TALANTA
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2023
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IF 6.1
Tingting Zhang,Gongying Wang,Juan Bao,Chang Liu,Weihua Li,Zhe Kong,Xiuyun Sun,Jiansheng Li,Rui Lu
A crucial issue in analytical science and physiology is the detection of histamine with high sensitivity, specificity and credibility, which served as an important neurotransmitter in biofluids. Despite the high sensitivity of surface-enhanced Raman spectroscopy (SERS) at the level of single molecule, there are still challenges in providing high sensitivity for histamine with a small cross section. For the selective detection of histamine using SERS, a highly sensitive sandwich structure substrate combining Fe 3 O 4 and an Ag-based SERS nanotag was developed. The Fe 3 O 4 @SiO 2 –COOH served as a capture component for enriching histamine. Upon functionalized Ag nanoparticles with glycine (Gly) and (3-Aminopheyonyl) boronic acid (APBA), they were then used to connect with histamine and serve as a SERS nanotag, respectively. A linear relationship between the Raman intensity and the histamine concentration was observed over the range 10 −4 -10 −8 M with a limit of detection of 7.24 × 10 −9 M. This methodology also exhibited good selectivity in the presence of other neurotransmitters. With our new approach, histamine can be detected sensitively and reliably in fish samples, which indicates the potential prospect of an effective method for analyzing histamine in complex specimens.
研究领域:
分析化学
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生物医学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Dechan Lu,Yanping Chen,Longfeng Ke,Zufang Huang,Yudong Lu,Jing Wang
DNA methylation is a promising biomarker for early cancer detection; however, traditional detection methods require bisulfite treatment and nucleic acid amplification, which are costly and impractical for point-of-care use. We propose a facile method that leverages immunoassays and surface-enhanced Raman scattering (SERS) readouts for the multiplex, sensitive, and portable detection of lung cancer-related DNA methylation biomarkers. This approach employs 5-methylcytosine antibody-modified magnetic probes to enrich methylated DNA sequences and uses core–shell nanoparticles with Probe DNA as SERS nanotags for multiplex detection, eliminating the need for bisulfite treatment and nucleic acid amplification. The multiplex SERS immunoassay detects synthetic methylated SHOX2 and RASSF1A with detection limits of 0.52 pM and 0.66 pM, respectively. It shows a strong correlation with quantitative PCR results in analyzing methylated SHOX2 and RASSF1A from cell lines and formalin-fixed paraffin-embedded tissue samples (n = 35). Additionally, the random forest analysis based on methylated SHOX2 and RASSF1A expression distinguishes lung cancer from benign lung diseases with a clinical sensitivity of 96 %, specificity of 90 %, and an AUC value of 0.972. This method offers a significant advancement for DNA methylation research and clinical applications.
研究领域:
癌症生物学
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分子诊断学
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纳米技术
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生物化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2021
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IF 6.558
Lunjie Huang,Da-Wen Sun,Zhihui Wu,Hongbin Pu,Qingyi Wei
Efficient identification of pathogenic bacteria is greatly concerned with microbial food safety and foodborne diseases diagnosis. Surface-enhanced Raman scattering (SERS) tags are among the cutting-edge tools for bioanalysis, but facing troubles in either SERS sensitivity, durability, interfering signals, or universal recognition agents for target bacteria. This work proposed a multivariate scheme enabled by polyphenolic chemistry for the green synthesis, facile stabilization (functionalization), protective encapsulation, and bio-affinitive design of metal-phenolic networks (MPNs)-encapsulated silver SERS nanotags ( [email protected] @MPNs). With remarkable SERS properties, shelf stability, and bacterial affinity, [email protected] @MPNs tags enabled rapid, reproducible, and interference-free SERS detection of two representative foodborne pathogens ( i.e., E. coli O157: H7 and S. aureus ) in the assistance of an aptamer-labelled magnetic probe, reaching good sensitivity and selectivity. Moreover, this SERS biosensor worked well in real food samples, manifesting the potential of polyphenolic chemistry in the customization of bio-affinitive SERS nanotags for food safety detection.
研究领域:
生物技术
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分析化学
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食品科学
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微生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Ziwei Hu,Xia Zhou,Jun Duan,Xueqiang Wu,Jiamin Wu,Pengcheng Zhang,Wanzhen Liang,Jialiang Guo,Huaihong Cai,Pinghua Sun,Haibo Zhou,Zhengjin Jiang
The design of a rapid, simple, and ultrasensitive detection platform of protein biomarkers has become an urgent need in early diagnosis and treatment. Surface-enhanced Raman scattering (SERS) technology, with the unique advantages of ultra-sensitivity and fingerprint recognition, has attracted much attention in biomarker detection. An innovative aptamer SERS biosensor for highly sensitive protein biomarker detection was presented. This biosensor contains a SERS tag (reporter-labeled Au nano-bridged nanogaps particles, Au NNPs) and novel magnetic capture substrate (Ag-coated Fe 3 O 4 -Au NPs, Ag MNPs). C-reactive protein (CRP), a representative biomarker for diagnosing, was used to assess the SERS performance of this biosensor. Aptamers against CRP were modified on both SERS tag and magnetic capture substrate for specific recognition by Au NNPs-CRP-Ag MNPs sandwich assay. This strategy achieved sensitive detection of CRP with a limit of detection (LOD) of 10 fM (1.14 pg/mL), and is much lower than reported analytical methods. Besides, this method exhibits excellent selectivity and specificity for CRP under the interference of other proteins and displays high accuracy in the detection of actual human serum samples. This proposed detection strategy can be extended to the sensitive detection of other biomarker proteins and hold great promise in the clinical diagnosis of diseases.
研究领域:
生物传感
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纳米技术
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分析化学
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生物医学工程
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诊断医学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Xiaowei Cao,Shengjie Ge,Miao Chen,Haiyan Mao,Ying Wang
Multiplexed detection of Parkinson’s disease (PD) biomarkers is of great significance for early diagnosis and personalized treatment. In this study, we fabricated a robust surface-enhanced Raman scattering-enabled lab-on-a-chip (LoC-SERS) platform for simultaneous quantification of α-synuclein, phosphorylated tau protein 181, osteopontin, and osteocalcin. Herein, the antibody–DNA conjugate was designed to introduce the catalytic hairpin self-assembly (CHA) amplification into the protein detection. Au nano-stars (AuNSs) modified with Raman reporter molecules and hairpin-structure DNA 1 were applied as the SERS nanotags. Au-coated silicon nanocone array (Au/SiNCA) fabricated based on the maskless plasma etching-prepared high-density Si nanocone array (SiNCA) and surface ion sputtering was used as the capture substrate after the modification of hairpin-structure DNA 2. Benefitting from the antibody–DNA conjugate-induced CHA amplification, numerous AuNSs can be connected to the Au/SiNCA surface, which significantly amplify the plasmonic coupling effect for ultrasensitive SERS detection, and the limit of detection was less than the pg/mL level. The application of highly uniform Au/SiNCA and antibody–DNA conjugate endows the LoC-SERS platform excellent analytical performance, including superior reproducibility, satisfactory universality, and high sensitivity. In addition, a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice model was established, and satisfactory results were obtained in real sample analysis with the LoC-SERS platform, which may be enlightening for exploiting protein biomarkers in PD monitoring.
研究领域:
生物医学工程
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分析化学
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神经科学
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疾病诊断
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产品关联
DOI
PubMed
高影响因子
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