APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 14
·5907-5916
·
IF 3.67
Qiu Yongting,Wang Zhihong,Liu Feng,Liu Junxia,Tan Kaiting,Ji Ruibo
Allelopathy by hydrophytes can be utilized to control algal blooms. This study was conducted to investigate the allelopathic effect (inhibition) of Scenedesmus quadricauda on Microcystis flos-aquae . When M. flos-aquae was co-cultured with S. quadricauda , the secretion of high-MW biopolymer by M. flos-aquae was inhibited by S. quadricauda . We further identified the allelochemicals and found that 4-tert-butylpyrocatechol (TBC) was the main active ingredient that could inhibit the growth of M. flos-aquae . When the dose of TBC was larger than 0.2 mg/L, almost all of the M. flos-aquae died. Additionally, TBC was found to suppress the growth of M. flos-aquae by disturbing the synthesis and secretion of proteins and polysaccharides and harming the chlorophyll to affect the light harvesting of algal cells. Therefore, TBC has the potential for use as a potential and promising algaecide to restrain the biomass of M. flos-aquae .
研究领域:
水生生物学
·
环境微生物学
·
化学生态学
·
藻类生理学
·
生物防治
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 16
·20545-20553
·
IF 3.056
Xu Wenjing,Tan Liju,Zhao Ting,Zhu Xiaolin,Wang Jiangtao
The expanding production and widespread application of acrylamide caused inevitable release to aquatic ecosystems. Contrary to its extensive attention to human and animal health, the hazards of acrylamide to the aquatic primary productivity have been rarely studied. The potential effects of acrylamide on the marine algae ( Nitzschia closterium ) and the limnetic algae ( Scenedesmus quadricauda ) were investigated by monitoring cell abundance, total chlorophyll content, maximum photosystem II (PSII) quantum yield (Fv/Fm), and reactive oxygen species (ROS). The growth of two algae was significantly inhibited by acrylamide. The 96 h EC 50 of acrylamide on N. closterium and S. quadricauda were 5.50 mg L −1 and 45.3 mg L −1 , and no observed effect concentration (NOEC) were 1.07 mg L −1 and 6.97 mg L −1 , respectively. After 96 h exposure to 50 mg L −1 of acrylamide, the total chlorophyll content declined to approximate 18% ( N. closterium) and 48% ( S. quadricauda ), and Fv/Fm was observed to be 0.35 and 0.32 for N. closterium and S. quadricauda , respectively. ROS was significantly increased following higher exposure concentrations, and its levels increased around 2.1-fold and 1.4-fold following exposure to 5 mg L −1 of acrylamide. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed the visible cell plasmolysis, rupture of the plasma membrane, cell vacuolization, and disintegration of chloroplasts of the algae caused acrylamide.
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PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 8
·1776
·
IF 4.2
Yao Qu,Jiahuan Qian,Zhihua Lu,Ruihong Chen,Sheng Zhang,Jingyuan Cui,Chenyu Song,Haiping Zhang,Yafei Cui
Hydraulic shear has been widely accepted as one of the essential factors modulating phytoplankton growth. Previous experimental studies of algal growth have been conducted at the macroscopic level, and direct observation at the cell scale has been lacking. In this study, an algal-cell dynamic continuous observation platform (ACDCOP) is proposed with a parallel-plate flow chamber (PPFC) to capture cellular growth images which are then used as input to a computer vision algorithm featuring a pre-trained backpropagation neural network to quantitatively evaluate the volumes and volumetric growth rates of individual cells. The platform was applied to investigate the growth ofScenedesmus quadricaudacells under different hydraulic shear stress conditions. The results indicated that the threshold shear stress for the development ofScenedesmus quadricaudacells was 270 µL min−1(5.62 × 10−5m2s−3). Cellular growth was inhibited at very low and very high intensities of hydraulic shear. Among all the experimental groups, the longest growth period for a cell, from attachment to PPFC to cell division, was 5.7 days. Cells with larger initial volumes produced larger volumes at division. The proposed platform could provide a novel approach for algal research by enabling direct observation of algal growth at the cell scale, and could potentially be applied to investigate the impacts of various environmental stressors such as nutrient, temperature, and light on cellular growth in different algal species.
研究领域:
藻类学
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流体动力学
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微生物学
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生物技术
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环境科学
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高影响因子
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2017
·Vol. 74
·期 4
·515-526
·
IF 2.467
Xiang Rong,Shi Junqiong,Yu Yi,Zhang Hongbo,Dong Congcong,Yang Yanjun,Wu Zhongxing
To investigate the effect of bisphenol A (BPA) on Cylindrospermopsis raciborskii (Cyanobacteria) and Scenedesmus quadricauda (Chlorophyta), we grew the two species at BPA concentrations of 0, 0.1, 1, 2, 5, 10, and 20 mg/L and examined their growth, lipid peroxidation, antioxidant enzyme activity, and chlorophyll a fluorescence. The 96-h EC 50 values (effective concentration causing 50% growth inhibition) for BPA in C. raciborskii and S. quadricauda were 9.663 ± 0.047, and 13.233 ± 0.069 mg/L, respectively. A significant reduction in chlorophyll a concentration was found in C. raciborskii and S. quadricauda when BPA concentrations were greater than 1 and 2 mg/L, respectively. Furthermore, F
v / F
m , Δ F / F
m ′, and qP decreased significantly at 10 mg/L BPA in C. raciborskii but started to decrease at 10 mg/L in S. quadricauda. The changes in chlorophyll fluorescence parameters ( α , rETR max ) that were obtained from the rapid light response curves of both algae species showed similar responses to F
v / F
m , Δ F / F
m ′, and qP under BPA-induced stress. Values for all of the chlorophyll fluorescence parameters in S. quadricauda were higher than in C. raciborskii ; however, the nonphotochemical quenching measured in C. raciborskii was considerably higher than it was in S. quadricauda . In addition, lipid peroxidation (determined as MDA content) and antioxidant enzyme activities (SOD and CAT) increased in both species as the BPA concentration increased. These results suggest that C. raciborskii is more sensitive to the effects of BPA than S. quadricauda and that photosystem II might be a target for the activity of BPA in vivo.
研究领域:
环境科学
·
植物生理学
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Haolian Xu,Hong Wang,Zixuan Liang,Hongbin Chen,Donghai Yang,Zhenzhen Tang,Xiaohu Dai
This study posed a novel biomimetic flocculation approach, aiming to efficiently harvest high-quality biomass of Scenedesmus quadricauda cultured with anaerobic digestate. Here, that poly(diallyldimethylammonium chloride) (PDADMAC) could serve as mimetic silicified proteins to spontaneously incorporate nanosilica onto microalgal cells, imparting diatom-like characteristics to S. quadricauda . Compared to the exponential growth phase (day 3), the highest harvesting efficiency (93.49%) was obtained at a lower dosage of PDADMAC (5 mg/g) in the stationary phase (day 6), which was attributed to changes in properties and composition of microalgal LB-EPS. On day 6, the hydrophobic functional groups in LB-EPS provided more binding sites during the flocculation process and formed a network structure of microalgal cells-flocculants-nanosilica. The resulting larger and more stable biomimetic silica shell promoted microalgal flocculation and sedimentation. Compared to conventional harvesting methods (centrifugation, polyacrylamide, alkaline flocculation), this method had the minimal negative impact on harvested biomass, with 9.95% of cell membranes damaged.
研究领域:
环境科学
·
生物技术
·
微生物学
·
化学工程
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PubMed
高影响因子
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