NUCLEIC ACIDS RESEARCH
·
2022
·Vol. 50
·期 5
·2973-2985
·
IF 19.16
Ba Fang,Liu Yushi,Liu Wan-Qiu,Tian Xintong,Li Jian
Serine integrases are emerging as one of the most powerful biological tools for synthetic biology. They have been widely used across genome engineering and genetic circuit design. However, developing serine integrase-based tools for directly/precisely manipulating synthetic biobricks is still missing. Here, we report SYMBIOSIS, a versatile method that can robustly manipulate DNA parts in vivo and in vitro. First, we propose a ‘keys match locks’ model to demonstrate that three orthogonal serine integrases are able to irreversibly and stably switch on seven synthetic biobricks with high accuracy in vivo. Then, we demonstrate that purified integrases can facilitate the assembly of ‘donor’ and ‘acceptor’ plasmids in vitro to construct composite plasmids. Finally, we use SYMBIOSIS to assemble different chromoprotein genes and create novel colored Escherichia coli. We anticipate that our SYMBIOSIS strategy will accelerate synthetic biobrick manipulation, genetic circuit design and multiple plasmid assembly for synthetic biology with broad potential applications.
研究领域:
合成生物学
·
基因工程
·
分子生物学
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DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2022
·Vol. 11
·期 17
·2102724
·
IF 11.092
Hui Xiong,Xiaoyan Liu,Zuohan Xie,Linyuan Zhu,Haipeng Lu,Cheng Wang,Jing Yao
The clinical anti-vascular endothelial growth factor (anti-VEGF) drugs and metronomic chemotherapy (MET) induced tumor vascular normalization treatment (TVNT) are easily antagonized by tumor microenvironment metabolic cross-talk between tumor cells and endothelial cells (ECs). To overcome this dilemma, nanodrug with the ability of ECs targeted glycolysis inhibition and nanodrug with the ability of tumor cell glycolysis inhibition, anti-VEGF, and MET are combined to prepare Nano-combination the pathways related to angiogenesis, tumor cell proliferation, and immunosuppression and breaking the negative sugar-lipid-protein metabolism balance in tumor microenvironment. Thus, stronger and more lasting normalized tumor vascular network and remarkable antitumor efficacy are obtained after treatment, constructing a positive feedback loop between TVNT and anti-tumor therapy. Above all, this study provides a new insight for solving the bottleneck of clinical TVNT.
研究领域:
肿瘤学
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药理学
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纳米技术
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癌症生物学
·
血管生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Cong-Cong Tang,Tian-Yang Wang,Rong Wang,Cristiano Varrone,Zixuan Gan,Zhang-Wei He,Zhi-Hua Li,Xiaochang C. Wang
Triclosan (TCS) is an antimicrobial agent and frequently detected in wastewater or water body. This study investigated the role of TCS in microalgal-bacterial symbiosis (MABS) system treating wastewater. The results showed that the removal efficiencies of NH 4 + –N, total nitrogen, and total phosphorus decreased under increased TCS stress, with decrease ratios of 26.5%, 16.9%, and 34.7%. The activities of microalgae were more affected than that of bacteria. The secretion of extracellular polymeric substances (EPSs) and activity of superoxide dismutase firstly increased and then decreased with aggravated TCS stress, while the accumulation of malondialdehyde increased, leading to increased permeability of cytomembrane and bioaccumulation of TCS. In addition, the aggregation properties of microalgae and bacteria were enhanced with TCS loading increasing, and the migration of TCS was affected by enhanced EPSs secretions and MABS aggregates. This work may provide some new insights into the roles of TCS in MABS system.
研究领域:
环境科学
·
微生物学
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PubMed
高影响因子
Nanoscale
·
2026
·
IF 5.2
Zhenxi Xie,Guiling Zeng,Mingtao Zheng,Haoran Zhang,Xiaoqin Pan,Wei Li,Bingfu Lei
Diazotrophs-plant symbiosis model system has been developed, but the nitrogen-fixation efficiency of engineered systems remains suboptimal compared with wild-type counterparts. In this study, carbon dots (CDs) with benzoquinone and phenazine structures, prepared from o-phenylenediamine (o-PD) and catechol (CAT), were selected as an electron donor and an electron relay to construct a CDs/microalgae hybrid nitrogen-fixation system. The photocurrent response confirmed the photoelectron-donor capability of the CDs. Nostoc commune Vauch was chosen as the diazotroph. The hybrid system produced 1.32-fold more ethylene than pure microalgae. When integrated with a lettuce hydroponic platform, the system enabled lettuce to utilize atmospheric nitrogen as an ammonia fertilizer. The net photosynthetic rate, total fresh weight, total chlorophyll content, and total soluble protein content of lettuce grown in the established platform increased by 1.20-, 1.12-, 1.14-, and 1.32-fold, respectively, contributing to the sustainable development of agriculture.
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Tiantian Hu,Yingfei Pu,Yujie Qin
To address the long-term instability of the Navicula -bacteria symbiosis system, this study achieved stable autotrophic nitrogen removal by adding silicates and regulating light/dark cycles. The nitrite accumulation rate reached 92.05 % during the enrichment of Navicula sp. (a diatom genus), which became the dominant algae with the supplementation of silicate and enhanced extracellular polymeric substances (EPS) production. The increased EPS promoted the formation of dense biofilms, facilitating the growth of anaerobic microorganisms. When the ratio of light/dark was adjusted to 8 h:16 h, the system achieved the highest total nitrogen removal rate of 82.69 %. Nitrosomonas (1.26 %) was the dominant bacteria in ammonia-oxidizing bacteria. Denitratisoma (3.75 %) was the dominant bacteria in denitrifying bacteria. Anaerobic ammonia-oxidizing bacteria were naturally enriched without artificial inoculation. The relative abundance of Candidatus Brocadia reached 7.99 %. This study demonstrates an autotrophic pathway for sustainable wastewater treatment.
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2014
·Vol. 22
·期 1
·275-285
·
IF 2.757
Li Wenfeng,Cui Zhaojie,Han Feng
The available energy resources are being depleted worldwide. Industrial symbiosis (IS) provides a promising approach for increasing the efficiency of energy utilization, with numerous studies reporting the superiority of this technology. However, studies quantifying the energy-saving efficiency of IS remain insufficient. This paper proposes an index system for the quantitative evaluation of the energy-saving efficiency of IS. Both energy-saving and financial indexes were selected, the former include the IS energy-saving index, the contribution rate of energy saved through IS, fractional energy savings, and cut rate of energy consumption per total output value; and the latter include the IS investment payback period, IS input–output ratio, net present value (NPV), and internal rate of return (IRR) of IS. The proposed methods were applied to a case study on the XF Industrial Park (XF IP), in the city of Liaocheng in Shandong Province of China. Three energy-saving channels using IS were found in the XF IP: (a) utilizing the energy of high-temperature materials among industrial processes, (b) recovering waste heat and steam between different processes, and (c) saving energy by sharing infrastructures. The results showed that the energy efficiency index of IS was 0.326, accounting for 34.6 % of the comprehensive energy-saving index in 2011, and the fractional energy-savings were 12.42 %. The index of energy consumption per total industrial output value varied from 90.9 tce/MRMB to 51.6 tce/MRMB. Thus, the cut rate of energy consumption per total industrial output value was 43.42 %. The average values of the IS input–output ratio was 406.2 RMB/tce, 57.2 % lower than the price of standard coal. Static investment payback period in the XF IP was 8.5 months, indicating that the XF IP began to earn profit 8.5 months after the construction of all IS modes. The NVP and IRR of each IS mode in the XF IP were greater than zero, with average values equal to 1,789.96 MRMB and 140.96 %, respectively. The computation result for each indicator revealed that IS could lead to the use of energy with high efficiency and lighten the financial burden of enterprises in the XF IP. And the proposed index system may help IPs and EIPs to make strategic decisions when designing IS modes.
研究领域:
能源工程
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工业生态学
·
可持续发展
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环境管理
·
资源效率
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Jingyu Si,Changhu Xue,Qingrong Huang,Zihao Wei
Conventional co-delivery systems often fail to survive in harsh physiological environments due to the lack of underlying mechanistic synergy. Inspired by natural symbiotic paradigms, this study evaluated a mutualistic assembly combining astaxanthin liposomes (AL) with coated probiotics (CP) to investigate their defensive behaviors under hydrogen peroxide–induced oxidative stress. In oxidative stress models, this system exhibited robust phenotypic protection, significantly preserving probiotic viability and mitigating biomolecular and structural damage, as evidenced by reduced membrane lipid peroxidation, protein carbonylation, and lactate dehydrogenase leakage. Interfacial thermodynamic and spectroscopic analyses confirmed that an oxidative stress-induced entropy-dominated mode dictated the dynamic enrichment and stable anchoring of AL on the bacterial surface, which in turn facilitated the localized antioxidant action of astaxanthin to efficiently neutralize destructive reactive oxygen species including hydroxyl radicals. Furthermore, high–resolution mass spectrometry elucidated the bidirectional synergistic defense characteristics of this mutualistic symbiotic system, demonstrating that by establishing a balanced network of oxidative interception and microenvironmental chemical shielding, the components jointly constructed a highly resilient assembly. Taken together, this study provided robust theoretical and strategic support for the development of advanced probiotic delivery platforms with exceptional environmental resilience.
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DOI
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 8
·2105194
·
IF 17.521
Ce Ji,Minglong Qiu,Huitong Ruan,Cuidi Li,Liang Cheng,Juan Wang,Changwei Li,Jin Qi,Wenguo Cui,Lianfu Deng
Three dimension (3D) printed scaffolds have been shown to be superior in promoting tissue repair, but the cell-level specific regulatory network activated by 3D printing scaffolds with different material components to form a symbiosis niche have not been systematically revealed. Here, three typical 3D printed scaffolds, including natural polymer hydrogel (gelatin-methacryloyl, GelMA), synthetic polymer material (polycaprolactone, PCL), and bioceramic ( β -tricalcium phosphate, β -TCP), are fabricated to explore the regulating effect of the symbiotic microenvironment during bone healing. Enrichment analysis show that hydrogel promotes tissue regeneration and reconstruction by improving blood vessel generation by enhancing oxygen transport and red blood cell development. The PCL scaffold regulates cell proliferation and differentiation by promoting cellular senescence, cell cycle and deoxyribonucleic acid (DNA) replication pathways, accelerating the process of endochondral ossification, and the formation of callus. The β -TCP scaffold can specifically enhance the expression of osteoclast differentiation and extracellular space pathway genes to promote the differentiation of osteoclasts and promote the process of bone remodeling. In these processes, specific biomaterial properties can be used to guide cell behavior and regulate molecular network in the symbiotic microenvironment to reduce the barriers of regeneration and repair.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Junhao Xie,Hao Wu,Jinqiu Tao,Xudong Liu,Yunbo Tong,Shuai Qi,Qianping Ran
The incorporation of self-healing technology into antifouling coatings is crucial for the extension of their service life and durability. However, the challenge lies in the insufficient coexistence of strength and toughness hinders their long-term serviceability. Here, this study proposes a cross-scale interfacial engineering strategy for self-healing antifouling coatings inspired by the multiscale microstructure of natural biomaterials The strategy involves the in-situ growth of antifouling zinc-based metal–organic frameworks (Zn-MOF) within a self-healing polymer network constructed by multiple hydrogen bonds. The synergistic cross-scale interfacial interactions between Zn-MOF and the polymer molecules, combined with the intrinsic antifouling properties of Zn-MOF, significantly enhance the strength, toughness to 27.48 MPa, 277.81 MJ/m 3 , and benefit improving antifouling performance. Moreover, the molecular integration of Zn 2+ -pyridine coordination optimizes the microphase structure of the polymer matrix and embeds the advantage of its fast damage response, thereby further enhancing the robustness, and reaching self-healing efficiency to 91.74 % at 40 °C for 24 h. Overall, the proposed strategy represents a viable approach for the fabrication of highly resilient and tough self-healing antifouling coatings.
研究领域:
材料科学
·
表面工程
·
聚合物科学
·
仿生材料
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产品关联
DOI
高影响因子
Bioactive Materials
·
2026
·
IF 23.6
Zeyuan Jin,Yaqi Zhang,Zilong Zhong,Zipeng Shen,Lei Huang,Haijun Hu,Xiping Chen,Wenxing Liu,Lanjuan Li,Changyou Gao
The escalating global burden of ulcerative colitis (UC) underscores the limitations of conventional anti-inflammatory therapies. Although multi-omics insights have propelled gut microbiota modulation to the forefront of therapeutic innovation, current strategies relying on probiotics or fecal transplants remain constrained by empirical designs due to the lack of spatiotemporal precision and real-time monitoring of microbial metabolic vitality in situ . In this study, BacGuard, a metabolically orthogonal microgel platform, was developed to unify the probiotic surveillance and guided dynamic dose regulation with spatially targeted microbiota-associated metabolic modulation. Our core design featured a β-xylosidase-activated chemiluminescent probe (XOS-CL) that was conjugated with xylooligosaccharide-based hyperbranched polymers (HBXOK) and orally delivered via microgels. Thereby, this system enabled real-time monitoring of probiotic abundance and metabolic activity in the colon through enzyme-responsive signaling, while simultaneously promoting short-chain fatty acid (SCFA) production via redirected bacterial metabolic flux. This dual-action system created a self-reinforcing therapeutic loop and optically quantifying the microbial activity in a dynamic manner. By resolving the causal disconnects between enzymatic activity, microbiota proliferation, and host interactions, the BacGuard bridged the diagnostic metrics to functional therapeutic outcomes. Anchoring both sensing and treatment to microbial metabolic flux, our platform reimagined the precision gut ecosystem engineering, establishing an image-guided dynamic dose regulation framework that actively preserved the microbiota-host symbiosis through in-time and function-adaptive modulation.
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DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Yipeng Liu,Rui Feng,Yuyao Zhao,Xiaoya Guo,Jinghan Ding,Sibi Liu,Yijin Wang,Jinmeng Zhu,Xuanhua Li
Solar-driven sorption-based atmospheric water harvesting (SS-AWH) offers promise for addressing global freshwater scarcity. However, the SS-AWH heavily relies on favorable and sustained solar irradiation; yet real-world solar irradiation exhibits significant spatiotemporal fluctuations, limiting its sustainable application, as non/low-light conditions sharply reduce water productivity. This constraint is fundamentally due to the singleness of the water release pathway via photothermal desorption. Here, a novel dual-mode bio-based Janus aerogel (DBJA) is presented, enabling efficient, all-weather, multi-scenario atmospheric water harvesting via selectively solar-driven and compression-activated water release. The Janus structure optimizes mass/heat transfer between hygroscopic and photothermal domains, achieving the most balanced adsorption–desorption kinetics and compression-recovery strength for solar-mechano symbiosis. Under favorable sunlight, DBJA demonstrates a competitive water release efficiency of 1.32 g g −1 day −1 outdoors. Crucially, without solar irradiation, DBJA achieves a total water productivity of 12.80 g g −1 over 5-cycle adsorption-compression with 98% volume recovery and is stable within 50 cycles. Enhanced physical inlay and multiple chemical interactions ensure limited leakage of Li + ions during compression, and the collected water easily conforms to the World Health Organization (WHO) drinking water standards. This work provides a flexible approach for sustainable atmospheric water harvesting beyond solar reliance through multi-mode synergy and gradient architecture.
研究领域:
材料科学
·
环境工程
·
化学工程
·
可再生能源
·
纳米技术
·
可持续技术
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产品关联
DOI
PubMed
高影响因子
WATER ENVIRONMENT RESEARCH
·
2019
·Vol. 91
·期 8
·770-779
·
IF 1.24
Jinhong Fan,Hehe Qin,Yunfei Zhang,Simin Jiang
In this study, Fe/Cu bimetal composite was prepared by high-energy ball milling (BM) method for the removal of refractory organics. The BM Fe/Cu bimetal was characterized by SEM-EDS, XRD, and XPS. Evenly distributed Fe and Cu was observed in the EDS mapping. In contrasting experiments, the removal rate of 4-chlorophenol (4-CP) by BM Fe/Cu materials was about 10-fold faster than that by chemical substitution deposition (CSD) of Fe/Cu material. Complete 4-CP removal and 66.7% of total organic carbon (TOC) mineralization in the BM Fe/Cu–O 2 system were achieved. Dissolved oxygen plays a crucial role for 4-CP degradation through the in situ generation of reactive oxygen species (ROS) such as H 2 O 2 , ·OH, and via oxygen activation reactions. The predominant reactive radicals were identified to be and ·OH through ESR technique and inhibition experiments. The coexistence of oxidation and reduction of 4-CP in the BM Fe/Cu-O 2 system was proposed. Practitioner points 4-CP removal rate by BM Fe/Cu is 10-fold faster than that by CSD Fe/Cu at the same conditions. Complete 4-CP removal and 66.7% of TOC reduction were achieved. All three ROS including ·OH, , and H 2 O 2 coexisted in the BM Fe/Cu-O 2 system. A harmonious coexistence of oxidation and reduction mechanism was proposed.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
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