BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Yingying Jiang,Yu Cao,Yaoqi Li,Liuliu Bi,Lv Wang,Qianqian Chen,Yue Lin,Huanzhi Jin,Xiaoming Xu,Renyi Peng,Zheyan Chen
The incidence of diabetes is increasing annually, and the disease is uncurable due to its complex pathogenesis. Therefore, understanding diabetes pathogenesis and developing new treatments are crucial. This study showed that the NO donor SNP (8 µM) significantly alleviated high glucose-induced developmental toxicity in zebrafish larvae. High glucose levels caused hyperglycemia, leading to oxidative stress and mitochondrial damage from excessive ROS accumulation. This promoted mitochondrial-dependent apoptosis and lipid peroxidation (LPO)-induced ferroptosis, along with immune inflammatory reactions that decreased mitochondrial function and altered intracellular grid morphology, causing imbalanced kinetics and autophagy. After SNP treatment, zebrafish larvae showed improved developmental toxicity and glucose utilization, reduced ROS accumulation, and increased antioxidant activity. The NO-sGC-cGMP signaling pathway, inhibited by high glucose, was significantly activated by SNP, improving mitochondrial homeostasis, increasing mitochondrial count, and enhancing mitochondrial function. It's worth noting that apoptosis, ferroptosis and immune inflammation were effectively alleviated. In summary, SNP improved high glucose-induced developmental toxicity by activating the NO-sGC-cGMP signaling pathway to reduce toxic effects such as apoptosis, ferroptosis and inflammation resulting from mitochondrial homeostasis imbalance.
研究领域:
糖尿病
·
发育生物学
·
毒理学
·
斑马鱼
✓ 已验证
DOI
PubMed
高影响因子
CARBON
·
2020
·
IF 8.821
Bing Li,Shunli Shang,Jiawei Zhao,Daniil M. Itkis,Xingxing Jiao,Chaofan Zhang,Zi-Kui Liu,Jiangxuan Song
Potassium-ion batteries (PIBs) have attracted great interest due to their high-energy-density and low-cost. The lack of stable anode material greatly limits the quick development of PIBs. Phosphorus-metal compounds are regarded as a class of materials with promising prospects as anode material for PIBs with a low operating voltage and high conductivity. Among them, due to the challenging synthesis method , the application of SnP is limited. Herein, a facile approach to synthesize trigonal SnP@C through alloying red phosphorus with tin on carbon material is reported. It is found that carbon substrate can largely reduce vibrational and configurational entropies, playing a critical role on the formation of metastable SnP. When applied as anode in PIBs, the SnP@C composite delivers a high reversible capacity of 478.1 mAh·g −1 at 50 mA g −1 and a stable cycling performance at 1000 mA g −1 . The good electrochemical performance is associated with the SnP@C as well as the carbon, which could suppress the phase separation during charge/discharge process to maintain structural stability. This work may open a new avenue for low-cost synthesis of metastable phases for advanced energy storage systems .
研究领域:
材料科学
·
能源存储
✓ 已验证
DOI
高影响因子
Engineering
·
2022
·
IF 12.834
Chen Hu,Yanjie Hu,Aiping Chen,Xuezhi Duan,Hao Jiang,Chunzhong Li
Tin phosphides are attractive anode materials for ultrafast lithium-ion batteries (LIBs) because of their ultrahigh Li-ion diffusion capability and large theoretical-specific capacity. However, difficulties in synthesis and large size enabling electrochemical irreversibility impede their applications. Herein, an in situ catalytic phosphorization strategy is developed to synthesize SnP/CoP hetero-nanocrystals within reduced graphene oxide (rGO)-coated carbon frameworks, in which the SnP relative formation energy is significantly decreased according to density functional theory (DFT) calculations. The optimized hybrids exhibit ultrafast charge/discharge capability (260 mA·h·g −1 at 50 A·g − 1 ) without capacity fading (645 mA·h·g − 1 at 2 A·g − 1 ) through 1500 cycles. The lithiation/delithiation mechanism is disclosed, showing that the 4.0 nm sized SnP/CoP nanocrystals possess a very high reversibility and that the previously formed metallic Co of CoP at a relatively high potential accelerates the subsequent reaction kinetics of SnP, hence endowing them with ultrafast charge/discharge capability, which is further verified by the relative dynamic current density distributions according to the finite element analysis.
研究领域:
材料科学
·
能源存储与转换
✓ 已验证
产品关联
DOI
高影响因子
Diversity-Basel
·
2023
·Vol. 15
·期 6
·701
·
IF 2.4
Meile Sun,Xiangrong Ren,Ya Liu,Jing Yang,Jingtao Hui,Yukun Zhang,Yanhua Cui,Jun Zhang,Guocang Lin,Yan Li
Roses are important plants, and they are cultivated worldwide for their beautiful flowers, remarkable scent, and nutritious hips. In this study, we re-sequenced the whole genomes of 57 rose cultivars and one wild species that originated from different regions around the world and had different scents, aiming to evaluate their genetic structure and to detect the potential signature of the selective sweep between different scent groups with single-nucleotide polymorphism (SNP) and indel markers. The roses were sequenced at an average depth of 6× to the reference genome ofRosa‘Old blush’. A total of 2,375,949 SNPs were obtained. The SNP numbers varied among the 58 samples, with an average of 1,271,906 per sample. The phylogeny and population structure revealed that the roses could be divided into three main clusters; however, the groups were not consistent in terms of geographic origin or scent classification. These indicated that rose cultivars have a complex genetic background due to the extensive hybridization between cultivated roses or wildrosaspecies worldwide. A selective sweep analysis was conducted to detect the selection signatures in rose scent traits. A total of 2430 candidate genes were identified in the strong scent groups, and were at the top 5% FSTcompared to the moderate group. These genes were significantly enriched in the KEGG pathways of tyrosine metabolism, cyanoamino acid metabolism, alpha-linolenic acid metabolism (13 genes), and phenylpropanoid biosynthesis (30 genes). When the low-scent group was used as the control, 2604 candidate genes were identified at the top 5% FSTthat were significantly enriched in the KEGG pathways of alpha-linolenic acid metabolism (15 genes), and glutathione metabolism (23 genes). We also observed genes enriched in pathways including the linoleic acid metabolism (five genes), diterpenoid biosynthesis (six genes), and monoterpenoid biosynthesis (seven genes), although they were not significant. These implied a positive selection of rose cultivars with a strong fragrance in terms of fatty acid derivatives, terpenoids and benzenoids/phenylpropanoids during rose breeding. Our study establishes a foundation for the further large-scale characterization of rose germplasm, improving the genetic knowledge of the background of roses.Keywords:single-nucleotide polymorphism (SNP);indel;genome variation;selection sweep;KEGG pathway
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2017
·
IF 3.359
Nan Hu,Wei Liu,Lixue Jin,Yajie Li,Zhiqiang Li,Guimin Liu,Di Huang,Zhaoliang Wu,Hao Yin
The effective recovery of trace heavy metals from their wastewaters is an important but challenging work. Here, a promising process of nano-adsorption coupled with flotation had been validated for the recovery of trace Cu 2+ from its aqueous solution. Firstly, silica nanoparticles (SNPs) acted as an adsorbing carrier to adsorb Cu 2+ (15 mg/L). Subsequently, cetyl trimethyl ammonium bromide (CTAB) was utilized as a collector to capture SNPs. This process made it possible to combine the advantages of both nano-adsorption and flotation. Finally, the recovery efficiencies of Cu 2+ and SNP ( R SNP and R Cu ) and the volume ratio ( φ ) reached 97.1 ± 4.9%, 94.5 ± 4.7% and 23.8 ± 1.2, respectively under the suitable conditions of pH 6.0, SNP concentration 90 mg/L, CTAB concentration 35 mg/L, the adding time of CTAB 5 min and flotation column height 750 mm. By using this process, a suitable foam stability could be achieved because SNPs, apart from being an adsorbing carrier, also served as a foam stabilizer to significantly enhance the foaming stability. Furthermore, Cu 2+ was easily isolated from the recovered SNPs. Overall, this present work was highly effective for the recovery of trace Cu 2+ from its aqueous solution.
研究领域:
环境工程
·
纳米技术
·
水处理
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2012
·Vol. 5
·期 1
·285-290
·
IF 1.547
Xia Liu,Tao Li,Dianjun Liu,Zhenxin Wang
Here, a three-dimensional (3D) oligonucleotide microarray-based fluorescent assay has been developed for single nucleotide polymorphisms (SNPs) and allele frequency determination. The 3D oligonucleotide microarray is fabricated on a home-made polyacrylamide hydrogel microarray which is generated by UV light photopolymerization of tiny acrylamide monomer droplets on a PEG terminated glass slide. After activation by glutaraldehyde (GA), the polyacrylamide hydrogel is able to react with NH2-modified oligonucleotides with high efficiency to generate a high signal-to-background ratio without the conventional blocking step. We demonstrate that the assay is able to detect complimentary 25 mer oligonucleotide target (fragment of a tumor suppressor gene P53) down to 100 pM and SNP with true-to-false ratios (the ratio of fluorescence signal from perfect matched DNA strands to that from the one-base mismatched DNA strands) above 20. Taking advantage of the high specificity of the assay, allele frequency as low as 2% can be accurately determined.
研究领域:
生物化学
·
分子生物学
·
基因组学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2019
·Vol. 145
·期 1
·172-176
·
IF 4.019
Qian-Yu Zhou,Xin-Ying Zhong,Ling-Li Zhao,Li-Juan Wang,Ying-Lin Zhou,Xin-Xiang Zhang
Single nucleotide polymorphisms (SNPs) have been proven to be important biomarkers for disease diagnosis, prognosis and disease pathogenesis. Here, taking the advantages of a self-assembled oligonucleotide sandwich structure and robust chemical reactions, we have developed a simple, high-throughput and effective colorimetric analytical technique termed CuAAC-based ligation-assisted assays (CuAAC-LA) for SNP detection using a DNA-BIND 96-well plate. With the 5′-azide and 3′-alkyne groups labelled on two oligonucleotide probes, the target DNA can direct a Cu(I)-catalyzed alkyne–azide cycloaddition (CuAAC) click reaction. Since the small difference in duplex stability caused by a single-nucleotide mismatch was amplified by the steric effects of these reactive groups for the ligation reaction of an unstable duplex, CuAAC-LA exhibited an ultra-sensitive discrimination ability for a mutant type target in the presence of large amounts of wild type targets. As low as 0.05% SNP could be clearly detected, which was better than most previously reported methods by various DNA ligases, indicating that a simple and rapid synthetic method i.e., the DNA template-directed click reaction held the potential to replace the ligase for SNP detection.
研究领域:
分子生物学
·
基因组学
·
生物技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 136
·期 21
·4480-4485
·
IF 3.913
Shujuan Xu,Yong Shao,Kun Ma,Qinghua Cui,Guiying Liu,Fei Wu,Minjie Li
DNA single-nucleotide polymorphism (SNP) detection has attracted much attention due to mutation-related diseases. Various fluorescence methods for SNP detection have been proposed and many are already in use. However, fluorescence enhancement for signal-on SNP identification without label modification still remains a challenge. Here, we find that the abasic site (AP site) in a DNA duplex can be developed as a binding pocket favorable for the occurrence of the excited-state intramolecular proton transfer (ESIPT) of a 3-hydroxyflavone, fisetin, which is used as a proof of concept for effective SNP identification. Fisetin binding at the AP site is highly selective for target thymine or cytosine facing the AP site by observation of a drastic increase in the ESIPT emission band. In addition, the target recognition selectivity based on this ESIPT process is not affected by flanking bases of the AP site. The binding selectivity of fisetin at the AP site is also confirmed by measurements of fluorescence resonance energy transfer, emission lifetime and DNA melting. The fluorescent signal-on sensing for SNP based on this fluorophore is substantially advantageous over the previously used fluorophores such as the AP site-specific signal-off organic ligands with a similar fluorescing mechanism before and after binding to DNA with hydrogen bonding interaction. We expect that this approach will be employed to develop a practical SNP detection method by locating an AP site toward a target and employing an ESIPT probe as readout.
研究领域:
化学
·
生物化学
·
分子生物学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Fan Yang,JinBo Gong,Ming Li,Xiangyang Jiang,Jiawen Zhang,Meiyan Liao,Hanfei Zhang,Pier-Luc Tremblay,Tian Zhang
PCR-based techniques routinely employed for the detection of mutated linear DNA molecules, including circulating tumor DNA (ctDNA), require large nucleotide sections on both sides of the mutation for primer annealing. This means that DNA fragments with a mutation positioned closer to the extremities are unlikely to be detected. Thus, sensors capable of recognizing linear DNA with characteristic mutations closer to the ends would be advantageous over the state-of-the-art approaches. Here, an electrochemiluminescence-resonance energy transfer (ECL-RET) biosensor comprising capped CdS quantum dots and hairpin DNA probes labeled with Au nanoparticles was developed for the detection of epidermal growth factor receptor (EGFR) ctDNA carrying the critical T790M lung cancer mutation. The ECL-RET system detected different DNA molecules including single-stranded 18-nucleotides (nt) and 40-nt as well as double-stranded 100-nt with the single nucleotide polymorphism (SNP) coding for T790M located either in the middle or only 7 nt from one end. For all target DNA, the sensor’s limits of detection (LODs) were in the aM range, with excellent selectivity. It was the case of 100-nt target linear ctDNA fragments with LODs of 8.1 and 3.4 aM when the EGFR T790M SNP was either in the middle or at the end, respectively. These results show that ECL-RET systems can sense mutations in DNA fragments that would remain undetected by standard techniques.
研究领域:
生物传感器
·
纳米技术
·
癌症研究
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Hai-Bo Wang,Li-Hong Ma,Ting Zhang,Kai-Chen Huang,Yuan-Di Zhao,Tian-Cai Liu
Single nucleotide polymorphism (SNP) was associated with many human diseases, therefore, SNP detection was important for early diagnosis and clinical prognosis. Herein, a simple and accurate method for visual detection SNP sites (A/A, G/G, A/G) in CYP1A1 gene related to cancers based on colloidal gold nucleic acid strip biosensor and primer-specific polymerase chain reaction (PCR) was established. This method could directly distinguish SNP sites on strip biosensor by introducing twice PCR amplifications. The second PCR (primer-specific PCR) was performed using specific product of the first PCR as template, thus this twice PCR could reduce non-specific amplification greatly and obtain target product. In addition, single-strand or double-strand DNA (ssDNA or dsDNA) was accurately produced by introducing mismatched base at the 3’ end of forward primers in primer-specific PCR. The designed strip biosensor could only combine with the ssDNA, thus visual detection of SNP could be achieved within 10 min by color difference of a pair of strips. 61 human blood samples by this method were identical with those of pyrosequencing. This method had the advantages of rapid, visual and low-cost and was expected to be applied in medical diagnosis.
研究领域:
分子生物学
·
医学诊断
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Fang Hu,Wancun Zhang,Wei Meng,Yuxiang Ma,Xianwei Zhang,Ying Xu,Peng Wang,Yueqing Gu
Single nucleotide polymorphisms (SNPs) are crucial during the early diagnosis of a given disease as well as in evaluating their response to certain drugs. Thus, this study sought the development of ferrocene (Fc)-labeled electrochemical biosensor for SNP detection. This proposed system involves the ligation of four short probes (e.g., A, B, A′, and B′, where B′ is labeled with an Fc-tag) in the presence of target DNA via ligase chain reaction (LCR), resulting in the formation of Fc-tagged duplex AB-A′B′ in 2 n . Subsequently, immobilization of the Fc-tagged duplex AB-A′B′ on a single-stranded DNA capture probe (SC-DNA)-carboxyl multi-wall carbon nanotube (MWCNT-COOH) modified glassy carbon electrode (GCE) was accomplished through hybridization. Owing to the specificity of hybridization, and the use of Fc as electrochemical probe for detection of duplex AB-A′B′, such strategy realized directly analysis of LCR products without the need for purification . By taking advantage of the thermal stability and high-discrimination ability of HiFi Taq DNA ligase for single-base differences, the specificity of hybridization, the EGFR T790 M mutant DNA (MT-DNA) biosensor was developed to offer a low limit of detection (0.75 aM), a high discrimination of single-base mismatches [as low as 0.01% (molar fraction)], a wide linear range of more than 7 orders of magnitude (1 aM–10 pM), and the recovery rates (95.3%–107.8%) from human serum samples. Thus, the biosensor under development was found to be economical, highly-sensitive, and exceptionally selective for detection of SNPs, and as well as extending the versatile applications of LCR to offer great potential for diagnosis and individual clinical regimens.
研究领域:
生物化学
·
生物技术
·
分析化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Ruijiang Liu,Shuai Pan,Min Liu,Wei Huang,Zhixiang Lv,Aolin He
A label-free electrochemical biosensor with magnetically induced self-assembly based on peptide nucleic acids (PNA) and Fe 3 O 4 /Fe 2 O 3 heteroplasmon nanorods was successfully fabricated to detect the single nucleotide polymorphism (SNP) of CYP2C9*3. The sensitivity of the electrochemical biosensor was improved by introducing PNA and Fe 3 O 4 /Fe 2 O 3 @Au nanocomposites. The Fe 3 O 4 /Fe 2 O 3 @Au nanocomposites were assembled on the surface of the magnetic glassy carbon electrode (MGCE) by magnetic field, and the thiolated PNA were connected to the nanocomposites through Au-S bonds. The biosensor exhibited a good linear correlation between 1 pM and 1 µM (R 2 = 0.9941), and the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.95 pM and 3.18 pM, respectively. Besides, the electrochemical biosensor could recognize single-base mismatch DNA, and it could be stored stably for 15 days at 4 °C.
研究领域:
生物传感器
·
电化学
·
分子诊断
·
纳米技术
·
遗传学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）