Ovotransferrin (OVT) is a natural protein with various nutritional and biological benefits. However, further research is required to explore its effects and underlying mechanisms. To bridge this gap, conventional physiological methods with modern multi-omics approaches were employed to investigate the functional effects, metabolic targets and potential mechanisms of OVT, using Caenorhabditis elegans ( C. elegans ) as the model. Results revealed that OVT could improve oxidative stress and prolong lifespan in C. elegans by enhancing the activities of antioxidant enzymes and lowering levels of malondialdehyde, lipofuscin, and reactive oxygen species. Integrative analysis indicated that the upregulation of genes encoding Cytochrome P450 and sod-2 , and the increase in polyunsaturated fatty acids and salidroside should be closely associated with its effects in C. elegans . Moreover, enhanced intestinal mitochondria activity was observed in SJ4143 mutants. These results suggested that the action mechanisms of OVT in health promotion involved regulating lipid metabolism and boosting energy metabolism.
Acute lung injury (ALI) is a life threatening disease in critically ill patients, and characterized by excessive reactive oxygen species (ROS) and inflammatory factors levels in the lung. Multiple evidences suggest that nanozyme with diversified catalytic capabilities plays a vital role in this fatal lung injury. At present, we developed a novel class of polydopamine (PDA) coated cerium dioxide (CeO 2 ) nanozyme (Ce@P) that acts as the potent ROS scavenger for scavenging intracellular ROS and suppressing inflammatory responses against ALI. Herein, we aimed to identify that Ce@P combining with NIR irradiation could further strengthen its ROS scavenging capacity. Specifically, NIR triggered Ce@P exhibited the most potent antioxidant and anti-inflammatory behaviors in lipopolysaccharide (LPS) induced macrophages through decreasing the intracellular ROS levels, down-regulating the levels of TNF-α, IL-1β and IL-6, up-regulating the level of antioxidant cytokine (SOD-2), inducing M2 directional polarization (CD206 up-regulation), and increasing the expression level of HSP70. Besides, we performed intravenous (IV) injection of Ce@P in LPS induced ALI rat model, and found that it significantly accumulated in the lung tissue for 6 h after injection. It was also observed that Ce@P + NIR presented the superior behaviors of decreasing lung inflammation, alleviating diffuse alveolar damage, as well as promoting lung tissue repair. All in all, it has developed the strategy of using Ce@P combining with NIR irradiation for the synergistic enhanced treatment of ALI, which can serve as a promising therapeutic strategy for the clinical treatment of ROS derived diseases as well.
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