SEPARATION AND PURIFICATION TECHNOLOGY
·
2017
·
IF 3.359
Ying Zhao,Xinping Ouyang,Jing Chen,Lisha Zhao,Xueqing Qiu
The conversion of lignin into value-added aromatic monomers has attracted much attention as these monomers can be potentially used to prepare liquid fuel rather than heavy oil by further hydrodeoxygenation. A new process combining Sephadex and silica gel column chromatography was employed to obtain aromatic monomers with higher purity. It was found that pH and polarity of mobile phase were the most important factors affecting the separation effect for Sephadex G-10 and silica gel column chromatography, respectively. When the pH of mobile phase reached 10.5, the ionization of hydroxyl together with carboxyl groups resulted in electrostatic repulsion between the aromatic compounds and Sephadex G-10 gel, which counteracted the adsorption caused by hydrogen bond between aromatics and gel, causing the increase of the separation effect. Under the condition of pH above 10.5, Sephadex gel column chromatography gave 78.1% of purity of aromatic monomers (PAM) and 99.5% of recovery of aromatic monomers (RAM) due to the elimination of non-size exclusion effect. Silica gel column chromatography was further conducted to obtain 99.8% of PAM and 73.9% of RAM. The polar mobile phase was favorable for eluting aromatic monomers, while the non-polar mobile phase was beneficial to remove oligomers and increasing the PAM.
研究领域:
生物化学
·
环境科学
·
材料科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Hongjuan Li,Ying Liu,Yuetao Yi,Qin miao,Sujing Liu,Feng Zhao,Wei Cong,Chunhua Wang,Chuanhai Xia
In China, Poacynum hendersonii is frequently used as a substitute for Apoacynum venetum L (Luobuma), which is a famous traditional Chinese medicine. Quercetin-3- O -sophoroside and isoquercitrin are two major flavonoids in Poacynum hendersonii leaves. In this work, a suitable method was established for the large-scale preparation of quercetin-3- O -sophoroside (QOS) and isoquercitrin (ISO) from Poacynum hendersonii leaves using macroporous resin combined with Sephadex LH-20 column chromatography. The adsorption/desorption capacities and desorption ratios of six macroporous resins were evaluated using static experiments. The HPD-300 resin had the best adsorption performance because it had the largest surface area, and was selected for further study. Compared with pseudo-first-order and intraparticle diffusion kinetics models, the pseudo-second-order model could better fit the adsorption kinetics of both QOS and ISO on the HPD-300 resin. In addition, the adsorption isotherms of the two compounds on the HPD-300 resin were fitted well to the Langmuir model. Under optimal conditions, the purities of QOS and ISO in the product were increased from 2.16% and 1.26% to 21.34% and 10.70% with recovery yields of 82.1% and 77.3%, respectively. Subsequently, Sephadex LH-20 column chromatography was employed for improving the purities of the two compounds. After separation by Sephadex LH-20 column chromatography, the purities of QOS and ISO achieved 93.5% and 95.6%, respectively.
研究领域:
药学
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化学工程
·
天然产物化学
·
中药学
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DOI
PubMed
高影响因子
Current Research in Food Science
·
2023
·
IF 6.3
Yuchen Shangguan,Jing Ni,Lili Jiang,Yang Hu,Chuanbo He,Ying Ma,Guohong Wu,Hejian Xiong
In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed according to the Box-Behnken design of response surface methodology. The experimental conditions for flavonoid extraction were optimized, and naringin was separated from the extracted flavonoids using Sephadex LH-20 column chromatography. Experimental results showed that the influence of factors on the extraction rate of flavonoids from pomelo peels was in the order of ethanol concentration > solid-to-liquid ratio > extraction time, and the optimal extraction parameters were 85% ethanol concentration, 1:20 solid-to-liquid ratio, and 4-h extraction time for extracting flavonoids from pomelo peels. Under these conditions, the yield of flavonoids was 6.07 ± 0.06 mg/g. After three times of extraction, the flavonoid extraction rate reached 96.55%, and the residual naringin in the pomelo peels was 0.017 mg/g, at which point the bitterness in the pomelo peels disappeared. Two components, namely, PF1 and PF2, were separated from the crude flavonoid of pomelo peels through Sephadex LH20 column chromatography. PF2 was identified as naringin by high-performance liquid chromatography tandem mass spectrometry, with a purity of 95.7 ± 0.23%. Both flavonoids and PF2 exhibited good in vitro radicals scavenging activities on DPPH, ABTS, superoxide anion and hydroxyl.
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Hao Xie,Nurmamat Eliyas
To address the low extraction yield and structural degradation issues of conventional methods, this study optimized microwave-assisted extraction (61°C, 64 min, 30:1 mL/g ratio) to obtain Inonotus obliquus polysaccharides (IOP), followed by DEAE-52 and Sephadex G-100 purification to isolate two fractions: neutral IOP-1 (12.67 kDa) and acidic IOP-2 (26.13 kDa). Monosaccharide analysis revealed both as heteropolysaccharides containing mannose, glucose, and galactose with distinct compositional ratios. Structural characterization showed IOP-1 primarily contains 1,4-Glcp, 1,3,6-Glcp, and 1,3-Manp linkages, while IOP-2 features 1,3-Glcp, 1,4-Glcp, 1,4,6-Glcp, and 1,4-Galp linkages. Multiple antioxidant assays demonstrated superior free radical scavenging capacity in both polysaccharides, with IOP-1 exhibiting exceptional activity exceeding typical polysaccharides performance. These findings highlight IOP-1's potential as a novel natural antioxidant for pharmaceutical and food applications. Graphical
研究领域:
食品科学
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药学
·
生物化学
·
天然产物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 410
·期 6
·1817-1824
·
IF 3.431
Jiang Jia,Tian Sizhu,Wang Kun,Wang Yang,Zang Shuang,Yu Aimin,Zhang Ziwei
With the help of iron oxide nanoparticles, electron spin resonance spectroscopy (ESR) was applied to immunoassay. Iron oxide nanoparticles were used as the ESR probe in order to achieve an amplification of the signal resulting from the large amount of Fe 3+ ion enclosed in each nanoparticle. Rabbit IgG was used as antigen to test this method. Polyclonal antibody of rabbit IgG was used as antibody to detect the antigen. Iron oxide nanoparticle with a diameter of either 10 or 30 nm was labeled to the antibody, and Fe 3+ in the nanoparticle was probed for ESR signal. The sepharose beads were used as solid phase to which rabbit IgG was conjugated. The nanoparticle-labeled antibody was first added in the sample containing antigen, and the antigen-conjugated sepharose beads were then added into the sample. The nanoparticle-labeled antibody bound to the antigen on sepharose beads was separated from the sample by centrifugation and measured. We found that the detection ranges of the antigen obtained with nanoparticles of different sizes were different because the amount of antibody on nanoparticles of 10 nm was about one order of magnitude higher than that on nanoparticles of 30 nm. When 10 nm nanoparticle was used as probe, the upper limit of detection was 40.00 μg mL −1 , and the analytical sensitivity was 1.81 μg mL −1 . When 30 nm nanoparticle was used, the upper limit of detection was 3.00 μg mL −1 , and the sensitivity was 0.014 and 0.13 μg mL −1 depending on the ratio of nanoparticle to antibody.
Schematic diagram of procedure and ESR spectra
研究领域:
分析化学
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纳米技术
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生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Qixian Zhang,Qingyong Sun,Xulin Duan,Yuanlong Chi,Bi Shi
Previous research had proved that collagen fiber can be used as separation material for removing caffeine from tea polyphenol extract effectively. But the recovery rate of catechin compounds from collagen fiber column was quite low by using water and ethanol as eluent because of excessively strong hydrogen bond interaction between collagen fiber and polyphenols. To solve the problem, collagen fiber was hydrophobically modified in this work by using silane coupling agents with alkyl chains (butyl, octyl, dodecyl and octadecyl). FT-IR, DSC, SEM and EDS Mapping proved the successful grafting of alkyl chains on collagen fiber, leading to the significantly enhanced hydrophobicity of collagen fiber. Static adsorption showed that the adsorption capacity of EGCG decreased when the grafted alkyl chain length increased mainly relying on the weakened hydrogen bond interaction. Dynamic adsorption and elution behavior also indicated less retention capacity of EGCG on collagen fiber with higher hydrophobicity. In column separation of simulated tea polyphenol extract containing caffeine and catechin compounds, the collagen fiber grafted with octadecyls (CF(C18)) presented excellent separation efficiency for caffeine and catechin compounds, and simultaneously the catechin compounds were highly recovered. The recovery rates of EGC and EGCG were 2–7 times higher than those separated by pristine collagen fiber column and glutaradehyde crosslinked collagen fiber column developed in our previous work. In the reusing round of CF(C18) column, the chromatogram was almost the same as the first round, showing excellent reusability. In fact, the separation performance of CF(C18) to caffeine and catechin compounds was comprehensively superior to Sephadex LH-20, presenting great practical application potential in effectively removing caffeine from tea polyphenol extract and recovering catechin compounds.
研究领域:
化学工程
·
材料科学
·
食品科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2016
·
IF 2.687
Bin Chen,Qing Ye,Kangping Zhou,Yefu Wang
A novel type of aptamer-functionalized immunoadsorbent was prepared and characterized to remove HCV particles, a promising option of extracorporeal immunoadsorption (ECI) therapy against HCV. Herein, we fabricated a HCV-specific immunoadsorbent where single-stranded DNA aptamers reported and studied previously, modified with amino group at the 5′ terminus, was immobilized covalently onto surfaces of carboxylated-derivative sepharose 4FF beads through N -hydroxysuccinimide (NHS) linkage. Then the adsorbents was evaluated and characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Subsequently, we also confirmed that proposed immunoadsorbents exhibited a favorable biocompatibility as well as specificity. In addition, time-dependent effects of the eradication capacity of aptamer functionalized sepharose beads against HCV was investigated. With the optimized time point, the decontamination performance of HCV particles was assessed by real-time quantitative PCR (qPCR) followed by nucleic acid-based hybridization (NAH), which shows sorbents with an aptamer density of 2 nmol ligand/ml resin could remove approximately 80% (i.e. 8.9 × 10 6 HCV particles/ml resin) of the HCV genotype 2a cultivated in vitro and 75% (vary with the intial concentration of HCV from about 7.5 × 10 4 –4.4 × 10 5 HCV particles/ml resin) of the HCV samples from human plasma samples. All these results indicated that the novel aptamer-based adsorbents could effectively remove HCV particles and likely serve as a novel therapy option or at least supplementary for the treatment regimen of HCV.
研究领域:
病毒学
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分子生物学
·
治疗学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Jie Shi,Yazhu Wang,Huanran Wei,Jiajun Hu,Min-Tian Gao
To reduce the production cost of bioethanol and improve the utilization rate of straw resources, more high value-added products have to be found and applied. In this study, tannins were considered as a high-value by-product. The extraction of condensed tannins from rice straw was studied, and the tannins were characterized. The crude tannins consisted mainly of tannins, phenolic acids and monosaccharides. The crude tannins were separated and purified with AB-8 macroporous resin and Sephadex LH-20. The matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) assay confirmed that catechin, epicatechin, epigallocatechin, epicatechin gallate and gallic acid were the major components in the purified tannins. The tannins exhibited strong inhibitory effects on growth of Staphylococcus aureus . Higher concentrations of crude tannins resulted in lower ethanol concentration and intercellular ATP content, and higher residual glucose. Moreover, the tannins could regulate morphology of biofilms. The images from atomic force microscopy and confocal laser scanning microscopy showed that cells hardly adhered to the plate surface, and large aggregations of cells were observed in culture medium containing tannins, and almost all cells were dead. This study provides the feasibility of tannins derived from rice straw as new antimicrobial agents with a different inhibitory mechanism.
研究领域:
生物化学
·
微生物学
·
生物技术
·
药学
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 9
·1681
·
IF 3.06
Mei-Jun Chu,Yong-Mei Du,Xin-Min Liu,Ning Yan,Feng-Zhong Wang,Zhong-Feng Zhang
Due to the importance of proanthocyanidin bioactivity and its relationship with chemical structure, ultrasound-assisted extraction and purification schemes were proposed to evaluate the proanthocyanidin content and analyze the structural composition and potential bioactivities of different proanthocyanidin fractions from Chinese wild rice (Zizanialatifolia). Following an optimized extraction procedure, the crude wild rice proanthocyanidins (WRPs) were purified using n-butanol extraction, chromatography on macroporous resins, and further fractionation on Sephadex LH-20 to yield six specific fractions (WRPs-1–WRPs-6) containing proanthocyanidin levels exceeding 524.19 ± 3.56 mg/g extract. Structurally, (+)-catechin, (−)-epicatechin, and (−)-epigallocatechin were present as both terminal and extension units, and (−)-epicatechin was the major extension unit, in each fraction. This is the first preparation of WRP fractions with a different mean degree of polymerization (mDP), ranging from 2.66 ± 0.04 to 10.30 ± 0.46. A comparison of the bioactivities of these fractions revealed that fractions WRPs-1−WRPs-5 had significant DPPH radical scavenging activities, whereas fraction WRPs-6 with a high mDP showed better α-glucosidase and pancreatic lipase inhibitory effects. These findings should help define possible applications of WRPs to functional foods or nutraceuticals.Keywords:wild rice;Zizania latifolia;proanthocyanidins;extraction;purification;fractionation;degree of polymerization;bioactivities
研究领域:
食品科学
·
植物化学
·
营养保健品
·
生物活性化合物
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yiting Yang,Ping Ren,Ying Sun,Junyi Li,Xinjun Zhou,Haipeng Zhang,Chengguang He,Huining Dai,Lili Guan
Nostoc commune Vaucher, a terrestrial and benthic blue-green alga, widely used in food and medicine worldwide. N. commune Polysaccharides (NCVP) have excellent biological activities, especially immunomodulatory, hypoglycemic and anti-tumor activities. However, the mechanism and structure-activity relationship of NCVP has been less studied. In this study, based on methylation and NMR results, a novel polysaccharide NCVP2 with 135 kDa, containing→4)-α-D-Gal p -(1→, → 4)-β-D-Glc p -(1→, and →4)-α-D-Xyl p -(1→ residues as the backbon, was sequentially purified from N.commune by DEAE-52 and Sephadex G-100 column. NCVP2 (50 μg/mL) exhibited the strong in vitro immunomodulatory activity by promoting the generation of nitric oxide ( NO ) and reactive oxygen species (ROS). A total of 2048 differentially expressed genes (DEGs) were identified by RNA-seq, including 1019 down-regulated genes and 1065 up-regulated genes. These DEGs were mainly enriched in the immune-related biological processes, involving in Mitogen-activated protein kinase (MAPK) and Toll-like receptor (TLR) signaling pathways by GO and KEGG enrichment analysis. Furthermore, Western blot results proved NCVP2 could recognize TLR2 and TLR4/MD2, and regulate TLR7/IRF7, MAPK and PI3K/AKT signaling pathways. In summary, a novel polysaccharide NCVP2 from N.commun e was proposed to exhibit significant immunomodulatory effects with multiple-paths and targets, and has great potential in the development of healthy foods such as immunomodulators.
研究领域:
免疫学
·
生物化学
·
药理学
·
分子生物学
·
植物科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yaojun Ju,Liting Sun,Xiangdi Zhang,Wanning Li,Lihua Hou
To investigate the umami mechanisms and characteristics of soy sauce flavor peptides, four fractions from natural brewed soy sauce were separated using ultrafiltration and Sephadex G-15 gel filtration chromatography. Sensory and ligand-receptor interaction tests showed that the umami strengths of the fractions were related as follows: U1 > U2, G3 > G2, and G3 > U1. Peptide identification revealed that the < 550-Da peptides might be the major contributors to the umami taste of U1 and G3. The higher umami strength of G3 might be attributable to its higher content of umami peptides. G3′s concentration-relative umami intensity curve was plotted using a two-alternative forced choice test. It was also revealed that less sourness, higher saltiness and cool (4 ℃) and hot (50 ℃) serving conditions were conductive to the umami perception of G3. The results could provide a reference for the application of soy-sauce flavor peptides in food.
研究领域:
食品科学
·
化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2024
·Vol. 19
·期 1
·e0297434
·
IF 2.9
Haiyun Jiang,Li Kong,Hongguang Tang,Zhenzhen Wang,Caiping Liu,Jianhui Zhang,Yuxin Chen,Jinyang Shen,Yue Zhou
This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of SPPE was 7.56 mg GAE/g and the polyphenol purity reached 70.5% after macroporous resin and Sephadex LH-20 purification. A total of 50 compounds were identified by UPLC-QTOF-MS/MS. The IC 50 values of SPPE were 334.9 μg/mL, 6.290 μg /mL, 0.834 mg /mL and 0.6538 mg /mL for α-amylase, α-glucosidase, tyrosinase and AchE, respectively. Molecular docking technology further revealed the effects of SPPE on the above enzymes. This study provided information on the potential hypoglycemic, whitening and anti-Alzheimer’s disease biological activities of SPPE, which had guiding significance for the purification and development of other seaweed polyphenols.
研究领域:
海洋生物学
·
植物化学
·
酶学
·
药学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）