International Journal of Electrochemical Science
·
2023
·
IF 1.5
Qiu-feng Lan,Wen-chang Wang,Xiao-hui Chen,Zhi-dong Chen
The poly(3-amino-4-hydroxybenzenesulfonic acid)/Ru(bpy) 3 2+ modified glassy carbon electrode (PAHBS/Ru(bpy) 3 2+ /GCE) is a novel solid-state electrochemiluminescence (ECL) sensor. It was prepared by electropolymerization of 3-amino-4-hydroxybenzenesulfonicacid (AHBS) followed by soaking into Ru(bpy) 3 2+ solution. Ru(bpy) 3 2+ was entrapped on the electrode via the electrostatic interactions between sulfonic acid groups and Ru(bpy) 3 2+ cation. The morphology and composition of the modified electrodes were analysed using scanning electron microscope and energy-dispersed spectrum. Cyclic voltammetry and electrochemical impedance spectroscopy were used to compare the electrochemical behavior of the electrodes before and after modification. Under the optimal conditions, the ECL intensity of the quenching value (ΔECL) versus the logarithm of the concentration of malachite green (MG) was linear over a concentration range from 5.0 × 10 -8 to 1.0 × 10 -5 mol L -1 ( r = 0.9931) and the limit of detection (LOD) was 2.5 × 10 -8 mol L -1 . This method has been successfully applied to the determination of MG in pond water.
研究领域:
分析化学
·
环境科学
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高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2325-2331
·
IF 4.58
Huang Xinan,Jiang Hong,Li Yongxin,Sang Lijia,Zhou Huipeng,Shahzad Sohail Anjum,Ibupoto Zafar Hussain,Yu Cong
A sensitive and selective luminescent nanoprobe (referred to as DEPN) is designed for the determination of Cu(II) ions. DEPN shows two emission peaks, one at 602 nm and caused by [Ru(bpy) 3 ] 2+ , the other (peaking at 500 nm) caused by silver nanoclusters (AgNCs). The luminescence of the ruthenium complex is inert and acts as the internal reference signal, while that of the AgNCs is quenched by Cu(II) ions. Under 350 nm photoexcitation, a linear relationship is found between the ratio of emission intensity (I 500 /I 602 ) and the concentration of Cu(II) ions in the 0.1–16 μM range. The detection limit of Cu(II) ions is estimated to be 50 nM. The DEPN was applied to the determination of Cu(II) ions in (spiked) water samples, and to intracellular imaging of Cu(II) in HeLa cells using confocal fluorescence microscopy.
Schematic presentation of a dual-emission photoluminescence nanoprobe for selective Cu 2+ sensing. The emission of the Ag nanoclusters (at 500 nm) is quenched by Cu 2+ , while that of the Ru(bpy) 3
2+ -doped silica nanoparticles (at 602 nm) remains unchanged and serves as a reference.
研究领域:
纳米技术
·
分析化学
·
材料科学
·
生物医学工程
·
细胞生物学
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jin Wang,Xinyu Lu,Huafeng Wang,Yuan Zhong,Zhihui Dai,Tianxiang Wei
In this work, Ru(bpy) 3 2+ -loaded gold nanocage (AuNCs) (Ru–AuNCs) was prepared and found to display a distinct property of electrochemiluminescence (ECL) enhancement under mercury ions (Hg 2+ ) interaction. Based on this, we designed a screen-printed bipolar electrode-ECL (SPBPE-ECL) sensing platform by coupling with the thymine-Hg 2+ -thymine (T-Hg 2+ -T) binding pattern for one-step highly sensitive detection of Hg 2+ . This ECL sensor showed a wide linear detection range (0.75 – 850 μg L −1 ) and low detection limit (0.1290 μg L −1 ) toward Hg 2+ , with a one-step detection procedure and disposable feature, displaying potential applicability in the point-of-care-testing (POCT) of Hg 2+ in the environment. In addition, the Hg 2+ -mediated ECL signal enhancement mechanism of Ru–AuNCs was also investigated. It was confirmed that Hg 2+ interaction etched the cage structure of Ru–AuNCs, which sped up the release of more Ru(bpy) 3 2+ around the sensing electrode. Furthermore, Au–Hg alloy structure was formed on the surface of Ru–AuNCs, which also improved the ECL signal. This target-induced in-situ sensing material surface reconstruction strategy would provide a better design concept for the construction of ECL POCT sensor.
研究领域:
环境科学
·
分析化学
·
生物传感
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Li Jingxian,Shan Xueling,Jiang Ding,Wang Wenchang,Chen Zhidong
Ultrasensitive and selective determination of bisphenol A (BPA) was achieved by a solid-state electrochemiluminescence (ECL) aptasensor. The Ti-based MOFs (MIL-125, MIL stands for Materials of Institute Lavoisier) have porous structure and large specific surface area. They were used as a carrier for Ru(bpy) 3 2+ by a hydrothermal synthesis method. The Ru(bpy) 3 2+ encapsulated MIL-125 (Ru(bpy) 3 2+ @MIL-125) was dropped on a glass carbon electrode (GCE) and used as working electrode. After that, the thiol-based aptamer of BPA was used to modify the working electrode by Ti–S bond. As a result of the specific binding affinity between aptamer and BPA, the ECL signal was significantly quenched. Thus, a selective electrochemiluminescence (ECL) aptasensor for BPA was developed. The ECL aptasensor based on Ru(bpy) 3 2+ @MIL-125 has a good ECL response toward detecting BPA. Under optimal conditions, the aptasensor can detect BPA in a wide linear range from 1.0 × 10 −12 to 1.0 × 10 −6 M with an excellent determination limit of 6.1 × 10 −13 M (S/ N = 3). The ECL aptasensor can selectively detect BPA in a mixture containing BPA interference compounds.
研究领域:
分析化学
·
环境科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xin Zhang,Li Tian,Zhuo Sun,Qian Wu,Xiangyu Shan,Yingjie Zhao,Ruizhan Chen,Juan Lu
A molecular-imprinted electrochemiluminescence sensor for procymidone (PCM) detection was reported based on Ru(bpy) 3 2+ @ZIF-7. A novel self-accelerated Ru(bpy) 3 2+ @ZIF-7 was prepared by one-step synthesis method, which exhibited high electrochemiluminescence (ECL) emission as a coreactant in the presence of tri- n -propylamine (TPrA). Concretely, ZIF-7 was not only the carrier of Ru(bpy) 3 2+ , but also the co-reaction promoter of Ru(bpy) 3 2+ /TPrA ECL system. The introduction of molecular imprint polymer (MIP) with PCM gave new characteristics of specific recognition of analyte PCM. The change value of ECL intensity (ΔI) was proportional to the logarithm of PCM concentration, with a wide linear range and a low detection limit of 1.0 × 10 −10 to 1.0 × 10 −6 mol L −1 and 2.0 × 10 −11 mol L −1 , respectively. The presented MIP-ECL sensor had high sensitivity, selectivity and stability, and had great potential in the field of food safety detection.
研究领域:
分析化学
·
食品安全检测
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Hu Yunxia,Su Luyao,Wang Sui,Guo Zhiyong,Hu Yufang,Xie Hongzhen
A method is described for combined magnetic solid phase microextraction and electrochemiluminescent (ECL) detection of the antibiotic tetracycline. A nanocomposite of type Fe 3 O 4 @SiO 2 @ZnO was used as the sorbent. Tetracycline has a strong affinity for Zn(II) ion and therefore is well extracted by this sorbent. The loaded sorbent can be magnetically removed. The extraction efficiency at a 1.0 μM tetracycline concentration is around 88%. Detection is based on the use of carbon nanodots that were prepared form urea and ethylenediaminetetraacetic acid as raw materials. The electrochemical probe Ru(bpy) 3 2+ was added to generate double ECL when scanning the potential between −3.5 and + 2 V. The two ECL signals decreased with the increase of tetracycline concentration. Under optimized experimental conditions, the ratio of the two signals is linearly related to the logarithm of the tetracycline concentration in the range from 1.0 nM to 0.1 mM, with a 0.47 nM detection limit. The method was successfully applied to the determination of tetracycline in spiked milk. It exhibited good sensitivity, selectivity and accuracy due to ratiometric read-out and prior preconcentration of analyte.
Tetracycline (TC) has a strong affinity for Zn(II) ion and is well extracted by the Fe 3 O 4 @SiO 2 @ZnO nanocomposite. Ru(bpy) 3 2+ can generate double electrochemiluminescence signals based on the use of carbon nanodots (C-dots) as coreactant. The two signals decrease with the increase of tetracycline concentration. [Ru(bpy) 3 2+ ]* stands for excited state Ru(bpy) 2 2+ .
研究领域:
分析化学
·
环境科学
·
药物分析
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yingjie Zhao,Li Tian,Xin Zhang,Zhuo Sun,Xiangyu Shan,Qian Wu,Ruizhan Chen,Juan Lu
A specificity recognition molecularly imprinted electrochemiluminescence sensor (MIECLS) for determination of clenbuterol (CLB) was established by molecular imprinting technology. In this sensing platform, Fe 2 O 3 was used as the carrier to fix Ru(bpy) 3 2+ , and it could also improve electrochemiluminescence (ECL) signal due to large specific surface area and better electronic transmission efficiency. The introduction of molecular imprinted membranes (MIPs) with CLB as template molecules realized the specificity identification of sensors. The intensity of the ECL signal decreased gradually when the concentration of CLB increased. Based on this characteristic, a novel method for measuring CLB was established, and the related mechanism was studied. Under the optimal conditions, the ECL response was proportional to the logarithm of CLB concentration in the range of 1 × 10 −9 mol L −1 to 1 × 10 −4 mol L −1 with a detection limit of 3.3 × 10 −10 mol L −1 . The results prove that MIECLS has great potential in pork and liver samples testing.
研究领域:
分析化学
·
生物传感
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Li Tian,Xuerong Wang,Kexin Wu,Yue Hu,Yue Wang,Juan Lu
A ultrasensitive electrochemiluminescence (ECL) biosensor was designed for the detection of dopamine using ZnSe quantum dots and Ru(bpy) 3 2+ as the signal probes, and graphite oxide and multi-walled carbon nanotube as the supporting matrix. The biosensor exhibits efficient and stable anodic ECL signal (at 1.10 V vs. Ag/AgCl) in phosphate buffer solution (pH 9.0) containing Tripropyl amine. The ECL emission decreases with adding dopamine, which can be attributed to the quenching effect from interaction between ZnSe and dopamine. The possible reaction mechanism of the ECL system is studied, and a method for the determination of dopamine is proposed. Under the optimal experimental conditions, the ECL emission decreases linearly with the logarithm of dopamine concentration in a wide linear range from1 × 10 −9 to 1 × 10 -5 mol L −1 with a detection limit of 6 × 10 -10 mol L -1 (S/N = 3). The biosensor is also successfully applied to detect dopamine in human blood serum samples.
研究领域:
生物传感
·
分析化学
·
材料科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Xiaojian Li,Yaoguang Wang,Li Shi,Hongmin Ma,Yong Zhang,Bin Du,Dan Wu,Qin Wei
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy) 3 2+ /silica/Fe 3 O 4 nanomaterials (CRuSi-Fe 3 O 4 ) were synthesized through W/O microemulsion route. The added Fe 3 O 4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe 3 O 4 . In addition, the layered structure of CRuSi-Fe 3 O 4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo 2 S 4 nanoparticles-grown on carboxylic graphene (NiCo 2 S 4 -COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo 2 S 4 was used to quench the ECL of CRuSi-Fe 3 O 4 , graphene was used to grow NiCo 2 S 4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S 2 O 8 2- as the coreactant of CRuSi-Fe 3 O 4 . Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL −1 to 100 ng mL −1 was obtained, and the detection limit was calculated to be 0.18 pg mL −1 (S/N=3).
研究领域:
生物传感
·
材料科学
·
分析化学
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DOI
PubMed
高影响因子
CHINESE SCIENCE BULLETIN
·
2013
·Vol. 59
·期 2
·147-153
·
IF 1.319
Wang Wei,Yan Xiao Li,Zhan Lei,Leng Fei,Yang Xiao Xi,Huang Cheng Zhi
A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris(2,2′-bipyridyl)ruthenium(II)@SiO 2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)
2+ 3
) as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru(bpy)
2+ 3
@SiO 2 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt-Ru(bpy)
2+ 3
@SiO 2 NPs had great tendency to human bone marrow neuroblastoma cells (SK-N-SH cells), since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.
研究领域:
纳米技术
·
生物医学工程
·
细胞生物学
·
分子生物学
·
荧光成像
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xueqing Gao,Xiaowen Gu,Qi Min,Yueyue Wei,Chunyuan Tian,Xuming Zhuang,Feng Luan
In this work, we demonstrate a novel solid-state electrochemiluminescence (ECL) sensor based on the Ru(bpy) 3 2+ @terbium-guanosine monophosphate infinite coordination polymer network ((Ru(bpy) 3 2+ @Tb-GMP ICPn). Comparing with the traditional luminescence of Ru(bpy) 3 2+ observed in a liquid system, the proposed method of encapsulating Ru(bpy) 3 2+ into ICPn for immobilization greatly improves the ECL signal and efficiency, which is attributed to the unique porous structure and large specific surface area of ICPn. Moreover, the solid-state Ru(bpy) 3 2+ ECL sensor has good biocompatibility and low toxicity. Taking histamine (HA) as a detection model, a good linear relationship between ECL intensity and logarithm of HA concentration was obtained with a low detection limit of 17 nM, and satisfactory results were obtained for detecting HA levels in fish samples as well. The proposed solid-state Ru(bpy) 3 2+ ECL sensor has great application prospects in the safety of food.
研究领域:
分析化学
·
食品科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Kaida Kuang,Ya Li,Yu Ji,Yang Liu,Nengqin Jia
In this work, a quenched molecularly imprinting-electrochemiluminescence (MIP-ECL) sensor based on Ru(bpy) 3 2+ @ZnO-Au nanocomposites was constructed to realize the sensitive detection of acrylamide (AM). Ru(bpy) 3 2+ @ZnO-Au nanomaterials show high ECL efficiency. AuNPs has excellent properties and can improve the properties of electrochemiluminescence by promoting the electron transfer process of the luminescent body. The MIP polymer film was formed on the surface of the material and eluted with eluent, leaving an AM imprinting cavity as a specific recognition site. When adding different concentrations of AM, it was specifically recognized by the MIP membrane, and the ECL signal decreased gradually, thus realizing the sensitive detection of AM. Under the optimal conditions, the detection ranges of the MIP-ECL sensor are 1–10 8 nM and the limit of detection (LOD) is 0.123 nM. Therefore, this sensor proposed in this work opens up a new way for the sensitive detection of AM in food.
研究领域:
分析化学
·
食品安全检测
·
纳米材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）