SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Ting Qi,Lele Wu,Jiachen Yu,Zongcheng Song,Feng Liu,Jun Li,Xiefa Song,Xian Li
Phosphate, as the main nutrient factor of lake eutrophication brought by pollutants discharged from agriculture and industry, is always considered to be a low-toxicity substance to aquatic animals. But the toxicity mechanism is unclear, and published information is limited. In this study, a 96 h acute stress experiment was conducted on juvenile turbot ( Scophthalmus maximus ) with 0, 10, and 60 mg/L phosphate solutions. Metabonomic analysis revealed that low-dose phosphate (10 mg/L) disrupted glycerophospholipid, purine, and glycolipid metabolism, as well as the tricarboxylic acid (TCA) cycle in juveniles, even at 96 h of stress, which may lead to cell structure damage and signal recognition disorder between cells. Upregulated key genes in the main glycerophospholipid metabolic pathways, which matched the results of the metabolomic study, were detected. Furthermore, low-dose phosphate (10 mg/L) induced oxidative stress and immunotoxicity in fish, resulting in the raising of relevant genes expression such as cat and sod in liver and kidney. In addition, all phosphate-treated groups had induced lesions on gill tissue, as evidenced by pathological observations. In this study on toxic effects on and mechanism of phosphate in aquatic animals using metabolomics, gene expression, and histopathology, we confirm that acute low-dose phosphate could disrupt glycerophospholipid metabolism and induce stress in juvenile turbot. This can provide advice on the amount of phosphate accumulation for marine fish farming and on protecting species diversity and marine ecosystem from the point of view of phosphate toxicity to marine animals.
研究领域:
水生毒理学
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环境科学
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海洋生物学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
·
2015
·Vol. 34
·期 4
·789-794
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IF 0.657
Chang Zhiqiang,Liu Fei,Lian Chun’ang,Zhai Qianqian,Li Jian
The pharmacokinetic profiles and sulfamethoxazole (SMX) acetylation process in turbot reared at 18°C were investigated. Either SMX (parent drug) or its acetylized metabolite, N 4 -acetylsulfamethoxazole (AcSMX), was administered intravascularly to turbot at a dosage of 50 mg/kg BW. Serum concentrations of the parent drug and its metabolite were both measured by HPLC, and the changes in concentration over time were analyzed in two- and non-compartment models because SMX treatment produced multiple peaks. The results demonstrated that the elimination half-life of the parent drugs, SMX and AcSMX, were 159.2 and 5.9 h, respectively. The apparent volume of distribution was 0.2 and 0.8 L/kg, and the clearance was 0.038 and 0.222 L/(h·kg), for SMX and AcSMX, respectively. SMX acetylation in turbot was 2.8%, and the deacetylation of AcSMX was 0.2%. These findings may be useful in optimizing SMX dosage regimens in turbot aquaculture.
研究领域:
水产养殖
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药理学
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DOI
高影响因子
Journal of Ocean University of China
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2015
·Vol. 14
·期 4
·695-702
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IF 0.558
Xu Houguo,Huang Lina,Liang Mengqing,Zheng Keke,Wang Xinxing
A 3-month feeding experiment was conducted in an in-door seawater system to investigate the effect of dietary vitamin E (Ve) on the sperm quality of turbot ( Scophthalmus maximus ). D-α-tocopherol acetate was supplemented to the basal (control) diet (65.14 mg kg −1 Ve) to obtain low and high levels of dietary Ve (244.60 mg kg −1 , LVe; 721.60 mg kg −1 , HVe). Compared with the control, sperm concentration was significantly increased in Ve-supplemented groups (LVe and HVe); while relative sperm volume and testis-somatic index were significantly increased in group HVe only. Sperm motility duration was significantly longer in group HVe than in the control, but no significant difference was observed in percent motility among groups. Sperm size, the uniformity of mitochondrial size, and the integrity of mitochondria cristae and plasma membrane were improved by dietary Ve, especially in HVe. The content of Ve in testis and liver as well as polyunsaturated fatty acids in sperm increased with dietary Ve. These results suggested that dietary Ve, especially at the high level (721.60 mg kg −1 ), significantly improved sperm concentration and motility duration and maintained normal sperm morphology of turbot.
研究领域:
水产养殖学
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营养学
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生殖生物学
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DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Jingjing Zhao,Weijie Chen,Yuanxing Zhang,Qin Liu,Dahai Yang,Zhuang Wang
Neutrophils can capture and kill pathogens by releasing neutrophils extracellular traps (NETs), which play critical roles in anti-microbial infection in mammals; however, the mechanisms involved in NETs formation and its role in anti-bacterial infection in teleost fish remains largely unknown. In this study, to explore the function of NETs in turbot , we established an in vitro bacterial infection model in head kidney derived neutrophils, and found that the haemolysin over-expressed Edwardsiella piscicida ( ethA + ) could induce a robust phenotype of NETs, compared with that in wild type or ethA mutant ( ethA + - ΔethA ) strains. Besides, the NETosis was mediated by ethA + -induced pyroptosis , and arms the ability of bacterial killing in neutrophils of turbot. Moreover, we found that neutrophils elastase (NE) might involves in this pyroptotic signaling, rather than inflammatory Sm caspase . Taken together, this study reveals the important role of pyroptosis in NETs formation in turbot neutrophils, suggesting that NETs formation is a critical immune response during bacterial infection in teleost fish.
研究领域:
免疫学
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海洋生物学
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微生物学
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渔业科学
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DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2025
·
IF 4.3
Ying Tao,Xin Li,Lele Wu,Ting Qi,Xinlu Yue,Xian Li
Intensive agriculture's excessive fertilizer use significantly contributes to coastal nutrient pollution. While phosphorus's ecological impacts are recognized, the toxicity of combined phosphorus nutrients on marine life remains unclear. This study examined phosphorus-potassium fertilizer's (P-K fertilizer) effects on juvenile turbot ( Scophthalmus maximus ) via a 96-hour acute exposure experiment. Exposure solutions were prepared at environmentally relevant concentrations, resulting in elevated seawater phosphorus and potassium levels by 117.25 mg/L and 152.94 mg/L, respectively. Compound fertilizer aggravated multi-tissue damage, such as gill epithelial cell proliferation. Untargeted metabolomics identified that P-K fertilizer disrupts riboflavin metabolism, leading to reduced riboflavin levels. This deficiency impaired the production of flavin mononucleotide and flavin adenine dinucleotide. Meanwhile, excess potassium ions triggered Na + /K + ATPase pump and gene of the renal outer medullary K + channel to maintain ionic balance. Following riboflavin metabolism disruption, juvenile turbot exhibited impaired antioxidant capacity, with significantly decreased activities of glutathione peroxidase (by 54.5 %) and superoxide dismutase (by 12.6 %), a significant increase in catalase activity, obvious accumulation of malondialdehyde, and a reduction in total antioxidant capacity. The changes in related gene expression measured by real-time qPCR were consistent with the observed alterations in enzyme activities. Overall, compared to exposure to PO 4 3− -P alone, the combined P-K fertilizer exposure resulted in more severe disruption of riboflavin metabolism and exacerbated oxidative damage in marine fish, indicating that K + potentiated the adverse effects through synergistic interactions. This work provides critical insights for managing nutrient pollution in marine ecosystems and safeguarding coastal biodiversity and ecosystem services.
研究领域:
海洋生态毒理学
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鱼类生理学
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环境污染物生物学效应
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代谢组学
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氧化应激
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营养生态学
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水生毒理学
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产品关联
DOI
PubMed
高影响因子
Fish and Shellfish Immunology Reports
·
2021
·
IF 0
Jing He,Zhuang Wang,Yanbo Zhao,Jin Yang,Yuanxing Zhang,Qin Liu,Dahai Yang
Poly(I:C) is a kind of chemosynthetic double-stranded RNA (dsRNA) analogue which could act as TLR3 agonist and induce IFN production. It is widely applied in anti-virus treatment and immunoregulation, as well as vaccine adjuvant in farmed animals. However, whether poly(I:C) could activate innate immune response to defense against bacterial infection remains unclear. In this study, we established a feeding trial model with different dose of poly(I:C) in turbot larvae, then challenged with Edwardsiella piscicida after 3–7 weeks resting period. The results show that feeding turbot with poly(I:C) exhibited a stronger inflammatory response and antioxidant stress ability, and significantly elevated the survival rate within the decreased bacterial loads. Importantly, the bacterial infection-induced white feces in hindgut of turbot were significantly alleviated after poly(I:C) feeding, and this administration induced protection could last for about 7 weeks. Taken together, these findings indicate that feeding turbot with poly(I:C) could enhance a long-term intestinal mucosal immunity in response to bacterial infection, suggesting that poly(I:C) might be a promising immunostimulant in aquaculture.
研究领域:
水产养殖学
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免疫学
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DOI
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
·
2026
·
IF 2.4
Yingbo Wu,Weiwei Qian,Qin Liu,Xiaohong Liu
An inulin-aluminum composite adjuvant achieved 61.54% RPS against A. salmonicida. • It enhanced lysozyme, total protein, and specific IgM vs. single adjuvants. • It promoted antigen uptake and upregulated Th1-type cytokines and T cell markers. • This adjuvant offers a safe, effective strategy for fish vaccine development. Inulin is a type of plant polysaccharide that is widely distributed in nature. Research has demonstrated that inulin possesses adjuvant activity, stimulating balanced cellular and humoral immune responses. However, the role of inulin as an adjuvant has been relatively underexplored within aquatic vaccine research. In this study, a composite aluminum adjuvant containing inulin was developed, and an inactivated vaccine against Aeromonas salmonicida was formulated based on this adjuvant. Subsequently, the immunoprotective effects of the vaccine in turbot were evaluated to assess the potential application of inulin as an adjuvant in aquaculture vaccines. As a result, the composite of aluminum and inulin significantly promoted immune cell recruitment and antigen uptake at the injection site. Vaccination with the composite adjuvant significantly reduced mortality following A. salmonicida challenge, achieving a relative percent survival (RPS) of 61.54% compared to the control group. Moreover, the vaccine exhibited increased levels of specific IgM along with elevated serum protein levels and lysozyme activity. Furthermore, gene expression analysis revealed significant upregulation of MHC-I , MHC-IIβ , CD4 , CD8 as well as some cytokines including IL-4 , IL-10 , IL-1β , IFN-γ , IL-12 and TNF-α , indicating a robust humoral and cellular immune response. In brief, these findings suggest that inulin acts as a natural immune stimulant and, when combined with aluminum, confers enhanced protective efficacy compared with either adjuvant alone, while balancing both humoral and cellular immune responses. Further studies are needed to clarify the nature of this complementary effect. This novel composite of adjuvants presents a leading candidate strategy for improving fish vaccination practices within aquaculture aimed at bolstering disease prevention efforts. Further studies are warranted to optimize this formulation and investigate the mechanisms underlying immune enhancement provided by the composite adjuvant system for teleost fish.
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产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Di Mu,Yu Jiang,Jing He,Yuanxing Zhang,Dahai Yang,Qin Liu,Zhuang Wang
Propolis is non-hazardous resinous substance mixture containing bioactive ingredients such as polyphenols, flavonoids and organic acid. It has been widely used as food supplement and immune adjuvant due to its benefits in anti-microbial and immunomodulation . Edwardsiella piscicida is a kind of threatening pathogen which could cause high mortality in turbot . However, whether propolis could enhance the innate immune response against E. piscicida infection in turbot remains unknown. In this study, we found dietary propolis addition could improve the expression of anti-oxidative stress related enzymes, e.g., SOD , CAT and GPT , and relieved the histopathological changes of juvenile turbot after E. piscicida infection. Moreover, propolis addition increased the expression of cytokines such as il-1β , il-6 and tnf-α in different organs of juvenile turbot. Importantly, rescued survival and decreased bacterial loads were observed in propolis feeding group. Taken together, these findings suggest that the important roles of propolis in protecting juvenile turbot from E. piscicida infection, indicating propolis might be applied as a promising immunopotentiator candidate in aquaculture.
研究领域:
水产养殖学
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免疫学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 12
·2826
·
IF 3.267
Jun Mei,Feng Liu,Shiyuan Fang,Weiqing Lan,Jing Xie
The effects of modified atmosphere packaging (MAP) in combination with superchilling (−1.3 °C) on the physicochemical properties, flavor retention, and organoleptic evaluation of turbot samples were investigated during 27 days storage. Results showed that high-CO2packaging (70% or 60% CO2) combined with superchilling could reduce the productions of off-flavor compounds, including total volatile basic nitrogen (TVB-N) and ATP-related compounds. Twenty-four volatile organic compounds were determined by gas chromatography–mass spectrometry (GC/MS) during storage, including eight alcohols, 11 aldehydes, and five ketones. The relative content of off-odor volatiles, such as 1-octen-3-ol, 1-penten-3-ol, (E)-2-octenal, octanal, and 2,3-octanedione, was also reduced by high-CO2packaging during superchilling storage. Further, 60% CO2/10% O2/30% N2with superchilling (−1.3 °C) could retard the water migration on the basis of the water holding capacity, low field NMR, and MRI results, and maintain the quality of turbot according to organoleptic evaluation results during storageKeywords:turbot;modified atmosphere packaging;superchilling;shelf life;quality
研究领域:
食品科学
·
水产学
✓ 已验证
DOI
PubMed
高影响因子
Aquaculture Reports
·
2025
·
IF 3.7
Shihui Pan,Nan Bai,Zezheng Qi,Chuwen Chen,Daisong Ma,Zhenlin Liang,Min Gu
Clostridium autoethanogenum protein (CAP) is a promising protein source for aquaculture. Previous studies proved that high levels of dietary CAP retarded the muscle development in turbot through mammalian target of rapamycin (mTOR)/amino acid response (AAR)/adenosine monophosphate-activated protein kinase (AMPK) pathways. However, how CAP regulating these pathways was still unclear and there was no information on the comprehensive analysis for the mechanisms of CAP on fish muscle. In the present study, the integration of metabolomic and proteomic was applied on the muscle of turbot (initial weight of 150 ± 3 g) fed with fishmeal-based diet or diet with 60 % of fishmeal protein replaced by CAP for 70 days. The present work confirmed that CAP decreased the growth, fillet yield and induced the muscle atrophy. Integrated analysis of metabolomics and proteomics showed that CAP negatively influenced the energy production by inhibiting the key enzymes of glycolysis and mitochondrial respiratory chain. Second, CAP inhibited mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase (PI3K) pathways to down-regulate mTOR pathways and protein synthesis. Third, the amino acid contents in turbot muscle were imbalanced by CAP, which would be perceived by general control nonderepressible 1 in AAR signal pathways. Finally, the up-regulated AAR induced amino acids catabolism through purine-based combined deamination. In conclusion, the present work provided the universal knowledge on the working principle of CAP and laid the foundation for CAP improvement in the future.
研究领域:
水产养殖学
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营养学
·
分子生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Wenru Liu,Jun Mei,Jing Xie
In this study, the microbiological, physicochemical, and flavor changes of turbot ( Scophthalmus maximus ) coated with a composite active coating of locust bean gum (LBG) and sodium alginate (SA) supplemented with daphnetin emulsions (0.16, 0.32, 0.64 mg·mL −1 ) were determined during 18 days of refrigerated storage (4 ± 1 °C). Results showed that LBG-SA coatings containing 0.32 mg·mL −1 daphnetin emulsions could significantly lower the total viable count (TVC), psychrophiles , Pseudomonas spp. and H 2 S-producing bacteria counts , and inhibit the productions of off-flavor compounds including the total volatile basic nitrogen (TVB-N), trimethylamine (TMA) and ATP-related compounds. 32 volatile compounds were identified by solid phase microextraction combined with gas chromatography-mass spectrometer method (SPME-GC/MS) during refrigerated storage and the treated turbot samples significantly lowered the relative content of fishy flavor compounds. Further, the LBG-SA coatings containing daphnetin could also delay the myofibril degradation of the turbot samples. These results indicated that the LBG-SA coatings with 0.32 mg·mL −1 daphnetin were a potential alternative way to improve the quality of turbot during refrigerated storage.
研究领域:
食品科学
·
生物化学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Weijie Chen,Jing Hu,Jianchang Huang,Qin Liu,Qiyao Wang,Yuanxing Zhang,Dahai Yang
Bacterial disease is one of the important factors leading to economic losses in the turbot ( Scophthalmus maximus ) cultivation industry. T lymphocytes are major components of cellular immunity , whereas B lymphocytes produce immunoglobulins (Ig) that are key elements of humoral immune responses against infection. However, the genomic organization of genes encoding T-cell receptors (TCR) and immunoglobulin heavy chains (IgHs) in turbot remains largely unknown. In this study, abundant full-length transcripts of TCRs and IgHs were sequenced by Isoform-sequencing (Iso-seq), and we investigated and annotated the V, D, J and C gene loci of TCRα, TCRβ, IgT, IgM and IgD in turbot. Furthermore, through single-cell RNA sequencing (scRNA-seq) of blood leukocytes, we confirmed that these identified TCRs and IgHs were highly expressed in T/B cell clusters, respectively. Meanwhile, we also identified the IgM + IgD + B and IgT + B cells with differential gene expression profiles and potential functions. Taken together, our results provide a comprehensive understanding of TCRs and IgHs loci in turbot, which will contribute to evolutionary and functional characterization of T and B lymphocytes in teleost.
研究领域:
免疫学
·
分子生物学
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基因组学
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水产养殖
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）