Arginine has been widely applied in the food industry as coloring agents, flavoring agents, and nutritional fortifiers. It is also one of the major components of feed additives. Currently, methods for the highly selective detection of arginine remain absent. For accurate and sensitive detection of L−arginine, a novel ratiometric fluorescence assay based on Ru@UiO-66-NH2was developed and demonstrated in this study. Under optimized detection conditions, the limit of detection (LOD) of this assay for L-arginine was 2.32 μM, which is superior to most assays reported to date. Meanwhile, Ru@UiO-66-NH2showed good stability within 30 days, demonstrating the wide applicability of the proposed assay. The spike-and-recovery rates of the proposed assay for L-arginine in real samples (e.g., tea, grape juice, and serum) were 84.27–113.09%. Overall, the proposed assay showed high sensitivity, good reproducibility, and excellent stability in the detection of L-arginine in both buffer and real samples.
Pei Jia,Kairong Yang,Jinjie Hou,Yuanyuan Cao,Xin Wang,Li Wang
The increasing deterioration of ecosystem derived from heavy metals residues brings about the environmental and food contamination , which presses the exploration of facile platform for monitoring heavy metals. Herein, a ratiometric fluorescence sensor was designed for Hg 2+ detection based on the compound of UiO-66-NH 2 and Ru(bpy) 3 2+ (Ru@UiO-66-NH 2 ) which was synthesized by situ encapsulation. The innovative composite displayed two emission peaks at 437 and 604 nm, and the addition of Hg 2+ could only quench the blue fluorescence due to static quenching and photo-induced electron transfer mechanism, providing an internal standard to promote the precision. Under optimal conditions, the ratiometric Ru@UiO-66-NH 2 probe revealed outstanding anti-interference capability and performed with a great limit of detection (LOD) of 0.053 μM for Hg 2+ , which was 2-fold lower than that of single-color UiO-66-NH 2 . By merit of Ru@UiO-66-NH 2 , test hydrogels were fabricated to provide a tactics for visual, rapid and on-site detection of Hg 2+ . Additionally, the dual-emitting sensing platform presented satisfactory recoveries and reliabilities in lake water, tap water , and drink water, demonstrating the application potential of this proposed ratiometric fluorescence sensor for monitoring Hg 2+ .
危险品化学品经营许可证（带存储）