Biomaterials Advances
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2025
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IF 6
Lei Zhan,Boqing Zhang,Hongyun Wo,Lina Wu,Huan Li,Min Liu,Zhuangzhuang Li,Tao Chen,Xingyu Gui,Kaixin Wang,Xiaobing Pu,Changchun Zhou
The aging population and the increasing prevalence of sports-related injuries have made osteochondral defects a major global health issue. The repair of osteochondral defect is still a challenge due to poor regeneration of both cartilage and bone tissues. To address this issue, this study developed a biomimetic dual-layer scaffold based on SilMA hydrogel using DLP technology. For the upper area of scaffold, PEGDA was used to enhance the mechanical strength of SilMA hydrogel, making it more suitable for the environment of articular cartilage, while cell microspheres were incorporated as 3D carriers for chondrocyte encapsulation and delivery, providing seed cells for regeneration. For the lower area of scaffold, the lower part was loaded with hydroxyapatite to promote bone tissue regeneration. This study systematically investigated the design of 3D printable inks for osteochondral defect repair, exploring the effects of PEGDA and HAp on the printability and physicochemical properties of SilMA bioink through orthogonal experiments. In vitro and in vivo results demonstrated that the biomimetic dual-layer scaffold exhibited good biocompatibility, promoting cell adhesion and proliferation. Thus, these dual-layer scaffolds loaded with chondrocyte microspheres effectively promote the synchronous repair of both articular cartilage and subchondral bone.
研究领域:
生物医学工程
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
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2024
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IF 10
Jun Zhou,Lisha Zhang,Yaqi Wei,Qiang Wu,Kaibo Mao,Xiaoli Wang,Jinfeng Cai,Xia Li,Yongxiang Jiang
Bacterial biofilm formation protects bacteria from antibiotics and the immune system, excessive inflammation further complicates treatment. Here, iron-based metal–organic framework (MIL-101)-loaded riboflavin nanoparticles are designed for the therapeutic challenge of biofilm infection and hyperinflammation in bacterial keratitis. Specifically, MIL-101 produces a thermal effect under exogenous near-infrared light irradiation, which synergizes with ferroptosis-like bacterial death induced by iron ions to exert an effective biofilm infection eradication effect. On the other hand, the disintegration of MIL-101 sustains the release of riboflavin, which inhibits the pro-inflammatory response of macrophage over-activation by modulating their phenotypic switch. In addition, to solve the problems of short residence time, poor permeability, and low bioavailability of corneal medication, the MR@MN microneedle patch is further prepared by loading nanoparticles into SilMA hydrogel, which ultimately achieves painless, transepithelial, and highly efficient drug delivery. In vivo and ex vivo experiments demonstrate the effectiveness of this approach in eliminating bacterial infection and promoting corneal healing. Therefore, the MRMN patch, acting as an ocular drug delivery system with the ability of rapid corneal healing, promises a cost-effective solution for the treatment of bacterial keratitis, which may also lead to a new approach for treating bacterial keratitis in clinics.
研究领域:
眼科学
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药学
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生物医学工程
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免疫学
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材料科学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2024
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IF 4.4
Bo Zhang,Jiaxuan Yan,Yi Jin,Yushun Yang,Xiubo Zhao
In this study, a novel approach was employed to develop a therapeutic system for colorectal cancer treatment. Specifically, a GelMA/SilMA hydrogel loaded with curcumin-shellac nanoparticles (Cur@Lac NPs) was created. A microfluidic swirl mixer was utilized to formulate stable Cur@Lac NPs, ensuring their consistent and effective encapsulation. The pH-specific release of curcumin from the NPs demonstrated their potential for colon cancer treatment. By carefully regulating the ratio of GelMA (gelatin methacrylate) and SilMA (silk fibroin methacrylate), a GelMA/SilMA dual network hydrogel was generated, offering controlled release and degradation capabilities. The incorporation of SilMA notably enhanced the mechanical properties of the dual network matrix, improving compression resistance and mitigating deformation. This mechanical improvement is crucial for maintaining the structural integrity of the hydrogel during in vivo applications. In comparison to the direct incubation of curcumin, the strategy of encapsulating curcumin into NPs and embedding them within the GelMA/SilMA hydrogel resulted in more controlled release mechanisms. This controlled release was achieved through the disintegration of the NPs and the swelling and degradation of the hydrogel matrix. The encapsulating strategy also demonstrated enhanced cellular uptake of curcumin, leveraging the advantages of both NPs and in-situ hydrogel injection. This combination ensures a more efficient and sustained delivery of the therapeutic agent directly to the tumor site. Overall, this approach holds significant promise as a smart drug delivery system, potentially improving the efficacy of colorectal cancer treatments by providing targeted, controlled, and sustained drug release with enhanced mechanical stability and biocompatibility.
研究领域:
药学
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生物医学工程
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癌症治疗
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药物递送系统
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纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）