PEST MANAGEMENT SCIENCE
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2026
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IF 4.2
Yayu Ren,Youwu Hao,Guangyao Li,Yaru Song,Xiaohan Wang,Qiya Wang,Meizi Wang
BACKGROUND Peanut southern blight, caused by Sclerotium rolfsii Sacc., is a destructive soil-borne disease that severely threatens peanut yield and production safety. In response to the growing demand for environmentally compatible fungicides with low resistance risk, bioactive peptides have emerged as promising alternatives for disease management. RESULTS In this study, two novel peptides, wmz-1 and wmz-2 , were designed using an amino acid combination strategy, and their bioactivities were systematically evaluated. Although both peptides exhibited moderate in vitro inhibition rates of 28.48% and 26.46% against S. rolfsii at 400 mg L −1 , they showed superior in vivo performance in pot experiments. At 200 mg L −1 , wmz-1 demonstrated protective and curative efficacies of 62.79% and 65.68%, respectively, whereas wmz-2 achieved 53.74% and 46.06%, respectively. To investigate the discrepancy between in vitro and in vivo activity, their effects on peanut plants were further examined. The results showed that both wmz-1 and the amino acid combination significantly promoted plant growth and enhanced the activities of defense-related enzymes, thereby providing experimental evidence supporting the involvement of plant growth promotion and induced defense responses. Moreover, both peptides exhibited low cytotoxicity toward human LO2 liver cells. CONCLUSION These findings highlight the potential of wmz-1 and wmz-2 as effective, low-toxicity and environmentally friendly biopesticide candidates for the sustainable management of peanut southern blight, providing a theoretical basis for further development of peptide-based fungicides. © 2026 Society of Chemical Industry.
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DOI
高影响因子
PEST MANAGEMENT SCIENCE
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2026
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IF 4.2
Rui Zhang,Tongfang Jing,Peng Yi,Kaidi Cui,Leiming He,Xuewei Mao,Yuying Song,Lin Zhou
BACKGROUND Sclerotium rolfsii is a destructive soil-borne fungal pathogen distributed worldwide. Although n -butylidenephthalide (BP) exhibits strong inhibitory activity against S. rolfsii , its agricultural application is hampered by high volatility and poor water solubility. RESULTS To address these barriers, a pathogen-triggered delivery system was engineered using tea polyphenol–iron nanocapsules (TP@Fe NCs). The 277 nm core-shell capsules exhibited enhanced thermal stability and specific responsiveness to S. rolfsii , discharging 86.0% of the cargo under 1.0 m m oxalic acid (OA). In vitro assays confirmed superior bioactivity, with TP@Fe NCs exhibiting a significantly lower median lethal concentration (LC 50 ) of 13.34 mg/L compared to 21.53 mg/L for free BP. In glasshouse trials against peanut southern blight, TP@Fe NCs (300 mg/L) achieved a curative efficacy of 74.68%, significantly outperforming free BP (51.30%). Crucially, the formulation demonstrated excellent biosafety. Peanut seedlings treated with 200–300 mg/L TP@Fe NCs showed no phytotoxicity, maintaining emergence rates of 92.69% and normal root/stem development. Furthermore, acute toxicity tests on zebrafish (4.2–5.0 mg/L) revealed that while 5.0 mg/L free BP caused 100% mortality within 48 h, TP@Fe NCs maintained a > 90% survival rate over 96 h. CONCLUSION This study presents a biorational strategy for precise fungal control and develops an expandable and efficient framework for implementing eco-friendly nanopesticide systems toward sustainable agriculture. © 2026 Society of Chemical Industry.
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Tao Tang,Fanfan Wang,Houyun Huang,Nengneng Xie,Jie Guo,Xiaoliang Guo,Yuanyuan Duan,Xiaoyue Wang,Qingfang Wang,Jingmao You
This study explores the antipathogenic properties of volatile organic compounds (VOCs) produced by Bacillus velezensis LT1, isolated from the rhizosphere soil of Coptis chinensis. The impact of these VOCs on the mycelial growth of Sclerotium rolfsii LC1, the causative agent of southern blight in C. chinensis, was evaluated using a double Petri-dish assay. The biocontrol efficacy of these VOCs was further assessed through leaf inoculation and pot experiments. Antifungal VOCs were collected using headspace solid-phase microextraction (SPME), and their components were identified via gas chromatography–mass spectrometry (GC–MS). The results revealed that the VOCs significantly inhibited the mycelial growth and sclerotia germination of S. rolfsii LC1 and disrupted the morphological integrity of fungal mycelia. Under the influence of these VOCs, genes associated with chitin synthesis were upregulated, while those related to cell wall degrading enzymes were downregulated. Notably, 2-dodecanone and 2-undecanone exhibited inhibition rates of 81.67% and 80.08%, respectively. This research provides a novel approach for the prevention and management of southern blight in C. chinensis, highlighting the potential of microbial VOCs in biocontrol strategies.
研究领域:
植物病理学
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微生物学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Tao Tang,Min Xue,Fanfan Wang,Nengneng Xie,Houyun Huang,Yuanyuan Duan,Xiaoyue Wang,Wuxian Zhou,Qingfang Wang,Jingmao You
Pseudomonas fluorescens HT1, isolated from the rhizosphere soil of Coptis chinensis, exhibits potent antagonistic activity against the causal agent of southern blight, Sclerotium rolfsii. This study investigated that the VOCs produced by P. fluorescens HT1 markedly inhibited mycelial growth by 88.31% and sclerotial germination by 79.17% of S. rolfsii LC1. Gas chromatography–mass spectrometry (GC–MS) analysis identified key VOC constituents, namely, chloromethyl methyl sulfide, propionic anhydride, 2-methyl-5-(methylthio)-furan, 2-decanone, 2-undecanone, isobutyl benzoate, and 3-cyclohexyl-1-propanol, demonstrating potent antifungal activity. RNA-seq analysis further revealed that the VOCs markedly altered the transcriptome of S. rolfsii LC1, specifically downregulating genes associated with growth factor and transmembrane transport. In addition, P. fluorescens HT1 successfully colonized C. chinensis tissues and enhanced host resistance against S. rolfsii. This study elucidated the biocontrol mode of action of P. fluorescens HT1 and provides a basis for developing VOC-based management strategies to manage soilborne southern blight in C. chinensis.
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PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Jinzhe Chang,Xuehua Shao,Xin Wang,Jun Liu,Jian Wu,Muhammad Zeeshan,Shiqi Zhu,Pengpeng Liu,Xiaoran Miao,Pengrui Du,Zhixiang Zhang,Peiwen Zhang
The development of novel, safe, and efficient pest and disease control technologies for agricultural crops remains a pivotal area of research. In this study, by combining ZIF-8 and ZIF-90, a water-stable, pH-responsive bilayer MOF nanoparticle (NP) named Z8@Z90 was created, and tebuconazole (TEB) was added to form T@Z8@Z90, used for controlling peanut southern blight. The loading efficiency of TEB within the T@Z8@Z90 reached 26.15%, enabling rapid release in acidic environments triggered by oxalic acid (OA) secreted by Sclerotium rolfsii. In vitro experiments showed that T@Z8@Z90 can regulate the oxalic acid secretion of S. rolfsii and destroy its cell membrane structure. Additional experiments revealed that T@Z8@Z90 reduced sclerotial formation, decreased the total protein content of sclerotia, and influenced their sensitivity to pesticides, thereby mitigating the risk of reinfection by S. rolfsii. Notably, T@Z8@Z90 exhibited efficient translocation within peanut seedlings, being absorbed through the roots and transported to the leaves. At a concentration of 200 mg/L, T@Z8@Z90 exhibited high safety profiles for peanut seedling growth compared to the TEB suspension. Moreover, T@Z8@Z90 is safer for earthworms than TEB SC. Overall, this study offers valuable insights for the management of soil-borne diseases in agriculture and contributes to the advancement of sustainable agricultural practices.
研究领域:
农业科学
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植物病理学
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纳米技术
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环境科学
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高影响因子
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