ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Wenwu Li,Tingting Xie,Shizhen Gan,Yuanxun Gong,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao
Background Electrochemiluminescence (ECL) biosensors are powerful tools for biomarker detection due to high sensitivity, low background, and programmable signal generation. However, conventional ECL platforms face limitations including reliance on static recognition elements (e.g., antibodies) and susceptibility to denaturation. To address this, we developed a novel β-turn-structured peptide-based ECL biosensor integrating MXene (Ti3C2) nanomaterials with a Ru(dcbpy)32+/AuNPs hybrid. This system targets SERPINE1, a senescence-associated secretory protein and promising early biomarker for lung cancer, leveraging peptide conformational switching for enzyme-free signal amplification. Results The biosensor employs a rationally engineered peptide probe containing: (1) a SERPINE1-specific recognition motif, (2) a central β-turn domain locking the peptide in a catalytically inactive folded state, and (3) a His/Cys-rich tail mimicking peroxidase-like activity. Target binding unfolds the β-turn structure, exposing catalytic residues that accelerate tripropylamine (TPA) oxidation and enhance ECL emission. MXene provides a high-conductivity platform while facilitating efficient peptide immobilization via embedded AuNPs. Under optimized conditions, the sensor achieves ultrasensitive detection of SERPINE1 with a wide linear range (0.05–800 ng/mL), a low detection limit (11.2 pg/mL), and high specificity. It operates without enzymatic amplification and demonstrates excellent reproducibility (RSD = 4.2%) and stability (>85% signal retention after 8 days). Validation in human serum yielded recoveries of 96.3–104.6%. Significance This work introduces the first MXene-assisted ECL biosensor utilizing β-turn peptide conformational switching. The enzyme-free, modular strategy offers clinically adaptable detection of senescence biomarkers for early lung cancer diagnosis. By integrating molecular recognition, structural dynamics, and nanomaterial-enhanced signal transduction, this platform advances programmable biosensing and can be extended to other disease-related targets through peptide redesign, showing broad potential in precision diagnostics.
研究领域:
生物传感器
·
纳米技术
·
生物化学
·
癌症诊断
·
电化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Qingqing Huang,Fang Luo,Cuiying Lin,Jian Wang,Bin Qiu,Zhenyu Lin
Ru(dcbpy) 3 2+ -polyethyleneimine-L-lysine (Ru-PEI-L-lys) had been immobilized on metal organic frameworks (ZIF-8) to form an electrochemiluminescent(ECL) indicator (Ru-PEI-L-lys-ZIF-8). In this ECL indicator, PEI-L-lys is used as a co-reactant. Platinum nanoparticles (PtNPs) has been mixed with Ru-PEI-L-lys-ZIF-8 to form a thin film to increase the electron transfer rate and enhanced the ECL response of the system. The prepared material had been characterized carefully and been combined with high selectivity of aptamer to develop a ECL biosensor for thrombin detection. RecJ f exonuclease (an ssDNA specific exonuclease) assistant target recycling amplification has been adopted to enhance the sensitivity of the system. The ECL response of the system has a linear relationship with logarithm of thrombin concentration in the range of 1 fM to 10 pM with a detection limit of 0.02 aM. This work not only provides a new strategy for the design and synthesis of high performance and stable ECL indicator, but also opens up a new approach for the development of highly sensitive ECL sensors for biological analysis.
研究领域:
分析化学
·
生物化学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Qian Long,Hanfeng Cui,Jing Zhang,Qiqi Fan,Ting Huang,Hai Shuai,Xiaocui Yin,Guobing Wei,Huanwen Chen,Hao Fan
Cu 2+ serves as a significant biomarker in various physiological processes. This study presents the development of a novel Ru-UiO-TPEA functionalized metal-organic framework (MOFs) probe for Cu 2+ detection, employing both electrochemiluminescence (ECL) and electrochemical (EC) methods. Employing a dual ligand-assisted energy transfer approach, we synthesized Ru-UiO with optimal ECL and EC properties by adjusting the synthesis ratio of biphenyldicarboxylic acid to Ru(dcbpy) 3 2+ . This was followed by its conjugation with N-(2-aminoethyl)-N, N′, N′-tris(pyridin-2-ylmethyl) ethane-1,2-diamine (AE-TPEA) to form the highly conjugated Ru-UiO-TPEA probe for Cu 2+ detection. As the Cu 2+ concentration increased, the probe exhibited a diminished ECL signal and an enhanced differential pulse voltammetry (DPV) signal. The dual-mode detection method enabled cross-validation of the ECL and EC signals, enhancing accuracy and effectively minimizing errors. The ECL and EC methods had detection limits of 0.26 nM (range: 1 nM to 10 μM) and 0.65 nM (range: 2 nM to 80 μM), respectively. Meanwhile, these two methods can effectively detect Cu 2+ in rat brain microdialysis fluid. Notably, the measured concentrations correlated well with those determined by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES), highlighting its potential in biological and clinical bioelectronic sensing applications.
研究领域:
分析化学
·
生物化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Mengyang Zhou,Yifei Wang,Yaning Xia,Yinhua Li,Jianfeng Bao,Yong Zhang,Jingliang Cheng,Yupeng Shi
Nanotechnology for tumor diagnosis and optical therapy has attracted widespread interest due to its low toxicity and convenience but is severely limited due to uncontrollable tumor targeting. In this work, homologous cancer cell membrane-camouflaged multifunctional hybrid metal coordination nanoparticles (DRu/Gd@CM) were prepared for MRI-guided photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors. Bimetallic coordination nanoparticles are composed of three functional modules: dopamine, Ru(dcbpy) 3 Cl 2 and GdCl 3 , which are connected through 1,4-Bis[(1H-imidazole-1-yl)methyl]benzene (BIX). Their morphology can be easily controlled by adjusting the ratio of precursors. Optimistically, the intrinsic properties of the precursors, including the photothermal properties of polydopamine (PDA), the magnetic resonance (MR) response of Gd 3+ , and the singlet oxygen generation of Ru(dcbpy) 3 Cl 2 , are well preserved in the hybrid metal nanoparticles. Furthermore, the targeting of homologous cancer cell membranes enables these coordinated nanoparticles to precisely target tumor cells. The MR imaging capabilities and the combination of PDT and PTT were demonstrated in in vitro experiments. In addition, in vivo experiments indicated that the nanoplatform showed excellent tumor accumulation and therapeutic effects on mice with subcutaneous tumors, and could effectively eliminate tumors within 14 days. Therefore, it expanded the new horizon for the preparation of modular nanoplatform and imaging-guided optical therapy of tumors.
研究领域:
纳米技术
·
生物医学工程
·
癌症治疗
·
分子成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）