ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Sun Rengang,Zhou Yadong,Fang Yunzhu,Qin Yirui,Zheng Yekai,Jiang Li
The harm and impact of the COVID-19 pandemic have highlighted the importance of fast, sensitive, and cost-effective virus detection methods. In this study, we developed a DNA aptamer sensor using nanoparticle-enhanced surface plasmon resonance imaging (SPRi) technology to achieve efficient labeling-free detection of SARS-CoV-2 S protein. We used the same DNA aptamer to modify the surface of the SPRi sensor chip and gold nanoparticles (AuNPs), respectively, for capturing target analytes and amplifying signals, achieving ideal results while greatly reducing costs and simplifying the preparation process. The SPRi sensing method exhibits a good linear relationship ( R 2 = 0.9926) in the concentration range of 1–20 nM before adding AuNPs to amplify the signal, with a limit of detection (LOD) of 0.32 nM. After amplifying the signal, there is a good linear relationship ( R 2 = 0.9829) between the concentration range of 25–1000 pM, with a LOD of 5.99 pM. The simulation results also verified the effectiveness of AuNPs in improving SPRi signal response. The SPRi sensor has the advantage of short detection time and can complete the detection within 10 min. In addition, the specificity and repeatability of this method can achieve excellent results. This is the first study to simultaneously capture a viral marker protein and amplify the signal using polyadenylic acid (polyA)–modified DNA aptamers on the SPR platform. This scheme can be used as a fast and inexpensive detection method for diagnosis at the point of care (POC) to combat current and future epidemics caused by the virus.
研究领域:
生物传感器
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病毒学
·
纳米技术
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Shaopeng Li
The label-free and sensitive detection of small molecule drugs on SPRi is still a challenging task, mainly due to the limited surface immobilization capacity of the sensor. In this research, a dextran hydrogel-coated gold sensor chip for SPRi was successfully fabricated via photo-cross-linking for enhanced surface immobilization capacity. The density of the dextran hydrogel was optimized for protein immobilization and sensitive small molecule detection. The protein immobilization capacity of the hydrogel was 10 times greater than a bare gold surface, and 20 times greater than an 11-mercaptoundecanoic acid (MUA) surface. Such a drastic improvement in immobilization capacity allowed the SPRi sensor to detect adequate response signals when probing small molecule binding events. The binding signal of 4 nM liquid-phase biotin to streptavidin immobilized on the dextran surface reached 435 RU, while no response was observed on bare gold or MUA surfaces. The dextran hydrogel-coated SPRi sensor was also applied in a kinetic study of the binding between an immunosuppressive drug (FK506) and its target protein (FKBP12) in a high-throughput microarray format. The measured binding affinity was shown to be consistent with reported literature values, and a detection limit of 0.5 nM was achieved.
研究领域:
分析化学
·
生物传感器
·
药物发现
·
生物物理学
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产品关联
DOI
高影响因子
Coatings
·
2023
·Vol. 13
·期 8
·1400
·
IF 3.4
Yuyan Peng,Li Jiang,Yifan Li,Xiaoping Yu
Soluble programmed death-ligand 1 (sPD-L1) levels vary widely among different stages of tumor development, so the direct quantification of sPD-L1 as a cancer biomarker is useful in cancer diagnosis, prognosis and therapeutic assessment. There is an urgent need for an sPD-L1 detection method with a broad detection range and high sensitivity for monitoring cancer progression and evaluating the effectiveness of immunotherapy in real time. Herein, we have reported an enzyme-free, label-free surface plasmon resonance imaging (SPRi) sensor based on an aptamer/sPD-L1/anti–PD-L1 sandwich structure with gold nanoparticle (AuNP) signal enhancement for the ultrasensitive quantitative measurement of sPD-L1 for the first time. The gold chip of the SPRi sensing platform was modified by DNA aptamers, sPD-L1 was specifically adsorbed on the surface of a DNA aptamer-modified gold chip and then coupled with anti–PD-L1. Thus, the detection of sPD-L1 at different concentrations was realized through the formation of an aptamer/sPD-L1/anti–PD-L1 sandwich structure. We also enhanced the SPR signal via AuNPs to further improve sensor sensitivity. The SPRi sensor is able to measure sPD-L1 within a linear range of 50 pM–10 nM and 100 fM–50 pM, and the minimum detection limit is 19 fM. The sensor is designed to be widely applicable, with better accuracy and reliability for more application scenarios. The prepared SPRi sensor shows great potential in improving the sensitivity of detecting sPD-L1. The proposed method demonstrates the excellent performance of the SPRi sensor and provides a possibility for the establishment of effective clinical assay methods in the future.Keywords:programmed death-ligand 1;surface sandwich assay;DNA aptamer/antibody bioreceptor pair;surface plasmon resonance;tumor biomarker
研究领域:
癌症生物学
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生物传感器
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纳米技术
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免疫学
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分析化学
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产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 15
·5041
·
IF 4.927
Weiguo Wang,Minkang Feng,Xiaomeng Li,Feiyu Chen,Zhihao Zhang,Wenlong Yang,Chen Shao,Liming Tao,Yang Zhang
Actinomycetes play a vital role as one of the most important natural resources for both pharmaceutical and agricultural applications. The actinomycete strain SPRI-371, isolated from soil collected in Jiangsu province, China, was classified asStreptomyces aureusbased on its morphological, physiological, biochemical and molecular biological characteristics. Its bacterial activity metabolites were identified as aureonuclemycin (ANM), belonging to adenosine derivatives with the molecular formula C16H19N5O9for ANM A and C10H13N5O3for ANM B. Simultaneously, the industrial fermentation process of a mutatedS. aureusstrain SPRI-371 was optimized in a 20 m3fermentation tank, featuring a rotation speed of 170 rpm, a pressure of 0.05 MPa, an inoculum age of 36–40 h and a dissolved oxygen level maintained at 1–30% within 40–80 h and at >60% in the later period, resulting in an ANM yield of >3700 mg/L. In the industrial separation of fermentation broth, the sulfuric acid solution was selected to adjust pH and 4# resin was used for adsorption. Then, it was resolved with 20% ethanol solution and concentrated in a vacuum (60–65 °C), with excellent results. Antibacterial experiments showed that ANM was less active or inactive againstXanthomonas oryzaepv.oryzae,Xanthomonas citrisubsp.citriandXanthomonas oryzaepv.oryzicolaand most bacteria, yeast and fungi in vitro. However, in vivo experiments showed that ANM exhibited extremely significant protective and therapeutic activity against diseases caused byX. oryzaepv.oryzaeandX. oryzaepv.oryzicolain rice andX. citriin oranges and lemons. In field trials, ANM A 150 gai/ha + ANM B 75 gai/ha exhibited excellent therapeutic activity against rice bacterial leaf blight, citrus canker and rice bacterial leaf streak. Furthermore, as the dosage and production cost of ANM are lower than those of commercial drugs, it has good application prospects.Keywords:aureonuclemycin;Xanthomonas oryzaepv.oryzae;Xanthomonas citrisubsp.citri;Xanthomonas oryzaepv.oryzicola;antibacterial activity
研究领域:
药学
·
农学
·
微生物学
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DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2015
·
IF 3.725
Vikramjeet Singh,Amita Nand,Sarita,Jiwen Zhang,Jingsong Zhu
Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), α-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI−TOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this comparative study will surely help the researchers to further upgrade these surfaces and especially SIP to make it more universal for biosensor microarray applications including biomarker discovery in high throughput format.
研究领域:
生物技术
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医学诊断
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2026
·
IF 6.7
Youjun Zeng,Hao Du,Jun Yang,Chenglong Guo,Yufeng Yuan,Jie Zhou
Blood-based detection of Tau presents a promising strategy for early screening of Alzheimer's disease (AD). This study introduces a novel surface plasmon resonance imaging (SPRi) platform that employs an array of low-cost monochromatic LEDs as excitation light sources, effectively replacing conventional spectrometer-based systems to achieve cost reduction and enhanced operational stability. We developed an adaptive fitting algorithm that automatically identifies optimal spectral bands for analysis, enabling high-precision SPR measurements across an extended dynamic range. The system demonstrates exceptional performance with a RI resolution of 1.96 × 10 −6 RIU and a dynamic range of 0.0136 RIU. To further enhance sensitivity, we implemented a signal amplification strategy based on localized sample heating, which promotes molecular enrichment at both the center and periphery of evaporating droplets. This approach increases local target concentrations and enhances interaction probability with surface-immobilized capture probes. The label-free amplification method eliminates the requirement for secondary signal enhancement, substantially simplifying the detection workflow. Using this integrated approach, we achieved ultrasensitive Tau detection with a limit of detection (LOD) of 437 fg/mL (7.95 fM) and established a linear detection range spanning from 1 pg/mL to 10 ng/mL. This technique provides a robust platform for AD-related Tau quantification and demonstrates significant potential for facilitating early AD diagnosis.
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 13
·3134-3140
·
IF 3.864
Xiaotong Wei,Xiaolei Duan,Xiaoyan Zhou,Jiangling Wu,Hongbing Xu,Xun Min,Shijia Ding
Herein, a dual channel surface plasmon resonance imaging (SPRi) biosensor has been developed for the simultaneous and highly sensitive detection of multiplex miRNAs based on strand displacement amplification (SDA) and DNA-functionalized AuNP signal enhancement. In the presence of target miRNAs (miR-21 or miR-192), the miRNAs could specifically hybridize with the corresponding hairpin probes (H) and initiate the SDA, resulting in massive triggers. Subsequently, the two parts of the released triggers could hybridize with capture probes (CP) and DNA-functionalized AuNPs, assembling DNA sandwiches with great mass on the chip surface. A significantly amplified SPR signal readout was achieved. This established biosensing method was capable of simultaneously detecting multiplex miRNAs with a limit of detection down to 0.15 pM for miR-21 and 0.22 pM for miR-192. This method exhibited good specificity and acceptable reproducibility. Moreover, the developed method was applied to the determination of target miRNAs in a complex matrix. Thus, this developed SPRi biosensing method may present a potential alternative tool for miRNA detection in biomedical research and clinical diagnosis.
研究领域:
生物传感技术
·
分子诊断
·
分析化学
·
医学检测
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DOI
PubMed
高影响因子
RSC Advances
·
2014
·
IF 3.708
Vikramjeet Singh,Jinsong Zhu,Amita Nand,Zhiqiang Cheng,Yang Mo
Here we report the 3D photo-cross-linking strategy based on the use of α-cyclodextrin as an alternative surface chemistry for the preparation of small molecule microarray (SMMs). Surface plasmon resonance imaging (SPRi) was used in combination with SMMs as a detection technology. Small molecules of diverse structure containing different functional groups can be covalently captured on a single slide and displayed in various orientations in a given spot. Several known small molecules-protein complexes were selected to validate this platform. Furthermore, a comparison between 2D PEG and 3D α-cyclodextrin photo-cross-linking surface was performed using fluorescence and SPRi techniques. Data produced from both type of surfaces strongly favors the better performance of 3D over 2D surface in terms of loading capacity, spot morphology, signal response, and kinetics values. We believe that this strategy will expand the utility of small molecule microarray for high throughput screening against a variety of target proteins.
研究领域:
分析化学
·
分子生物学
·
生物化学
·
药物发现
✓ 已验证
产品关联
DOI
高影响因子
Green Processing and Synthesis
·
2024
·Vol. 13
·期 1
·
IF 3.8
Jianuo Sun,Haokun Ke,Jinghan Wang,Xianchao Du,Hongxia Hao,Hong Zhou
Based on the surface plasmon resonance imaging (SPRi) instrument, we established a new method of analyzing morphine in urine by processing a calibration curve. According to an inhibition immunoassay, gradient concentration of morphine and morphine-BSA fixed on the chip competitively combine with morphine antibody on the chip. Given the three mathematical models, the data of SPRi signals generated from SPRi with morphine was processed to obtain the calibration curve. Ultrafiltration was used to pretreat blank urine samples with adding morphine, and then investigated the advantages and disadvantages of each model. With a limit detection of 6.57 ng·mL −1 , the method and mathematical models can provide robust support for SPRi sensors used in further environmental detection, such as the epidemiological study of sewage.
研究领域:
分析化学
·
生物化学
·
环境科学
·
传感器与执行器
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2015
·
IF 3.725
Vikramjeet Singh,Kuldeep Singh,Amita Nand,Huanqin Dai,Jianguo Wang,Lixin Zhang,Alejandro Merino,Jingsong Zhu
In order to increase the scope and utility of small molecule microarrays (SMMs) we have combined SMMs and SPRi to screen small molecule antagonists against protein targets. Several small molecules, including immunosuppressive drugs (rapamycin and FK506) and reported inhibitors (FOBISIN and Blapsin) of 14-3-3ζ proteins have been used to validate this technology. Furthermore, a small library of isatin derivatives have been synthesized and screened on developed platform against 14-3-3ζ protein. Three molecules, derived from the endogenous intermediate isatin termed, FZIB-35, FZIB-36 and FZIB-38 were identified as novel inhibitors which shows significant interaction with 14-3-3ζ. A mutation in the binding groove of 14-3-3ζ, (K49E), almost abolishes the binding of these compounds to 14-3-3ζ protein. To exclude the probability of false positives, two more purified proteins (PtpA and BirA) were also tested. Furthermore, in order to confirm the binding pocket specificity, competition assay against R18 peptide was also carried out on presented platform. We show that SMMs in combination with SPRi are a powerful method to identify lead compounds in high throughput manner without the need to develop an activity based assay.
研究领域:
化学
·
生物化学
·
分子生物学
·
药物发现
✓ 已验证
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2014
·
IF 2.305
Deependra Tyagi,Javier Batista Perez,Amita Nand,Cheng Zhiqiang,Peizhe Wang,Jie Na,Jingsong Zhu
Surface plasmon resonance imaging (SPRi) has emerged as a versatile biosensor to detect a wide range of biomolecular interactions with divergent potential applications. However, the use of this advanced-level technology for stem cell lysate study is still not much explored. Cell lysates are significant biological analytes used for disease diagnostics and proteomic studies, but their complex nature limits their use as an analyte for SPRi biosensors. Here, we review the problems associated with the use of SPRi for stem cell lysate study and examine the role of surface chemistry, running buffer, and blocking solution in order to minimize nonspecific adsorption (NSA). We detect the expression of Oct4, Sox2, Nanog , Rex1 , and Lin28 biomarkers present in mouse embryonic stem cell (mESC) lysate against their corresponding antibodies immobilized on the sensor surface with reduced NSA. The current study shows that the conjunction of SPRi and microarray can be used as a label-free, high-throughput, and rapid technique for detection of biomarkers and their relative abundance in stem cell lysate study.
研究领域:
生物传感
·
干细胞生物学
·
蛋白质组学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jie Zhou,Xueliang Wang,Jiajie Chen,Youjun Zeng,Dayong Gu,Bruce Zhi Gao,Yonghong Shao
Occult blood testing is a routine screening method for evaluating the function of the urinary system and digestive tract. The hemoglobin (Hb) content in human excreta is an important indicator of the presence of occult blood. In this study, a surface plasmon resonance imaging (SPRi)-based immunosensor with high sensitivity was developed for the detection of Hb. The immunosensor was constructed by physically immobilizing anti-Hb antibodies on the detection channels. Samples for evaluation were pre-incubated with the polymeric microsphere-antibody conjugate. The shift in resonance wavelength induced by the specific binding of microsphere-antibody-antigen and immobilized antibodies was monitored in real time. Owing to the amplification effect of microspheres, the detection limit of the immunosensor was reduced 42-fold to 4.8 ng/mL under the optimized concentration of immobilized antibody (10 μg/mL) with microsphere-antibody conjugate dilution ratio of 1:200 and incubation time of the microsphere-antibody conjugate and samples (10 min). The immunosensor displayed a good linear response to Hb ranging from 10 ng/mL to 500 ng/mL in both test solutions and in 1:30 urine sample, as well as a good specificity toward Hb and an acceptable recovery rate for spiked Hb solution in urine sample. The immunosensor was also exploited to detect the Hb concentration in 50 clinical urine samples. Therefore, the proposed SPRi immunosensor developed in this study provides a simple, rapid, high-throughput, and sensitive immunology strategy for the detection of occult blood.
研究领域:
生物传感器
·
分析化学
·
医学诊断
·
生物医学工程
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产品关联
DOI
高影响因子
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