MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Zhirou Xu,Zhengtong Wu,Xuyang Sun
An ultrasensitive fluorescence immunosensor based on silver nanoparticles@ nitrogen-doped carbon quantum dots (Ag NPs@N/CQDs) and exploiting the quenching effect of gold nanoparticles (Au NPs) was fabricated for squamous cell carcinoma antigen (SCCA) detection. Ag NPs@N/CQDs were immobilized on the surface of an indium tin oxide (ITO) electrode and then coupled with the primary antibody of SCCA. The primary antibody captured SCCA on the ITO electrode, forming an immune complex with the secondary antibody labeled with gold nanoparticles, thereby constructing a sandwich-structured fluorescent sensor. Using the prepared sensor, a new method for detecting trace amounts of SCCA in human serum was established. Owing to the effective quenching of fluorescence intensity of Ag NPs@N/CQDs by Au NPs, combined with the presence of a sandwich immune structure, the sensor exhibits high sensitivity and selectivity. The method demonstrates a linear range of 2 − 4000 fg mL −1 and a detection limit of 0.87 fg mL −1 . The the recovery rate for real serum samples was between 94.45 % to 103.43 %.
研究领域:
生物医学工程
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肿瘤学
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纳米技术
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DOI
高影响因子
IEEE SENSORS JOURNAL
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2023
·Vol. 23
·期 7
·6728-6733
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IF 4.3
Wentao Liu,Fangrong Hu,Shangjun Lin,Lizhen Zeng,Mingzhu Jiang,Longhui Zhang,Yingchang Zou
Squamous cell carcinoma antigen (SCCA) level is an auxiliary indicator for lung cancer diagnosis and predictive monitoring, and its standard reference value is less than 1.5 ng/mL. Therefore, achieving high sensitivity and specific detection of the SCCA is a challenge. In this work, we designed a double split-ring-resonator (SRR) terahertz (THz) metasurface sensor. Simulation results show that the sensitivity of the sensor can reach 103 GHz/RIU (refractive index unit). After the sensor is modified with different concentrations of halloysite nanotubes (HNTs), the resonant frequency of the sensor exhibits different linear responses to different concentrations of SCCA. The optimum concentration of HNTs to enhance the detection performance of the sensor was obtained as 10 mg/mL. The linear range of SCCA concentration of the HNT modified sensor was 1 pg/mL to 100 ng/mL. On this basis, we modified anti-SCCA on the sensor, and other biomarkers of carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), CA125, and CA199 were used as comparisons. The results indicated that the sensor modified with HNTs showed a more remarkable frequency shift for the SCCA, which verified that the sensor had good specificity for the SCCA. Therefore, this sensor can be used for highly sensitive and specific detection of the SCCA.
研究领域:
生物医学工程
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癌症诊断
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纳米技术
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传感器
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Lixia Luo,Jinglin Shui,Yingying Gu,Dandan Wang,Jiangyang Qin,Yanan Wang,Yong Mei
In order to detect the cervical cancer biomarker squamous cell carcinoma antigen (SCCA) in human serum, an electrochemical immunosensor was constructed. Zirconium 1,4-dicarboxybenzene MOF (UiO-66), with a high specific surface area, was coupled with gold nanoparticles (AuNPs), creating the UiO-66@AuNPs nanomaterial. This coupling increased the sensitivity of the electrochemical immunosensor by providing many biological binding sites for antibodies to bind to Au-S bonds. Then, mesoporous carbon (CMK-3) was added to the UiO-66@AuNPs nanomaterial to obtain a CMK-3@UiO-66@AuNPs nanocomposite. This further increased the electron transfer proficiency of the material and enhanced the electrochemical signal. Finally, the sensitive detection of squamous cell carcinoma antigen (SCCA) was made possible by unique immunological responses between antigens and antibodies. Transmission electron microscopy (TEM) analysis of CMK-3@UiO-66@AuNPs revealed embedded surfaces and voids; cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful assembly of the electrochemical immunosensor. The developed electrochemical immunosensor demonstrated good linearity (R 1 2 = 0.999) from 0.01 pg/mL to 10,000 pg/mL, which is the concentration range of SCCA in human serum. The limit of detection was 0.0034 pg/mL (at an S/N ratio of 3). The immunosensor exhibited excellent precision, stability and selectivity, with a high rate of recovery (98.26–101.8 %). The experimental validation studies demonstrated that the electrochemical immunosensor was effective for the detection of SCCA in human serum, offering a novel method for the early diagnosis and dynamic surveillance of cervical cancer.
研究领域:
生物医学工程
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癌症研究
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电化学
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纳米技术
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分析化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Song Ge,Wang Jiaqing,Jiang Xianrui,Han Hongliang,Ma Zhanfang
Nonspecific adsorption compromises the accuracy of electrochemical immunosensors in complex biological samples. Conventional antifouling materials often suffer from poor conductivity, which reduces sensitivity. To address this, a cascade-driven dual-signal attenuation strategy was designed. A hydrophilic conductive composite (GO/AuNPs/PPy/PA/MB, GAPPM) was synthesized via a one‑pot method to fabricate the sensing interface, and capture antibodies were anchored by electrodeposited AuNPs. The immunoprobe was constructed by combining GOx‑encapsulated ZIF‑8 with labeling antibodies linked via in‑situ grown AuNPs. Upon target antigen binding, a sandwich immunocomplex was formed on the electrode surface, and two cascade pathways were activated by glucose: (i) degradation of methylene blue signal molecules was induced, leading to attenuation of the current, and (ii) generation of an insoluble coordination precipitate was promoted, increasing interfacial resistance. Dual‑path cascade signal amplification was thus achieved synergistically. Using squamous cell carcinoma antigen as a model, the immunosensor achieved a detection limit of 29.5 fg mL⁻ 1 and a wide linear range (1 pg mL⁻ 1 to 1 µg mL⁻ 1 ). Notably, after 30-min incubation in undiluted human serum, the modified electrode exhibited an impedance change 56% lower than that of its BSA‑blocked counterpart, confirming excellent antifouling properties. This strategy holds great promise for high‑performance electrochemical biosensing in complex biological samples. Graphical
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhi Luo,Jing Li,Shenglan Hu,Lingling Zheng,Lixin Xu,Biyang Deng
In this study, Ru(bpy) 3 2+ and oxalate (C 2 O 4 2− ) in Ru@Zn-MOF generated self-enhanced electrochemiluminescence (ECL). The self-enhanced ECL composite exhibited a short electron transfer distance and minimal energy loss, which ensured the high and stable luminescence efficiency of Ru@Zn-MOF. A MnO 2 @AuNPs nanocomposite was introduced into Ru@Zn-MOF via antigen–antibody interactions in the presence of squamous cell carcinoma antigen (SCCA). This significantly quenched the ECL signal due to the combined effect of the dual quenchers, MnO 2 NFs and AuNPs. A highly efficient ECL-RET immunosensor was constructed for the sensitive detection of SCCA in the linear range of 1 fg/mL to 100 ng/mL, with a limit of detection of 0.26 fg/mL (S/N = 3). This immunosensor has broad clinical applications for the quantitative detection of biomarkers.
研究领域:
生物医学工程
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分析化学
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肿瘤学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Yuanyuan Liu,Hongmin Ma,Jian Gao,Dan Wu,Xiang Ren,Tao Yan,Xuehui Pang,Qin Wei
In this study, a novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of squamous cell carcinoma antigen (SCCA) based on the β-cyclodextrin functionalized graphene nanosheet (CD-GN) and the ternary hollow Pt/PdCu nanocube anchored on three-dimensional graphene framework (Pt/PdCu-3DGF). CD-GN exhibited high specific surface area and good dispersibility and stability in water, which were beneficial to fix captured antibodies (Ab 1 ) through the supramolecular host-guest interaction between CD and Ab 1 . The abundant oxygen-containing functional groups on 3DGF provided binding sites for anchoring noble metal nanoparticles . Pt/PdCu-3DGF could capture detected antibodies via the interaction of Pd-NH 2 and Pt-NH 2 . Furthermore, the ternary metal nanoparticles exhibited high electrocatalytic activity toward the reduction of hydrogen peroxide. Under optimal conditions, the fabricated immunosensor showed a sensitive response to SCCA with two linear ranges. The linear ranges are 0.0001–1 ng/mL and 1–30 ng/mL with a detection limit of 25 fg/mL. Additionally, the proposed immunosensor showed good reproducibility and stability.
研究领域:
生物化学
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医学检测
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纳米技术
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2014
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IF 4.195
Qianying Zhao,Ruiqi Duan,Jialing Yuan,Yi Quan,Huan Yang,Mingrong Xi
Squamous cell carcinoma antigen (SCCa), as a tumor biomarker, plays an important role in adjuvant diagnosis, treatment evaluation, and prognosis prediction for cervical cancer patients. Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles bypasses the disadvantages of traditional testing strategies, in terms of free-labeling, short assay time, good sensitivity, and selectivity. Herein, we develop a novel and reusable LSPR biosensor for the detection of SCCa. First, a triangle-shaped silver nanoparticle array was fabricated using the nanosphere lithography method. Next, we investigated and verified the feasibility of amino coupling method using 11-mercaptoundecanoic acid (MUA) to form a functionalized chip surface with monoclonal anti-SCCa antibodies on the silver nanoparticles for distinct detection of SCCa. Different concentrations of SCCa were successfully tested in both buffer and human serum by the ultrasensitive and specific LSPR system, with a linear quantitative detection range of 0.1–1,000 pM under optimal conditions. With appropriate regeneration solution, for example 50 mM glycine-HCl (pH 2.0), the LSPR biosensor featured effective fabrication reproducibility, which reduced both production cost and testing time. Our study represents the first application of the LSPR biosensor in cervical cancer, and demonstrates that the rapid, simple, and reusable nanochip can serve as a potential alternative for clinical serological diagnosis of SCCa in cervical cancer patients.
研究领域:
生物医学工程
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癌症诊断
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纳米技术
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaoxue Ye,Mingshi Li,Ye Hu,Zhengguo Wang,Yanying Wang,Hongping Deng,Xiaoxing Xiong,Chunya Li,Dong Sun
A photoelectrochemical immunosensor was fabricated for squamous cell carcinoma antigen (SCCA) assay with high performances by using MoSe 2 nanosheets and hollow gold nanospheres as photosensitive materials. MoSe 2 nanosheets were simply synthesized with a hydrothermal method using 1,3-di-(3-thioacetylpropyl)-imidazolium bromide (DITAPIBr) ionic liquid as a morphological controlling reagent, selenium powder, and sodium molybdate as raw materials. Hollow gold nanospheres (HGNs) were prepared by using 1,3-di-(3-bromopropyl)-imidazolium bromide ionic liquid (DIBPIBr) as functional monomer, and NaBH 4 as a reductant to interact with the HAuCl 4 solution. Subsequently, HGNs were integrated with MoSe 2 nanosheets to provide a HGNs-MoSe 2 nanocomposite. The photocurrent intensity of MoSe 2 nanosheets was significantly enhanced by the hybridization of HGNs. Furthermore, HGNs-MoSe 2 nanocomposites were coated onto a glassy carbon electrode surface to construct a platform for the immobilization of SCCA antibody (anti-SCCA), resulted in a photoelectrochemical immunosensing system for determining SCCA. Under optimized conditions, the fabricated immunosensor shows high selectivity and sensitivity towards SCCA, and exhibits a detection limit of 0.21 pg mL −1 and a linear range from 1.0 pg mL −1 to 50 ng mL −1 . Meanwhile, the practicability of the immunosensor was demonstrated by determining SCCA in clinical serum samples with high accuracy.
研究领域:
生物传感器
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纳米技术
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癌症诊断
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光电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）