Insufficient angiogenesis always leads to the prolonged or even nonhealing process for diabetic wounds. Hence, the stimulation of angiogenesis in the wound site is an effective therapeutic strategy for the treatment of diabetic wounds. In this study, we reported a spaced-oriented electrospun scaffold with silicon-doped amorphous calcium phosphate nanocoating on the surface (Si-ACP/PM). The results indicated that the Si-ACP/PM scaffold exhibited an alternately random and aligned microstructure: most of the nanofibers densely deposited in a random manner to form columnar embossments, while others loosely aligned between the embossments to construct a three-dimensional porous microstructure. Furthermore, the Si-ACP nanoparticles with the diameter of about 40 nm were well distributed on the surface of each nanofiber, and the Si ions could sustain release from the scaffold. Results revealed that the Si-ACP/PM scaffold can promote the proliferation and migration of human umbilical vein endothelial cells (HUVECs) in vitro. The in vivo study further demonstrated that the Si-ACP scaffold effectively accelerated the wound healing via the promotion of angiogenesis, collagen deposition, as well as re-epithelialization in the diabetic wound bed. Taken together, the study indicates that the spaced-oriented electrospun scaffold with silicon-doped amorphous calcium phosphate nanocoating on the surface, which could significantly stimulate the angiogenesis during the process of diabetic wound healing, shows great potential for the therapy of diabetic wounds.
Zhifang Wu,Xiaokan Wang,Zhe Wang,Changyu Shao,Xiaoting Jin,Leiqing Zhang,Haihua Pan,Ruikang Tang,Baiping Fu
Lab biomineralization should be carried out in an actual clinical practice. This study evaluated self-etch adhesive as a carrier for amorphous calcium phosphate (ACP) nanoprecursors to continuously deliver biomimetic remineralization of self-assembly type I collagen and demineralized dentin. Si-containing ACP particles (Si-ACP) stabilized with polyaspartic acid (PAsp) were synthesized and characterized by transmission electron microscopy (TEM), scanning electron microscopy–energy-dispersive X-ray spectroscopy, Fourier transform infrared analysis, X-ray powder diffractometry, and X-ray phototelectron spectroscopy. The biomimetic remineralization of single-layer reconstituted type I collagen fibrils and demineralized dentin was analyzed by using two one-bottle self-etch dentin adhesives (Clearfil S3 Bond (S3), Kurraray-Noritake; Adper Easy One (AEO), 3 M ESPE) as a carrier loaded (or not, in the case of the control) with 25 wt % of Si-ACP particles. In vitro cytotoxicity assessed by the Cell Counting Kit-8 indicated that the Si-ACP particles had no adverse effect on cell viability. The capacity for Ca and P ions release from cured Si-ACP-containing adhesives (S3, AEO) was evaluated by inductively coupled plasma-atomic emission spectrometry, revealing the successively increasing release of Ca and P ions for 28 days. The intra- and extrafibrillar remineralization of type I collagen and demineralized dentin was confirmed by TEM and selected-area electron diffraction when the adhesives were used as a carrier loaded with Si-ACP particles. Therefore, we propose self-etch adhesive as a novel carrier for ACP nanoprecursors to continuously deliver biomimetic remineralization.
危险品化学品经营许可证（带存储）