JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
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2018
·Vol. 88
·期 3
·654-659
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IF 1.745
Wang Lijuan,Lv Hua,Li Binjie,Zhao Yanbao,Sun Lei
Flower-like ceria (CeO 2 ) architectures consisting of well aligned nanosheets were first synthesized by a glycol solvothermal method. The size of CeO 2 architectures is about 5 μm in width and 10 μm in length, with the nanosheets thickness below 100 nm. Subsequently, the adsorbed Ag ions on the surface of CeO 2 were in situ reduced to form Ag nanoparticles (NPs), leading to the fabrication of Ag/CeO 2 hybrid architectures (HAs). The formed Ag NPs with sizes of 20–40 nm were uniformly loaded on the surface of the CeO 2 sheets. The antibacterial properties of Ag/CeO 2 HAs against Gram-negative E. coli and Gram-positive S. aureus were evaluated by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and a filter paper inhibition zone method. The results demonstrated that Ag/CeO 2 HAs displayed excellent antibacterial activity toward S. aureus and E. coli , which were attributed to the synergistic antibacterial effect between Ag NPs and CeO 2 in HAs . Here, CeO 2 nanoflowers as a new substrate could restrict Ag NPs aggregations and improve their antibacterial activities. Therefore, the resulted Ag/CeO 2 HAs would be considered as a promising antibacterial agent.
研究领域:
材料科学
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微生物学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Lingyan Yang,Liliang Chen,Yi-Chun Chen,Lei Kang,Jiantao Yu,Yuhang Wang,Chengjia Lu,Tsutomu Mashimo,Akira Yoshiasa,Chia-Hua Lin
Immiscible bimetallic Ag–Cu system has been synthesized by the pulsed plasma in liquid method with a graph of one pulse duration. Herein, by combining X-ray power diffraction, K-edge X-ray absorption near edge structure and high-resolution transmission electron microscopy, our results indicate that homogeneously alloyed Ag–Cu nanoparticles (Ag–Cu NAs) have the average diameter about 2.1 nm, composed by 48.5 at% Ag and 51.5 at% Cu with chemical analysis and the estimated lattice parameter was 3.873 Å. The antibacterial property of Ag–Cu NAs was detected against E. coli and S. aureus strains according to the colony formed abilities of bacteria on agar plates covered with the nanoparticles. With very short incubation period, Ag–Cu NAs completely inhibited the E. coli and S. aureus growth at an ultralow concentration. The mechanism of antibacterial property of Ag–Cu NAs was performed by the inductively coupled plasma-atomic emission spectrometry and the plane wave pseudopotential method implemented in the CASTEP package based on the density functional theory. The Ag + dissolution is correlated with antibacterial activity for Ag–Cu NAs-assisted antibacterial treatment. These findings obtained revealed that our Ag–Cu NAs could be served as a containing material of numerous bacteria-free products in order to avoid their bacterial contamination.
研究领域:
材料科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2016
·Vol. 40
·期 7
·6332-6339
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IF 3.277
Ren Liu,Huiwen Ge,Xue Wang,Jing Luo,Zhiquan Li,Xiaoya Liu
In this paper, a facile, economical and environmentally friendly synthetic method was developed to fabricate a Ag nanoparticle (NP) decorated graphene (GR/TA/Ag) composite with tannic acid (TA) as a reducing agent and stabilizing agent. The GR/TA/Ag composite was fully characterized using Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible absorption spectroscopy (UV-vis), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) demonstrated that Ag NPs with diameters of up to 6 nm were homogeneously and uniformly deposited on graphene sheets. With an increasing concentration of AgNO3 used for immersion, the amount of Ag NPs loaded kept increasing without aggregation or deformation. The antibacterial properties of the GR/TA/Ag composite were studied using Gram-negative E. coli ATCC 25922 and Gram-positive S. aureus ATCC 6538 via both the disk diffusion method and the shaking flask method. The disk diffusion method results showed that the synthesized GR/TA/Ag composite had great release antibacterial properties owing to the loading of Ag NPs. In addition, the presence of TA gave the composite good non-release antibacterial properties, based on the results of the shaking flask method. The ingenious combination of the release-killing capabilities of Ag NPs and the contact-killing capabilities of TA bestow two-level antibacterial activity on the GR/TA/Ag composite, promoting the GR/TA/Ag composite as part of a new generation of powerful antibacterial agents with promising applications.
研究领域:
材料科学
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微生物学
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DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2015
·Vol. 31
·期 1
·160-166
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IF 0.875
Zuo Huajiang,Wen Wanhua,Wu Dingcai,Fu Ruowen,Tang Chunyi
Copolymers of quaternary ammonium monomer(QAM) and hydrophilic co-monomer were successfully synthesized by free radical polymerization. It was discovered that the hydrophilic co-monomers with poor antibacterial activity significantly enhanced the activity of QAM substituted with a long alkyl chain[ i.e. , N , N -dimethyl- N -dodecyl methacrylate ammonium bromide(DMAEMA-DB)]. When a suitable molar ratio of DMAEMA-DB to co-monomer was selected, the activity of the copolymers was up to 123 times that of the homopolymer of DMAEMA-DB against S. aureus , and 282 times that of it against E. coli . But unlike DMAEMA-DB, the co-monomers might weaken the activity of QAM substituted with a short alkyl chain[ i.e. , N , N -dimethyl- N -butyl methacrylate ammonium bromide(DMAEMA-BB)]. Moreover, it was found that copolymers of DMAEMA-DB were much more biocidal than those of DMAEMA-BB. Therefore, it could be speculated that the long alkyl chain plays an important role in the antibacterial activity, and that the hydrophilic co-monomers are beneficial to polymeric guaternary ammonium compounds(PQACs) to exert the positive effect of the long alkyl chain to the greatest degree.
研究领域:
高分子化学
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微生物学
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2023
·
IF 5.4
Yufang Li,Xiaozhao Tang,Zushun Yang,Jinze He,Ni Ma,Aixiang Huang,Yanan Shi
The combination of natural antimicrobial peptide BCp12/phenyllatic acid (BCp12/PLA) presents a more efficient antibacterial effect, but its antibacterial mechanism remains unclear. This study studied the synergistic antibacterial mechanism of BCp12 and PLA against S. aureus . The results demonstrated that the BCp12/PLA combination presented a synergistic antibacterial effect against S. aureus , with a fractional inhibitory concentration of 0.05. Furthermore, flow cytometry and scanning electron microscope analysis revealed that BCp12 and PLA synergistically promoted cell membrane disruption compared with the group treated only with one compound, inducing structural cell damage and cytoplasmic leakage. In addition, fluorescence spectroscopy analysis suggested that BCp12 and PLA synergistically influenced genomic DNA. BCp12 and PLA targeted enzymes related to peptidoglycan and DNA synthesis and interacted by hydrogen bonding and hydrophobic interactions with mur enzymes (murC, murD, murE, murF, and murG), dihydrofolate reductase, and DNA gyrase. Additionally, the combined treatment successfully inhibited microbial reproduction in the storage of pasteurized milk, indicating that the combination of BCp12 and PLA can be used as a new preservative strategy in food systems. Overall, this study could provide potential strategies for preventing and controlling foodborne pathogens.
研究领域:
微生物学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
·
IF 5.999
Xiaomei Dai,Xiaojun Liu,Lele Yang,Siyuan Yuan,Qingqing Xu,Yu Li,Feng Gao
Intracellular bacterial infections pose enormous challenges to food safety and public health. Antibiotic-based polymer prodrugs have been used to treat intracellular bacterial infection. However, the overuse of antibiotics may lead to the emergence of antibiotic resistance . In this work, we aimed to develop antibiotic-free pH-responsive polymeric prodrugs to combat intracellular S. aureus infection. Amphiphilic poly(ethylene glycol)- b -poly[(3-phenylprop-2-ene-1,1-diyl)bis(oxy)bis(enthane-2,1- diyl)diacrylate] (PEG- b -PCAE) was obtained by radical polymerization and they could self-assemble to form micelles . PEG- b -PCAE micelles could uptake by macrophage. Upon exposure to the acidic phagolysosome, PEG- b -PCAE micelles could release cinnamaldehyde (CA) through hydrolysis of the acetal linkage. PEG- b -PCAE could kill intracellular bacteria by damaging the bacterial membrane. Furthermore, PEG- b -PCAE micelles could generate reactive oxygen species (ROS) in macrophages and subsequently activate immune system to clear bacteria by inducing macrophages differentiation to M1 phenotype. PEG- b -PCAE micelles could accelerate the wound healing process of the S. aureus -infected model in vivo . It is anticipated that multifunctional antibiotic-free PEG- b -PCAE micelles with intrinsic antibacterial activities hold promise for improved outcomes in intracellular S. aureus infections.
研究领域:
微生物学
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免疫学
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药物化学
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DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2022
·
IF 6.064
Zheng Laibao,Shen Yunqiu,Dong Wenjia,Zheng Chaochuan,Zhou Ruolan,Lou Yong-Liang
Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for public health, especially in resource-limiting regions. Herein, we developed a rapid, portable, and universal detection method for bacteria using AgNPs-invertase complexes and the personal glucose meter (PGM). In the presence of bacteria, the invertase could be released from AgNPs-invertase complexes where its enzyme activity of invertase was inhibited. Then, the enzyme activity of invertase was restored and could convert sucrose into glucose measured by a commercially PGM. There was a good linear relationship between PGM signal and concentration of E. coli or S. aureus as the bacteria model with high sensitivity. And our proposed biosensor was proved to be a rapid and reliable method for antimicrobial susceptibility testing within 4 h with consistent results of Minimum Inhibitory Concentrations (MICs) testing, providing a portable and convenient method to treat infected patients with correct antibiotics and reduce the production of antibiotic-resistant bacteria, especially for resource-limiting settings.
研究领域:
微生物学
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医学诊断
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生物传感器技术
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2021
·
IF 5.097
Leilei Sun,Sisi Shi,Hongchao Geng,Yao Huang,Yan Qiao,Jie Song,Lan Yang,Craig A. Grimes,Xinxin Feng,Qingyun Cai
Bacterial infections are a serious ongoing threat to human health. As such, within the context of modern technology, there is an urgent need to develop fluorescent probes suitable for diagnosing bacterial infections in vivo. Herein, we synthesize NaGdF4:Nd@NaGdF4 core-shell down-conversion nanoparticles (DCNPs) demonstrating excellent emission intensity in the second near-infrared (NIR-II) window for use as fluorescent probes to enable high-quality imaging of pathogenic infections. The fluorescent probe is covalently linked with vancomycin (Van), which specifically binds to Gram-positive bacteria such as S. aureus. Both in vitro detection and in vivo imaging results show that the Van-modified NIR-II fluorescent probe can distinguish Gram-positive from Gram-negative bacteria with a high degree of selectivity. Biocompatibility is verified by MTT assay and histological analyses.
研究领域:
生物医学工程
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材料科学
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感染性疾病诊断
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Longlong Wang,Sirong Sun,Xingyu Jin,Jiayu Cheng,Heng An,Zhou Yang,Xueji Zhang,Haifeng Dong,Xiangdan Meng
Rapid and reliable detection of Staphylococcus aureus ( S. aureus ), a major foodborne pathogen, remains a critical challenge in food safety surveillance. Here, we present a hybrid electrospinning–CHA–SERS biosensing platform that integrates aptamer-functionalized electrospun nanofibers with catalytic hairpin assembly (CHA)-mediated assembly of gold nanoparticles (GNPs) for ultrasensitive detection of S. aureus . The electrospun nanofibers enable efficient bacterial enrichment through their high-surface-area architecture, while CHA-triggered GNPs aggregation generates dense plasmonic “hotspots” for remarkable Raman enhancement. This synergistic design achieves a record-low detection limit of 9.2 CFU/mL, along with high specificity and stability in complex food matrices. Demonstrated in real samples, this work establishes a new signal amplification paradigm that couples enzyme-free nucleic acid assembly with SERS enhancement, offering a powerful strategy for intelligent food safety monitoring and rapid pathogen diagnostics.
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Que Bai,Fangfang Hu,Siyuan Gou,Qian Gao,Shihao Wang,Wenhui Zhang,Yanni Zhang,Tingli Lu
The damaged skin for some reasons is vulnerable to invasion by bacteria and other harmful microorganisms, leading to delay of the wound healing. In order to promote the infected wound healing, curcumin was loaded with chitosan-based hydrogel was formed through phenylborate ester bonding and its properties and effects on the S. aureus -infected wound healing was tested. It was found the hydrogel showed good antioxidation on the intracellular reactive oxygen species, inhibition on the growth of S. aureus , and acceleration the infected skin healing. The ablity of hydrogel due to its regulating inflammation, promoting angiogenesis and collagen synthesis in the wound site. This research work suggested that the developed multifunctional hydrogel might be a beneficial treatment for the infected wound healing.
研究领域:
药学
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生物医学工程
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伤口愈合
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感染控制
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Mengru Du,Wenbo Zhao,Ruonan Ma,Hangbo Xu,Yupan zhu,Chongxin Shan,Kaikai Liu,Jie Zhuang,Zhen Jiao
Waterborne diseases caused by pathogenic microorganisms pose severe threats to human health. ZnO nanoparticles (NPs) hold great potentials as an effective, economical and eco-friendly method for water disinfection , but the exact antimicrobial mechanism of ZnO NPs under visible-light illumination is still not clear. Herein, we investigate the visible-light-driven photocatalytic inactivation mechanism of amino-functionalized hydrophilic ZnO (AH-ZnO) NPs against Staphylococcus aureus ( S. aureus ) in aqueous environment from the perspective of electron transfer theory. The results show that the antibacterial effects of AH-ZnO NPs are dependent on the AH-ZnO NPs concentration and treatment time. The bulk ORP value and released Zn 2+ concentration in AH-ZnO NPs solutions increase with AH-ZnO NPs concentration. The SEM and intracellular protein leakage results indicate that AH-ZnO NPs can adhere to S. aureus surface without causing obvious cell membrane disruption. The photoluminescence (PL) intensity and fluorescence lifetime of AH-ZnO NPs are remarkedly decreased after adding S. aureus , which confirms the electron transfer from S. aureus to AH-ZnO NPs. Moreover, the ΔPL intensity is closely correlated with the inactivation efficiency, demonstrating that the interfacial electron transfer in S. aureus /AH-ZnO NPs composites contributes to the antibacterial activity, which is speculated to disrupt the normal respiratory electron transfer chain of S. aureus , thereby causing intracellular ROS generation, cell membrane depolarization and eventually apoptosis-like death.
研究领域:
环境科学
·
纳米技术
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微生物学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Lixia Guo,Yafei Tian,Liang Zhou,Shiyue Kang,Chengwu Zhang,Wen Liu,Haipeng Diao,Liheng Feng
Phototherapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), is a promising treatment approach for multidrug resistant infections. PDT/PTT combination therapy can more efficiently eliminate pathogens without drug resistance. The key to improve the efficacy of photochemotherapy is the utilization efficiency of non-radiation energy of phototherapy agents. Herein, a facile phototherapy molecule (SCy-Le) with the enhancement of non-radiative energy transfer is designed by an acid stimulation under a single laser. Introduction of the protonated receptor into SCy-Le results in a distorted intramolecular charge in the infected acidic microenvironment, pH ≈ 5.5, which in turn, enhances light capture, reduces the singlet–triplet transition energies (Δ E S1–T1 ), promotes electron system crossing, enhances capacity of reactive oxygen species generation, and causes a significant increase in temperature by improving vibrational relaxation. SCy-Le shows more than 99% bacterial killing rate against both methicillin-resistant Staphylococcus aureus and its biofilms in vitro and causes bacteria-induced wound healing in mice. This work will provide a new perspective for the design of phototherapy agents, and the emerging photochemotherapy will be a promising approach to combat the problem of antibiotic resistance.
研究领域:
微生物学
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药理学
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感染与免疫
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产品关联
DOI
PubMed
高影响因子
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