Min Li,Weiqi Wang,Mengxiaojun Wu,Jingwen Lei,Xiaolu Lu,Hongmei Wang
In this study Paraccocus versutus XT0.6 was employed to address the mechanism of microbial dissolution and oxidation of stibnite. Results showed that with the growth of XT0.6, pH increased to 9.0 in both microbe-mineral contact (MM) and microbe-mineral non-contact groups (M[M]). Dissolved Sb(III) was released from stibnite, which was subsequently quickly oxidized to Sb(V) completely in MM and partially in M[M] groups. On the contrast, the final pH decreased to 6.5 and 4.9, respectviely, in system amended with extracellular secretion (EM) of XT0.6 and abiotic groups. Dissolution of stibnite and oxidation of Sb(III) were also observed in EM group, suggesting a potential contribution of extracellular enzyme in Sb(III) oxidation. The dissolution and oxidation rates were the highest in MM group, followed by those in M[M], EM and abiotic groups. To be noted, Sb(V) concentration decreased in MM group on the fifth day, which might indicate the formation of Sb(V)-bearing secondary mineral. Genome of XT0.6 consisted of two chromosomes and one plasmid, and most genes responsible for antimony oxidation and antimony resistance were located on the chromosomes. Proteomics analysis of the extracellular secretions indicated the up-regulated proteins were mainly related to electron-transfer, suggesting their potential role in Sb(III) oxidation.
Despite the oxidation mechanism of antimonite (Sb(Ⅲ)) by biosynthesized iron nanoparticles (Fe NPs) has been reported, the impact of coexisting components in acid mine drainage (AMD) on the Sb(III) oxidation by Fe NPs is unknown. Herein, how the coexisting components in AMD affect Sb(Ⅲ) oxidation by Fe NPs was investigated. Firstly, Fe NPs achieved complete oxidation of Sb(Ⅲ) (100%), while only 65.0% of Sb(Ⅲ) was oxidized when As(Ⅲ) was added, due to competitive oxidation between As(Ⅲ) and Sb(Ⅲ), which was verified by characterization analysis. Secondly, the decline in solution pH improved Sb(Ⅲ) oxidation from 69.5% (pH 4) to 100% (pH 2), which could be attributed to the rise of Fe 3+ in solution promoting the electron transfer between Sb(Ⅲ) and Fe NPs. Thirdly, the oxidation efficiencies of Sb(Ⅲ) fell by 14.9 and 44.2% following the addition of oxalic and citric acid , respectively, resulting from the fact that these two acids reduced the redox potential of Fe NPs, thereby inhibiting Sb(Ⅲ) oxidation by Fe NPs. Finally, the interference effect of coexisting ions was studied, where PO 4 3− significantly reduced Sb(Ⅲ) oxidation efficiency due to the occupation of the surface-active sites on Fe NPs. Overall, this study has significant implications for the prevention of Sb contamination in AMD.
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