RSC Advances
·
2023
·Vol. 13
·期 51
·36098-36106
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IF 3.9
Meng Cao,Feng Xu,Li Li,Honglian Zhang,Xin Liang,Tao Xu,Qian Li
The objective of this study was to establish a novel method for the detection of lean meat powder (salbutamol sulfate and terbutaline sulfate) residues in commercially available pig liver based on diazo coupling reaction by combining thin layer chromatography (TLC) with surface-enhanced resonance Raman scattering (SERRS). TLC was used to separate samples; after determining the spots of each component, diazo coupling reaction was carried out on the spots in situ to generate azo compounds, and then the spots of azo compounds mixed with silver sol on the TLC plate were qualitatively detected by SERRS. The limit of detection (LOD) of salbutamol sulfate was as low as 0.14 μg kg−1, and that of terbutaline sulfate was as low as 0.04 μg kg−1. The influence of the sample matrix in TLC-SERRS detection of salbutamol sulfate and terbutaline sulfate was investigated by experiment on a simulated positive sample, and salbutamol sulfate and terbutaline sulfate in commercially available pig liver were detected. Compared with the reported method (HPLC-MS/MS), this method has the advantages of strong specificity, high sensitivity, rapidity and low cost. It provides a new reference method for establishing and perfecting the safety system of veterinary drug residues.
研究领域:
食品安全
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分析化学
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兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Innovative Optical Health Sciences
·
2016
·
IF 1.033
Yunfei Zhang,Danqi Li,Xingyu Zhou,Xihui Gao,Shengyuan Zhao,Cong Li
Surface enhanced resonance Raman scattering (SERRS) is a physical phenomenon that occurs when the energy of incident light is close to that of electronic excitation of reporter molecules (RMs) attached on substrates. SERRS has showed great promise in healthcare applications such as tumor diagnosis, image-guided tumor surgery and real-time evaluation of therapeutic response due to its ultra-sensitivity, manipulating convenience and easy accessibility. As the most widely used organic near-infrared (NIR) fluorophore, heptamethine cyanines possess the electronic excitation energy that is close to the plasmon absorption energy of the gold nano-scaffolds, which results in the extraordinary enhancement of the SERRS signal. However, the effect of heptamethine cyanine structure and the gold nanoparticle morphology to the SERRS intensity are barely investigated. This work developed a series of SERRS nanoprobes in which two heptamethine cyanine derivatives (IR783 and IR780) were used as the RM and three gold nanoparticles (nanorod, nanosphere and nanostar) were used as the substrates. Interestingly, even though IR780 and IR783 possess very similar chemical structure, SERRS signal produced by IR780 was determined as 14 times higher than that of IR783 when the RM concentration was 6.5 × 10 −6 M. In contrast, less than 4.0 fold SERRS signal intensity increase was measured by changing the substrate morphologies. Above experimental results indicate that finely tuning the chemical structure of the heptamethine cyanine could be a feasible way to develop robust SERRS probes to visualize tumor or guide tumor resection with high sensitivity and target to background ratio.
研究领域:
生物医学工程
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分子影像学
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Congzheng Zhu,Jin Li,Xinru Chen,Longwen Fu,Zhiyang Zhang,Yunqing Wang,Xiaoyan Wang,Lingxin Chen
CXC chemokine receptor 4 (CXCR4) is recently regarded as a valuable biomarker for triple-negative breast cancer (TNBC) metastasis but lacks available imaging reagents. Surface-enhanced resonance Raman scattering (SERRS) with resonant dyes has emerged as a powerful tool for single-cell imaging because of the electronically enhanced vibrational fingerprint signals. However, resonant Raman signals are often overwhelmed by accompanying fluorescence backgrounds. To address this, two black hole quenchers (BHQs) are designed as visible resonance Raman reporters with absolutely nonfluorescent readouts. Ultrafast spectroscopy elucidates that the nonfluorescent mechanism of the reporters originates from the ultrafast internal conversion at the subpicosecond scale that quenches the excited states of fluorescence. SERRS nanoprobes (NPs) decorated with such reporters exhibit strong Raman enhancement (5.82 × 10 6 ), the femtomolar-level limit of detection as well as unrivaled photostability (τ s = 26516 s), outperforming that of crystal violet-decorated counterparts. When conjugation of a CXCR4 antagonist, these fluorescence-free SERRS NPs allow for photostable imaging of CXCR4 on TNBC cells at the single-cell level, and for monitoring the expression variation during combined drug treatment. To the best of the available knowledge, this is the first example of absolutely nonfluorescent Raman reporters for single-cell SERRS imaging.
研究领域:
生物医学工程
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癌症研究
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分子影像学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 12
·2906
·
IF 4.2
Hailin Wen,Miao Li,Chao-Yang Zhao,Tao Xu,Shuang Fu,Huimin Sui,Cuiyan Han
A highly sensitive, selective and recyclable histidine detection method based on magnetic Fe3O4@mTiO2(M-TiO2) nanocomposites with SERRS was developed. Mesoporous M-TiO2nanoparticles were functionalized with 4-aminothiophenol and then coupled with histidine through an azo coupling reaction in 5 min, producing the corresponding azo compound. The strong and specific SERRS response of the azo product allowed for ultrasensitive and selective detection for histidine with an M-TiO2device loaded with Ag NPs due to the molecular resonance effect and plasmonic effect of Ag NPs under a 532 nm excitation laser. The sensitivity was further enhanced with the magnetic enrichment of M-TiO2. The limit of detection (LOD) was as low as 8.00 × 10−12mol/L. The M-TiO2demonstrated applicability towards histidine determination in human urine without any sample pretreatment. Additionally, the M-TiO2device can be recycled for 3 cycles with the photodegradation of the azo product under UV irradiation due to TiO2-assisted and plasmon-enhanced photocatalysis. In summary, a multifunctional and recyclable M-TiO2device was synthesized based on azo coupling and SERRS spectroscopy for ultra-sensitive and specific histidine sensing. In addition, the proposed system demonstrated the potential for the multiplex determination of toxic compounds in the fields of food safety, industrial production and environmental protection, which benefit from the fingerprint property and universality of SERRS.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2017
·
IF 0
Zhigang Liu,Shihua Yu,Shuping Xu,Bing Zhao,Weiqing Xu
Discrimination of illegal cooking oil is a conundrum in the fields of analytical chemistry and food safety due to complicated sample systems, lack of common targets, and stringent demand of ultrahigh detection sensitivity for corresponding analytical methods. Capsaicin, one of the exogenous molecules that is subsistent in recycled kitchen waste oils, can be regarded as a target for illegal cooking oil identification. Nowadays, tracing capsaicin in oils is implemented mainly by high-performance liquid chromatography–mass spectrometry, which displays shortcomings in high costs and incapableness for field test. Here, we established a surface-enhanced resonance Raman scattering approach to detect capsaicin and identify illegal cooking oils by means of the molecular derivatization treatment of capsaicin. This method features high detection sensitivity with the detection limit of 1.0 × 10–8 M, rapid response (<7 min detection duration), and simplicity in sample pretreatment, which is available for fast field test of illegal cooking oils.
研究领域:
分析化学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Zhen Li,Mei Li,Cong Wang,Xinhui Zhou,Jianfeng Li,Daoliang Li
Based on modified diazo reaction, a novel surface-enhanced resonance Raman scattering (SERRS) method employed for ultrasensitive, highly selective, and ultra-trace determination of nitrite in aquaculture water was demonstrated. The azo dyes, which are formed by 4-aminothiophenol, nitrite, and N-(1-naphthyl) ethylenediamine dihydrochloride in acid conditions, can exhibit high SERS signal on Ag nanoparticles. Subsequently, the concentration of nitrite can be determined from the intensity of SERRS of derivative. Under optimal conditions, a linear relationship between Raman intensity and the nitrite concentration was found in the range of 0.1–15.0 μmol L −1 with a good correlation coefficient (R 2 = 0.9921) at 1380 cm −1 , and the limit of detection can reach as low as 16.4, 24.0, 9.0, and 24.6 nmol L −1 at 1138, 1332, 1380 and 1418 cm −1 , respectively. Additionally, the results indicate that the present method can be applied to detect nitrite in real aquaculture water samples without influence of interfering ions. This study provides a feasible method for accurate, trace, and fast field detection of nitrite in aquaculture water with strong potential to be used for on-line and on-site optical sensors of nitrites.
研究领域:
环境科学
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分析化学
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产品关联
DOI
高影响因子
ANALYST
·
2013
·Vol. 138
·期 9
·2624-2631
·
IF 3.969
Yuanyuan Chen,Hanwen Cheng,Kha Tram,Shengfeng Zhang,Yanhua Zhao,Liyang Han,Zengping Chen,Shuangyan Huan
In this study, a novel paper-based SERRS immunoassay based on magnetic separation and alkaline phosphatase (ALP) enzyme catalyzed hydrolysis reaction was developed. By using modified antibodies conjugated to magnetic beads, capture of mouse IgG followed by addition of ALP-labeled antibodies would form a sandwich-like immunoconjugate. After magnetic separation, 5-bromo-4-chloro-3-indolyl phosphate (BCIP), a low SERRS active compound, was added as the substrate for ALP to generate a high SERRS response. Detection was conducted on a silver colloid/PVP/filter paper SERS substrate by spotting a pre-aggregated silver colloid sol onto polyvinyl pyrrolidone (PVP) modified filter paper using a semi-automatic TLC sample applicator. The optimization of the highly SERS active paper-based substrate, dynamic hydrolysis process of BCIP, quantitative detection of IgG, and selectivity of the assay was studied in detail. By taking advantage of magnetic separation in order to decrease the background interference, the selective enzyme reaction involved in producing a highly SERRS active product could detect mouse IgG from 1 to 500 ng mL−1 with a LOD of 0.33 ng mL−1.
研究领域:
分析化学
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生物传感器
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免疫分析
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光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Li Li,Feng Xu,Ge Sun,Mingrui Sun,Shoushi Jia,Hongmei Li,Tao Xu,Honglian Zhang,Yan Wang,Yue Guo,Taohua Liu
The objective of this study was to establish a novel method for the determination of N-methylaniline (NMA) based on azo coupling reaction in infant pacifiers prepared with food contact silicone materials by combining thin layer chromatography (TLC) with surface-enhanced resonance Raman scattering (SERRS). TLC was used to separate the azo reaction products to confirm the component spot of azo compound, then the spot of azo compound mixed with silver sol on the TLC plate was qualitatively detected by SERRS. The limit of detection (LOD) of the method is as low as 0.50 ppm for NMA. The influence of sample matrix about the TLC-SERRS detection of NMA was investigated by experiment of simulated positive sample, and the NMA in infant pacifiers exposed to silica gel products was detected. The method of TLC-SERRS for the determination of NMA in infant pacifiers prepared with food contact silicone materials was established, and the real samples were detected. Compared with the methods ever reported, the method has the advantages of high sensitivity, specificity and low cost. It provides a new reference method for establishing a safety system for food contact silicone materials.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Bao-Ying Wen,Jing-Wen Zhou,Wen-Xiang Jiang,Yue-Jiao Zhang,Jian-Feng Li
Bilirubin, as a major component of human bile and a byproduct of red blood cell metabolism, plays an essential role in diagnosing jaundice and assessing liver function. Surface-enhanced resonance Raman spectroscopy (SERRS) is a highly sensitive detection technique that emerged from the synergy of resonance Raman spectroscopy and surface-enhanced Raman spectroscopy (SERS). This technology not only provides molecular fingerprint information but also offers rapid detection, interference resistance, and high selectivity, making it particularly suitable for specific biological system detection. Herein, we have developed an innovative SERRS technique for efficiently measuring bilirubin levels in urine. This method involves precisely adjusting the pH of sodium nitrite to 1, oxidizing bilirubin to biliverdin. Subsequently, a portable Raman spectrometer with an excitation wavelength of 785 nm is used to emit its resonance Raman signal, achieving high-sensitivity and selective rapid detection. Through this approach, we have achieved the quantification of a wide linear dynamic range spanning from 860 nmol/L to 34.2 μmol/L, characterized by an excellent correlation coefficient of 0.99 and a remarkably low detection limit of 860 pmol/L, which is significantly below that of traditional detection methods. Moreover, in the analysis of real samples, minimal pre-treatment is required to achieve high sensitivity and expedited detection. The entire procedure is completed in merely 1 min, with recovery rates falling between 80 % and 100 %. This approach paves the way for the specific and rapid detection of biomarkers using Raman spectroscopy.
研究领域:
医学诊断
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光谱学
·
生物分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）