Qingqing Huang,Fang Luo,Cuiying Lin,Jian Wang,Bin Qiu,Zhenyu Lin
Ru(dcbpy) 3 2+ -polyethyleneimine-L-lysine (Ru-PEI-L-lys) had been immobilized on metal organic frameworks (ZIF-8) to form an electrochemiluminescent(ECL) indicator (Ru-PEI-L-lys-ZIF-8). In this ECL indicator, PEI-L-lys is used as a co-reactant. Platinum nanoparticles (PtNPs) has been mixed with Ru-PEI-L-lys-ZIF-8 to form a thin film to increase the electron transfer rate and enhanced the ECL response of the system. The prepared material had been characterized carefully and been combined with high selectivity of aptamer to develop a ECL biosensor for thrombin detection. RecJ f exonuclease (an ssDNA specific exonuclease) assistant target recycling amplification has been adopted to enhance the sensitivity of the system. The ECL response of the system has a linear relationship with logarithm of thrombin concentration in the range of 1 fM to 10 pM with a detection limit of 0.02 aM. This work not only provides a new strategy for the design and synthesis of high performance and stable ECL indicator, but also opens up a new approach for the development of highly sensitive ECL sensors for biological analysis.
Cu 2+ serves as a significant biomarker in various physiological processes. This study presents the development of a novel Ru-UiO-TPEA functionalized metal-organic framework (MOFs) probe for Cu 2+ detection, employing both electrochemiluminescence (ECL) and electrochemical (EC) methods. Employing a dual ligand-assisted energy transfer approach, we synthesized Ru-UiO with optimal ECL and EC properties by adjusting the synthesis ratio of biphenyldicarboxylic acid to Ru(dcbpy) 3 2+ . This was followed by its conjugation with N-(2-aminoethyl)-N, N′, N′-tris(pyridin-2-ylmethyl) ethane-1,2-diamine (AE-TPEA) to form the highly conjugated Ru-UiO-TPEA probe for Cu 2+ detection. As the Cu 2+ concentration increased, the probe exhibited a diminished ECL signal and an enhanced differential pulse voltammetry (DPV) signal. The dual-mode detection method enabled cross-validation of the ECL and EC signals, enhancing accuracy and effectively minimizing errors. The ECL and EC methods had detection limits of 0.26 nM (range: 1 nM to 10 μM) and 0.65 nM (range: 2 nM to 80 μM), respectively. Meanwhile, these two methods can effectively detect Cu 2+ in rat brain microdialysis fluid. Notably, the measured concentrations correlated well with those determined by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES), highlighting its potential in biological and clinical bioelectronic sensing applications.
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