ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Mengyuan Qi,Xiaodan Ma,Bin Liang,Liying Zhang,Deyong Kong,Zhiling Li,Aijie Wang
Sulfonamide antibiotics (SAs) have been produced and consumed on a large scale over the last few decades. SAs are a typical class of refractory contaminants that are omnipresent in various environments. Although several [phenyl]-SA-degrading bacteria and their corresponding genomes have been documented, limited genetic information is available for the degraders of heterocyclic products (e.g., 3-amino-5-methylisoxazole [3A5MI] produced via sulfamethoxazole [SMX] catabolism). In this study, the previously isolated SMX-mineralizing bacterial partners, Paenarthrobacter sp. P27 (responsible for the initial cleavage of the -C-S-N- bond of SMX and further degradation of [phenyl]-SMX) and Norcardiodes sp. N27 (responsible for 3A5MI catabolism), were further studied and their complete genomes were sequenced. Complete degradation and bacterial growth were verified by pure-culture experiments with SMX or 3A5MI as the sole carbon, nitrogen, and energy source. By cross-feeding strains P27 and N27, complete catabolism of SMX could be achieved over a wide range of initial SMX concentrations. Moreover, strain P27 was capable of transforming the additional nine SA representatives into their corresponding nitrogen-containing heterocyclic products, strongly indicating the broad substrate spectrum and marked bioremediation potential of strain P27. The genome of strain P27 contained the highly homologous monooxygenase gene cluster, sadABC , which initially attacked the sulfonamide molecules. The complete genome sequences of the two important degraders will benefit future research centering on the molecular mechanism underlying advanced SMX mineralization and will aid in further understanding the interspecific interactions and metabolite exchanges for the optimization of artificially constructed synthetic functional microbiomes.
研究领域:
环境微生物学
·
基因组学
·
污染治理
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Zhenyu Zhai,Junfeng Su,Amjad Ali,Liang Xu,Fazli Wahid
This study emphasizes on the cellulase production characteristics of strain ZY7 and its collaboration with nitrate-dependent ferrous oxidizing (NFO) strain XL4 to achieve efficient denitrification at low carbon-to-nitrogen (C/N) ratio. Results indicated that the denitrification efficiency increased from 65.47 to 97.99% at 24 h after co-culture at C/N of 1.0. Three-dimensional fluorescence excitation-emission matrix (3D-EEM) showed significant changes in the intensity of soluble microbial products (SMP), fulvic-like materials, and aromatic proteins after co-culture. Bio-precipitates were characterized by Scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), and X–ray diffraction (XRD), which showed that cellulose structure was disrupted and the metabolites were potential carbon source for denitrification. In addition, cellulase activity suggested that the hydrolysis of β-1,4-glycosidic bonds and oligosaccharides may be the rate-limiting steps in cellulose degradation. This work promoted the understanding of denitrification characteristics of co-culture and expanded the application of cellulose degrading bacteria in sewage treatment.
研究领域:
微生物学
·
环境科学
·
污水处理
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Chang-Mei Li,Hai-Zhen Wu,Yu-Xiao Wang,Shuang Zhu,Chao-Hai Wei
The aim of this work is to study the synergistic effect of Stenotrophomonas sp. N5 and Advenella sp. B9 co-culture (COC) on enhancement of phenol biodegradation. These two strains utilizing phenol as sole carbon and energy source were isolated from phenol-containing coking wastewater. The results of biodegradation experiment showed the COC of N5 and B9 has stronger capability to degrade phenol than either of mono-culture (MOC). Growth kinetics studies indicated inhibitory effect of phenol on COC was reduced by the interaction of N5 and B9 in COC. The RNA-Seq results demonstrated that phenol biodegradation was enhanced by metabolic division of labor (DOL) in COC based on the expression of key genes for phenol degradation. GO enrichment analysis of differentially expressed genes (DEGs) indicated DEGs between COC and MOC degradation systems are mainly concentrated in the synthesis of cell components, microbial growth and metabolism, and catalytic activity. The expression of 3 transcriptional factors (LysR, Two-component system response regulator, and TetR families) which can regulate degradation of aromatic compounds, was identified beneficial to phenol degradation.
研究领域:
环境微生物学
·
生物降解
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2016
·
IF 4.836
Hui Zhang,Mengya Li,Jie Li,Guangli Wang,Feng Li,Dayong Xu,Yuan Liu,Minghua Xiong
A natural consortium, named L1, of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9 was obtained from quizalofop- p -ethyl (QE) polluted soil. The consortium was able to use QE as a sole carbon source for growth and degraded 100 mg L −1 of QE in 60 h. Strain JT-3 initiated the catabolism of QE to quizalofop acid (QA), which was used by strain JT-9 as carbon source for growth and to simultaneously feed strain JT-3. A novel esterase EstS-JT, which was responsible for the transformation of QE to QA and essential for the mineralization of QE by the consortium, was cloned from strain JT-3. EstS-JT showed low amino acid identity to other reported esterases from esterase family VIII and represents a new member of this family. The deduced amino acid sequence contained the esterase family VIII conserved motifs S-X-X-K, YSV and WAG. The purified recombinant EstS-JT displayed maximal esterase activity at 35 °C and pH 7.5. An inhibitor assay, site-directed mutagenesis and 3D modeling analysis revealed that S 64 , K 67 and Y 175 were essential for catalysis and probably comprised the catalytic center of EstS-JT. Additionally, EstS-JT had broad substrate specificity and was capable of hydrolyzing p -nitrophenyl esters (C 2 -C 8 ) and various AOPP herbicides.
研究领域:
环境科学
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2025
·
IF 3.7
Zhili Shi,Ya Li,Weibo Shi,Zhixin Mu,Qingxi Han,Weiwei Zhang
Probiotics are prior agents for treating bacterial infection with advantages of inhibiting pathogenic bacteria and improving immune responses of hosts, thus increasing the survival rate of cultured animals. In this study, one Vibrio parahaemolyticus YDE17 pathogenic to shrimp and its antagonist Glutamicibacter sp. ZY1 were screened, and ZY1 showed stable inhibitory effects on diverse Vibrio spp., especially V. parahaemolyticus. ZY1 secreted inhibitory substances into supernatant, and the activity of inhibitory substances did not change after being treated under different temperatures, proteinase K, and pH (6-10), which indicated that the inhibitory substances might be small molecules, which led us to trace the siderophore production. The siderophore production of YDE17 co-incubated with the cell-free supernatant of ZY1 was greater than that of YDE17 alone, which indicated that the cell-free supernatant of ZY1 created iron-limiting conditions for YDE17. This finding was confirmed by iron supplementation assays, in which the inhibitory activity of the cell-free supernatant of ZY1 on YDE17 as well as the siderophore production of YDE17 decreased in the presence of FeCl3. The effect of iron on inhibition was further confirmed by in vivo infection. The relative percent survival of ZY1 to shrimp challenged by YDE17 was 83.3%, but the survival rates of shrimp challenged with YDE17/ZY1/FeCl3 were similar to that of YDE17, both of which were significantly lower than the 70% survival rate of shrimps simultaneously challenged by ZY1/YDE17. Our study offers a new probiotic resource to control vibriosis, which works through iron competition with the opportunistic pathogens of Vibrio spp.
研究领域:
微生物学
·
水产养殖
·
病原生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Xiaoning Li,Hailong Liu,Weiben Yang,Hongjie Sheng,Fang Wang,Jean Damascene Harindintwali,H.M.S.K. Herath,Yinping Zhang
The search for nature-based tools to enhance bioremediation is essential for the sustainable restoration of contaminated ecosystems. Humic acid (HA) is an important component of organic matter in soil and water, but its effect on the microbial degradation of organic pollutants remains unclear. In this study, the biodegradation of pyrene by Mycobacterium sp. NJS-1 with and without HA was investigated. Only around 10.5% of pyrene was biodegraded in the pyrene treatment alone, whereas the addition of HA significantly enhanced biodegradation to the point where over 90% of pyrene was biodegraded. The production of 4,5-dihydropyrene-4,5-diol and phenanthrene-3,4-diol indicated the metabolic pathway via attacking of 4,5-positions of pyrene. Interestingly, 1,2-dimethoxypyrene was detected with the addition of HA, suggesting that HA induced a new ring-opening pathway involving the attack on the 1,2-positions of pyrene. The addition of HA first induced protein self-cleavage behavior with a significant increase in phenylalanine , tyrosine, and tryptophan containing large numbers of COO − groups. Furthermore, it altered the intracellular and extracellular ultrastructure of bacterial cells, promoting their growth in size and number as well as reducing the space between them. Overall, HA increased the ring-opening positions of pyrene and facilitated its interaction with bacterial cells, thus improving its biodegradability . Building upon the findings of this study to further research is conducive to the sustainable solution of environmental pollution .
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Qiang Zhang,Jian Xiao,Qing-Qing Sun,Jian-Chun Qin,Gennaro Pescitelli,Jin-Ming Gao
Bioassay-guided fractionation of the fermentation extract of Xylaria sp. XC-16, an endophyte from Toona sinensis led to the isolation of two new cytochalasans cytochalasin Z27, 1, and cytochalasin Z28, 2, along with three known compounds seco-cytochalasin E, 3, and cytochalasin Z18, 4, and cytochalasin E, 5. The structures of 1 and 2 were elucidated by spectroscopic and electronic circular dichroism methods. Compound 5 was shown to be potently cytotoxic against brine shrimp (LC50 = 2.79 μM), comparable to that of the positive agent toosendanin (LC50 = 4.03 μM), and also exhibited potential phytotoxic effects on Lactuca sativa and Raphanus sativus L. seedlings, which are higher than that of the positive control glyphosate. Additionally, the fungicidal effect of 2 against the phytopathogen Gibberella saubinetti was better than that of hymexazol. This is the first report of the three types of cytochalasins present in genus Xylaria. A structure–phytotoxicity activity relationship is also discussed.
研究领域:
真菌学
·
天然产物化学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2018
·Vol. 186
·期 2
·496-506
·
IF 1.797
Zhou Pei,Fang Ya-kun,Yao Hao-ke,Li Hua,Wang Gang,Liu Yu-peng
In this study, a method for the efficient production of dehydroepiandrosterone (DHEA) from phytosterols in a vegetable oil/aqueous two-phase system by Mycobacterium sp . was developed. After the 3-hydroxyl group of phytosterols was protected, they could be converted into DHEA with high yield and productivity by Mycobacterium sp . NRRL B-3683. In a shake flask biotransformation, 15.05 g l −1 of DHEA and a DHEA yield of 85.39% (mol mol −1 ) were attained after 7 days with an initial substrate concentration of 25 g l −1 . When biotransformation was carried out in a 30-l stirred bioreactor with 25 g l −1 substrate, the DHEA concentration and yield was 16.33 g l −1 and 92.65% (mol mol −1 ) after 7 days, respectively. The results of this study suggest that inexpensive phytosterols could be utilized for the efficient production of DHEA.
研究领域:
微生物学
·
生物化学
·
制药工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Jingwei Wang,Qiao Ma,Zhaojing Zhang,Catherine Sekyerebea Diko,Yuanyuan Qu
Buckminster fullerene (C 60 ), the most representative type among fullerenes, has attracted widely attentions because of its many potential applications. The increasing application of fullerene and limited knowledge of its environmental fate are required concerns. Herein, the biotransformation of C 60 by Labrys sp. WJW was investigated. Cell numbers reached 25.76 ± 1.85 folds within 8 days using 100 mg/L C 60 as sole carbon source. The biotransformation of C 60 by Labrys sp. WJW was analyzed by various characterization methods. Raman spectra indicated that strain WJW broke the soccer ball like structure of C 60 . After 12 days, over 60% of C 60 was degraded evidenced by UV–vis spectrophotometry and liquid chromatography-mass spectrometry. The underlying biotransformation mechanism of C 60 through an extracellular Fenton-like reaction was illustrated. In this reaction, the • OH production was mediated by reduction of H 2 O 2 involving a continuous cycle of Fe(II)/Fe(III). Bacterial transformation of C 60 will provide new insights into the understanding of C 60 bioremediation process.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2025
·Vol. 41
·期 5
·1-10
·
IF 4.2
Bi Yingjing,Han Yun,Bi Yanlan,Zhang Linshang,Sun Shangde
Sterol esters are naturally present in the by-product of edible oil processing, which is generally converted to sterols through saponification. Steroid intermediates play a crucial role in the production of pharmaceuticals, and these intermediates are predominantly synthesized via fermentation of sterol. This research explored the direct conversion of sterol esters into steroid intermediates using Mycobacterium sp. as a fermentation agent. The results demonstrated the successful identification of four steroid intermediates: androstenedione, 1,4-androstadienedione, 22-hydroxy-23,24-bisnorchol-4-ene-3-one, and 22-hydroxy-23,24-bisnorchol-1,4-dien-3-one, their individual intermediate concentrations were as follows: 144.2, 176.2, 30.8, 53.6 mg/L, with a total yield of 404.8 mg/L and a conversion rate of 29.5%. The optimized fermentation conditions included soybean oil at 3%, an initial pH of 7.0, a nitrogen source of 4.5 g/L, and hydroxypropyl-β-cyclodextrin of 15.0 g/L. Proteomic analysis revealed that sterol esters conversion pathway mirrors that of sterol, with an additional hydrolysis process. This work significantly expands our understanding of steroid intermediates production and offers valuable insights for the bioproduction industry. Graphical
研究领域:
生物化学
·
制药工程
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Hong Zhou,Bailin Cong,Yongqi Tian,Yu He,Huanghao Yang
Three new cyclic lipopeptides (compounds 1–3) were isolated from Bacillus sp. SY27F, which collected from the deep-sea hydrothermal vent in the Indian Ocean. The planar structures of compounds 1–3 were characterized by LC–MS, GC–MS and NMR, whereas their absolute configurations were determined by modified Marfey’s analysis and optical rotation contrast. They shared the same heptapeptide chain (N-L-Gln 1 -D-Leu 2 -L-Leu 3 -L-Val 4 -L-Asp 5 -D-Leu 6 -L-Ile 7 -C), differing only by the length of the fatty acid chain. Compounds 1–3 displayed significant cytotoxic activity against DU145, MCF-7 and HepG2 cancer cell lines with IC 50 values of 96.8–190.7 nM and moderate to weak cytotoxicity against A549, HeLa and HT1080, with IC 50 values of 9.3–43.2 μM, respectively. However, none of the three compounds showed any antibacterial activity against Escherichia coli, Staphylococcus aureus and Monilia albicans .
研究领域:
微生物学
·
药物化学
·
海洋生物学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 2.463
Xin-Jun Yu,Jie Sun,Ya-Qi Sun,Jian-Yong Zheng,Zhao Wang
In this study, the phytohormone gibberellin was evaluated for its effect on lipid and docosahexaenoic acid (DHA) accumulation in Aurantiochytrium sp.YLH70. It was found that 4 mg/mL of gibberellin increased biomass, lipid and DHA yield of Aurantiochytrium sp.YLH70 by 14.4%, 43.6% and 79.1%, respectively. Moreover, a GC–MS metabolomics method combined with a multivariate analysis was applied to reveal the metabolic mechanisms responsible for the increased lipid and DHA accumulation. The principal component analysis (PCA) revealed that metabolomics profiles from all groups were discriminated. 38 metabolites identified by the partial least-squares-discriminant analysis (PLS-DA) were responsible for responding to gibberellin treatment. Metabolic pathway analysis showed that gibberellin accelerated the rate of utilization of glucose, and metabolites in fatty acids biosynthesis and mevalonate pathway were increased, while metabolites in glycolysis and TCA cycle were decreased in Aurantiochytrium sp.YLH70. Moreover, the anti-stress mechanism in Aurantiochytrium sp.YLH70 might be induced by gibberellin.
研究领域:
生物化学
·
代谢组学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）