Food & Function
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2025
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IF 5.4
Qi Gu,Litao Wang,Mingyue Xu,Wan-Mei Zhou,Guosheng Liu,Haiting Tian,Thomas Efferth,Chenlu Wang,Yujie Fu
Phloretin is a natural dihydrochalcone with a variety of biological activities. Its potential in modulating lipid metabolism in vitro and in vivo with HepG2 and C. elegans was investigated in this study. Phloretin significantly diminished lipid accumulation and ROS levels, mitigated the degree of lipid peroxidation, and ameliorated mitochondrial dysfunction in HepG2 cells. The expression levels of lipid synthesis genes (SREBP and FASN) were downregulated, while PI3K-AKT related genes (AKT, FOXO, MTOR) were upregulated. Furthermore, phloretin significantly alleviated the detrimental effects of lipid accumulation on growth indices and locomotor ability in C. elegans. It also reduced lipofuscin, ROS level as well as glucose and triglyceride contents, significantly affected amino acid and lipid metabolism pathways based on metabolomics profiles and analysis. Gene expression levels of sbp-1, mdt-15, fat-5, fat-6, and fat-7 were markedly down-regulated following treatment with phloretin, while daf-16, age-1 and skn-1were significantly up-regulated. Tests with mutant C. elegans strains of daf-16, age-1, sbp-1 and nhr-49 further confirmed that the phloretin-induced decrease in lipid accumulation was attributed to the inhibition of lipid biosynthesis pathway by IIS and sbp-1/SREBP. Our study indicated that phloretin exerted hypolipidemic effects in vitro and in vivo, providing a basic foundation for considering phloretin as a phytochemical candidate for regulating lipid metabolism and preventing hyperlipidemia.
研究领域:
代谢生物学
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分子生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Batteries-Basel
·
2024
·Vol. 10
·期 3
·102
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IF 4.6
Xingyun Zhang,Kailimai Su,Yue Hu,Kaiyuan Xue,Yan Wang,Minmin Han,Junwei Lang
The zinc anode mainly faces technical problems such as short circuits caused by the growth of dendrite, low coulomb efficiency, and a short cycle life caused by side reactions, which impedes the rapid development of aqueous zinc-ion batteries (AZIBs). Herein, a common ionic liquid, 1,1-Spirobipyrrolidinium tetrafluoroborate ([SBP]BF4), is selected as a new additive for pure ZnSO4electrolyte. It is found that this additive could regulate the solvation sheath of hydrated Zn2+ions, promote the ionic mobility of Zn2+, homogenize the flux of Zn2+, avoid side reactions between the electrolyte and electrode, and inhibit the production of zinc dendrites by facilitating the establishment of an inorganic solid electrolyte interphase layer. With the 1% [SBP]BF4-modified electrolyte, the Zn||Zn symmetric cell delivers an extended plating/stripping cycling life of 2000 h at 1 mA cm−2, which is much higher than that of the cell without additives (330 h). As a proof of concept, the Zn‖V2O5battery using the [SBP]BF4additive shows excellent cycling stability, maintaining its specific capacity at 97 mAh g−1after 2000 cycles at 5 A g−1, which is much greater than the 46 mAh g−1capacity of the non-additive battery. This study offers zinc anode stabilization through high-efficiency electrolyte engineering.
研究领域:
材料科学
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电化学
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能量存储
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2018
·
IF 2.831
Xin Liu,Dan Wang,Jinshen Chu,Yang Xu,Wenjun Wang
Prostate-Specific Antigen (PSA) is a crucial biomarker for screening prostate cancer, but a sensitive and selective immunosensor for rapid quantification of serum PSA remains to be developed. In this study, a sandwich pair of nanobodies (Nbs) (i.e., Nb2 and Nb40) against PSA surface antigen was obtained from an alpaca-derived immune phage display library. A sandwich-type immunosensor for the sensitive and selective detection of PSA in serum samples was ingeniously designed based on the pair of Nbs. The small size of Nb40 allowed high capture densities on the surface of reduced graphene oxide (rGO) nanocomposed with massive Au nanoparticles ( [email protected] ), which significantly improved the conductivity and provided a large area to anchor many primary antibodies. The secondary antibody Nb2 fused with streptavidin -binding peptide (SBP) cooperated with Nb40 for PSA sandwiching. Accompanying introduction of horseradish peroxidase-streptavidin (HRP-SA) coupled with Nb2-SBP, the faradaic current was linearly correlated with the logarithm of PSA concentration in a range of 0.1–100 ng mL −1 . More importantly, this immunosensor exhibited excellent selectivity, stability, and reproducibility due to the sandwich pair Nbs. The proposed immunosensor was successfully applied in determining PSA in serum samples and could be used for the sensitive and specific detection of PSA.
研究领域:
生物技术
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纳米技术
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免疫学
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癌症研究
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2018
·
IF 8.173
Kaige Zhao,Min Tang,Huaisheng Wang,Zhenxian Zhou,Yafeng Wu,Songqin Liu
An immunosensing biochip for simultaneous detection of three biomarkers related to acute myocardial infarction (AMI) was developed based on anionic soybean peroxidase (SBP) functionalized nanoprobe and chemiluminescent imaging. The nanoprobes (Ab2-SiO 2 -SBP) were fabricated by co-immobilization of SBP and one of the detection polyclonal antibodies , anti-cardiac troponin I antigen (anti-cTnI), anti-creatine kinase-MB (anti-CK-MB) and anti-myoglobin (anti-Myo), on the silica nanoparticle surface. The detection sensitivity was enhanced since the large surface area of silica carriers increased the loading of SBP for per sandwiched immunoreaction. The immunosensing biochip designed as 3 × 8 wells array was constructed by simultaneously immobilizing three capture monoclonal antibodies on the same one microtiter well with 2 × 3 active spots. In the presence of target protein, the nanoprobes will be attached onto the spots with high specificity through the sandwiched immunoreactions, which triggered the chemiluminescence (CL) signals on each sensing site of the microtiter plates and allowed to CL imaging of three biomarkers in one well at the same time. Therefore, the proposed biochip was a promising convenient strategy for simultaneous detection of cTnI, CK-MB and Myo, which showed potential application for multianalyte determination in clinical diagnostics.
研究领域:
生物医学工程
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临床诊断
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纳米技术
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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