CORROSION SCIENCE
·
2024
·
IF 7.4
Xiangping Hao,Yixin Qian,Yun Bai,Ning Wang,Hongchang Qian,Yuntian Lou,Luning Wang,Dawei Zhang
This study reports the effect of extracellular electron transfer (EET) on biomineralization mediated by Shewanella putrefaciens in damaged coatings under different carbon source conditions. S. putrefaciens was able to obtain energy from metal surfaces to support metabolism via EET when the carbon source was insufficient. Under these conditions, bacteria colonized the coating scratches, resulting in accelerated corrosion instead of mineralization. The EIS results revealed that biomineralization was most significant in the 75% carbon source medium after 7 days of incubation. Interestingly, the pH of the medium increased with an abundance of carbon sources, which promoted biomineralization.
研究领域:
微生物学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Xiaoxue Zhang,Mengmeng Zhong,Rui Zhou,Wenxiu Qin,Youbin Si
Microbial reduction of Se(IV) to Se(0) is extremely important for the detoxification of selenium oxyanion, but the reductive mechanism is still controversial. In this study, the reduction of Se(IV) by Shewanella oneidensis MR-1 and Shewanella putrefaciens was systematically investigated. The results showed that excessive Se(IV) severely inhibited the growth of microorganisms and Se(IV) reduction. The extracellular accumulation of Nano-Se(0) particles was shown to be approximately 250–650 nm in diameter. Riboflavin facilitated Se(IV) reduction, while rotenone , as an electron inhibitor, could completely inhibit Se(IV) reduction, validating the extracellular electron transport process for biosynthesized Nano-Se(0). Importantly, the amorphous Nano-Se(0) synthesized by S. oneidensis MR-1 were mixtures of helical chains and Se 8 rings, while those synthesized by S. putrefaciens were mainly composed of helical chains. FTIR results demonstrated that Nano-Se(0) particles were wrapped by some proteins, organic substances and lipids , exhibiting superior dispersibility and biocompatibility. Biogenic Nano-Se(0) particles can also be considered for technological applications in various fields.
研究领域:
微生物学
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环境科学
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产品关联
DOI
高影响因子
Materials
·
2024
·Vol. 17
·期 7
·1501
·
IF 3.1
Xiaohai Wang,Zhuanzhuan Shi,Zhikai Wang,Xiaoshuai Wu
The microbial hybrid system modified by magnetic nanomaterials can enhance the interfacial electron transfer and energy conversion under the stimulation of a magnetic field. However, the bioelectrocatalytic performance of a hybrid system still needs to be improved, and the mechanism of magnetic field-induced bioelectrocatalytic enhancements is still unclear. In this work, γ-Fe2O3magnetic nanoparticles were coated on aShewanella putrefaciensCN32 cell surface and followed by placing in an electromagnetic field. The results showed that the electromagnetic field can greatly boost the extracellular electron transfer, and the oxidation peak current of CN32@γ-Fe2O3increased to 2.24 times under an electromagnetic field. The enhancement mechanism is mainly due to the fact that the surface modified microorganism provides an elevated contact area for the high microbial catalytic activity of the outer cell membrane’s cytochrome, while the magnetic nanoparticles provide a networked interface between the cytoplasm and the outer membrane for boosting the fast multidimensional electron transport path in the magnetic field. This work sheds fresh scientific light on the rational design of magnetic-field-coupled electroactive microorganisms and the fundamentals of an optimal interfacial structure for a fast electron transfer process toward an efficient bioenergy conversion.
研究领域:
微生物学
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生物能源
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2022
·Vol. 46
·期 11
·e17133
·
IF 2.609
Qingyi Wei,Xiaomei Wang,Kaiqiang Wang,Hongbin Pu,Da-Wen Sun
Biofilms formed on food contact surfaces cause significant economic loss in the food sector and pose a serious public health concern. In this study, the formation of Shewanell a putrefaciens ( S. putrefaciens ) biofilms on nylon film at 30°C and 4°C was investigated. Results indicated that more biofilms were formed at 4°C, which peaked after 60 h incubation. The protease activity in biofilms was significantly higher than that in the corresponding plankton culture, and it was closely related to the development stage of biofilms. Besides, S. putrefaciens biofilms formed on nylon film in large yellow croaker juice and fresh cubes at 4°C were also investigated, and SDS-PAGE patterns demonstrated that S. putrefaciens biofilms showed a higher capacity to decompose proteins than planktonic bacteria. This work provided insight into the development of biofilms on nylon film, allowing for further controlling S. putrefaciens biofilms in food packages in the future. Practical applications Flexible plastic packaging with nylon film is widely used for packaging perishable food products including marine fish, smoked fish, processed meats such as sausage and bacon, dairy products, and prepared ready meals due to its various advantages of thermoformability, mechanical strength and toughness, transparency, and oxygen barrier properties. However, the formation of biofilms on the packaging surfaces can cause significant economic loss in the food industry and pose serious public health concerns. This study compared the formation of Shewanella putrefaciens biofilms on nylon film at 30°C and 4°C, and the results can provide further insight into the development of biofilms on nylon film, allowing for better control of S. putrefaciens biofilms in food packages, which is thus very beneficial for the food industry and consumers to ensure food safety.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jingxin Ye,Yunfang Qian,Weiqing Lan,Jing Xie
Shewanella putrefaciens and Listeria monocytogenes are concerned by scientists and consumers due to their ability to produce dense biofilms to contaminate and cause food spoilage. The object of this study was to investigate the antimicrobial and anti-biofilm activities of octyl gallate (OG) against the two food-related bacteria. Results showed that the antimicrobial activities of OG were in a dose-dependent manner and the minimum inhibitory concentration (MIC) of S. putrefaciens and L. monocytogenes were 0.2 mmol/L and 0.1 mmol/L, respectively. OG can rapidly penetrate the bacteria and produce reactive oxygen species (ROS). Then, OG and ROS can lead to membrane damage and cell rupture, causing DNA and protein leakage. Our data also strongly suggested that OG not only can inhibit biofilms formation, but also eradicate the performed biofilms. Furthermore, it was shown that OG, as an additive, has great potential in antimicrobial application to prolong the shelf life of large yellow croakers. These prove that OG can combat bacteria and eradicate biofilm against S. putrefaciens and L. monocytogenes .
研究领域:
食品科学
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微生物学
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食品安全
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产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
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2021
·
IF 7.491
Okon Johnson Esua,Jun-Hu Cheng,Da-Wen Sun
A novel method of thermoultrasound-assisted plasma functionalized buffer (PFB) for decontaminating grass carp was evaluated using the Box–Behnken design (BBD) with processing variables including PFB generating voltage (P V ), ultrasound treatment time (U T ) and temperature (T P ). The predicted models were found to be significant ( p < 0.05) and displayed sufficient fitness with experimental data as indicated by non-significant ( p > 0.05) lack of fit and high coefficient of determination (R 2 ≥ 0.97) values. The optimum decontamination conditions for the responses of S. putrefaciens and S. Typhimurium were P V of 66 V, U T of 14.90 min and T P of 60 ℃, achieving reductions of 4.40 and 3.97 log CFU/g, respectively, with a desirability of 0.998. Among the variables, temperature presented higher significance for inactivating bacteria and the production of volatile basic nitrogen and lipid peroxidation under the optimized conditions were within the limits of freshness for grass carp. Additionally, the effects of PFB and the optimized thermoultrasound-assisted PFB decontamination were mild on the microstructure of grass carp with slight ruptures and loose myofibril structures, indicating the potential of thermoultrasound-assisted PFB for seafood products decontamination with reduced processing time.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2026
·
IF 4.5
Guolin Yang,Ling Wei,Liang Liu,Bo Mu,Tao Chen
Bioremediation of uranium-contaminated environments using native bacteria shows great promise. While Shewanella putrefaciens ( S. putrefaciens ) is a known uranium reducing bacterium, the mechanisms and adaptability of indigenous strains from uranium mine tailings remain unexplored. This study isolated a dominant indigenous strain of S. putrefaciens and employed a combined genomic and spectroscopic approach to elucidate its unique uranium fixation mechanism. Microbial diversity analysis confirmed the dominance of Shewanella in the oligotrophic and radioactive tailings. Whole-genome sequencing revealed a significant enrichment of genes related to energy metabolism and stress resistance, providing a genetic basis for its survival and activity. Crucially, by combining advanced spectroscopic techniques with an MtrA gene knockout experiment, we deciphered the specific role of the Mtr pathway in extracellular electron transfer for uranium reduction. Remarkably, the isolated strain achieved a uranium removal efficiency of up to 93% under experimental conditions, demonstrating its high potential for uranium bioremediation. This work not only provides a robust indigenous candidate for bioremediation but also delivers novel mechanistic insights into the uranium transformation processes of indigenous Shewanella , advancing strategies for the application of tailored microbiomes in radioactive waste management.
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DOI
PubMed
高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2023
·Vol. 59
·期 10
·817-829
·
IF 1.2
Jiaran Qi,Xu Yongjie,Chu Gang,Liu Xiaohong,Si Youbin
Abstract The widespread use of penoxsulam has increased the potential for environmental risks. In this study, a microbial fuel cell (MFC) was investigated and optimized for degradation of penoxsulam and corresponding power generation performance. Of the electricity producing bacteria tested, Shewanella putrefaciens was found to be the most suitable, and reached a maximum power of 260.4 mW/m 2 along with a penoxsulam degradation efficiency of 95%. MFC electrical performance was greatly enhanced by increasing the anode carbon felt surface area from 5 to 20 cm 2 , which raised the MFC electrical power from 90.3 to 240.3 mW/m 2 and increased the penoxsulam degradation efficiency in parallel. The initial penoxsulam concentration in the reaction system influenced MFC power generation, where higher initial penoxsulam concentrations inhibited microbial activity, decreased the MFC power performance, and reduced the penoxsulam degradation efficiency. In addition, the MFC reaction system pH influenced MFC electrical performance, and adjusting the pH to 8 increased the peak voltage and power density to 0.687 V and 264.8 mW/m 2 , respectively. S. putrefaciens secretes soluble compounds to mediate electron transfer and the addition of Riboflavin (RF) increases the charge transfer efficiency. Our findings provide a basis for revealing the mechanism by which organic contaminants are degraded in MFCs, and demonstrate the feasibility of MFCs for efficient penoxsulam degradation and power generation.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Li Liu,Yakun Guo,Weijia Zhang,Fan Bai,Jinlin Wang,Ruichang Gao,Xiaoming Jiang,Yuanhui Zhao,Xinxing Xu
This study proposed to investigate the inhibition mechanism of the Paenibacillus provencensis (PP-2) metabolites on the dominant spoilage bacteria of sea bass and the application in preservation. Shewanella (71.11 %) and Pseudomonas (13.54 %) were the main genera. The Shewanella putrefaciens (L-1) and Pseudomonas fragi (L-4) were isolated and purified based on spoilage factors as dominant spoilage bacteria. PP-2 supernatant metabolites at minimum inhibitory concentration (MIC, 150 mg/mL) inhibited L-1 and L-4 by more than 95 % with significant antioxidant properties. The PP-2 metabolites were detected by LC-MS mainly including organic acids and derivatives, lipids molecules, organic heterocyclic compounds, and organic oxides. According to the transmission electron microscope (TEM) results, Additionally, the metabolites demonstrated antioxidant properties and disrupted bacterial cell membranes, highlighting their potential for controlling seafood spoilage. PP-2 metabolites significantly lowered pH ( p < 0.05), TBARS values (0.33 ± 0.01 μmol/g), and TVB-N levels (24.06 ± 2.25 mg/100 g) compared to Nisin-treated and control groups after 6–8 d. After 8 d, colony counts in the PP-2 group (7.90 ± 0.01 log CFU/g) remained below the spoilage threshold. PP-2 metabolites also reduced protein oxidation, with carbonyl content at 0.33 ± 0.01 μmol/g and sulfhydryl content at 5.26 ± 0.18 μmol/g. The metabolites preserved textural properties, showing slower declines in hardness, elasticity, and chewiness, and lower juice leakage (9.37 ± 1.27 %). Metabolomic analysis revealed that PP-2 metabolites disrupted several key bacterial metabolic pathways, leading to bacterial death. These results suggest that PP-2 metabolites enhance seafood preservation by targeting bacterial metabolic processes.
研究领域:
食品科学
·
微生物学
·
海洋生物学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Hongniu Zhou,Qingyun Wang,Li Ni,Wen Zhang
8 μg/mL resveratrol suppresses spoilage potential without affecting growth. • Resveratrol causes apparent flagellar damage in S. putrefaciens JR-6. • Anti-spoilage effects persist after 30 serial passages with resveratrol. • Resveratrol-passaged JR-6 reduces TVB-N and spoilage volatiles in fish fillets. • Transcriptomics and docking suggest multi-target effects of resveratrol. Shewanella putrefaciens is a specific spoilage organism with strong spoilage potential in aquatic products. At a subinhibitory concentration of 8 μg/mL (1/8 MIC), resveratrol significantly reduced adhesion, biofilm formation, swimming motility, and extracellular protease production ability in S. putrefaciens JR-6 without affecting bacterial growth or membrane integrity. These inhibitory effects persisted after 30 serial passages. Transmission electron microscopy revealed shortened and apparently broken flagella in resveratrol-exposed cells. In fish fillet assays, inoculation with resveratrol-passaged strains delayed TVB-N accumulation and markedly reduced the semi-quantitative levels of three characteristic spoilage-associated volatile compounds. Transcriptomic analysis identified 76 differentially expressed genes associated with amino acid and nitrogen metabolism, oxidative phosphorylation, flagellar assembly, biofilm formation, and two-component systems. RT-qPCR confirmed significant downregulation of genes involved in histidine metabolism ( hisB and hisC ), nitrogen metabolism ( gltD and gltB ), oxidative phosphorylation ( coxA and coxC ), two-component systems ( dctM and dctP ), and quorum sensing ( gene3325 ). Molecular docking predicted interactions between resveratrol and the corresponding proteins through hydrogen bonding and hydrophobic contacts. These findings provide insight into the multi-target modulation of spoilage-associated phenotypes by low-concentration resveratrol and support its further evaluation as a natural preservative for aquatic products.
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DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Li Yang Zhou,Shuai Chen,Hui Li,Shu Guo,Yong Di Liu,Jie Yang
S,S-ethylenediamine-N,N-disuccinic acid (EDDS) enhanced reductive dissolution of α-FeOOH by Shewanella putrefaciens CN32 (CN32), resulting in formation of surface-bound Fe(II) species ( Fe II EDDS) to improve reductive dechlorination of carbon tetrachloride (CT). The pseudo-first-order rate constants for bio-reduction extents of α-FeOOH by CN32 in the presence of 1.36 mM EDDS was 0.023 ± 0.0003 d −1 which was higher than without EDDS. The enhancement mechanism of bio-reduction was attributed to the strong complexation ability of EDDS to formed Fe III EDDS, which could be better utilized by CN32. The dechlorination kinetic of CT by Fe II EDDS (2.016 h −1 ) in the presence of 1.36 mM EDDS was 24 times faster than without EDDS. Chloroform were detected as main products for the degradation of CT. The chemical analyses and morphological observation showed that combination between EDDS and Fe 2+ produced Fe II EDDS complex, which had a reductive potential of −0.375 V and significantly enhanced CT dechlorination. The results showed that EDDS played an important role in enhancing the bio-reduction of α-FeOOH to accelerate reductive dechlorination of CT.
研究领域:
环境微生物学
·
污染修复
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2021
·Vol. 131
·期 2
·791-800
·
IF 3.772
Hou W.,Zhang Y.,Zhang Y.,Yue Q.,Wang L.,Min T.,Wang H.
AimsThe purpose of this study was to investigate the regulatory mechanism of ε‐PL on Shewanella putrefaciens.Methods and ResultsProteomics analysis of inhibitory effect of ε‐PL against S. putrefaciens was performed by label‐free quantitative assay based on high‐resolution mass spectrometry (MS). Quantification of 2206 proteins was obtained with high confidence, and a total of 36 differentially expressed proteins (DEPs), with 10 and 26 proteins showing upregulation and downregulation, respectively, were identified. Upon Go functional enrichment, 11, 5 and 8 specific Go terms in biological processes, molecular functions and cellular components were identified, respectively. Six KEGG pathways, including ‘ribosome’, were significantly enriched. Among the ribosome pathway, there were seven DEPs and all of them were distributed on large and small subunits of ribosome.ConclusionsThe significant downregulation of proteins, large subunits of ribosomal proteins RP‐L18, L30 and L27, small subunits ribosomal proteins S16 and S20, and RNA polymerase β' subunit protein rpoC were the critical action sites of ε‐PL to inhibit S. putrefaciens growth.Significance and Impact of the StudyShewanella putrefaciens is one of the representative fish‐spoilage bacteria regardless of fish type, and poses significant problems for the fish brewery. A better understanding of the antibacterial mechanism of ε‐PL on S. putrefaciens could make important contributions to development of biological control strategies of these economically important pathogens.
研究领域:
微生物学
·
蛋白质组学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）