Marine Drugs
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2022
·Vol. 20
·期 11
·719
·
IF 6.085
Qian Wang,Fu-Jun Liu,Xin-Miao Wang,Guan-Hua Zhao,Dong Cai,Jing-Han Yu,Fa-Wen Yin,Da-Yong Zhou
Low molecular weight (<5 kDa) peptides from mussels (Mytilus edulis) (MPs) and the peptides from clams (Ruditapes philippinarum) (CPs) were prepared through enzymatic hydrolysis by proteases (dispase, pepsin, trypsin, alcalase and papain). Both the MPs and the CPs showed excellent in vitro scavenging ability of free radicals including OH, DPPH and ABTS in the concentration range of 0.625–10.000 mg/mL. By contrast, the MPs hydrolyzed by alcalase (MPs-A) and the CPs hydrolyzed by dispase (CPs-D) had the highest antioxidant activities. Furthermore, MPs-A and CPs-D exhibited protective capabilities against oxidative damage induced by H2O2in HepG2 cells in the concentration range of 25–800 μg/mL. Meanwhile, compared with the corresponding indicators of the negative control (alcohol-fed) mice, lower contents of hepatic MDA and serums ALT and AST, as well as higher activities of hepatic SOD and GSH-PX were observed in experiment mice treated with MPs-A and CPs-D. The present results clearly indicated thatMytilus edulisandRuditapes philippinarumare good sources of hepatoprotective peptides.Keywords:Mytilus edulis;Ruditapes philippinarum;hepatoprotective peptide;H2O2-induced oxidative damage;mice;acute liver injury
研究领域:
生物化学
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药理学
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分子生物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 12
·6421-6431
·
IF 3.3
Jinyu Li,Yunjiao Ma,Feng Xiao,Baoshang Fu,Xianbing Xu
Summary Thermal reactions affect flavour, but how different thermal reactions influence the flavour of cooked soup has rarely been investigated. Herein, the effect of heat treatment (0–50 min) at different temperatures (120, 140 and 160 °C) on Ruditapes philippinarum soup was studied. The acceptable heating of Ruditapes philippinarum soup was assigned at 140 °C for 30 min by human sensory evaluation and compound analysis. In addition, the detailed relationship between the formation of key volatile compounds and heating temperature was revealed by partial least squares discriminant analysis (PLS-DA) and the odour coding method. A total of 28 key volatile compounds were selected according to the results of variables important in the projection (VIP) and odour activity value (OAV). Whereas 3-ethyl-2,5-dimethyl pyrazine and 2-heptylfuran were the most important volatiles produced only in the thermal reaction at 140 °C and associated with cocoa and roasted nut odours. These results indicated the relationship between thermal reactions and flavour formation. The odour coding obtained from OAV could be as a reference benchmark for heating seafood soup products.
研究领域:
食品科学
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化学
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zheng Zhou,Yu-Ying Zhang,Jing-Xuan Gao,Li-Xin Ma,Xu-Hui Huang,Jie Zheng,Xiu-Ping Dong,Lei Qin
This study investigated the changes in free amino acids (FAAs), 5′-nucleotides, and lipid compositions of Manila clams ( Ruditapes philippinarum ) collected from December 2017 to November 2018. High-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was used to quantify twenty-one FAAs and six 5ʹ-nucleotides. Lipid profiles were explored by ultra-performance liquid chromatography coupled with a Q-Exactive Orbitrap mass spectrometer with an untargeted lipidomic approach. Results showed significant monthly changes of FAAs, 5′-nucleotides, and lipids profiles. Alanine , glutamic acid , glycine, and aspartic acid , which contributed to the sweet and umami flavor, were the most abundant FAAs in Manila clams. Totally 90 glycerides, 76 glycerophospholipids , 21 lyso-glycerophospholipids, and 52 fatty acids were identified in Manila clams. Among these lipids, most of them possessed a high-level of unsaturation degree. The unsaturation degree of lipids in February, March, and April was the highest, showing the best nutritional value of Manila clams.
研究领域:
海洋生物学
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代谢组学
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食品科学
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营养学
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高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Lu Wang,Jingjing Miao,Min Ding,Wei Zhang,Luqing Pan
Nonylphenol (NP) contamination in the coastal environment of China poses ecological risks to aquatic organisms. However, the endocrine disruptive impacts of NP on bivalves, particularly on ovarian development, remain poorly understood. In this study, Manila clams Ruditapes philippinarum at the developing stage of gonad were exposed to 1.0 μg/L NP for 21 days. Utilizing RNA interference (RNAi) to suppress ER gene expression, we observed a delay in ovarian development as evidenced by histological observations under both NP and NPRi (NP with ER-RNAi) treatment, with Vtg elevation exclusive to the NP group. Comprehensive analyses encompassing transcriptomics, real-time quantitative PCR, and steroid hormone measurement revealed significant alterations in aldosterone synthesis, estrogen signaling, and thyroid hormone synthesis. These pathways showed similar perturbations in both NP and NPRi groups compared to controls. Notably, the NPRi group exhibited distinct enrichment in PPAR and insulin signaling pathways, may implicating these in ER function suppression. Steroid hormone biosynthesis was notably reduced in both treatments, pointing to a profound impact on hormone synthesis. The contrast between in vivo and in vitro findings suggests that NP's detrimental effects on ovarian development may primarily involve neuroendocrine regulation of steroidogenesis. This investigation highlights the complex dynamics of NP-induced endocrine disruption in bivalves, emphasizing the pivotal role of ER and associated pathways.
研究领域:
环境毒理学
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分子生物学
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内分泌学
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海洋生物学
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PubMed
高影响因子
Marine Drugs
·
2026
·Vol. 24
·期 8
·278
·
IF 5.7
Meng-Yue Liu,Dong-Li Yin,Jia Yu,Wei-Xia Wang,Sheng-Can Zou,Shuang Zhao,Chun-Ze Zou,Fei Li,Yu-Xi Wei
Oral administration represents the primary route for administering polysaccharides. Pharmacokinetics serves as a critical bridge between the administration of polysaccharides and the manifestation of their bioactivities. However, the pharmacokinetic behavior of polysaccharides remains poorly understood. This study aimed to investigate thein vivopharmacokinetics of an orally administeredRuditapes philippinarumpolysaccharide (ERPP), including its plasma concentration–time profile, tissue distribution, and excretion. Moreover, a sensitive quantitative method was developed using 5-DTAF as a fluorescent probe to quantify ERPP levels in rat plasma and tissues. The results revealed that ERPP was absorbed in the gut (Tmax= 3 h), reaching a peak concentration (Cmax) of 22.7 ± 2.7 mg/L. Subsequently, it predominantly accumulated in the kidneys, liver, and lungs. Complementarily, near-infrared fluorescence (NIR) imaging provided real-time qualitative visualization of ERPP-Cy5.5 tissue distribution, corroborating the organ-level accumulation pattern observed in the quantitative study. Excretion studies over a 24 h observation window indicated that ERPP was primarily excreted via feces, with 45.7 ± 4.4% of the recovered dose within this period; the complete excretion profile warrants further investigation at extended time points. These findings provide pharmacokinetic insights into thein vivofate of marine polysaccharides and support further biological evaluation.
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JOURNAL OF FOOD ENGINEERING
·
2025
·
IF 5.8
Meihuan Li,Ning Cong,Jiateng Chen,Ling Zhang,Xuanpeng Wang,Ming Du,Xianbing Xu,Jinfeng Pan,Qiyue Zhao
Ruditapes philippinarum produces turbidity during hydrolysis, which may seriously hinder its further processing and utilization as a flavoring agent. In this study, the low-temperature crystallization properties of the oil components in the mixing system were combined with freezing flocculation to promote the aggregation, growth, and settling of the oil-peptide mixtures by ice crystal extrusion, which effectively solved the problems of secondary contamination and high cost associated with the traditional flocculation methods, while retaining the flavor of the enzymatic hydrolysate. The freezing process increased the contents of fresh, sweet, essential and total free amino acids in the supernatant by 1.94 %, 1.69 %, 1.45 % and 0.58 %, respectively, and these flavor amino acids optimized the flavor profile of the enzymatic digest. Freezing flocculation is a clean and sustainable flocculation technology that supports the sustainable development of the food industry and is in line with the trend of green production and environmental protection.
研究领域:
食品科学
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生物技术
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环境科学
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海洋生物学
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Yijing Han,Qianqian Zhang,Lizhu Chen,Jianmin Zhao,Dinglong Yang
Deltamethrin (DLM), a broad-spectrum pesticide, has been proven to have toxic effects on aquatic organisms. Here, we detected the formation of extracellular traps (ETosis) formation in Manila clam ( Ruditapes philippinarum ) hemocytes stimulated by three concentrations of DLM (0.01, 0.1 and 1 μg/mL) in vitro, and explored the underlying mechanisms induced by this pesticide. Extracellular DNA structure observation and quantitative results indicated that DLM exposure could obviously induce hemocytes ETosis, especially under high concentration of DLM induction. Moreover, DLM increased the levels of myeloperoxidase (MPO) and reactive oxygen species (ROS) in a dose-dependent manner, and enhanced the mRNA expression of several ROS-related genes. DPI (NADPH oxidase inhibitor) and ABAH (MPO inhibitor) could substantially inhibit DLM-induced extracellular traps (ETs), suggesting that the induced ETs release was caused by the induction of the ROS burst and MPO production. In addition, three concentrations of DLM-induced ETs were also accompanied by mitochondrial dysfunction, such as increasing the production of mitochondrial ROS, leading to a decrease in mitochondrial membrane potential (MMP) and activation of mitochondrial permeability transition pore (MPTP). Taken together, these results will shed new light on the immunotoxicity of DLM in clams and perhaps lays the foundation for health assessment in bivalves.
研究领域:
水生毒理学
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免疫毒理学
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海洋生物学
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环境科学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Shiyuan Wang,Yaokuan Wang,Fangchen Li,Qiao Jin,Wei Li,Tanye Xu,Min Qu,Changqing Tong
DSEDLPL was the peptide with better Ca 2+ -binding activity via molecular docking. • RPP-Ca had good stability (heat/digestive resistance; Ca stability in neutral/weakly alkaline). • RPP-Ca had high safety. • RPP-Ca had good bioavailability compared with calcium ions. This study optimized the preparation of peptide‑calcium chelates from Ruditapes philippinarum protein (RPP). Under optimal conditions, RPP's calcium-binding capacity reached 95.79 ± 2.09 mg/g. Structural characterization (FTIR, UV, SEM) and molecular docking revealed that chelation occurred via coordination bonds, hydrophobic interactions, and hydrogen bonds (binding energy: −5.74 kcal/mol, primarily involving carboxyl oxygen atoms in a bidentate pattern. RPP-Ca demonstrated high stability, maintaining an 80%–85% calcium retention rate at pH 6.0–10.0. Furthermore, calcium transport assays showed that RPP-Ca’s transport capacity was 42.20 ± 1.54% higher than that of inorganic CaCl 2 . These findings indicate that RPP-Ca is a stable, high-affinity chelate with superior bioavailability, making it a promising candidate for functional calcium supplementation.
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PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 23
·4583
·
IF 4.6
Ting Zhang,Jiale Song,Zhenzhen Peng,Mengjiao Wu,Zhi Li,Fei Li,Yuxi Wei
Polysaccharides from marine organisms have been extensively studied and utilized as functional food ingredients due to their excellent immunomodulatory properties. However, the immunomodulatory potential of fermentedRuditapes philippinarumpolysaccharide (RPP) has not been systematically explored. This study investigated the effects of RPP on immune function in cyclophosphamide (CTX)-induced immunosuppressedBALB/cmice. These results revealed that RPP alleviated CTX-induced weight loss and restored appetite. Moreover, RPP can promote the morphology and indices of immune organs, as well as increased the number of white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb). Serum levels of interleukin-6 (IL-6) and immunoglobulin E (IgE) were significantly elevated following RPP treatment. Additionally, RPP improved colonic morphology by upregulating the expression of E-cadherin and ZO-1 and promoting the secretion of secretory IgA (sIgA). These results indicated that RPP exerted an immune protective effect inBALB/cmice and justified its further potential as a bioactive ingredient for functional foods derived from marine shellfish.
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DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
·
2021
·
IF 3.636
Yijing Han,Qianqian Zhang,Lizhu Chen,Dinglong Yang,Jianmin Zhao
The formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum , and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes ( PI3K , AKT and HIF ). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum , Vibrio harveyi , Escherichia coli and Micrococcus luteus . Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosan-induced ETs.
研究领域:
免疫学
·
海洋生物学
·
细胞生物学
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PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2025
·
IF 3.9
Yang Liu,Kai Liao,Yuxiang Zhu,Yang Zhang,Hailong Huang,Hui Ge,Jilin Xu
Taurine serves an important role in all aquatic animals. The purpose of this experiment was to investigate the effects of exogenous taurine on growth, endogenous taurine metabolism, and Vibrio crassostreae resistance in juvenile clam Ruditapes philippinarum . Microencapsulated taurine concentrations of 0, 0.75, 1.5, 3 and 6 mg/g clam wet weight were designated as the T0, T0.75, T1.5, T3 and T6 groups. After 2 weeks of feeding, the results demonstrated that exogenous taurine did not promote the growth of clams ( P > 0.05). The taurine content of clams was increased significantly with the increase of exogenous taurine content ( P < 0.05). The gene expression of taurine transporter ( TauT ) was significantly elevated in the T3 and T6 groups compared to the T0 group ( P < 0.05). With the addition of exogenous taurine, mRNA expressions of cysteine sulfinic acid decarboxylase ( CSAD ) , 2-aminoethylsulfide dioxygenase ( ADO ) , cysteine dioxygenase ( CDO ) , cystathionine β-synthase ( CBS ) , cystathionine γ-lyase ( CSE ) and serine dehydratase ( SDH ) were suppressed ( P < 0.05). The survival rate of clams challenged with Vibrio crassostreae was significantly higher in the T0.75 group compared to the T0 group on the fifth day ( P < 0.05). Additionally, the activities of antioxidant enzymes and lysozyme were significantly elevated, immune-related and anti-inflammatory factor genes were upregulated, while pro-inflammatory factor genes were downregulated in the T0.75 group compared to the T0 group ( P < 0.05). Dietary taurine supplementation regulates endogenous taurine metabolism and exhibits potential anti- Vibrio effect in clam R. philippinarum .
研究领域:
水产养殖学
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分子生物学
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免疫学
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PubMed
高影响因子
Frontiers in Marine Science
·
2021
·
IF 4.912
Han Yijing,Hu Gege,Chen Yuying,Chen Lizhu,Yu Daode,Zhang Qianqian,Yang Dinglong
Although defensins have been isolated from a variety of metazoan, their role in cellular immunity has not been answered. In the study, we found that the hemocytes of the Manila clams Ruditapes philippinarum release defensin (designated as Rpdef3) in response to Vibrio parahaemolyticus challenge. The antimicrobial Rpdef3 was proved to be involved in the extracellular traps (ETs) that hemocytes released in response to Vibrio challenge. Scanning electron microscopy observation proved the patterns how ETs eliminate invading bacteria. Furthermore, Rpdef3 involved in ETs had broad-spectrum antimicrobial effect on both Gram-negative bacteria and Gram-positive bacteria. ELISA assay revealed that Rpdef3 could bind lipopolysaccharides and peptidoglycan in a dose-dependent manner. As concerned to the antibacterial mechanisms, Rpdef3 can cause bacterial membrane permeabilization, leading to cell death. As a result, Rpdef3 might contribute to the trap and the elimination of invading Vibrio in clam ETs. Taken together, our study suggest that the formation of ETs is a defense mechanism triggered by bacterial stimulation, coupled with antibacterial defensin.
研究领域:
免疫学
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海洋生物学
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微生物学
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高影响因子
危险品化学品经营许可证（带存储）