metabolic engineering’ in its callus culture. This approach may facilitate the sequential scale-up of metabolite biosynthesis in bioreactors to meet industrial demands.
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DOI
高影响因子
World Journal of Traditional Chinese Medicine
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2026
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IF 3.2
Yang Shu-Xian,Liu Shu-Sen,Zhi Chen-Xiao,Wei Biao,Cheng Jin-Jun,Zhao Zhi-Gang,Bai Ming-Hua,Wang Bin,Zhu Bin
Objective: An integrated approach combining network pharmacology and experimental verification was used to elucidate the effects and mechanisms of the Scutellaria baicalensis (Huangqin [HQ]) and Rhodiola rosea (Hongjingtian [HJT]) herbal pair in treating ischemic stroke (IS). Materials and Methods: A compound–disease network for the HQ-HJT herbal pair and IS was constructed for the network pharmacology analysis. In addition, the efficacy and targets of the HQ-HJT herbal pair were verified using a classical in vitro IS model and molecular docking methods. Results: A total of 82 overlapping targets were identified between the HQ-HJT herbal pair and IS. Kaempferol was the most active compound in the compound–disease network. The protein-protein interaction network analysis showed that Interleukin-6, tumor necrosis factor-α (TNF-α), and HIF-1α were core targets. Kyoto Encyclopedia of Genes and Genomes analysis identified that the HQ-HJT herbal pair exerts therapeutic effects on IS through the TNF signaling and other related pathways. Our experimental analyses confirmed that the key components (kaempferol, salidroside, and baicalein) had neuroprotective effects in the oxygen-glucose deprivation/reoxygenation injury model. Notably, kaempferol suppressed inflammatory responses and showed strong binding affinity to six key targets within the HIF-1α/NLRP3/Caspase-1 signaling axis. Conclusions: HQ-HJT may exert an anti-IS effect by inhibiting the inflammatory cascade. Kaempferol may serve as a key bioactive compound in the HQ-HJT pair.
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高影响因子
Journal of Functional Foods
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2020
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IF 3.701
Shan Shan Qi,Meng Li Shao,Sun Ze,Hong Xing Zheng
Salidroside from Rhodiola rosea L. has hypoglycemic effects, and has anti-osteoporosis effect in diabetic rats. Whether it has beneficial effects on diabetic nephropathy (DN) has not been fully reported. Here, a DN rat model was established by streptozotocin (STZ). Salidroside (10, and 20 mg kg −1 d −1 ) were orally giving for eight weeks. The purity of salidroside was 99%. The results showed that eight weeks salidroside supplementation dose-dependently decreased blood glucose, serum insulin, serum creatinine, blood urea nitrogen (BUN), as well as urinary albumin of DN rats, repaired the pathological changes of kidney tissue, the renal sclerosis and fibrosis in the kidney of DN rats were improved, and the activities of oxidative defense system associated enzyme such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were also improved. Serum inflammatory cytokines (including tumor necrosis factor-α, monocyte chemoattractant protein-1, interleukin-1β, and interleukin-6) were decreased significantly in DN rats after eight weeks salidroside supplementation. Immunohistochemical results showed that fibrosis related proteins such as transforming growth factor-β1 (TGF-β1), phospho-Smad2 (p-Smad2), and phospho-Smad3 (p-Smad3) expression levels were decreased significantly in the kidney of salidroside treated group. Our results indicate that daily salidroside consumption could alleviate DN in rats, the main mechanisms are anti-inflammatory, anti-oxidative stress, and inhibition of the TGF-β1/Smad2/3 pathway. This study suggested that salidroside could be used as a nutrient against DN, as well as diabetes.
研究领域:
药理学
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糖尿病学
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肾病学
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高影响因子
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