TALANTA
·
2022
·
IF 6.556
Yaqin Zhang,Xiangyu Quan,Yingchun Li,Hangyu Guo,Fange Kong,Jiahui Lu,Lirong Teng,Jiasi Wang,Di Wang
The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) proteins-based platforms have been used for the detection of pathogens. However, in further applications and research, due to multiple steps needed, many methods showed an increased risk of cross-reactivity. The thermostable Cas12b enables the combination of isothermal amplification and CRISPR-mediated detection, which could decrease the risk of cross-contamination. In this study, we developed a portable and specific diagnostic method that combined the gold nanoparticle (AuNP) with thermal stable CRISPR/Cas12b-enhanced reverse transcription loop-mediated isothermal amplification (RT-LAMP), which is called SCAN, to distinguish the N gene of SARS-CoV-2 from flu gene. We validated our method using RNA from cells transfected by plasmids. We could easily distinguish the positive results by the naked eye based on the strong molar absorption coefficient of AuNP. Moreover, SCAN has the potential for high-throughput tests owing to its convenient operation. In sum, SCAN has broken the site and equipment restrictions of traditional detection methods and could be applied outside of hospitals and clinical laboratories, greatly expanding the test of COVID-19.
研究领域:
分子生物学
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生物技术
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病毒学
·
诊断医学
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DOI
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Xu Tang,Zhijun Xie,Jiancheng Yu,Kewei Chen,Huanming Wu,Shifu Hu,Roozbeh Zarei,Keqi Tang
While portability is a key aspect of small mass spectrometers, they also need to be able to effectively screen the analytes from complex matrices. The accuracy and performance of mass spectrometers depend not only on the identification of parent ion signals in targets but also fragment ion signals. This paper reports on the construction of a device that uses a new time-domain excitation waveform called qualitative pre-scan waveform (QPSW) for its quadrupole ion trap mass spectrometry analyzer. This approach uses scanning to eliminate interfering ions, thus improving the sensitivity for the analyte and removing the cumbersome steps required to convert conventional stored waveform inverse Fourier transform (SWIFT) waveforms from the frequency domain to the time domain. Tests show that the target ions can be fragmented just by introducing air at the moment when the pinch valve is opened without any need for a carrier gas. This simplifies the equipment required, making the mass spectrometer more portable. Tests were undertaken to compare the performance with the use of conventional SWIFT waveforms and to optimize its experimental parameters so that the target ions were effectively accumulated in the ion trap. An approximate 5-fold improvement in sensitivity was achieved by QPSW compared to the SWIFT for 2 μg/mL ketamine. A 5-fold reduction in the limit of detection (LOD) to 20 ng/mL was also obtained for enrofloxacin. In addition, the stability was improved by QPSW with a relative standard deviation less than 13% compared to 16% for SWIFT.
研究领域:
分析化学
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仪器科学与技术
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
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IF 1.5
Weiqiang Meng,Peng Liu,Pingru Cai,Tingting Hao,Shaohua Ma,Yufang Hu,Sui Wang,Yangbo Wu,Zhiyong Guo
This work reported an approach for the determination of the heavy metal ion cadmium Cd(II) using a bismuth-film electrode (Bi-FE) with fast-scan anodic stripping voltammetry (FSASV). The experiment was divided into two steps. First, the Bi-FE was prepared by electrodepositing a metallic bismuth-film onto the electrode, and then the target metal ion Cd(II) was electrochemically preconcentrated on the Bi-FE. Second, the target metal was measured with FSASV. At a scan rate of 400 V/s, the anodic stripping peak current increased gradually with increasing Cd(II) concentration, and a linear relationship between the peak current and the logarithm of Cd(II) concentration was obtained from 0.1 μmol/L to 1.0 pmol/L, with a detection limit of 0.3 pmol/L (S/N=3). Furthermore, the method was successfully applied to detect Cd(II) in spiked tap water samples, with sufficient recoveries in the range of 94.0% to 105.2% and corresponding relative standard deviations ranging from 2.9% to 6.4%. Therefore, FSASV is a simple, rapid and ultrasensitive method for the detection of picomolar levels of Cd(II).
研究领域:
分析化学
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环境科学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2016
·Vol. 39
·期 15
·3054-3061
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IF 2.741
Min Li Xu,Bao Qiong Li,Xue Wang,Jing Chen,Hong Lin Zhai
Although liquid chromatography with mass spectrometry in full scan mode can obtain all the signals simultaneously in a large range and low cost, it is rarely used in quantitative analysis due to several problems such as chromatographic drifts and peak overlap. In this paper, we propose a Tchebichef moment method for the simultaneous quantitative analysis of three active compounds in Qingrejiedu oral liquid based on three-dimensional spectra in full scan mode of liquid chromatography with mass spectrometry. After the Tchebichef moments were calculated directly from the spectra, the quantitative linear models for three active compounds were established by stepwise regression. All the correlation coefficients were more than 0.9978. The limits of detection and limits of quantitation were less than 0.11 and 0.49 μg/mL, respectively. The intra- and interday precisions were less than 6.54 and 9.47%, while the recovery ranged from 102.56 to 112.15%. Owing to the advantages of multi-resolution and inherent invariance properties, Tchebichef moments could provide favorable results even in the situation of peaks shifting and overlapping, unknown interferences and noise signals, so it could be applied to the analysis of three-dimensional spectra in full scan mode of liquid chromatography with mass spectrometry.
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Xiaohao Sun,Chao Peng,Qi Wang,Wendong Cheng,Zhiqiang Lai,Baoan Fan,Yang Gui,Lan Yuan,Fen Guo
The electro-oxidation of monoethanolamine (MEA) is relevant from fundamental and applied, viz. sewage treatment, fuel cell and electrochemical water splitting, perspectives. Understanding mechanistic details of thorough electrocatalytic oxidation of MEA is of paramount importance for the rational design of high-performing electrocatalysts. Herein, in order to easily and quickly quantify the electro-oxidation degree of MEA and therefore assist the mechanism study, the present paper proposes an innovative strategy for determining the number of electrons transferred in the electro-oxidation of intermediates, with the aid of ultramicroelectrode (UME) and fast-scan cyclic voltammetry (CV). Deconvolutions on two fast-scan CV oxidation peaks, one of which is originated from the electro-oxidation of MEA to glycine intermediate and the other is the further electro-oxidation of intermediate, reveal that the number of electrons transferred in the electro-oxidation of glycine intermediates is merely 3.04±0.75 on pure Au catalyst, indicating the incomplete conversion of glycine to ammonia and glyoxylate ion. The introduction of trace Ni into Au electrocatalyst can remarkably boost the number of electrons transferred, an indication of more thorough electro-oxidation of glycine intermediates and thereby more bio-friendly degradation products. Theoretical calculations based on the density functional theory elucidate that such increase in electro-oxidation degree is due to the stronger adsorption of glycine intermediates on AuNi binary sites than on sole Au sites.
研究领域:
电化学
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催化科学
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环境科学
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理论计算
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DOI
高影响因子
OPTICS AND LASERS IN ENGINEERING
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2024
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IF 3.5
Shang Li,Zhihua Zeng,Xuan Su,Yingke Wang,Junyi Gu,Jie Xu,Bin Guo
Slippery surface, as the most cutting-edge function surface, has aroused the extensive attention ascribed to excellent hydrophobicity and self-cleaning property. Micro/nanostructure (functional carrier) and lubricant fluid (functional layer) collectively constitute slippery bi-phase surface. To our best knowledge, we firstly validate the preparation feasibility of lubricant-infused silicone rubber surface (LISRS) based on nanosecond laser splicing scan. Comprehensive characterizations demonstrate that laser splicing traces will break the uniformity of microstructures resulting in droplet pinning. However, the lubricant infusion can significantly endow the whole microstructure surfaces with droplet depinning effect, which directly improves the process compatibility of the laser splicing machining. The capillary infiltration and unstable loss of the lubricant on the microstructure surfaces not only confirm the stable lubricant layer of LISRS conformal with microstructures, but also reveal capillary stabilization mechanism of microgroove structures under gravity environment. Then, after being subjected to comprehensive stability tests (including mechanical abrasion, water droplet and jet impacts), the water droplet sliding behaviors of impacted surfaces show that the lubricant-infused microstructure surface has a certain degree of resistance to external impacts. These results show that it is extremely feasible to create large-scale LISRS using a laser splicing scan. With the rapid development of laser micro/nanofabrication, it is promising to break through the tough issue of large-scale and high-efficiency preparation of slippery silicone rubber surfaces, and bring slippery surfaces to the practical applications of anti-corrosion, anti-fouling and anti-icing materials or products.
研究领域:
材料科学
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表面工程
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激光加工
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DOI
高影响因子
ACS Omega
·
2022
·
IF 4.132
Qiaoling Yang,Bing Niu,Shuqing Gu,Jinge Ma,Chaomin Zhao,Qin Chen,Dehua Guo,Xiaojun Deng,Yongai Yu,Feng Zhang
To develop a rapid detection method for nonprotein nitrogen adulterants, this experiment sets up a set of point-scan Raman hyperspectral imaging systems to qualitatively distinguish and quantitatively and positionally analyze samples spiked with a single nonprotein nitrogen adulterant and samples spiked with a mixture of nine nonprotein nitrogen adulterants at different concentrations (5 × 10–3 to 2.000%, w/w). The results showed that for samples spiked with single nonprotein nitrogen adulterants, the number of pixels corresponding to the adulterant in the region of interest increased linearly with an increase in the analyte concentration, the average coefficient of determination (R2) was above 0.99, the minimum detection concentration of nonprotein nitrogen adulterants reached 0.010%, and the relative standard deviation (RSD) of the predicted concentration was less than 6%. For the sample spiked with a mixture of nine nonprotein nitrogen adulterants, the standard curve could be used to accurately predict the additive concentration when the additive concentration was greater than 1.200%. The detection method established in this study has good accuracy, high sensitivity, and strong stability. It provides a method for technical implementation of real-time and rapid detection of adulterants in milk powder at the port site and has good application and promotion prospects.
研究领域:
食品安全检测
·
光谱学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Qi Wang,Tao Wang,Han Lin,Weiqiang Meng,Chunfeng Zhang,Pingru Cai,Tingting Hao,Yangbo Wu,Zhiyong Guo
ABSTRACT A disposable Faraday cage-type aptasensor for ultrasensitive determination of sub-picomolar Hg(II) was developed based on fast scan voltammetry (FSV). Signal unit [email protected] was designed, in which aminoferrocene (AFc) is the signal label, gold nanoparticles (AuNPs) improve the conductivity of rGO and combine DNA through Au-S bond, DNA could selectively capture Hg(II) with DNA3 through the formation of thymine-Hg(II)-thymine (T-Hg(II)-T) complex, and rGO provides the basis for loading above functional components. In presence of Hg(II), the signal unit could be immobilized on the disposable electrode through T-Hg(II)-T mismatch to build a Faraday cage-type aptasensor, and the scan rate of 400 V/s was used by FSV, resulting in ultrasensitive determination of Hg(II) in concentration range from 0.1 μmol/L to 0.1 pmol/L with a limit of detection (LOD) 0.03 pmol/L. The sensitivity is greatly improved due to the following points: a large number of signal labels AFc are immobilized, conductive rGO allows all AFc immobilized to participate in the electrode reaction, and a high scan rate is used. Satisfied stability, selectivity and accuracy were obtained. Thus, this proposed disposable Faraday cage-type aptasensor provides a new path to ultrasensitive on-site rapid determination of Hg(II) or analog heavy metal ions.
研究领域:
分析化学
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环境科学
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纳米技术
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电化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Fuxing Xu,Weimin Wang,Li Ding,Xiang Fang,Chuan-Fan Ding
Development of fragment ion detection methods is of great importance for mass spectrometer advancement or substance identification. To date, collision induced dissociation (CID) remains the most commonly used ion activation method in MS/MS experiments, and the effectiveness of CID in an ion trap mass spectrometer is limited by low mass cutoff and weak fragmentation yields. Theoretically, controlling the q value is the key to maintain the fragment efficiency and trapping efficiency of MS/MS, thus improving the detection of fragment ion, while currently reported techniques usually require complex circuitry and often produce different CID patterns. In this paper, with the developed synchronized reversed scanning–collision induced dissociation (SRS-CID) technique, we demonstrate its effective improvement in fragment ion detection. The SRS-CID is implemented on a digital ion trap mass spectrometer (DITMS) by reverse scanning the q values during CID process, or specifically, the frequency is increased during the CID process. With the SRS-CID technique, the fragmentation efficiency of precursor ions can be slightly improved. For reserpine analyte, the trapping efficiency for low-mass fragment ions is improved at least 3 times, and for YGGFL, the trapping efficiency for low-mass fragment ions is improved at least 9 times. These experimental results can also be validated by simulations, and the kinetic energy variation plot suggests consecutive fragmentation occurs. In any case, the SRS-CID provides a solution to the low efficiency of fragment ion detection during tandem MS analysis, which will certainly be useful in the future.
研究领域:
分析化学
·
质谱学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Ning Guo,Chun-Yan Peng,Quan-Fei Zhu,Bi-Feng Yuan,Yu-Qi Feng
Carbonyl compounds are considered as the potential biomarkers for oxidative stress and many types of diseases; therefore their determination may serve as indicator for early clinical diagnosis. Here we developed a strategy based on isotope labeling combined with liquid chromatography-double precursor ion scan-mass spectrometry (IL-LC-DPIS-MS) analysis for comprehensive profiling and relative quantitation of carbonyl compounds in human serum. First, we chose labeling reagents (2-(2-hydrazinyl-2-oxoethyl)isoquinolin-2-ium bromide, HIQB; N , N , N -triethyl-2-hydrazinyl-2-oxoethanaminium bromide, THB; Girard reagent T, GT; Girard reagent P, GP), all of which contain reactive group, isotopically labeled moiety and ionizable group to selectively label carbonyl compounds. Since HIQB labeling offered the best detection sensitivities for carbonyl compounds among these labeling reagents, we used HIQB and the corresponding isotope-labeled reagent of d 7 -HIQB as the optimal isotope-labeled reagent. The HIQB and d 7 -HIQB labeled carbonyl compounds can generate two characteristic product ions of m/z 130.1/137.1 under collision-induced dissociation (CID), which contain an isotope tag and therefore were used for double precursor ion scans in mass spectrometry analysis. Using this strategy, 156 carbonyl compounds candidates were detected in human serum, 12 of which were further identified by commercial standards. Subsequently, a targeted method using multiple reaction monitoring (MRM) detection mode was developed for relative quantification of carbonyl compounds in human serum from myelogenous leukemia (ML) patients and healthy controls. As a result, 44 carbonyl compounds were found to have significant difference between ML patients and healthy controls, suggesting that these carbonyl compounds may play certain roles in ML and also can serve as indicators for ML. Taken together, the isotope labeling combined with tandem mass spectrometry analysis demonstrated to be a powerful strategy for identification and quantification of carbonyl compounds in serum samples.
研究领域:
分析化学
·
医学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Advanced Optical Materials
·
2016
·Vol. 4
·期 11
·1732-1737
·
IF 5.359
Wen-Gao Lu,Cheng Chen,Dengbao Han,Linhua Yao,Junbo Han,Haizheng Zhong,Yongtian Wang
Graphical The comparison between organic–inorganic CH 3 NH 3 PbBr 3 quantum dots and all inorganic halide perovskite CsPbBr 3 quantum dots allows to understand the structural difference and corresponding optical properties. Using Z-scan measurements, it is revealed that colloidal CH 3 NH 3 PbBr 3 exhibits enhanced two-photon absorption properties compared to those of CsPbBr 3 quantum dots due to their structural difference.
研究领域:
材料科学
·
光学
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产品关联
DOI
高影响因子
ANALYST
·
2014
·Vol. 139
·期 13
·3446-3454
·
IF 3.906
Yun-Qing Huang,Qiu-Yi Wang,Jia-Qi Liu,Yan-Hong Hao,Bi-Feng Yuan,Yu-Qi Feng
We developed a novel method for non-targeted screening of metabolites by high performance liquid chromatography-mass spectrometry with paired homologous double neutral loss scan mode after in vitro isotope labelling (IL-HPLC-PHDNL-MS). As a proof of concept, we investigated the carboxylic acid metabolite profiling in plant samples by the IL-HPLC-PHDNL-MS method. To this end, N,N-dimethylaminobutylamine (DMBA) and d4-N,N-dimethylaminobutylamine (d4-DMBA) were synthesized and utilized to label carboxylic acids. Our results show the MS response of carboxylic acids was enhanced by 20- to 40-fold after labelling. As for the IL-HPLC-PHDNL-MS analysis, DMBA and d4-DMBA labelled samples were mixed equally before MS analysis. Because the isotope labelled moieties (dimethylamino moiety, Me2N) of DMBA and d4-DMBA are easily ruptured and lost as neutral fragments (NL 45 and NL 49) under collision induced dissociation (CID), two neutral loss scans can be carried out simultaneously to record the signals of DMBA and d4-DMBA labelled samples, respectively. In this respect, the metabolites from two samples labelled with different isotope reagents are ionized at the same time but recorded separately by mass spectrometry, which can eliminate the MS response fluctuation and mutual interference. Using this method, six potential biomarkers involved in wounded tomato leaves were identified, and their structures were further elucidated by product ion scan and high resolution mass spectrometry analysis. Taken together, the IL-HPLC-PHDNL-MS method demonstrated good performance on the identification as well as relative quantification of metabolites with a carboxyl group in biological samples.
研究领域:
分析化学
·
代谢组学
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DOI
高影响因子
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