Juan Yang,Dongmiao Qin,Na Wang,Yusheng Wu,Kanjun Fang,Biyang Deng
The construction of advanced systems capable of accurately detecting neuron-specific enolase (NSE) is essential for rapidly diagnosing small-cell lung cancer. In this study, an electrochemiluminescence (ECL) resonance energy transfer immunosensor was proposed for the ultra-sensitive detection of NSE. The co-reactants C2O42− and Ru(bpy)32+ were integrated to form a self-enhanced ECL luminophore (Ru-ZnMOF) as the ECL donor. The abundant carboxyl functional groups of Ru-ZnMOF supported antibody 1 via an amidation reaction. Polydopamine-modified zinc dioxide nanoflowers, as ECL acceptors, inhibited Ru-ZnMOF ECL signaling. The linear range of NSE was 10 fg/mL to 100 ng/mL with a detection limit of 3.3 fg/mL (S/N = 3), which is suitably low for determining NSE in real samples.
The environmental hazards posed by bisphenol A (BPA) have become a critical global concern. Consequently, the development of rapid, user-friendly, and highly sensitive methods for BPA detection is essential for pollution control and health risk assessment. In this study, a novel electrochemiluminescent (ECL) sensor was designed using Ru(bpy) 3 2+ -encapsulated Zn-based metal–organic frameworks (Ru-ZnMOF) integrated with gold nanoparticles (Au@Ru-ZnMOF) as efficient ECL probes. Notably, BPA caused a significant quenching effect on the ECL signal of Au@Ru-ZnMOF in the presence of tripropylamine (TPA) as a coreactant. Under optimized experimental conditions, a glassy carbon electrode modified with Au@Ru-ZnMOF (glassy carbon electrode (GCE/Au@Ru-ZnMOF) demonstrated exceptional ECL performance for BPA detection, with a broad linear detection range (1.0 × 10 −9 to 5.0 × 10 −4 mol·L −1 ) and an ultra-low detection limit of 2.5 × 10 −11 mol·L −1 . The proposed sensor was successfully applied for the quantification of BPA in thermal paper samples, achieving recovery rates between 95.8 % and 98.5 %, confirming its high accuracy and reliability.
危险品化学品经营许可证（带存储）