SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Xueyu Chang,Yaqian Cheng,Xinke Wang,Yonghui Wang,Xueli Liu,Tie Han,Zhixian Gao,Huanying Zhou
A fluorescent biosensor strategy was developed in combination with immunomagnetic separation for rapid and sensitive detection of staphylococcal enterotoxin B (SEB). Magnetic nanoparticles (MNPs) modified with aptamer of SEB could capture the SEB. Then the gold nanoparticles (AuNPs) fluorescent probe was added and a “sandwich structure” was formed between AuNPs, SEB and MNPs. The MNPs-SEB-AuNPs structure could be separated with an additional magnetic field, which resulted the lower signals of AuNPs fluorescent probe. In optimal conditions, the current method displayed a broad quantitative range from 100 to 10 7 fg/mL and the limit of detection was 3.43 fg/mL. The recovery of SEB-spiked milk samples ranged from 92.00 to 119.00 %, which revealed that the developed method had great accuracy. Furthermore, the method was fast and economical for ultrasensitive detection. Therefore, the fluorescent biosensor based on MNPs-AuNPs is promising for the detection of other environmental and food pollutants.
研究领域:
生物技术
·
食品科学
·
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Huiping Feng,Yue Sun,Jiang Wang,Jiwei Xu,Xihui Mu,Bin Du,Zhaoyang Tong,Bing Liu
Staphylococcal enterotoxin B (SEB) is a major pathogenic factor in foodborne diseases, and its rapid and sensitive detection is crucial for early warning of food safety risks and public health protection. In this study, a colorimetric immunosensing system for SEB detection was constructed based on a magnetic covalent organic framework (COF) composite loaded with platinum nanoparticles (Pt NPs) that exhibit dual enzymatic activities. The effective loading of Pt NPs endows the composite with excellent peroxidase (POD) and oxidase (OXD)-like activities. The COF matrix not only provides abundant binding sites for Pt NP dispersion but also enhances substrate adsorption, thereby significantly improving catalytic efficiency. By introducing an Fe3O4 core, the nanocomposite enables facile preparation of antibody–nanoparticle conjugates through separation and enrichment functions. The Fe3O4@COF@Pt nanocomposite demonstrates excellent peroxidase- and oxidase-like activities, capable of decomposing H2O2 and O2 to generate hydroxyl radicals (˙OH) and superoxide anions (O2˙−), which catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (OXTMB), resulting in a visible color change for signal output. The sensor achieves specific detection by utilizing its peroxidase-mimicking activity, exhibiting a linear detection range of 1–200 ng mL−1 with a detection limit as low as 0.395 ng mL−1. This nanozyme-linked immunosorbent assay (NLISA) based on Fe3O4@COF@Pt offers a highly sensitive and convenient platform for SEB detection.
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Beibei Lu,Jialei Bai,Jingyang Zhang,Huijing Shen,Minglin Wang,Yujing Lian,Zhixian Gao,Yuan Peng
Colorimetric sensing materials have a rising demand in detection applications, especially in the field of naked-eye detection. Herein, a novel iridescent polymeric film that can respond to different Staphylococcus aureus enterotoxin B (SEB) concentrations in the visible range is reported. This film is fabricated by polymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EGDMA) owing an inverse opal array (IOA) structure, and gold nanoparticles (AuNPs) are enchased on the polymer skeleton. This composite structure facilitates the film to respond to SEB at a LOD of 4.29 fg mL −1 , making it the best performing colorimetric material to date. Cryo-Scanning Electron Microscopy and 3D AFM were used to observe the morphology change of the iridescent polymeric film after SEB binding. This film possesses great advantages for ultrasensitive visual detection of real samples. In addition, the iridescent polymeric film has the potential to become a universal sensing material, realizing the ultrasensitive visual detection of hazardous substances without equipment in the field by replacing the recognition element.
研究领域:
材料科学
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分析化学
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生物传感
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纳米技术
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产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Lei Liu,Zhifang Ma,Qiaoyi Han,Wei Meng,Hongbo Ye,Tianci Zhang,Yu Xia,Zehong Xiang,Yue Ke,Xinghua Guan,Qiang Shi,Fazly I. Ataullakhanov,Mikhail Panteleev
The brain and liver are more susceptible to ischemia and reperfusion (IR) injury (IRI), which triggers the reactive oxygen species (ROS) burst and inflammatory cascade and results in severe neuronal damage or hepatic injury. Moreover, the damaged endothelial barrier contributes to proinflammatory activity and limits the delivery of therapeutic agents such as some macromolecules and nanomedicine despite the integrity being disrupted after IRI. Herein, we constructed a phenylboronic-decorated chitosan-based nanoplatform to deliver myricetin, a multifunctional polyphenol molecule for the treatment of cerebral and hepatic ischemia. The chitosan-based nanostructures are widely studied cationic carriers for endothelium penetration such as the blood–brain barrier (BBB) and sinusoidal endothelial barrier (SEB). The phenylboronic ester was chosen as the ROS-responsive bridging segment for conjugation and selective release of myricetin molecules, which meanwhile scavenged the overexpressed ROS in the inflammatory environment. The released myricetin molecules fulfill a variety of roles including antioxidation through multiple phenolic hydroxyl groups, inhibition of the inflammatory cascade by regulation of the macrophage polarization from M1 to M2, and endothelial injury repairment. Taken together, our present study provides valuable insight into the development of efficient antioxidant and anti-inflammatory platforms for potential application against ischemic disease.
研究领域:
纳米技术
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药学
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神经科学
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肝病学
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炎症研究
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 6
·1255-1261
·
IF 2.667
Wu Zhengzong,He Deyun,Cui Bo,Jin Zhengyu,Xu Enbo
Reliable and sensitive detection of Staphylococcal enterotoxin B (SEB) is important to prevent potential human health risk from foods contaminated by SEB. Here, a triple-channel assay was developed for ultrasensitive detection of SEB. Based on the optical characteristics of tetramers composed of gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs), the SEB amounts in food products can be simultaneously detected by using surface-enhanced Raman scattering (SERS), fluorometry, and circular dichroism (CD). Under SERS mode, fluorescence mode, and CD mode, wide linear detection ranges (1–750 pg/mL, 1–750 pg/mL, and 2–500 pg·mL −1 ) were achieved. Furthermore, the practicality of the novel assay was evaluated by determining SEB contents in spiked milk samples. These results indicate that the triple-mode aptasensor is a potential useful tool in quantitative detection of SEB in foods.
研究领域:
食品科学
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分析化学
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纳米技术
·
毒理学
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DOI
高影响因子
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