Scientific Reports
·
2024
·Vol. 14
·期 1
·1-12
·
IF 3.8
Wen Hong-jie,Zhu Shou-yan,Yang Hua-gang,Guo Feng-yong
Binding of Staphylococcus aureus protein A (SPA) to osteoblasts induces apoptosis and inhibits bone formation. Bone marrow-derived mesenchymal stem cells (BMSCs) have the ability to differentiate into bone, fat and cartilage. Therefore, it was important to analyze the molecular mechanism of SPA on osteogenic differentiation. We introduced transcript sequence data to screen out differentially expressed genes (DEGs) related to SPA-interfered BMSC. Protein–protein interaction (PPI) network of DEGs was established to screen biomarkers associated with SPA-interfered BMSC. Receiver operating characteristic (ROC) curve was plotted to evaluate the ability of biomarkers to discriminate between two groups of samples. Finally, we performed GSEA and regulatory analysis based on biomarkers. We identified 321 DEGs. Subsequently, 6 biomarkers ( Cenpf, Kntc1, Nek2, Asf1b, Troap and Kif14 ) were identified by hubba algorithm in PPI. ROC analysis showed that six biomarkers could clearly discriminate between normal differentiated and SPA-interfered BMSC. Moreover, we found that these biomarkers were mainly enriched in the pyrimidine metabolism pathway. We also constructed '71 circRNAs-14 miRNAs-5 mRNAs' and '10 lncRNAs-5 miRNAs-2 mRNAs' networks. Kntc1 and Asf1b genes were associated with rno-miR-3571. Nek2 and Asf1b genes were associated with rno-miR-497-5p. Finally, we found significantly lower expression of six biomarkers in the SPA-interfered group compared to the normal group by RT-qPCR. Overall, we obtained 6 biomarkers ( Cenpf, Kntc1, Nek2, Asf1b, Troap , and Kif14) related to SPA-interfered BMSC, which provided a theoretical basis to explore the key factors of SPA affecting osteogenic differentiation.
研究领域:
分子生物学
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细胞生物学
·
医学
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DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-13
·
IF 3.8
Zhu Dongsheng,Chen Feng,Qiang Hongjia,Qi Han
To clarify the impact of SETD2 on macrophage function in pediatric patients with acute suppurative osteomyelitis and to elucidate the precise underlying mechanism. To gain insights into the potential functions of SETD2, a comprehensive study was conducted utilizing a co-culture model of human bone mesenchymal stem cells (hBMSCs) and bone marrow-derived macrophages (THP-1). A range of techniques were employed, including quantitative polymerase chain reaction, western blotting, ELISA, alkaline phosphatase activity assays, alizarin red S staining, luciferase reporter gene assays, and chromatin immunoprecipitation, to unravel the intricate interactions and molecular mechanisms involving SETD2 in this system. It was observed that SETD2 expression was reduced in THP-1 cells stimulated by staphylococcal protein A (SPA). Furthermore, the downregulation of SETD2 resulted in elevated M1 macrophage polarization and glycolysis, effects that were mitigated by SPA stimulation. Notably, SPA-stimulated THP-1 cells exhibited an increase in HIF-1α expression, which exhibited an inverse correlation with SETD2 levels. Moreover, it was discovered that SETD2 functioned as a catalyst for H3K36me3 and bound to the HIF-1α gene, which, in turn, regulated HIF-1α expression. Furthermore, the suppression of HIF-1α abrogated the consequences of SETD2 downregulation on glycolysis and M1 macrophage polarization. Lastly, the study demonstrated that M1 macrophage polarization serves as a mediator for BMP4’s inhibitory effect on osteogenic differentiation of hBMSCs. This research has uncovered a previously unknown role of SETD2 in macrophages during osteomyelitis, revealing its significance in the pathogenesis of this condition. These findings suggest SETD2 as a novel target for the treatment of osteomyelitis.
研究领域:
免疫学
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分子生物学
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细胞生物学
·
骨科
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Chao Hou,Md All Amin Newton,Binjie Xin,Tingxiao Li
Wound infection caused by excessive exudate leads to overhydration of wounds and prevents wound healing. Thus, there is a need for a wound dressing to be developed that can unidirectionally export excess biological fluid while inhibiting bacterial infection. Herein, a nanofibrous bilayer membrane with a unidirectional water transport function was fabricated by electrospinning as a novel wound dressing, which can export excess biological fluid efficiently and further inhibit bacterial infection. The inner layer of the bilayer nanofiber membrane is polylactic acid (PLA)-zinc oxide (ZnO) nanofibers, which are hydrophobic and antibacterial. The outer layer is polyacrylonitrile (PAN)-sodium polyacrylate (SPA) nanofibers, which are hydrophilic and liquid-absorbent. The bilayer nanofibrous membrane's morphology, mechanics, thermal stability, wettability , water absorption, gas permeability, and unidirectional water transport ability were characterized and evaluated. The study showed that the nanofibrous bilayer membrane exhibited excellent physicochemical properties and biological performance, and its mechanical properties can reach 4.6 MPa. In addition, when the thickness of the hydrophobic layer is 3 µm and the thickness of the hydrophilic layer is 44 µm, the double-layer fiber membrane can effectively export the excess biological fluid in 5.09 s and prevent the wound from wetting again. The nanofibrous bilayer membrane with excellent water absorption, air permeability , thermal stability, and mechanical strength provides a feasible solution for chronic wound healing.
研究领域:
材料科学
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生物医学工程
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纳米技术
·
伤口护理
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产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2025
·
IF 10
Danyi Chen,Rongrong Zhao,Honglin Liu,Hailin Tian,Yiqun Tian,Di Huang,David Johnson,Yingping Huang
Developing efficient strategies for glyphosate (PMG) degradation while minimizing the formation of the toxic intermediate, aminomethylphosphonic acid (AMPA), is crucial for environmental remediation. In this study, two Schiff base metal complexes, Fe-SPA and Cu-SPA (SPA: salicylaldehyde o-phenylenediamine), were prepared and evaluated for their catalytic activity in activating H 2 O 2 under visible light irradiation (Vis, λ ≥ 420 nm) to degrade PMG. The Fe-SPA/H 2 O 2 (Vis) system achieved 99.9% degradation of 200 μmol/L PMG within 2 h, while the Cu-SPA/H 2 O 2 (Vis) system achieved complete degradation within 5 h. Interestingly, the Fe-SPA/H 2 O 2 (Vis) system selectively catalyzed PMG degradation via an environmentally friendly pathway, producing glycine as the final product. While in the Cu-SPA/H 2 O 2 (Vis) system, in addition to glycine, initially produced toxic AMPA, which was subsequently mineralized into non-toxic inorganic products. These differences in degradation pathways could be attributed to the preferential adsorption behavior of PMG and H 2 O 2 on the M-SPA complexes. Specifically, Fe-SPA selectively adsorbed the phosphate group of PMG, facilitating the C−P bond cleavage. Conversely, Cu-SPA preferentially adsorbed H 2 O 2 , resulting in a nonselective PMG degradation. Furthermore, hydroxyl radicals (·OH) and photogenerated holes (h⁺) were identified as the dominant reactive species in both systems. Fe-SPA exhibited excellent stability and maintained high catalytic performance across a wide pH range (3–10) and after five cycles of use. These findings highlighted the pivotal role of the central metal in determining pollutant degradation pathways. This study offers a promising strategy for efficient and environmentally safe PMG remediation.
研究领域:
环境科学
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催化化学
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产品关联
DOI
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Jie Han,Yao Liang,Chengxiu He,Yujia Tong,Weixing Li
A catalytic composite membrane was developed for the enhancement of esterification by lyophilization for the first time. The catalytic composite membrane was composed of a poly(vinyl alcohol) (PVA)-sodium alginate (SA) separation layer and a spongy porous catalytic layer cross-linked by PVA and 4-sulfophthalic acid (SPA). Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) results indicated the successful synthesis of the catalytic composite membrane. The membrane properties were evaluated by ethanol dehydration and esterification. The conversion rate of acetic acid reached 95.9% after 12 h. Compared with batch reactions, the conversion rate increased by 24.4%. After five cycles, the membrane still maintained outstanding catalytic activity. The resistance of mass transfer was analyzed, and the results showed that the porous structure reduced the catalytic layer resistance to total resistance from 70.27 to 32.88%. The composite membrane with a spongy porous catalytic layer exhibited superior dehydration and catalytic performance.
研究领域:
材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2018
·
IF 2.503
Qi Wang,Botao Wang
A sensitivity enhanced surface plasmon resonance (SPR) immunosensor based on Graphene oxide (GO) composite and staphylococcal protein A (SPA) co-modified photonic crystal fiber (PCF) is developed for human IgG detection. The PCF spliced between two section of multimode fibers (MMFs) is sputtered with gold film, which was then modified with graphene oxide and SPA for further immunosensing. Graphene oxide with abundant functional groups has a large surface area for more biomolecules immobilization and can enhance the interaction between Au film and external medium, leading to the sensitivity increased greatly. SPA can specifically bind the Fc region of the antibodies and has a high degree of orientation for capturing antibodies. Experimental results indicated that the refractive index (RI) sensitivity of graphene oxide modified Au-PCF SPR sensor reaches 4649.8 nm/RIU, which is about 1888 nm/RIU higher than that without graphene oxide film and the human IgG detection limit reaches as low as 10 ng/mL. Such a sensitivity enhanced immunosensor based on graphene oxide and SPA co-modified PCF shows a great promise in biosensing.
研究领域:
生物传感
·
免疫学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2015
·Vol. 96
·期 1
·331-338
·
IF 1.714
Juan Yang,Nana Wu,Jie Peng,Xiaoquan Yang,Jian Guo,Shouwei Yin,Jinmei Wang
BACKGROUND A novel soy protein aggregate enriched with isoflavones (SPA-IS), a mixture of soy protein and isoflavones (Mix), isoflavones (IS), and the soy protein were obtained to investigate the preventive effects on osteoporosis induced by retinoic acid in Kunming mice. RESULTS The serum osteocalcin levels in the Mix and SPA-IS groups decreased compared with the model group (mice showing retinoic acid-induced osteoporosis) ( P < 0.05). The trabecular analysis results showed an increased preventive effect of the SPA-IS group over the Mix group, the IS group, and the soy protein group. The results of both left tibial maximum load and the 4th lumbar structural strength differ between the IS and the SPA-IS groups. CONCLUSION The SPA-IS exhibited obvious oestrogenic activities on retinoic acid-induced osteoporosis in Kunming mice compared to Mix, IS, and soy protein. The results suggest that there is a potential use for SPA-IS in the treatment of osteoporosis induced by intake of retinoic acid. The improvement of bone indicators might be attributed to the formation of aggregate particles and the improvement of IS solubility. Copyright © 2015 Society of Chemical Industry
研究领域:
营养学
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药理学
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骨骼研究
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 5
·1630
·
IF 3.5
Qiang Xu,Huiting Yin,Mei Cui,Renliang Huang,Rongxin Su
A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect.
研究领域:
生物传感技术
·
材料科学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
Advanced Materials Technologies
·
2019
·Vol. 4
·期 5
·1900039
·
IF 5.395
Ce Zhang,Zhenfu Liu,Hongxia Liu,Shuai Wang,Yang Zhang,Chunhao Li,Yun Wu
The control of surrounding humidity by porous films is an interesting aspect for improving human comfort. Especially, light triggered humidity control is of relevance because light stimulus is contactless so that reducing the impact on both materials and surroundings. In this work, reversible light responsive films with tunable wettability and the ability of regulating surrounding humidity are developed from acrylated spiropyran (SPA)–methyl methacrylate (MMA) copolymer by electrospinning. The electrospun films for regulating wettability and surrounding humidity can be reversibly manipulated by the simple switch of UV and visible light irradiation because of the unique transformation between polar ring-opened status and nonpolar ring-closed status of spiropyran units. Morphology analyses and Cassie theory are used to explain this phenomenon and it is found that surface microstructure and effective chemical composition of the film surface can both significantly influence the reversible variation range of the value of surrounding humidity and surface wettability. The results show that the ability to regulate humidity of 40 wt% poly(SPA- co -MMA) film possessing bead-on-string fiber surface structure is the best.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
Sensor Letters
·
2016
·Vol. 14
·期 6
·623-629
·
IF 0
Li Liping,Liu Xiaojia,Xiao Qian,Zheng Yizhi,Wang Suiping,Feng Bo
A novel antibody immobilization method was developed for enzyme-linked immunosorbent assay (ELISA). The proposed method utilizes amine-bearing polymer macromolecules, namely, polyamidoamine (PAMAM) or polyethylenimine (PEI) to enhance antibody binding on polystyrene (PS) microwells,
as well as staphylococcal protein A (SPA) to realize orientation immobilization. After carboxylating the PS microwells with the solution of potassium permanganate (KMnO4) in diluted sulfuric acid (H2SO4), the PS surface was activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride and N-hydroxysuccinimide and then grafted with PAMAM or PEI to introduce free amino groups. Glutaraldehyde was subsequently added to conjugate the amino groups and SPA prior to antibody adsorption. X-ray photoelectron spectroscopy and atomic force microscopy were performed to
investigate the morphology of the PS surfaces before and after different treatments. The immunoglobulin binding sites were confirmed through anti-Fab and anti-Fc ELISA kits. Results from the alpha fetoprotein ELISA kit showed that the use of both PAMAM and SPA is the optimal method for antibody
immobilization. In this treatment, absorbance exhibited an evident gradient with antigen concentrations and reached higher values over 200 ng/mL antigen compared with the ones of other methods.
研究领域:
生物化学
·
分子生物学
·
分析化学
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DOI
APPLIED ORGANOMETALLIC CHEMISTRY
·
2025
·Vol. 39
·期 9
·e70351
·
IF 0
Xinliang Wang,Zhiwei Wang,Changqing Xia,Zhengshen Bao,Dehong Xu,Yinhai Ma,Shixing Wang,Weili Li
In this work, functional organic ligands are incorporated into the structurally stable metal–organic framework (UiO-66-SPA) to regulate the electrostatic potential and coordination positions of its inner and outer surfaces. The gold extraction efficiency of functionalized MOFs from acidic gold-containing wastewater is 2.65 times that of non-functionalized UiO-66-(SH) 2 . In this way, efficient recovery of Au(III) in complex trace Au(III) wastewater is achieved. After multiple regeneration cycles, UiO-66-SPA still maintains 99% of its adsorption capacity. Through characterization techniques such as X-ray photoelectron spectroscopy (XPS), density functional theory (DFT) calculations, kinetics, and thermodynamics, it is confirmed that the adsorption of Au(III) by UiO-66-SPA involves the synergistic effect of chelation coordination. This work provides a way to enhance adsorption for the post-modification of MOFs. The experiment of recovering Au(III) from industrial simulated wastewater improves the experimental data for the application of this material in Au(III) recovery. The research in this article is of great significance for MOFs materials in environmental protection, sustainable development of non-renewable resources, and green development of Au(III) resources.
研究领域:
环境科学
·
材料科学
·
无机化学
·
纳米技术
·
资源回收
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产品关联
DOI
SEPARATION SCIENCE AND TECHNOLOGY
·
2021
·
IF 2.475
Guichen Gong,Jie Liu,Yuexin Han
The structure–property relationship and adsorption mechanism of a novel collector for cassiterite, 2-carboxyethylphenylphosphinic acid (CEPPA), were comprehensively investigated and compared with those of styrene phosphonic acid (SPA) by density functional theory (DFT) calculations and flotation tests. The net Mulliken charge and frontier molecular orbital (FMO) theory study results indicated that CEPPA exhibited stronger electron-donating ability and higher chemical reactivity than SPA. The flotation test results demonstrated that CEPPA had stronger collecting ability for cassiterite in a much wider effective pH range than SPA. The adsorption simulation results confirmed that the CEPPA molecule was adsorbed on cassiterite (110) surface by two hydrogen bonds. CEPPA monoanion and dianion were adsorbed on cassiterite (110) surface by a series of O-Sn bonds, and the interactions between the O atoms in CEPPA phosphinic group with the Sn atoms on cassiterite (110) surface were stronger than those between the O atoms in CEPPA carboxyl group with the Sn atoms on cassiterite (110) surface. The formation of the O-Sn bonds was mainly contributed from the 2p orbital of O atoms and 5s and 5p orbitals of Sn atoms. The various adsorption configurations of CEPPA on cassiterite accounted for the better collecting ability of CEPPA for cassiterite than that of SPA. The quantitative analysis results were in good agreement with the qualitative experimental test results in a previous paper.
研究领域:
化学工程
·
材料科学
·
表面化学
·
矿物加工
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）