查找使用阿拉丁产品的科研文献

ZIF-8 as efficient carriers for polysaccharide from Tetrastigma Hemsleyanum Diels et Gilg in acute lung injury induced by lipopolysaccharides

Yinan Qiu,Jia Gao,Wenhui Chu,Shanshan Xia,Chen Huang,Huayue Zhu,Xiaolong Sun,Yongqian Fu

Acute lung injury (ALI) is a critical respiratory syndrome significantly impacting patient health. Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing, SYQ) is a traditional Chinese medicine and its polysaccharides (SYQP) have demonstrated efficacy in counteracting lipopolysaccharide-induced ALI. This study characterized the structure of SYQP and synthesized the SYQP@ZIF-8 composite using biomimetic mineralization, evaluating encapsulation and release efficiency. The biocompatibility of SYQP@ZIF-8 in vitro was assessed by the CCK-8 colorimetric assay and hemolytic activity. Inflammatory cytokine was measured to evaluate the therapeutic effect. The efficacy of SYQP@ZIF-8 in lung injury was assessed using a mice ALI model. Characterization showed SYQP as a homogeneous α-type polysaccharide, comprising galactose, mannose, glucuronide, glucose, galacturonide, and arabinose, with a molecular weight of 516.94 kDa. SYQP@ZIF-8 exhibited high encapsulation rate (> 90 %), rapid pH-responsive release (within 60 min up to ~100 %), low toxicity and favorable hemolytic characteristics. Furthermore, it demonstrated reduced inflammatory cytokine secretion compared to SYQP, along with a superior inhibitory effect. The outcomes of in vivo experiments, including a decrease in the W/D ratio and LDH activity, further confirmed the efficacy of SYQP@ZIF-8 in treating LPS-induced ALI. In conclusion, SYQP@ZIF-8 released SYQP in acidic inflammatory conditions, outperforming SYQP alone in treating ALI.