BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 39
·期 2
·e6067
·
IF 1.7
Jiale Liu,Rongfei Yuan,Qi Wang,Hai Qiao,Yuling Yang,Siyu Yang,Hua Zhang,Chang Chen
High-intensity focused ultrasound (HIFU) is a noninvasive soft tissue ablation technique, which utilizes ultrasound energy to induce thermal coagulation necrosis in targeted tissues. Whether this high energy causes side effects in vivo, such as the formation of peptide bonds, has not been fully investigated. Glycylglycine is the simplest dipeptide and hence is often used as a model compound for peptide studies. In this study, we developed and validated a sensitive quantification method based on ion-exchange solid-phase extraction, liquid chromatography, and tandem mass spectrometry (SPE-LC-MS/MS) for the analysis of glycylglycine without derivatization, and then used it to evaluate whether HIFU promoted peptide bond formation in aqueous solution (without enzymes) and plasma (with enzymes). The results showed that strong cation exchange SPE significantly reduced the matrix effect and improved the sensitivity of the LC-MS/MS method. No formation of glycylglycine in the aqueous solution or plasma was observed following HIFU irradiation.
研究领域:
生物化学
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2013
·
IF 2.487
Shian Zhong,Yanyue Kong,Ling Zhou,Chengyun Zhou,Xiaona Zhang,Yan Wang
In this study, we developed an efficient conversion process of dihydromyricetin to myricetin from Ampelopsis grossedentata extracts. The content of myricetin increased from 2.38% to 85.57%, demonstrating the successful dehydrogenation of dihydromyricetin. Molecularly imprinted polymers (MIPs) were prepared by surface imprinting method using silica microspheres as the support matrices and myricetin as template. The MIPs were applied for the selective adsorption of myricetin. The chemical structure of the MIPs was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Static, dynamic and selective adsorption experiments showed that the MIPs exhibited good adsorption ability, rather fast template rebinding kinetics, and appreciate selectivity over structurally related compounds. Accordingly, the MIPs were applied as the selective sorbent in SPE to purify myricetin obtained through dehydrogenation, followed by HPLC-UV analysis. The recoveries of myricetin and dihydromyricetin were 92.7% and 55.6%, respectively. This study demonstrates the feasibility of using the developed MIP-SPE method to purify and enrich myricetin in the natural products.
研究领域:
药学
·
分析化学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Weikang Shen,Haobo Wang,Zhouzhe Lan,Guangzhi Xu,Qinxue Ni,Qiufen Mo,Youzuo Zhang,Yan Wang
Gardenia fruit oil (GFO) is a new emerging edible woody oil with few investigations of its’ refinement process. In this study, different degumming, deacidification and bleaching technologies were introduced for realizing moderate refining of the GFO based on the preservation of bioactive micronutrients. Firstly, a simple multi-stage solid phase extraction (SPE) combined with a lab- affordable HPLC-UV instrument was developed for determinations of several vital biological constituents, such as crocetin, geniposide, polyphenols, phytosterols and squalene in the GFO. Satisfactory limit of detection (LOD), limit of quantification (LOQ), recovery, intra-day and inter-day precision were obtained. Subsequently, changes of those phytochemicals during three types of degumming, followed by three kinds of deacidification and then three ways of bleaching were investigated, offering insightful suggestions for achieving moderate refining of the GFO. Finally, the refined GFO might be suggested as a potential functional edible oil with valuable micronutrients. To our knowledge, this is the first study investigating moderate refinement techniques for GFO, as well as developing novel methods for phytonutrients analysis in the GFO.
研究领域:
食品科学
·
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Mengke Jin,Jiajia Shan,Xue Wang,Tao Ren,Xinjing Li
Florfenicol has been widely used in livestock and aquaculture to treat bacterial infections. Due to the abuse of antibiotics, florfenicol has been released into the environment. Thus, there is a need for the rapid determination of trace levels of florfenicol in the environment. To improve the sensitivity for florfenicol, solid phase extraction (SPE) was employed to enrich florfenicol from multi-residue antibiotic mixtures followed by in situ determination by surface-enhanced Raman spectroscopy (SERS). Activated carbon, C18, graphene oxide (GO), and sand were applied to concentrate florfenicol in water. Silver nanoparticles (AgNP)-coated silicon (AgNPs@Si) substrate was employed to determine florfenicol enriched on the adsorbent. The results showed that the detection limit of florfenicol in water was 2.8 × 10−9 mol/L following the adsorption and enrichment of activated carbon. Besides, activated carbon was performed to extract florfenicol from the multi-residue antibiotic mixtures, including enrofloxacin, norfloxacin, and florfenicol. The results showed that activated carbon combined with SERS specifically determined florfenicol in complex antibiotic mixtures with a detection limit of 2.8 × 10−9 mol/L. Moreover, to better understand the adsorption mechanisms, the mechanism of the interaction of florfenicol with activated carbon was investigated.
研究领域:
环境科学
·
分析化学
·
药物分析
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Xiao Yu,Wanxiang Yu,Xiaohui Zhang,Yongjie Wang,Shumei Wang,Haiyun Zhai
Based on advantages of capillary electrophoresis (CE), a new solid-phase extraction (SPE) coupled with CE has been developed for preconcentration , enrichment and determination of anthraquinones and flavonoids (rutin, emodin , quercetin , 1,8-dihydroxyanthraquinone) in honey. The environmental-friendly chitin activated after an easy processing is selected as the adsorbent to enrich analytes. Then, chitin was filled into the filter as the solid phase. To improve the extraction effect, some key parameters of extraction were optimized. Under the optional extraction conditions, the chitin showed excellent adsorption capacity and selectivity over rutin , emodin, quercetin, and 1,8-dihydroxyanthraquinone, with enrichment factors reaching 5 folds. The CE coupled with fluorescence detection was used for the detection. Results prove the method is simple, fast, and highly sensitive, with the limit of detection (LOD) is 3.00–200.0 ng/mL; the recovery is 90.0–107.0%, and relative standard deviation of (RSD) is 1.8–8.3%.
研究领域:
分析化学
·
食品科学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Chujun Liu,Xing Xie,Yawen Guo,Bo Wang,Kaizhou Xie,Yuhao Dong,Chenggen Yang,Zhixin Feng,Wenbin Bao
A novel, simple and high-sensitivity gas chromatography-tandem mass spectrometry (GC-MS/MS) method combined with external calibration was developed for the qualitative and quantitative determination of penicillin G residues in poultry tissues and pork. Samples were extracted by an ASE 350 apparatus with 0.2 mol/L phosphate buffer solution (pH 8.0) and purified on HLB solid-phase extraction (SPE) cartridges (60 mg/3 mL). Trimethylsilyl diazomethane (TMSD) was selected as the derivatization reagent for precolumn derivatization. The recoveries of analytes at the limit of quantitation (LOQ) and 25, 50 and 100 μg/kg ranged from 80.67% to 96.18%, with RSDs below 3.17%. The determination coefficient (R 2 ) values were above 0.9993 in the corresponding concentration range. The limits of detection (LODs) (S/N ≥ 3) and LOQs (S/N ≥ 10) ranged from 1.50 to 4.10 μg/kg and 4.50–8.20 μg/kg, respectively. In addition, the newly established method was implemented to analyze poultry tissues and pork purchased from a local market, demonstrating the sensitivity and applicability of the method.
研究领域:
分析化学
·
食品安全
·
兽医药理学
✓ 已验证
DOI
高影响因子
Emerging Contaminants
·
2023
·
IF 6
Xue Cheng,Ning Fang,Yuxi Ge,Wenchao Li,Yamei Hui,Zilong Tang,Yu Wan,Yuancheng Li
It is essential to determine the occurrence of emerging organic contaminants (EOCs), such as pharmaceuticals and personal care products (PPCPs), in the ambient environment to address growing public concerns. However, such analysis is quite challenging due to the low trace level of such contaminants in water, which therefore requires several litres of water samples. In this study, a large volume solid phase extraction (LV-SPE) device was developed and evaluated for its performance in monitoring PPCPs as an example. Relatively good recoveries and reproducibility were obtained under specific operating conditions: a water sample volume of no more than 20 L, a flow rate not exceeding 120 mL/min, and a methanol elution volume of at least 30 mL. In addition, the results from the onsite enrichment approach using LV-SPE were compared with those from the conventional approach using a standard SPE device in the laboratory for real groundwater samples. Among the eight selected PPCPs (nalidixic acid, carbamazepine, bezafibrate, clofibric acid, sulfadiazine, sulfamethoxypyridazine, sulfamethazine and sulfamonomethoxine), LV-SPE approach detected more target compounds. While the detected concentrations were generally comparable, slightly higher concentrations were observed for carbamazepine, clofibric acid, sulfadiazine and sulfamethazine using the LV-SPE method. The developed LV-SPE device provides an alternative approach for trace analysis of PPCPs and may also be applicable for other emerging organic contaminants.
研究领域:
环境科学
·
分析化学
·
水质监测
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Xuhui Li,Gengjian Meng,Zhuobin Chang,Xinxin Lian,Jianhua Ma,Ruichao Guo,Yulong Wang
The freely dissolved concentrations (FDCs) of pollutants are related to their bioavailability in the environment, and the diffusive gradients in thin films technique (DGT) can obtain the FDC of an analyte. Aiming for the detection of the FDCs of tetracyclines (TCs), we used a polyacrylamide hydrogel comprising acrylamide and acrylaide agarose cross-linker as diffusive and binding gels, and a commercial solid-phase extraction (SPE) packing, namely polymer sorbent (PLS), as an adsorption material in the binding gel for the preparation of the organic-diffusive gradients in thin films (o-DGT) devices. The results showed that the diffusion coefficients of tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC) in the diffusive gels were 1.08 × 10 −6 , 1.08 × 10 −6 and 1.03 × 10 −6 cm 2 / s at 25 °C, respectively. The binding gel showed excellent performance with adsorption capacities of 534.88–569.42 µg/disc for TC, 527.18–565.98 µg/disc for OTC and 1320.12–1320.86 µg/disc for CTC, respectively. The uptake efficiencies were 94.21–111.12, 71.25–88.44 and 76.10–86.62% for TC, OTC and CTC, respectively, with the TCs concentration of 0.05–10 mg/L. The adsorption kinetics of TCs could be described with a pseudo-second-order model (POSM, R 2 >0.97). According to the result of adsorption kinetics, the adsorption rate of TCs in the binding gel was not as fast as that of heavy metals, suggesting that the TCs concentrations at the boundary of binding gels in the o-DGT devices could not decrease to zero. After correction of the boundary concentration, the FDCs accounted for 30.30–56.90, 48.10–64.68 and 16.55–50.16% for TC, OTC and CTC, respectively, while their concentrations ranged from 0.2 to 10.0 mg/L. Our results suggested that SPE packing might be an ideal adsorption material for o-DGT binding gels, and that adsorption kinetics should be corrected when calculating the FDCs of organic pollutants.
研究领域:
环境科学
·
分析化学
·
污染物监测
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ting Zhang,Danni Ma,Fuman Cai
The accurate detection of glucose is critical for clinical diagnostics and metabolic monitoring. This study focused on the successful synthesis and incorporation of the glucose dehydrogenase (GDH) gene sequence from Burkholderia cepacia into a plasmid, followed by codon optimization and modification of cysteine motifs for increased expression in E. coli . The engineered E. coli strain demonstrated efficient heterologous expression of stable GDH. Remarkably, the recombinant strain not only enabled cost-effective GDH production but also exhibited excellent glucose catalytic activity, with a detection range of 1 μM to 10 mM and a minimum detectable concentration of 1 μM. This functionality makes it suitable for applications of AuNPs/SPE/glucose biosensors, offering high sensitivity for glucose detection—a novel achievement not previously reported. These findings have the potential to advance the clinical utilization of AuNPs/tetracysteine/GDH-modified biosensors and may promote the development of GDH-based electrochemical biosensors.
研究领域:
生物化学
·
生物技术
·
分析化学
·
分子生物学
·
纳米技术
·
临床诊断
·
代谢监测
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Mengyue Gao,Qiao Chen,ZhiHao Li,YiFang Zhan,LiHua Wang,Ting He,Qi Yao,Fengmei Jin,Jiming Hu
A SPE-SERS method was developed for the detection of several antibiotic residues in dairy products. Gold nanoparticles (Au NPs) encapsulated with an ultrathin Cr-MIL-101 shell (Au@Cr-MIL-101 NPs) have been synthesized, and the thickness of Cr-MIL-101 shell can be precisely controlled to 3 nm. As a superior solid phase extraction (SPE) adsorbent, Cr-MIL-101 acts as a shell layer to effectively enrich antibiotics within the localized surface plasmon resonance (LSPR) field of Au NPs, which enhances the SERS signal and eliminates background interference. The method can achieve highly sensitive and high-throughput detection for tetracycline hydrochloride, sulfapyridine and benzylpenicillin sodium in dairy products, and the detection limits (LOD) are as low as 2.237, 2.644 and 4.662 ppb respectively. The recoveries of antibiotic residues in spiked dairy products ranged from 72.31 % to 146.7 % with matrix effects (ME) of −15.13 % to 28.68 %. Thus, this method holds significant promise for rapid detection of antibiotics in milk.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 136
·期 22
·4774-4779
·
IF 3.913
Di Pan,Chunyan Chen,Fei Yang,Yiming Long,Qingyun Cai,Shouzhuo Yao
Titanium wire-based solid phase extraction (SPE) is developed for the analysis of six different polycyclic aromatic hydrocarbons in water samples with coupling with high performance liquid chromatography. The adsorbent is a Ti wire on which TiO2 nanotubes are first formed by anodization and then Au nanoparticles and n-octadecanethiol are modified. The surface composition and structure of the adsorbent were characterized in detail using field emission scanning electron microscope and Fourier transform infrared spectroscopy. The main parameters affecting the extraction efficiency, such as the volume of the organic modifier, extraction time, elution solvent, and desorption time were optimized. Good linearities ranging from 0.025 to 30 μg L−1 with correlation coefficients (R2) ranging from 0.9964 to 0.9999 and low limits of detection were obtained in spiked water samples. Furthermore, this method was successfully applied in the analysis of real river water samples with recoveries in the range of 75.33–115.83% and relative standard deviations ranging from 4.85 to 10.77%. The developed SPE can be a particularly convenient means of sample pretreatment for environmental water samples.
研究领域:
环境科学
·
分析化学
·
水质分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 82
·期 8
·1285-1293
·
IF 1.401
Wang Huimin,Guo Bin,Pang Xiaoya,Yu Huan,Liu Haiyan,Yan Hongyuan,Han Dandan,Guo Huaizhong,Bai Ligai
A poly(NMA-ST- co -TAIC-EDMA) monolithic column was prepared by redox initiation method using N -methylolacrylamide (NMA) and styrene (ST) as co-monomers. The composite monolith was characterized by scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherm and was used as a solid phase extraction (SPE) absorbent for enrichment of β -sitosterol by high performance liquid chromatography (HPLC). Under the optimum conditions for extraction and determination, the calibration equation was y = 1.02247 x + 0.1766; the linear range was 0.015–0.75 mg mL −1 and the linear regression coefficient was 0.998. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.006 mg mL −1 and 0.02 mg mL −1 , respectively. The spiked recoveries of β -sitosterol in edible oil samples were 83.60–103.88%; precisions for intra-day and inter-day assays presented as relative standard deviations were less than 6.0% and 6.2%, respectively. The enrichment factor of β -sitosterol was 60 and the results showed that the monolithic column had high selectivity and good permeability as an on-line SPE absorbent for the enrichment and determination of β -sitosterol from edible oils.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）