ANALYST
·
2021
·Vol. 146
·期 16
·5198-5203
·
IF 4.616
Shimeles Addisu Kitte,Fuad Abduro Bushira,Haijuan Li,Yongdong Jin
Thioacetamide (TAA) is a well-known hepatotoxic substance, so it is important to determine its presence and content in food and environmental samples. Herein, we report a highly sensitive determination method for TAA based on the electrochemiluminescence (ECL) of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) for the first time by using TAA as a new coreactant for Ru(bpy)32+ ECL via an anodic route. The developed Ru(bpy)32+–TAA ECL system allows the determination of TAA with a good dynamic linear range and low limit of detection (LOD) of 0.1 μM to 1000 μM and 0.035 μM (3σ/m), respectively. In addition, the established ECL system can be applied to detect TAA in fruit juice and waste water samples with outstanding recoveries.
研究领域:
分析化学
·
环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Kai Zheng,Jiangfei Pan,Zipei Yu,Changqing Yi,Mei-Jin Li
We have proposed a signal dual-amplification electrochemiluminescence (ECL) strategy based on tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) as chromophores confined with three-dimensional (3D) zinc oxalate metal−organic frameworks (Ru(bpy) 3 2+ @MOFs) for the detection of miRNA-21. The three-dimensional chromophore connectivity in zinc oxalate MOFs provided a network among Ru(bpy) 3 2+ units, shielding the chromophores from solvent molecules and resulting in high Ru(II) complex emission efficiency. Additionally, we discovered that magnetic beads (MBs) used as carrier for enriched signals contribute to enhanced ECL intensity of the chromophore. To evaluate its clinical application, we applied this method to determine the concentration of miRNA-21 solutions ranging from 1.56 to 100 nM, obtaining a calibration curve of ECL intensity versus logarithm of concentration (logC) of miRNA-21 with a high correlation coefficient. This work demonstrates the construction of a signal amplification strategy ECL biosensor for miRNA using Ru(bpy) 3 2+ @MOF systems and its application in ECL detection for analyte methodology.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Xianda Han,Zhigang Yu,Yangkejia Xiang,Fengqin Li,Xiaoping Tan,Lan Xu,Guiling Zhang
An electrochemical fabrication method of Ru[bpy] 3 2+ @Cu-MOF(-SH)/Au (MOF = metal–organic framework) was developed with a photo/electrochemical information characterization and a DNA-based biosensing validation for the proposed interface. Applying an anodic synthesis method, a modification layer of Ru[bpy] 3 2+ encapsulated with MOF was formed on the Au electrode surface to fabricate an interface with photo/electrochemical dual-signal response. Combining the scanning electrochemical microscopy (SECM) imaging technology with electrochemical and electrochemiluminescence (ECL) detection, the information about the interfacial photo/electrochemical bi-functional properties was explored from three aspects of the parameter variable, intensity variable, and morphology variable. The results show that the effect of parameter variables on the interfacial response follows an “interference effect” mechanism. Under the optimized conditions, the background and the saturation ECL intensities are 610.0 and 24.0 with a quenching efficiency of 96.1% and a detection limit of 30 fM. The saturation current is 48.8 µA with a detection limit of 330.0 nM. Both ECL and electrochemical imaging methods can corroborate each other, validating the logical correlation between the interface fabrication and the response performance in the regional interfacial distribution and laying a methodological foundation for evaluating the interface fabrication quality based on imaging morphology qualitatively. The proposed interface can construct photo/electrochemical dual-signal DNA-based biosensors.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Huiling Li,Li Tian,Shuning Yang,Chao Li,Ruidan Li,Yujia Song,Yanjia Guo,Xuwen Li
To sensitively monitor trace-level of malathion (MAT) in vegetable samples, an ultrasensitive solid-state electrochemiluminescence (ECL) sensor was proposed based on TiO 2 @CdSe and Ru(bpy) 3 2+ @Ag NPs. In this system, the introduction of Ag NPs enhanced the initial ECL signal of Ru(bpy) 3 2+ - tripropylamine (TPrA). When TiO 2 @CdSe was introduced into the system, the ECL signal was further enhanced, which may be due to the synergistic effect of the two complexes. Based on the fact that the addition of MAT causes ECL strength to decrease, a ECL sensor for the ultrasensitive trace detection of MAT has been successfully established. Under the optimized conditions, a wide liner detection range of MAT concentrations from 4.0 × 10 −14 to 4.0 × 10 −9 mol L −1 and a low detection limit (S/N = 3) of 1.3 × 10 −14 mol L −1 were obtained. The ECL sensor has been applied to the detection of vegetable samples and satisfactory results were obtained.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2014
·
IF 3.511
Xiaomei Chen,Limin Zhao,Xiaotian Tian,Sai Lian,Zhiyong Huang,Xi Chen
A novel method for the determination of tetracyclines (TCs) using electrochemiluminescence (ECL) based on a Ru(bpy) 3 2+ -doped silica nanoparticles (RuSiNPs)/Nafion film modified electrode is presented in this paper. The RuSiNPs were prepared by a water-in-oil microemulsion method. The characterization results indicated that the thus-prepared RuSiNPs presented a uniform size of 45 nm and retained the original electrochemical properties of Ru(bpy) 3 2+ . Importantly, the ECL response on RuSiNPs/Nafion film modified electrode was greatly enhanced by TCs. Under the optimum conditions, the ECL intensity versus the concentration of TCs was found to be linear over the range of 1–100 μmol L −1 for tetracycline (TC), 0.1–100 μmol L −1 for oxytetracycline (OTC) and 1–100 μmol L −1 for chlortetracycline (CTC). The detection limits ( S / N =3) were 0.23 μmol L −1 for TC, 0.10 μmol L −1 for OTC and 0.16 μmol L −1 for CTC. Moreover, due to the electrostatic interaction between Ru(bpy) 3 2+ and silica matrix, the leaching of Ru(bpy) 3 2+ was greatly reduced, therefore, the ECL sensor exhibited excellent repeatability and stability in the detection of TCs. Based on these investigations, the proposed ECL approach was successfully used to analyze the TCs content in drugs.
研究领域:
分析化学
·
药物分析
·
生物传感
✓ 已验证
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 80
·50634-50642
·
IF 3.108
Lijun Luo,Libo Li,Xixi Xu,Dong Liu,Jinyang Li,Kun Wang,Tianyan You
Sensitive and quantitative analysis of pentachlorophenol (PCP) is especially important in the field of ecology and agriculture. Herein, a highly sensitive and stable Ru(bpy)32+-based anodic electrochemiluminescence (ECL) system utilizing nitrogen-doped graphene quantum dots (NGQDs) as a novel co-reactant was firstly constructed to detect PCP. In this system, Ru(bpy)32+ was used as luminophor, while NGQDs were applied as co-reactant in place of the toxic and volatile tripropylamine. The novel co-reactant not only promoted the luminous efficiency of Ru(bpy)32+, but also improved the stability of the ECL system. In addition, the enhancement mechanism of NGQDs on Ru(bpy)32+ and quenching mechanism of PCP on Ru(bpy)32+/NGQDs were investigated in detail. Under the optimum conditions, the as-fabricated sensor for ultrasensitive PCP determination expressed a wider linear range of 1 × 10−15 to 1 × 10−5 g mL−1 and a lower detection limit of 2 × 10−17 g mL−1 with good stability and repeatability. Furthermore, based on the distinctive advantages of the novel ECL sensor, it was successfully used to detect PCP in tap water and river water, and satisfactory recoveries were obtained.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Shuning Yang,Li Tian,Weiwei Fu,Huiling Li,Chao Li,Yujia Song,Ruidan Li,Yanjia Guo,Lun Zhao
The novel TiO 2 and Ni-MOF materials were synthesized and utilized for the detection of permethrin (PET). A highly sensitive solid-state electrochemiluminescence (ECL) sensor was developed based on Ni-MOF@Ru(bpy) 3 2+ and Au NPs@TiO 2 . In this sensing platform, Ru(bpy) 3 2+ -Tripropyl Amine (TPrA) was used as a luminescent signal, Ni-MOF acted as a carrier to carry more luminescent reagents Ru(bpy) 3 2+ . Au NPs acted as promoters facilitated electron transport and TiO 2 could further enhance the luminescence intensity of the system by synergistical interaction with Au NPs. The possible mechanisms of signal amplification were investigated. The ECL intensity decreased significantly with increasing PET concentration, enabling the determination of PET amount through the observation of the change in ECL signal intensity (ΔI). Under optimal experimental conditions, the linear range of PET concentration from 1.0 × 10 −11 mol L −1 to 1.0 × 10 −6 mol L −1 , with a detection limit of 3.3 × 10 −12 mol L −1 (3S/N). This method was successfully applied to determine PET in various vegetable samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Hongkun Li,Qianqian Cai,Yangyang Guan,Zhikang Li,Guifen Jie,Hong Zhou
A multimode sensing and imaging platform based on surface modification strategy of Ru(bpy) 3 2+ doped SiO 2 nanoparticles (RuSiO 2 NPs) was constructed for versatile detection of glutathione (GSH). RuSiO 2 NPs as the luminophore models had outstanding fluorescence (FL) and electrochemiluminescence (ECL) signals. Furthermore, its FL signal could be captured by electrophoresis analyzer, and the ECL signal could be imaged with mobile phone to achieve visualization. So, a unique and convenient multi-mode sensing and imaging platform could be developed based on RuSiO 2 NPs. SiO 2 NPs could realize enrichment of Ru(bpy) 3 2+ based on the nanoconfined effect to achieve signal amplification, and its surface was rich with abundant hydroxyl functional groups, which offered a channel for surface modification. Sulfhydryl modification on the RuSiO 2 NPs was realized by using silane coupling agent, and KMnO 4 was used to in situ generate MnO 2 shell on the surface of RuSiO 2 NPs for quenching its ECL, FL, and colorimetric (CL) signals based on resonance energy transfer (RET). Target GSH could reduce MnO 2 shell to realize recovery of all signals, thus achieving the label-free multimode detection of GSH. Therefore, this work developed a multimode sensing and imaging platform without signal labels or layer-by-layer assembly of electrodes, which had the advantages of simple steps, low cost, and high detection accuracy, showing important application potential in the field of bioanalysis.
研究领域:
生物分析
·
化学传感
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2019
·Vol. 32
·期 3
·462-469
·
IF 2.691
Xiaomeng Shan,Tao Pan,Yuting Pan,Wenchang Wang,Xiaohui Chen,Xueling Shan,Zhidong Chen
In this study, a novel electrochemiluminescence (ECL) sensor for highly sensitive and selective detection of Pb(II) was developed based on Ru(bpy) 3 2+ encapsulated UiO66 metal-organic-framework (Ru(bpy) 3 2+ −UiO66 MOF) and −NH 2 group functionalized silica (NH 2 −SiO 2 ). The NH 2 −SiO 2 with large surface area provided an excellent platform for the ECL sensor. As numerous exposed carboxyl groups were present on UiO66 backbone, the Ru(bpy) 3 2+ −UiO66 could be steadily immobilized to NH 2 −SiO 2 by forming amide bonds. Meanwhile, the introduced UiO66 MOF which used for the encapsulation of Ru(bpy) 3 2+ , significantly enhanced the ECL efficiency of the proposed sensor, as it possessed a large specific surface area and porosity for the loading of Ru(bpy) 3 2+ . Moreover, a high quenching effect on ECL intensity was obtained in the presence of Pb(II) in the electrolyte. Under the optimal conditions, the quenched ECL intensity showed a good linear relationship within Pb(II) concentration in the range from 1.0×10 −6 to 1.0×10 2 μM, with a detection limit of 1.0×10 −7 μM ( S / N =3). The proposed sensor for Pb(II) detection was simple in operation, rapid in testing, stable in signal, and showed a good anti-interference ability to some other metal ions. Besides, its application for detecting Pb(II) in a real sample was also investigated here. This work provides a potential platform for metal ions detection in environmental monitoring field.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Pei Zhang,Yao Zhang,Xin Xiong,Yao Lu,Nengqin Jia
A sensitive electrochemiluminescence (ECL) immunosensor was successfully fabricated utilizing Ru(bpy) 3 2+ -doped mesoporous polydopamine (MPDA) for subtle exploration of the diabetes biomarker glycosylated hemoglobin (HbA1c). In this protocol, with the advantages of relatively large specific surface area and good biocompatibility, MPDA nanoparticles are capable of loading more Ru(bpy) 3 2+ to greatly amplify the ECL efficiency during the existence of trinpropylamine (TPA) as common coreactant. The acquired Ru(bpy) 3 2+ @MPDA nanoparticles are used as a beneficial substrate for immobilizing aptamer, thereby specifically recognizing the targeting substance HbA1c as much as possible with high selectivity. It could be noted from the experimental results that the ECL intensity was evidently reduced as the proposed Ru(bpy) 3 2+ @MPDA ECL immunosensor captured HbA1c, which showed the principle of the ECL quenching determination. Under optimal experimental conditions, the prepared Ru(bpy) 3 2+ @MPDA ECL immunosensor exhibited wide linear range from 0.1% to 18.5% with the low detection limit (LOD) of 0.015% (S/N = 3) and the correlation coefficient (R 2 ) of 0.994. Moreover, the Ru(bpy) 3 2+ @MPDA immunosensor displayed high sensitivity and selectivity for detecting HbA1c. Therefore, the as-obtained Ru(bpy) 3 2+ @MPDA ECL immunosensor will open up a new promising platform for highly sensitive bioassay of HbA1c.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 5
·3157-3164
·
IF 4.036
Yi Xiao,Guofang Wang,Haomin Yi,Suhua Chen,Qinyu Wu,Siyi Zhang,Kexin Deng,Simeng Zhang,Zi-Qi Shi,Xiaoping Yang
Ru(bpy)3Cl2/TPrA is a prominent and widely used ECL system in analytical science. However, the co-reactant TPrA restricts the variety of applications because of its toxicity, volatility, and high cost. Here, we use arginine (Arg) as an alternative co-reactant for Ru(bpy)32+ by taking advantage of its low cost, non-toxicity, and biocompatibility. The mechanism of the Ru(bpy)32+/Arg system is that the deprotonated Arg can react with Ru(bpy)32+ to release emission. The similarity between the Ru(bpy)32+/Arg, Ru(bpy)32+/TPrA, and Ru(bpy)32+/DBAE systems demonstrates that Arg can be used as an alternative co-reactant for Ru(bpy)32+ ECL. As a proof of concept, we achieve an excellent performance for acetaminophen (Ace) detection based on the specificity of Arg and Ace, with excellent linearity, low detection limits, and good recoveries. This work is promising to expand the scope of the Ru(bpy)32+/Arg system and move forward their applications in bioassays.
研究领域:
分析化学
·
生物分析
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-11
·
IF 5.3
Tian Li,Li Huiling,Han Pengfei,Ma Guangping,Wang Wenzhuo,Jiang Hanyue,Lu Juan
An electrochemiluminescence (ECL) sensor was constructed based on Ru(bpy) 3 2+ @Au and GO@CdS for the detection of tartrazine (TTZ). In the presence of the co-reactant TPrA, Ru(bpy) 3 2+ and CdS quantum dots (QDs) were used as dual luminophores to synergistically amplify the ECL signal of the sensor. Gold nanoparticles (Au NPs) enhanced the electron transport capability of the system and thereby amplified the ECL intensity. Graphene oxide (GO), as a carrier, has a special wrinkle-like structure that not only adsorbed a substantial amount of CdS QDs but also prevented their agglomeration. Additionally, a more sensitive detection method was established based on the quenching of the ECL signal, which occurred due to the interaction between Ru(bpy) 3 2+ and TTZ. A low detection limit (1.3 × 10 –13 mol·L −1 , S/N = 3) was obtained for TTZ concentrations ranging from 4.0 × 10 –13 to 4.0 × 10 –8 mol·L −1 . Finally, actual measurement results from three beverage samples demonstrated that the prepared sensors possess excellent practical capabilities for TTZ detection. Graphical
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）