JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 101
·期 8
·3156-3164
·
IF 2.614
Huihui Ke,Tao Bao,Wei Chen
BACKGROUND Rubus chingii Hu is a widely cultivated fruit in China and has declared multiple bioactivities including antioxidative activity. Ethyl carbamate (EC), mostly found in fermented food and alcoholic beverages, is a recognized human carcinogen, and researchers have proposed the correlation between oxidative stress and its toxicity. This study acquired the polysaccharide from R. chingii (RP) and explored its effect on EC-induced cytotoxicity using Caco-2 cells as the cell model. RESULTS Results showed that RP exhibited protection against EC-induced toxicity by repairing redox imbalance as indicative of mitigated mitochondrial membrane potential collapse, attenuated reactive oxygen species overproduction, and impeded glutathione depletion. Moreover, the structural features of RP were characterized and revealed that it was mainly constituted by galacturonic acid and arabinose, with an average molecular weight of 7.039 × 10 5 g mol −1 . CONCLUSION Overall, our results provided a new approach dealing with the toxicity caused by EC from the perspective of oxidative stress and described a new potential healthy value of R. chingii Hu, which could contribute to the development of a promising dietary supplement and functional food. © 2020 Society of Chemical Industry
研究领域:
食品科学
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毒理学
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营养学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Yuanfang Kong,Yulong Hu,Jieming Li,Juntao Cai,Yuanhao Qiu,Chunhong Dong
Rubus chingii Hu has been used as a functional food for a long time. A novel pectin polysaccharide named RCHP-S from R. chingii Hu was structurally identified and explored its anti-inflammatory effect on colitis mice. RCHP-S was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. NMR spectroscopy and methylation analysis showed that RCHP-S was mainly composed of HG-type pectin domains but also contains a small amount of RG-I. The anti-inflammatory tests indicated that the mouse macrophage RAW 264.7 cells pretreated with RCHP-S could show a significant inhibitory effect on the mRNA level of iNOS, IL-1β, IL-6, and TNF-α in vitro . Polysaccharide RCHP-S reduced the enteritis symptoms in dextran sulfate sodium (DSS)-induced colitis mice by inhibiting released inflammatory factors. These results indicated that the R. chingii Hu polysaccharide can be used as food additives for the treatment of intestinal inflammation.
研究领域:
食品科学
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药理学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2025
·
IF 4
Ping Jiang,Yanfei He,Guangpei Xu,Chuanbo Sun
This study aims to evaluate the mechanism of RCH against CCl 4 -induced liver injury in mice. RCH treatment effectively inhibited CCl 4 -induced liver injury and apoptosis in mice, and reduced liver enzyme activities, blood inflammatory cell and serum inflammatory factor levels. Network pharmacology and machine learning predicted that PI3K-Akt pathway and Met proteins play important roles in RCH anti-CCl 4 -induced liver injury. The results of qRT-PCR and Western blot showed that RCH could down-regulate the expression of the receptor proteins Met, Tlr2, and Tlr4, and inhibit the phosphorylation of Pik3ca and Akt1, and prevent the phosphorylation of NF-κB P65, and decrease cleaved Caspase3 expression. These findings suggest that RCH effectively alleviated liver injury in CCl 4 -induced mice by inhibiting the Met/Tlr2/Tlr4-mediated PI3K-Akt signaling pathway.
研究领域:
药理学
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分子生物学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yuhan Cheng,Yuting Wang,Tongji Yuan,Jianhua Xie,Qiang Yu,Yi Chen
This research aims to evaluate the phenolic composition, antioxidant and enzyme inhibition activities of fermented Rubus chingii Hu wine, and explore the correlation between them. TPC (Total Phenolic content) and TFC (Total Flavonoid content) increased rapidly from 0 h to 72 h, followed by a slight decrease in TPC and a significant decrease in TFC. Fermentation could significantly increase the antioxidant activity and α-amylase/α-glucosidase enzyme inhibitory activity of Rubus chingii Hu . A total of 39 polyphenols and organic acids in fermented Rubus chingii Hu were identified by UPLC-ESI-Q-TOF-MS/MS and 11 of them were quantitatively analyzed. After fermentation, the contents of all the detected polyphenol compounds, except for quercetin and ellagic acid, significantly increased (p < 0.05). Correlation analysis showed that protocatechuic acid and catechin played an important role in the antioxidant activity of fermented Rubus chingii Hu , while protocatechuic acid and hypericin played an important role in the α-amylase inhibition activity. This study indicated that Rubus chingii Hu could be applided as a potential meterial for the wine production, and has the potential to be a functional food for promoting health.
研究领域:
食品科学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2021
·
IF 4.451
Xiaoyan Su,Ming Zhao,Xinyu Fu,Xiaodan Ma,Wei Xu,Songhua Hu
Polysaccharides from Rubus chingii Hu fruits (RFPs) were extracted, purified, characterized and investigated for immunomodulatory activity. Results indicated that RFPs were heteropolysaccharides with molecular weight of 51.7 kDa and contained carbohydrate contents of 96.83%. RFPs with characteristic absorptive peaks of polysaccharide structure and α-configuration were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with a molecular ratio of 0.08:0.17:0.16:0.48:5.73:1.00:1.79:0.03. RFPs promoted splenocyte proliferation, NO release, cytokine production and intracellular Ca 2+ concentration. The mechanism underlying splenocyte activation by RFPs was elucidated by RNA-Seq, in which 2214 differentially expressed genes (DEGs), 2030 GO terms and 92 KEGG pathways were identified. RFPs were further proved to induce a balanced Th1/Th2/Th17 response pattern through MAPK, NF-κB, Jak-STAT and Calcium signaling pathways in splenocytes. These findings provided a fundamental basis for the further application of RFPs as immunomodulators in food and medicine.
研究领域:
免疫学
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药理学
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分子生物学
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生物化学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 16
·2300264
·
IF 3.1
Yu-Jiao Hua,Fen Xie,Kun-jun Mao,Yi-Yuan Luo,Yong-Juan Ding
The fruits of Rubus chingii Hu have high medicinal and nutritional values. However, the metabolite profiles of R. chingii , especially the alterations during different development stages of fruit, have not been comprehensively analyzed, hindering the effective utilization of the unique species. In this study, we comprehensively analyzed the metabolites of R. chingii fruit at four developmental stages using systematic untargeted and targeted liquid chromatography-mass spectrometry metabolomics analysis and identified 682 metabolites. Significant changes were observed in metabolite accumulation and composition in fruits during the different developmental stages. The contents of the index components, kaempferol-3-O-rutinoside and ellagic acid, were the highest in immature fruit. The analysis identified 64 differentially expressed flavonoids and 39 differentially expressed phenolic acids; the accumulation of most of these differentially expressed metabolites decreased with the developmental stages of fruit from immaturity to maturity. These results confirmed that the developmental stages of fruit are a critical factor in determining its secondary metabolite compositions. This study elucidated the metabolic profile of R. chingii fruit at different stages of development to understand the dynamic changes in metabolites.
研究领域:
植物学
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代谢组学
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 2
·214
·
IF 5.1
Mengge Duan,Yuting Wang,Shiyu Chen,Jiawen Lu,Ruihong Dong,Qiang Yu,Jianhua Xie,Yi Chen
Bisphenol A (BPA) and diisobutyl (DIBP) phthalate are widely used as typical plasticizers in food packaging. Plasticizers can be released from polymers, migrate into food, and be ingested by humans, leading to various health problems. However, little research has investigated the combined toxicity of BPA and DIBP, particularly their intestinal toxicity. Our goal is to analyse the combined toxicity of BPA (50 mg/kg) and DIBP (500 mg/kg) on the intestines of KM mice. Additionally, we tried to find natural products that can inhibit or prevent the combined toxicity of BPA and DIBP. The results indicated that the combination of BPA and DIBP exposure resulted in a reduction of beneficial flora, an increase in D-Lac levels (136 ± 14 μmol/L), an increase in intestinal permeability, activation of the notch pathway, and a decline in intestinal stem cells (ISCs) to goblet cells, compared to single-exposure sources. Nevertheless,Rubus chingiiHu phenolic extract (RHPE) (200, 400 and 600 mg/kg) ameliorated the BPA and DIBP-induced intestinal microbiota disruption and intestinal mucosal barrier impairment by inhibiting the overactivation of the notch pathway. The results of this study highlight the potential risks to human health posed by the combination of BPA and DIBP and may help explain the potential pathways of enterotoxicity caused by combined ingestion.
研究领域:
毒理学
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胃肠病学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2026
·Vol. 23
·期 9
·e71696
·
IF 2.9
Cong Zhang,Zepeng Zhang,Fang Yan,Lei Zhang
Tripterygium Glycoside Tablets (TGTs) are primary drugs for rheumatoid arthritis (RA) but pose a risk of nephrotoxicity. Rubus chingii Hu (RCH) may enhance kidney function. This study explores a combined RA treatment by examining RCH's chemical components and using a collagen‐induced arthritis (CIA) rat model to test TGTs with or without RCH. RCH boosted the therapeutic impact of TGTs in CIA rats, shown by decreased paw swelling, improved joint condition, and reduced serum TNF‐α, IL‐6, and IL‐1β levels. The normalization of renal markers (BUN and Scr) and oxidative stress indicators (ROS, T‐NOS, and T‐SOD) may be associated with the Nrf2/NF‐κB pathway. Metabolomics indicated that RCH may counteract nephrotoxicity by influencing the metabolism of glycine, serine, threonine, tryptophan, histidine, taurine, and sulfaurine. In summary, RCH synergizes with TGTs to treat RA by inhibiting inflammation, affecting metabolic pathways, reducing oxidative stress, and minimizing kidney toxicity, offering a new treatment strategy for RA. Experimental flow chart of the attenuating and synergistic effects of RCH on TGTs in the treatment of rheumatoid arthritis.
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2018
·Vol. 13
·期 1
·51-60
·
IF 1.181
Li Kuangyu,Zeng Maolin,Li Qiaoling,Zhou Benhong
Rubus chingii Hu, namely “Fu-pen-zi” in Chinese, is commonly used as a functional food in China. A rapid and sensitive High performance liquid chromatography (HPLC) coupled with linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrap MS) was employed to identify the polyphenolic compounds in R. chingii fruits. A total of 18 compounds were identified in R. chingii polyphenols (RCPs), including five phenolic acids, four ellagitannins, and nine flavonoids. Additionally, the total phenolics, total flavonoids and total tannins content as well as antioxidant and antiproliferative activity were evaluated for the first time. The contents of total phenolics, total flavonoids, and total tannins were 356.42 mg GAE g −1 of extract, 201.94 mg RUE g −1 of extract and 155.12 mg GAE g −1 of extract, respectively. Antioxidant assays showed RCPs had favorable DPPH and OH − radical scavenging capacity and strong ferric ion reducing power. Moreover, the results also indicated the RCPs could inhibit cell proliferation and induce the cell apoptosis in T24 bladder cancer cells. Altogether, the results suggest R. chingii possesses potent antioxidant properties and could inhibit the growth of T24 cells by inducing the cell apoptosis in a dose-dependent manner.
研究领域:
药理学
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肿瘤学
·
分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Dong-Jun Hou,Quan-yuan Xie,Ming-Shun Chen,Yu-Lin Cheng,Bing Zhang,Zong-cai Tu,Lu Zhang
Ellagitannins (ETs) exhibit beneficial effects on gut microbiota modulation and bioactivity, but their instability compromises the delivery efficiency and absorption. Therefore, a double emulsion gel (DEG@RETs) loading with Rubus chingii Hu ETs (RETs) was constructed and optimized to enhance its gastrointestinal digestion stability and intestinal transformation. The optimal DEG@RETs formulation was 4 % pectin, calcium-induced gelation, W 1 /O: W 2 of 4: 6, and W 1 : O of 5: 5. Which achieved an encapsulation efficiency (EE) of 88.68 ± 4.36 %, maintained spherical structures with an EE (78.57 ± 1.34 %) after digestion. The DEG@RETs demonstrated pH and freeze-thaw stability while EE declined after heating at 90 °C. Cryo-SEM confirmed a crosslinked gel network. In vitro fermentation, transformation efficiency demonstrated significantly enhanced with urolithin A, urolithin B and ellagic acid increased by 7.37-, 3.19- and 7.87-fold respectively. These results proved that DEG@RETs can be an effective strategy to improve the colon-targeted delivery and biotransformation efficiency of RETs.
研究领域:
食品科学
·
药剂学
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分子营养学
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生物利用度
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肠道微生物代谢
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）