MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2241-2248
·
IF 4.58
Zhang Yufeng,Zhou Zhiping,Zheng Jiahong,Li Hongji,Cui Jiuyun,Liu Siwei,Yan Yongsheng,Li Chunxiang
The authors describe a fluorescence based assay for determination of the macrolid antibiotic erythromycin (ERY). It is based in the use of fluorescent gold nanoclusters (AuNCs) coated first with silica (to obtain AuNC@SiO 2 ) and then with a molecularly imprinted polymer (MIP). The MIP was synthesized from 3-aminopropyltriethoxysilane as the functional monomer, ERY as the template, and tetraethoxysilane as the cross-linker via a sol-gel process that leads to surface imprinting. By using this method, the strong fluorescence of AuNCs was maintained in the resultant MIPs. After optimization of the experimental conditions, the signal (measured at excitation/emission wavelengths of 396/585 nm) decreases linearly with increasing concentration of ERY in the 0.1 to 11.9 μM ERY concentration range. The limit of detection is 12 nM, and the imprinting factor is 4.0. The method was successfuly applied to the determination of ERY in spiked human urine and saliva.
Schematic of the preparation of a core-shell sensor material with the silica coated by fluorescent gold nanoclusters as core and a molecularly imprinted polymer. It can be used to selectively detect residual erythromycin in biological samples.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
药物分析
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 4.321
Jie-Ping Fan,Xiao-Kang Xu,Rui Xu,Xue-Hong Zhang,Jian-Hang Zhu
In this work, the superparamagnetic Fe 3 O 4 particles prepared by the coprecipitation method were coated successively by SiO 2 , vinyl end groups and molecularly imprinted polymer (MIP) shells to afford a pomegranate-like core/shell superparamagnetic particle (Fe 3 O 4 @SiO 2 @MIP), and the MIP shell preparation conditions were optimized by multi-objective response surface methodology. In order to simultaneously enrich and separate the four taxoids (10-DAB, baccatin III, cephalomannine and paclitaxel), cephalomannine was selected as the template because of its similar chemical structure to other three taxoids and relative inexpensiveness. Especially, to avoid template leaking which may influence the accuracy of identification and quantitation of the analytes, we selected paclitaxel as the target molecular during the investigation of the adsorption property of Fe 3 O 4 @SiO 2 @MIP particles. This strategy was similar to the dummy molecular imprinting technique in which the dummy molecularly imprinted polymer was prepared by using a dummy template, structural analog of the target analyte. The as-prepared Fe 3 O 4 @SiO 2 @MIP particles were characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FTIR). The adsorption kinetics, isotherms and reusability were also investigated. Finally, the Fe 3 O 4 @SiO 2 @MIP particles were successfully applied to simultaneously enrich and separate these four taxoids in the extract of Taxus × media ( T. × media ).
研究领域:
分析化学
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 18
·4221-4229
·
IF 3.286
Long Zerong,Shen Shilin,Lu Yi,Lan Wei,Chen Jia,Qiu Hongdeng
Herein, rare-earth europium doped in Gd 2 O 3 @SiO 2 -based molecularly imprinted polymer (MIP) composite nanospheres with a multilayer core-shell structure was successfully prepared via a facile and versatile layer-by-layer assembly strategy of combination with sol-gel, hydrothermal, and surface imprinting procedure. The rare-earth Gd 2 O 3 :Eu 3+ was embedded into the inner portion of the imprinted polymer which was well-suited for fluorescent monitoring carbaryl selectively. Results showed that the recognition process of the nanosensor for carbaryl was fast and reached dynamic equilibrium at ca. 20 min. The fluorescence intensity ( F 0 / F ) is linearly related to the concentration of carbaryl [ Q ] within the range of 16–80 μg mL −1 , and the linear equation is F 0 / F = 0.8909 − 9.775 × 10 −4 [ Q ] ( R = 0.9963) with 10 μg mL −1 as the detection limit. Competition experiments showed that other analogues (methomyl, aldicarb, and isoprocarb) have nearly no interference in the detection of carbaryl. Moreover, this MIP nanosensor was successfully applied to detect carbaryl in green tea samples without pretreatment. The study afforded an efficient and desirable fluorescence sensor for carbaryl detection in a complicated matrix, which hopefully will be used for biomedical/chemical sensing recognition.
Graphical abstract
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Shan Wang,Mingfei Pan,Kaixin Liu,Xiaoqian Xie,Jingying Yang,Liping Hong,Shuo Wang
A molecularly imprinted sensor for highly sensitive and selective determination of dibutyl phthalate (DBP) was fabricated by combining multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) with surface molecularly imprinted polymer (SMIPs). The MWCNTs and AuNPs were designed to modify the electrode surface to accelerate the electron transfer rate and enhance the chemical stability. SMIPs were synthesized using SiO 2 microspheres as carriers. By loading SMIPs capable of identifying DBP on the surface of modified electrodes of MWCNTs and AuNPs, an electrochemical sensor for detecting DBP was successfully constructed. After optimizing the experimental conditions, the modified electrode SiO 2 -COOH@MIP/AuNPs/MWCNTs/GCE can recognize DBP in the range of 10 −7 g L −1 to 10 −2 g L −1 , and the detection limit achieved to 5.09 × 10 −9 g L −1 (S/N = 3). The results demonstrate that the proposed MIP electrochemical sensor may be a promising candidate electrochemical strategy for detecting DBP in complex samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Hui Jiang,Donglei Jiang,Jingdong Shao,Xiulan Sun
We have developed a novel and economical electrochemical sensor to measure Gram-negative bacterial quorum signaling molecules (AHLs) using magnetic nanoparticles and molecularly imprinted polymer (MIP) technology. Magnetic molecularly imprinted polymers (MMIPs) capable of selectively absorbing AHLs were successfully synthesized by surface polymerization. The particles were deposited onto a magnetic carbon paste electrode (MGCE) surface, and characterized by electrochemical measurements. Differential Pulse Voltammetry (DPV) was utilized to record the oxidative current signal that is characteristic of AHL. The detection limit of this assay was determined to be 8×10 −10 mol L −1 with a linear detection range of 2.5×10 −9 mol L −1 to 1.0×10 −7 mol L −1 . This Fe 3 O 4 @SiO 2 -MIP-based electrochemical sensor is a valuable new tool that allows quantitative measurement of Gram-negative bacterial quorum signaling molecules. It has potential applications in the fields of clinical diagnosis or food analysis with real-time detection capability, high specificity, excellent reproducibility, and good stability.
研究领域:
分析化学
·
生物传感器
·
微生物学
·
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）