PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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2022
·Vol. 24
·期 37
·22710-22715
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IF 3.945
Dongyuan Yang,Yanjun Min,Baihui Feng,Xueming Yang,Guorong Wu
The S1(1ππ*) state decay dynamics of 2-pyridone excited around the 000 band origin is investigated using femtosecond time-resolved photoelectron imaging technique. At a pump wavelength of 334.0 nm, the vibrational ground state and a few low energy vibrational states covered by the bandwidth of the pump laser pulses are excited. The lifetimes of the vibrational states show strong dependence on the vibrational energy and mode. A quantum beat between two lowest energy vibrational states is also observed. This study provides quantitative information about the vibrational-state dependent lifetime of the S1 state of 2-pyridone.
研究领域:
化学动力学
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光谱学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL PHYSICS
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2022
·Vol. 35
·期 2
·242-248
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IF 1.09
Min Yan-jun,Yuan Wen-peng,Yang Dong-yuan,Dai Dong-xu,Yu Sheng-rui,Wu Guo-rong,Yang Xue-ming
The S 1 state decay dynamics of 2-hydroxypyridine following UV excitation at a wavelength range of 276.9−250.0 nm is investigated using femtosecond time-resolved photoelectron imaging technique. Based on pump wavelength dependence of the decay dynamics, a refined decay picture is proposed. At pump wavelength of 276.9 nm, the S 1 state is depopulated through intersystem crossing to lower triplet state(s). At 264.0 nm, both intersystem crossing to lower triplet state(s) and internal conversion to the ground state are in operation. At 250.0 nm, internal conversion to the ground state becomes dominated.
研究领域:
化学动力学
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光物理化学
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2015
·Vol. 20
·期 2
·413-419
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IF 2.446
Zhou Yunlei,Wang Mo,Yang Zhiqing,Lu Linan,Yin Huanshun,Ai Shiyun
Nuclease S1 can catalyze the nonspecific endo- and exonucleolytic cleavage of single-stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. However, it cannot hydrolyze double-stranded DNA, double-stranded RNA, or DNA-RNA hybrid. Inspired by this specific property, a simple electrochemical method was developed for microRNA detection based on hybridization protection against nuclease S1 digestion. In the absence of hybridization process, the assembled probe DNA on the electrode surface can be easily digested by nuclease S1 and a strong electrochemical signal can be generated due to the decreased repulsive force towards the redox probe. However, after hybridization with target microRNA, the digestion activity of nuclease S1 is inhibited, which can lead to a weak electrochemical signal. Based on the change of the electrochemical signal, the detection of target microRNA-319a can be achieved. Under optimal experiment conditions, the electrochemical signal was proportional to microRNA-319a concentration from 1000 to 5 pM and the detection limit was 1.8 pM (S/N = 3). The developed method also showed high detection selectivity and reproducibility. Furthermore, the proposed method was successfully applied to assay the expression level of microRNA-319a in the leaves of rice seedlings after being incubated with different concentrations of 6-benzylaminopurine.
研究领域:
生物化学
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分析化学
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植物生物学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2016
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IF 4.917
Zhiyi Sun,Yongkang Lv,Yuxiang Liu,Ruipeng Ren
A novel heterotrophic nitrifying and metal resistant bacterium was isolated and identified as Cupriavidus sp. S1. The utilization of ammonium, nitrate and nitrite as well as the production of N 2 proved the heterotrophic nitrification and aerobic denitrification ability of S1. The ammonium, nitrate and nitrite removal efficiencies were 99.68%, 98.03% and 99.81%, with removal rates of 10.43, 8.64 and 8.36 mg/L/h, respectively. A multiple regression equation well described the relationship between carbon source utilization, cell growth and nitrification. Keeping the shaking speed at 120 rpm was beneficial for denitrification. Moreover, different forms of nitrogen source could be utilize in simultaneous nitrification and denitrification. Additionally, the efficient removal of ammonium occurred at 20.0 mg/L Zn 2+ , or 10.0 mg/L Ni 2+ or 8.0 mg/L Cu 2+ or 5.0 mg/L Cr 6+ , 33.35 mmol/L sodium pyruvate, C/N 12–28. These findings demonstrate that S1 was effective for nitrogen removal in industrial wastewater containing heavy metal.
研究领域:
微生物学
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环境科学
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废水处理技术
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DOI
PubMed
高影响因子
Analytical Methods
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2018
·Vol. 10
·期 12
·1405-1412
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IF 2.073
Chuan He,Jinli Zhang,Wei Li,Yan Fu
Herein we propose a rational design to construct peroxidase mimetics in response to S1 nuclease by employing guanine-rich oligonucleotides and Cu2+ ions. The enzymatic activities of DNA–Cu(II) are highly dependent upon the binding stoichiometry between Cu2+ and DNA. In the H2O2-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine, the catalytic activity of Cu2+ can be accelerated approximately 88 times in the presence of AG3(T2AG3)3, whereas it shows slight enhancement in the presence of a mononucleotide (GMP). The coordination of Cu2+ with DNA structures greatly strengthens its ability to generate reactive oxygen species. The most intriguing finding in this study is that DNA–Cu(II) complexes lose their enzymatic activities after the cleavage of DNA scaffolds by S1 nuclease. A colorimetric method was established for selectively evaluating the S1 nuclease activity with a limit of detection of 4.7 × 10−3 U mL−1, only by using label-free oligonucleotides and copper ions without complicated synthesis and apparatus. This facile method can also be applicable for quantitative analysis in biological fluids.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
VETERINARY RESEARCH
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2025
·
IF 3.5
Su Kai,Ren Jing,Zhang Yanan,Yuan Chen,Wang Yawen,Yang Liu,Fu Lizhi,Fan Tingli,Song Qinye
Porcine epidemic diarrhea (PED), a highly contagious intestinal disease caused by PEDV, threatens newborn piglets and causes enormous losses in the swine industry. Intestinal mucosal immunity is vital for defense against PEDV. Chitosan (CS), a mucin-adhesive biodegradable polysaccharide, has emerged as a promising mucosal vaccine delivery vector and antigen adjuvant. To develop a PEDV mucosal vaccine, we prepared S1-CS nanoparticles (NPs) loaded with the S1 domain of the PEDV spike (S) protein. We evaluated their ability to induce mucosal immune responses in mice via different inoculation routes and to confer immune protection in piglets after oral inoculation. The results showed that S1-CS NPs exhibited no cytotoxicity and remained stable in simulated gastric fluid in vitro. Compared with oral administration, intramuscular inoculation of S1-CS NPs induced higher levels of PEDV-specific serum IgG in mice. However, PEDV-specific IgA was detected only in the serum and intestinal lavage fluid of orally vaccinated mice. Oral administration also elicited higher IFN-γ levels than intramuscular injection, suggesting stronger activation of cellular immunity. In piglets, oral S1-CS NPs induced serum neutralizing antibodies and IgA responses in both serum and intestinal mucosa, increased the number of IgA-secreting cells in the intestine, reduced viremia and virus shedding, and improved intestinal villus morphology. These findings indicate that the S1-CS NPs developed in this study represent promising candidates for an oral mucosal PEDV vaccine.
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PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2022
·Vol. 98
·期 2
·498-505
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IF 3.709
Qingping Du,Zuanjia Zhong,Ting Zhang,Yanbin Xu,Guoqing Zhang,Shangquan Wu,Jiaxin Xu
Background Biotricking filtration (BTF) based on absorption and biodegradation of waste gas by microorganisms in a biofilm can be used to degrade with difficulty hydrophobic volatile pollutants. Photocatalysis is a promising technology for the abatement of gaseous contaminants because of its nonselective oxidation of volatile organic compounds; however, photocatalysis technology has the limitations of high energy consumption and low mineralization efficiency. This study, therefore, aimed to investigate the synergistic effects of UV/TiO 2 photocatalysis (PCO) pretreatment and BTF in treating gaseous m -xylene. Results BTF incubated with a mold, Aspergillus sp. S1, was acclimated with m -xylene as the sole carbon source. A combined system of PCO with BTF was developed to remove gaseous m -xylene. The results showed that the startup period was shortened by 3 days in the PCO-BTF combined system compared to that with BTF alone. The removal efficiency reached 73% and 91% for the BTF and PCO-BTF systems respectively when the empty bed residence time was extended to 90 s. In addition, PCO-BTF could withstand transient shock loading of m -xylene of 5000 mg m −3 and gradually restore its decontamination performance. Conclusions PCO pretreatment was effective in the enhancement of the removal capacity of BTF for m -xylene. The PCO-BTF system proposed in this study provides an attractive alternative to abate hydrophobic volatile pollutants. The integration of PCO essentially prompted the capability of Aspergillus sp. S1 in the BTF unit, which paves the way for the treatment of gaseous waste at an industrial scale. © 2022 Society of Chemical Industry (SCI).
研究领域:
环境工程
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生物技术
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化学工程
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DOI
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Lei Wang,Chengcheng Feng,Yuxin Chen,Qingqing Meng,Jingwei Li,Yi Liu,Wenqian Zhang,Zhe Li,Jianhua Qu,Ying Zhang
The global concern surrounding pollution caused by phthalates is escalating, with dimethyl phthalate (DMP) emerging as one of the most prevalent contaminants within the phthalates (PAEs) category. Although the biodegradation of DMP is considered both safe and efficient, its underlying degradation mechanism is not yet fully elucidated, and the degradation performance can be somewhat inconsistent. To address this issue, our study isolated a DMP-degrading bacterium (DNM-S1) from a vegetable greenhouse. The resulting data revealed that DNM-S1 exhibited a remarkable degradation performance, successfully degrading 84.98% of a 2000 mg L −1 DMP solution within 72 h. Remarkably, it achieved complete degradation of a 50 mg L −1 DMP solution within just 3 h. DMP degradation by DNM-S1 was also found to be efficient even under low-temperature conditions (10 °C). Our research further indicates that DNM-S1 is capable of capturing DMP through the ester bond in the bacterium's cell wall fatty acids, forming hydrogen bonds through hydrophobic interactions. The DMP was then transported into the DNM-S1 protoplasm using an active transport mechanism. Interestingly, the secondary metabolites of DNM-S1 contained natural carotenoids, which could potentially counteract the damaging effects of PAEs on cell membrane permeability. In summary, these findings highlight the potential of DNM-S1 in addressing PAEs pollution and provide new insights into the metabolic mechanism of PAEs degradation.
研究领域:
环境微生物学
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生物修复
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酞酸酯降解
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微生物代谢
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2018
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IF 5.091
Shuai Ma,Lifeng Dong,Hongzhou Dong,Jie Wang,Yingjie Chen,Beili Pang,Jianguang Feng,Liyan Yu,Mei Zhao
We concretely report feasible synthesis procedures of colloidal Cu 2 ZnSn(S 1- x ,Se x ) 4 -Au (CZTSSe-Au) nano-heterostructured composites, and creatively employ them as the counter electrodes (CEs) of all-solid-state solar cells with inorganic CsSnI 2.95 F 0.05 perovskite hole conductor. Acquired optical characterization indicates that integration of noble metal nanoparticles with cuprum-chalcogenide could heighten light absorption within visible-band due to localized surface plasmon resonance (LSPR) generated by Au, and the forbidden gap of nanocomposites gets adjustment accordingly. It is demonstrated that this novel photocathode alternative with favorable conductivity can not only match the energy level within the device band structure construction, but also restrain recombination so that accelerate charge transfer and extraction occurring on the photocathode. The photocurrent and photoelectric conversion efficiency (PCE) of cells conjugating CZTS-Au photocathodes turn to be respectively 43% and 25% higher than those using pure CZTS. Moreover, it has been demonstrated that CZTS-Au, coupling very well with inorganic perovskite, owns comparable electrocatalytic performance and even higher output photocurrent with respect to platinum CEs, which portends a potential substitution for conventional costly photocathodes. A comprehensive analysis on impedance spectroscopy data is subsequently carried out for the sake of deep understanding charge accumulation and transfer response at CsSnI 2.95 F 0.05 /CE interface, attempting to orient further optimization of device performance.
研究领域:
材料科学
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能源科学
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Kun Wang,Meng-meng Niu,Yue Wu,Rui-fang Zhu,Jing Zhao,Bao-xin Lu,Guang-cai Niu
This study investigated the physicochemical characteristics and antioxidant activities of cell-bound exopolysaccharides (c-EPS) from Lactobacillus fermentum S1 extracted using either phenol (CFP) or ethylenediaminetetraacetic acid (CFE). The extraction products, CFP and CFE, were homogeneous polysaccharides with molecular weights of 2.89 × 10 5 and 2.44 × 10 5 Da, respectively. Both CFP and CFE were composed of five kinds of monosaccharides. They had a main chain of β-D-Glc p -(1→2), α-L-Rha p -(1→2), β-D-Gal p -(1→3), α-D-Glc p- (1→3), α-D-Gal p -(1→3) and β-L-Rha p- (1→3,4) residues, and a tail of α-D-Man p -(1→) residue, but with different molar ratios. Trace amounts of D-Gal p- (1→) and L-Ara f -(1→2,5) residues were randomly distributed in the sugar chains. Although both CFP and CFE were in triple-helical conformation, they differed from each other in relation to surface morphology and thermal properties. The two c-EPS had different performances in radical scavenging and ferric reducing antioxidant power assays in vitro . The antioxidant capacity of both c-EPS extracts was confirmed in vivo using Caenorhabditis elegans , whose total antioxidant capacity and total superoxide dismutase activity increased after c-EPS treatment. Overall, the extraction method significantly affected the structure and biological activity of c-EPS. Moreover, the potent antioxidant activities of CFP and CFE support their use as natural antioxidants in dietary supplements or food additives.
研究领域:
微生物学
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食品科学
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生物化学
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DOI
高影响因子
Journal of Future Foods
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2025
·
IF 7.2
Jian Wang,Xiangwen Chen,Faming Yang,Chenxue Zhang,Hao Wu,Junxiang Zhu
Enhancing the gel properties of surimi is critical for advancing aquatic protein product quality. This study demonstrates that hydroxycinnamic acids (HCAs), such as caffeic acid (CA), p -coumaric acid (PCA), chlorogenic acid (CGA), and rosmarinic acid (RA), significantly improve the texture, water-holding capacity, and flavor of silver carp ( Hypophthalmichthys molitrix ) surimi and myosin gels. RA incorporation resulted in the most significant increase in gel strength and WHC, without compromising whiteness. To elucidate the mechanistic basis of these improvements, the interactions between HCAs and myosin S1 were investigated. Microscale thermophoresis revealed RA as the strongest binder to myosin S1 ( k d = 2.33 mM), followed by CA (9.25 mM), CGA (37.29 mM), and PCA (109.50 mM). Thermodynamic analyses and molecular simulations confirmed spontaneous binding mainly driven by hydrogen bonding or hydrophobic forces. HCA binding induced divergent structural effects: RA and CGA stabilized α-helical structures, enhancing intermolecular alignment of stabilized helices, while CA and PCA promoted partial unfolding, exposing hydrophobic residues. Both pathways elevated aggregation propensity, as evidenced by increased particle size and radius of gyration. Microrheology further demonstrated RA’s superior capacity to enhance myosin S1 elasticity and viscosity, correlating with its robust gel network formation. These findings elucidate how HCA-myosin S1 interactions underpin the observed improvements in gel properties, establishing RA as a potent natural modifier for surimi. The work provides a molecular blueprint for leveraging polyphenol-protein interactions to optimize texture, stability, and sensory attributes in aquatic products.
研究领域:
食品科学
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生物化学
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分子生物学
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水产品加工
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DOI
高影响因子
Fermentation-Basel
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2021
·Vol. 7
·期 4
·309
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IF 3.975
Yiming Sun,Xiaowei Lin,Shaodong Zhu,Jianmeng Chen,Yi He,Yao Shi,Hua Liu,Lei Qin
The biotrickling filter (BTF) treatment is an effective way of dealing with air pollution caused by volatile organic compounds (VOCs). However, this approach is typically used for single VOCs treatment but not for the mixtures of VOC and volatile organic sulfur compounds (VOSCs), even if they are often encountered in industrial applications. Therefore, we investigated the performance of BTF for single and ternary mixture gas of dimethyl sulfide (DMS), propanethiol, and toluene, respectively. Results showed that the co-treatment enhanced the removal efficiency of toluene, but not of dimethyl sulfide or propanethiol. Maximum removal rates (rmax) of DMS, propanethiol and toluene were calculated to be 256.41 g·m−3·h−1, 204.08 g·m−3·h−1and 90.91 g·m−3·h−1, respectively. For a gas mixture of these three constituents,rmaxwas measured to be 114.94 g·m−3·h−1, 104.17 g·m−3·h−1and 99.01 g·m−3·h−1, separately. Illumina MiSeq sequencing analysis further indicated that Proteobacteria and Bacteroidetes were the major bacterial groups in BTF packing materials. A shift of bacterial community structure was observed during the biodegradation process.Keywords:biotrickling filter;decomposition;dimethyl sulfide;pollution;propanethiol;toluene
研究领域:
环境工程
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微生物学
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空气污染控制
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DOI
高影响因子
危险品化学品经营许可证（带存储）