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Discovery and application of methionine sulfoxide reductase B for preparation of (S)-sulfoxides through kinetic resolution

Yuanmei Wen,Liaotian Peng,Yang Zhou,Tao Peng,Yu Chen,Xiaoling Cheng,Yongzheng Chen,Jiawei Yang

Here we report a methionine sulfoxide reductase B (MsrB) enzymatic system for the preparation of ( S )-sulfoxides through kinetic resolution of racemic ( rac ) sulfoxides. Eight MsrB homologue recombinant proteins were expressed and their activities on asymmetric reduction of rac -sulfoxides were analyzed. Among these MsrB homologue proteins, one protein from Acidovorax species showed good activity and enantioselectivity towards several aryl-alkyl sulfoxides. The ( S )-sulfoxides were prepared with 93–98% enantiomeric excess through kinetic resolution at initial substrate concentration up to 50 mM. The establishment of MsrB catalytic kinetic resolution system provides a new efficient green strategy for preparation of ( S )-sulfoxides.