CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Xuejing Jia,Chao Zhang,Jianfeng Qiu,Lili Wang,Jiaolin Bao,Kai Wang,Yulin Zhang,Meiwan Chen,Jianbo Wan,Huanxing Su,Jianping Han,Chengwei He
Three novel acidic polysaccharides termed PRM1, PRM3 and PRM5 were purified from Rhynchosia minima root using DEAE-52 cellulose and sephadex G-150 column chromatography. Their structures were characterized by ultraviolet (UV) and Fourier transform infrared (FTIR) spectrometry, gel permeation chromatography (GPC), gas chromatography–mass spectrometry (GC–MS), and differential scanning colorimeter (DSC) analysis. The uronic acid contents of PRM1, PRM3 and PRM5 were 30.7%, 12.7% and 47.7%, respectively. PRM1 (143.2 kDa), PRM3 (105.3 kDa) and PRM5 (162.1 kDa) were heteropolysaccharides because they were composed of arabinose, mannose, glucose and galactose. Their enthalpy values were 201.0, 111.0 and 206.8 J/g, respectively. PRM3 and PRM1 exhibited strong in vitro anticancer activity against lung cancer A549 and liver cancer HepG2 cells in a dose-dependent manner. These findings suggested that PRM1 and PRM3 could be potentially developed as natural anticancer agents.
研究领域:
植物化学
·
药理学
·
肿瘤学
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DOI
高影响因子
Journal of Functional Foods
·
2016
·
IF 3.973
Xuejing Jia,Jie Hu,Muxue He,Qingwen Zhang,Peng Li,Jianbo Wan,Chengwei He
Rhynchosia minima root, a renowned traditional Chinese medicinal herb, has been shown to exhibit multiple therapeutic activities. In this study, the α-glucosidase inhibitory activity of R. minima root polysaccharide fractions (PRM1, PRM3 and PRM5) is evaluated. PRM3 displays an excellent inhibitory activity with an IC 50 value of 8.85 mg/mL, which is similar to that of acarbose, a positive control compound that has been applied for the control of postprandial hyperglycemia. PRM3 is a homogeneous fraction with an average molecular weight of 97.3 kDa. The results of methylation analysis, periodate oxidation and nuclear magnetic resonance (NMR) spectra reveal that the repeating units of PRM3 are mainly composed of (1 → 3,4)-α-Ara p , (1 → 4,6)-β-Gal p , (1 → 2,4)-β-Rha p , (1 → 3,4)-β-Gal p A, T-α-Ara f and T-α-Gal p residues. Moreover, triple helix stereo-configuration has been proven to exist in PRM3. In summary, the strong α-glucosidase inhibitory activity and unique structural feature of PRM3 suggest that it could have a potential application in functional foods.
研究领域:
中药学
·
生物化学
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产品关联
DOI
高影响因子
MOLECULES
·
2015
·Vol. 20
·期 11
·20901-20911
·
IF 2.416
Xuejing Jia,Chao Zhang,Jie Hu,Muxue He,Jiaolin Bao,Kai Wang,Peng Li,Meiwan Chen,Jianbo Wan,Huanxing Su,Qingwen Zhang,Chengwei He
Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides fromRhynchosia minimaroot (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions were also measured. The results showed that optimal extraction parameters were as follows: ultrasound exposure time, 21 min; ratio of water to material, 46 mL/g; ultrasound extraction temperature, 63 °C. Under these conditions, the maximum yield of PRM was 16.95% ± 0.07%. Furthermore, the main monosaccharides of purified fractions were Ara and Gal. PRM3 and PRM5 exhibited remarkable DPPH radical scavenging activities and reducing powerin vitro. PRM3 showed strong inhibitory activities on the growth of MCF-7 cellsin vitro. The above results indicate that polysaccharides fromR. minimaroot have the potential to be developed as natural antioxidants and anticancer ingredients for the food and pharmaceutical industries.Keywords:Rhynchosia minimaroot;polysaccharide;ultrasound-assisted extraction;antioxidant activity;anticancer activity
✓ 已验证
DOI
PubMed
高影响因子
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