International Journal of Electrochemical Science
·
2023
·
IF 1.5
Nian Lu,Wen-chang Wang,Xiao-hui Chen,Zhi-dong Chen
In this paper, a PSA/Ru(bpy) 3 2+ modified electrode was prepared by electrochemical polymerization and used as the working electrode in ECL detection of melamine. Under the optimal conditions, the sensor shows a wide linear range of 1.0×10 -7 ~1.0×10 -5 mol/L, detection limit of 2.5×10 -8 mol/L with good reproducibility and stability. The method is expected to be a new method for the detection of melamine, which is simple and convenient.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 16
·5198-5203
·
IF 4.616
Shimeles Addisu Kitte,Fuad Abduro Bushira,Haijuan Li,Yongdong Jin
Thioacetamide (TAA) is a well-known hepatotoxic substance, so it is important to determine its presence and content in food and environmental samples. Herein, we report a highly sensitive determination method for TAA based on the electrochemiluminescence (ECL) of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) for the first time by using TAA as a new coreactant for Ru(bpy)32+ ECL via an anodic route. The developed Ru(bpy)32+–TAA ECL system allows the determination of TAA with a good dynamic linear range and low limit of detection (LOD) of 0.1 μM to 1000 μM and 0.035 μM (3σ/m), respectively. In addition, the established ECL system can be applied to detect TAA in fruit juice and waste water samples with outstanding recoveries.
研究领域:
分析化学
·
环境科学
·
毒理学
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DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Kai Zheng,Jiangfei Pan,Zipei Yu,Changqing Yi,Mei-Jin Li
We have proposed a signal dual-amplification electrochemiluminescence (ECL) strategy based on tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) as chromophores confined with three-dimensional (3D) zinc oxalate metal−organic frameworks (Ru(bpy) 3 2+ @MOFs) for the detection of miRNA-21. The three-dimensional chromophore connectivity in zinc oxalate MOFs provided a network among Ru(bpy) 3 2+ units, shielding the chromophores from solvent molecules and resulting in high Ru(II) complex emission efficiency. Additionally, we discovered that magnetic beads (MBs) used as carrier for enriched signals contribute to enhanced ECL intensity of the chromophore. To evaluate its clinical application, we applied this method to determine the concentration of miRNA-21 solutions ranging from 1.56 to 100 nM, obtaining a calibration curve of ECL intensity versus logarithm of concentration (logC) of miRNA-21 with a high correlation coefficient. This work demonstrates the construction of a signal amplification strategy ECL biosensor for miRNA using Ru(bpy) 3 2+ @MOF systems and its application in ECL detection for analyte methodology.
研究领域:
生物化学
·
分子生物学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Xianda Han,Zhigang Yu,Yangkejia Xiang,Fengqin Li,Xiaoping Tan,Lan Xu,Guiling Zhang
An electrochemical fabrication method of Ru[bpy] 3 2+ @Cu-MOF(-SH)/Au (MOF = metal–organic framework) was developed with a photo/electrochemical information characterization and a DNA-based biosensing validation for the proposed interface. Applying an anodic synthesis method, a modification layer of Ru[bpy] 3 2+ encapsulated with MOF was formed on the Au electrode surface to fabricate an interface with photo/electrochemical dual-signal response. Combining the scanning electrochemical microscopy (SECM) imaging technology with electrochemical and electrochemiluminescence (ECL) detection, the information about the interfacial photo/electrochemical bi-functional properties was explored from three aspects of the parameter variable, intensity variable, and morphology variable. The results show that the effect of parameter variables on the interfacial response follows an “interference effect” mechanism. Under the optimized conditions, the background and the saturation ECL intensities are 610.0 and 24.0 with a quenching efficiency of 96.1% and a detection limit of 30 fM. The saturation current is 48.8 µA with a detection limit of 330.0 nM. Both ECL and electrochemical imaging methods can corroborate each other, validating the logical correlation between the interface fabrication and the response performance in the regional interfacial distribution and laying a methodological foundation for evaluating the interface fabrication quality based on imaging morphology qualitatively. The proposed interface can construct photo/electrochemical dual-signal DNA-based biosensors.
研究领域:
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jin Wang,Xinyu Lu,Huafeng Wang,Yuan Zhong,Zhihui Dai,Tianxiang Wei
In this work, Ru(bpy) 3 2+ -loaded gold nanocage (AuNCs) (Ru–AuNCs) was prepared and found to display a distinct property of electrochemiluminescence (ECL) enhancement under mercury ions (Hg 2+ ) interaction. Based on this, we designed a screen-printed bipolar electrode-ECL (SPBPE-ECL) sensing platform by coupling with the thymine-Hg 2+ -thymine (T-Hg 2+ -T) binding pattern for one-step highly sensitive detection of Hg 2+ . This ECL sensor showed a wide linear detection range (0.75 – 850 μg L −1 ) and low detection limit (0.1290 μg L −1 ) toward Hg 2+ , with a one-step detection procedure and disposable feature, displaying potential applicability in the point-of-care-testing (POCT) of Hg 2+ in the environment. In addition, the Hg 2+ -mediated ECL signal enhancement mechanism of Ru–AuNCs was also investigated. It was confirmed that Hg 2+ interaction etched the cage structure of Ru–AuNCs, which sped up the release of more Ru(bpy) 3 2+ around the sensing electrode. Furthermore, Au–Hg alloy structure was formed on the surface of Ru–AuNCs, which also improved the ECL signal. This target-induced in-situ sensing material surface reconstruction strategy would provide a better design concept for the construction of ECL POCT sensor.
研究领域:
环境科学
·
分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Li Jingxian,Shan Xueling,Jiang Ding,Wang Wenchang,Chen Zhidong
Ultrasensitive and selective determination of bisphenol A (BPA) was achieved by a solid-state electrochemiluminescence (ECL) aptasensor. The Ti-based MOFs (MIL-125, MIL stands for Materials of Institute Lavoisier) have porous structure and large specific surface area. They were used as a carrier for Ru(bpy) 3 2+ by a hydrothermal synthesis method. The Ru(bpy) 3 2+ encapsulated MIL-125 (Ru(bpy) 3 2+ @MIL-125) was dropped on a glass carbon electrode (GCE) and used as working electrode. After that, the thiol-based aptamer of BPA was used to modify the working electrode by Ti–S bond. As a result of the specific binding affinity between aptamer and BPA, the ECL signal was significantly quenched. Thus, a selective electrochemiluminescence (ECL) aptasensor for BPA was developed. The ECL aptasensor based on Ru(bpy) 3 2+ @MIL-125 has a good ECL response toward detecting BPA. Under optimal conditions, the aptasensor can detect BPA in a wide linear range from 1.0 × 10 −12 to 1.0 × 10 −6 M with an excellent determination limit of 6.1 × 10 −13 M (S/ N = 3). The ECL aptasensor can selectively detect BPA in a mixture containing BPA interference compounds.
研究领域:
分析化学
·
环境科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yingjie Zhao,Li Tian,Xin Zhang,Zhuo Sun,Xiangyu Shan,Qian Wu,Ruizhan Chen,Juan Lu
A specificity recognition molecularly imprinted electrochemiluminescence sensor (MIECLS) for determination of clenbuterol (CLB) was established by molecular imprinting technology. In this sensing platform, Fe 2 O 3 was used as the carrier to fix Ru(bpy) 3 2+ , and it could also improve electrochemiluminescence (ECL) signal due to large specific surface area and better electronic transmission efficiency. The introduction of molecular imprinted membranes (MIPs) with CLB as template molecules realized the specificity identification of sensors. The intensity of the ECL signal decreased gradually when the concentration of CLB increased. Based on this characteristic, a novel method for measuring CLB was established, and the related mechanism was studied. Under the optimal conditions, the ECL response was proportional to the logarithm of CLB concentration in the range of 1 × 10 −9 mol L −1 to 1 × 10 −4 mol L −1 with a detection limit of 3.3 × 10 −10 mol L −1 . The results prove that MIECLS has great potential in pork and liver samples testing.
研究领域:
分析化学
·
生物传感
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Huiling Li,Li Tian,Shuning Yang,Chao Li,Ruidan Li,Yujia Song,Yanjia Guo,Xuwen Li
To sensitively monitor trace-level of malathion (MAT) in vegetable samples, an ultrasensitive solid-state electrochemiluminescence (ECL) sensor was proposed based on TiO 2 @CdSe and Ru(bpy) 3 2+ @Ag NPs. In this system, the introduction of Ag NPs enhanced the initial ECL signal of Ru(bpy) 3 2+ - tripropylamine (TPrA). When TiO 2 @CdSe was introduced into the system, the ECL signal was further enhanced, which may be due to the synergistic effect of the two complexes. Based on the fact that the addition of MAT causes ECL strength to decrease, a ECL sensor for the ultrasensitive trace detection of MAT has been successfully established. Under the optimized conditions, a wide liner detection range of MAT concentrations from 4.0 × 10 −14 to 4.0 × 10 −9 mol L −1 and a low detection limit (S/N = 3) of 1.3 × 10 −14 mol L −1 were obtained. The ECL sensor has been applied to the detection of vegetable samples and satisfactory results were obtained.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2014
·
IF 3.511
Xiaomei Chen,Limin Zhao,Xiaotian Tian,Sai Lian,Zhiyong Huang,Xi Chen
A novel method for the determination of tetracyclines (TCs) using electrochemiluminescence (ECL) based on a Ru(bpy) 3 2+ -doped silica nanoparticles (RuSiNPs)/Nafion film modified electrode is presented in this paper. The RuSiNPs were prepared by a water-in-oil microemulsion method. The characterization results indicated that the thus-prepared RuSiNPs presented a uniform size of 45 nm and retained the original electrochemical properties of Ru(bpy) 3 2+ . Importantly, the ECL response on RuSiNPs/Nafion film modified electrode was greatly enhanced by TCs. Under the optimum conditions, the ECL intensity versus the concentration of TCs was found to be linear over the range of 1–100 μmol L −1 for tetracycline (TC), 0.1–100 μmol L −1 for oxytetracycline (OTC) and 1–100 μmol L −1 for chlortetracycline (CTC). The detection limits ( S / N =3) were 0.23 μmol L −1 for TC, 0.10 μmol L −1 for OTC and 0.16 μmol L −1 for CTC. Moreover, due to the electrostatic interaction between Ru(bpy) 3 2+ and silica matrix, the leaching of Ru(bpy) 3 2+ was greatly reduced, therefore, the ECL sensor exhibited excellent repeatability and stability in the detection of TCs. Based on these investigations, the proposed ECL approach was successfully used to analyze the TCs content in drugs.
研究领域:
分析化学
·
药物分析
·
生物传感
✓ 已验证
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 80
·50634-50642
·
IF 3.108
Lijun Luo,Libo Li,Xixi Xu,Dong Liu,Jinyang Li,Kun Wang,Tianyan You
Sensitive and quantitative analysis of pentachlorophenol (PCP) is especially important in the field of ecology and agriculture. Herein, a highly sensitive and stable Ru(bpy)32+-based anodic electrochemiluminescence (ECL) system utilizing nitrogen-doped graphene quantum dots (NGQDs) as a novel co-reactant was firstly constructed to detect PCP. In this system, Ru(bpy)32+ was used as luminophor, while NGQDs were applied as co-reactant in place of the toxic and volatile tripropylamine. The novel co-reactant not only promoted the luminous efficiency of Ru(bpy)32+, but also improved the stability of the ECL system. In addition, the enhancement mechanism of NGQDs on Ru(bpy)32+ and quenching mechanism of PCP on Ru(bpy)32+/NGQDs were investigated in detail. Under the optimum conditions, the as-fabricated sensor for ultrasensitive PCP determination expressed a wider linear range of 1 × 10−15 to 1 × 10−5 g mL−1 and a lower detection limit of 2 × 10−17 g mL−1 with good stability and repeatability. Furthermore, based on the distinctive advantages of the novel ECL sensor, it was successfully used to detect PCP in tap water and river water, and satisfactory recoveries were obtained.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xin Zhang,Li Tian,Zhuo Sun,Qian Wu,Xiangyu Shan,Yingjie Zhao,Ruizhan Chen,Juan Lu
A molecular-imprinted electrochemiluminescence sensor for procymidone (PCM) detection was reported based on Ru(bpy) 3 2+ @ZIF-7. A novel self-accelerated Ru(bpy) 3 2+ @ZIF-7 was prepared by one-step synthesis method, which exhibited high electrochemiluminescence (ECL) emission as a coreactant in the presence of tri- n -propylamine (TPrA). Concretely, ZIF-7 was not only the carrier of Ru(bpy) 3 2+ , but also the co-reaction promoter of Ru(bpy) 3 2+ /TPrA ECL system. The introduction of molecular imprint polymer (MIP) with PCM gave new characteristics of specific recognition of analyte PCM. The change value of ECL intensity (ΔI) was proportional to the logarithm of PCM concentration, with a wide linear range and a low detection limit of 1.0 × 10 −10 to 1.0 × 10 −6 mol L −1 and 2.0 × 10 −11 mol L −1 , respectively. The presented MIP-ECL sensor had high sensitivity, selectivity and stability, and had great potential in the field of food safety detection.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Shuning Yang,Li Tian,Weiwei Fu,Huiling Li,Chao Li,Yujia Song,Ruidan Li,Yanjia Guo,Lun Zhao
The novel TiO 2 and Ni-MOF materials were synthesized and utilized for the detection of permethrin (PET). A highly sensitive solid-state electrochemiluminescence (ECL) sensor was developed based on Ni-MOF@Ru(bpy) 3 2+ and Au NPs@TiO 2 . In this sensing platform, Ru(bpy) 3 2+ -Tripropyl Amine (TPrA) was used as a luminescent signal, Ni-MOF acted as a carrier to carry more luminescent reagents Ru(bpy) 3 2+ . Au NPs acted as promoters facilitated electron transport and TiO 2 could further enhance the luminescence intensity of the system by synergistical interaction with Au NPs. The possible mechanisms of signal amplification were investigated. The ECL intensity decreased significantly with increasing PET concentration, enabling the determination of PET amount through the observation of the change in ECL signal intensity (ΔI). Under optimal experimental conditions, the linear range of PET concentration from 1.0 × 10 −11 mol L −1 to 1.0 × 10 −6 mol L −1 , with a detection limit of 3.3 × 10 −12 mol L −1 (3S/N). This method was successfully applied to determine PET in various vegetable samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）