JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2017
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IF 2.603
Hongjuan Li,Ying Liu,Yuetao Yi,Qin miao,Sujing Liu,Feng Zhao,Wei Cong,Chunhua Wang,Chuanhai Xia
In China, Poacynum hendersonii is frequently used as a substitute for Apoacynum venetum L (Luobuma), which is a famous traditional Chinese medicine. Quercetin-3- O -sophoroside and isoquercitrin are two major flavonoids in Poacynum hendersonii leaves. In this work, a suitable method was established for the large-scale preparation of quercetin-3- O -sophoroside (QOS) and isoquercitrin (ISO) from Poacynum hendersonii leaves using macroporous resin combined with Sephadex LH-20 column chromatography. The adsorption/desorption capacities and desorption ratios of six macroporous resins were evaluated using static experiments. The HPD-300 resin had the best adsorption performance because it had the largest surface area, and was selected for further study. Compared with pseudo-first-order and intraparticle diffusion kinetics models, the pseudo-second-order model could better fit the adsorption kinetics of both QOS and ISO on the HPD-300 resin. In addition, the adsorption isotherms of the two compounds on the HPD-300 resin were fitted well to the Langmuir model. Under optimal conditions, the purities of QOS and ISO in the product were increased from 2.16% and 1.26% to 21.34% and 10.70% with recovery yields of 82.1% and 77.3%, respectively. Subsequently, Sephadex LH-20 column chromatography was employed for improving the purities of the two compounds. After separation by Sephadex LH-20 column chromatography, the purities of QOS and ISO achieved 93.5% and 95.6%, respectively.
研究领域:
药学
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化学工程
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天然产物化学
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中药学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Qixian Zhang,Qingyong Sun,Xulin Duan,Yuanlong Chi,Bi Shi
Previous research had proved that collagen fiber can be used as separation material for removing caffeine from tea polyphenol extract effectively. But the recovery rate of catechin compounds from collagen fiber column was quite low by using water and ethanol as eluent because of excessively strong hydrogen bond interaction between collagen fiber and polyphenols. To solve the problem, collagen fiber was hydrophobically modified in this work by using silane coupling agents with alkyl chains (butyl, octyl, dodecyl and octadecyl). FT-IR, DSC, SEM and EDS Mapping proved the successful grafting of alkyl chains on collagen fiber, leading to the significantly enhanced hydrophobicity of collagen fiber. Static adsorption showed that the adsorption capacity of EGCG decreased when the grafted alkyl chain length increased mainly relying on the weakened hydrogen bond interaction. Dynamic adsorption and elution behavior also indicated less retention capacity of EGCG on collagen fiber with higher hydrophobicity. In column separation of simulated tea polyphenol extract containing caffeine and catechin compounds, the collagen fiber grafted with octadecyls (CF(C18)) presented excellent separation efficiency for caffeine and catechin compounds, and simultaneously the catechin compounds were highly recovered. The recovery rates of EGC and EGCG were 2–7 times higher than those separated by pristine collagen fiber column and glutaradehyde crosslinked collagen fiber column developed in our previous work. In the reusing round of CF(C18) column, the chromatogram was almost the same as the first round, showing excellent reusability. In fact, the separation performance of CF(C18) to caffeine and catechin compounds was comprehensively superior to Sephadex LH-20, presenting great practical application potential in effectively removing caffeine from tea polyphenol extract and recovering catechin compounds.
研究领域:
化学工程
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材料科学
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食品科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2020
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IF 4.244
Jie Shi,Yazhu Wang,Huanran Wei,Jiajun Hu,Min-Tian Gao
To reduce the production cost of bioethanol and improve the utilization rate of straw resources, more high value-added products have to be found and applied. In this study, tannins were considered as a high-value by-product. The extraction of condensed tannins from rice straw was studied, and the tannins were characterized. The crude tannins consisted mainly of tannins, phenolic acids and monosaccharides. The crude tannins were separated and purified with AB-8 macroporous resin and Sephadex LH-20. The matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) assay confirmed that catechin, epicatechin, epigallocatechin, epicatechin gallate and gallic acid were the major components in the purified tannins. The tannins exhibited strong inhibitory effects on growth of Staphylococcus aureus . Higher concentrations of crude tannins resulted in lower ethanol concentration and intercellular ATP content, and higher residual glucose. Moreover, the tannins could regulate morphology of biofilms. The images from atomic force microscopy and confocal laser scanning microscopy showed that cells hardly adhered to the plate surface, and large aggregations of cells were observed in culture medium containing tannins, and almost all cells were dead. This study provides the feasibility of tannins derived from rice straw as new antimicrobial agents with a different inhibitory mechanism.
研究领域:
生物化学
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微生物学
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生物技术
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药学
✓ 已验证
DOI
高影响因子
MOLECULES
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2019
·Vol. 24
·期 9
·1681
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IF 3.06
Mei-Jun Chu,Yong-Mei Du,Xin-Min Liu,Ning Yan,Feng-Zhong Wang,Zhong-Feng Zhang
Due to the importance of proanthocyanidin bioactivity and its relationship with chemical structure, ultrasound-assisted extraction and purification schemes were proposed to evaluate the proanthocyanidin content and analyze the structural composition and potential bioactivities of different proanthocyanidin fractions from Chinese wild rice (Zizanialatifolia). Following an optimized extraction procedure, the crude wild rice proanthocyanidins (WRPs) were purified using n-butanol extraction, chromatography on macroporous resins, and further fractionation on Sephadex LH-20 to yield six specific fractions (WRPs-1–WRPs-6) containing proanthocyanidin levels exceeding 524.19 ± 3.56 mg/g extract. Structurally, (+)-catechin, (−)-epicatechin, and (−)-epigallocatechin were present as both terminal and extension units, and (−)-epicatechin was the major extension unit, in each fraction. This is the first preparation of WRP fractions with a different mean degree of polymerization (mDP), ranging from 2.66 ± 0.04 to 10.30 ± 0.46. A comparison of the bioactivities of these fractions revealed that fractions WRPs-1−WRPs-5 had significant DPPH radical scavenging activities, whereas fraction WRPs-6 with a high mDP showed better α-glucosidase and pancreatic lipase inhibitory effects. These findings should help define possible applications of WRPs to functional foods or nutraceuticals.Keywords:wild rice;Zizania latifolia;proanthocyanidins;extraction;purification;fractionation;degree of polymerization;bioactivities
研究领域:
食品科学
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植物化学
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营养保健品
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生物活性化合物
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产品关联
DOI
PubMed
高影响因子
PLoS One
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2024
·Vol. 19
·期 1
·e0297434
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IF 2.9
Haiyun Jiang,Li Kong,Hongguang Tang,Zhenzhen Wang,Caiping Liu,Jianhui Zhang,Yuxin Chen,Jinyang Shen,Yue Zhou
This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of SPPE was 7.56 mg GAE/g and the polyphenol purity reached 70.5% after macroporous resin and Sephadex LH-20 purification. A total of 50 compounds were identified by UPLC-QTOF-MS/MS. The IC 50 values of SPPE were 334.9 μg/mL, 6.290 μg /mL, 0.834 mg /mL and 0.6538 mg /mL for α-amylase, α-glucosidase, tyrosinase and AchE, respectively. Molecular docking technology further revealed the effects of SPPE on the above enzymes. This study provided information on the potential hypoglycemic, whitening and anti-Alzheimer’s disease biological activities of SPPE, which had guiding significance for the purification and development of other seaweed polyphenols.
研究领域:
海洋生物学
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植物化学
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酶学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）