Extraction, purification, chemical characterization, and in vitro hypoglycemic activity of polysaccharides derived from Rosa laevigata Michx.
This study aimed to optimize the extraction process of polysaccharides from Rosa laevigata Michx. (RLMP) using an ultrasound-microwave-assisted method and investigate its in vitro hypoglycemic activity. Key factors affecting RLMP yield were identified using a Plackett–Burman design, followed by a Box–Behnken design and response–surface methodology, to determine the optimal extraction conditions. RLMP was purified using DEAE-52 cellulose, yielding two homogeneous fractions: RLMP-1 and RLMP-2. Monosaccharide composition was analyzed by gas chromatography, and structural characterization of RLMP, RLMP-1, and RLMP-2 was performed using FT-IR, SEM, and TEM. Methylation analysis and NMR were used to elucidate the sugar-chain structure of RLMP-1. In vitro hypoglycemic activity analysis showed that RLMP improved the glucose consumption and glycogen synthesis and enhanced the activities of pyruvate kinase and hexokinase in IR-HepG2 cells. Moreover, RLMP significantly increased the activities of antioxidant enzymes, such as CAT, SOD, and GSH-Px and decreased those of ROS and MDA. Western blotting analysis confirmed that RLMP enhances glucose and lipid metabolism and reduces oxidative stress by activating the PI3K/Akt/GLUT-4 signaling pathway, thereby exerting its hypoglycemic effect. These findings suggest that RLMP is a promising candidate for developing novel antioxidant agents or hypoglycemic drugs.
危险品化学品经营许可证（带存储）