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CARBOHYDRATE POLYMERS · 2019 · IF 6.044

Structural characterization and antioxidant activity of a new polysaccharide from Bletilla striata fibrous roots

Ziyan Chen,Yan Zhao,Mengke Zhang,Xiufang Yang,Pengxiang Yue,Dikang Tang,Xinlin Wei

A new polysaccharide (pFSP) was first isolated from the originally discarded fibrous roots part of Chinese traditional herb, Bletilla striata. pFSP was composed of D-glucose, D-galactose and D-mannose in a molar ratio of 1: 2.03: 3.45 with molecular weight of 9.1 × 10 4 Da. It could effectively scavenge DPPH and superoxide radicals with inhibition rate of 64.47% and 72.27% at 5.0 mg/ml, higher than that of polysaccharide from Bletilla striata tuber. Structural investigations of the periodate oxidation studies and Smith-degradation as well as the FT-IR spectroscopy were performed, and combined with 1D and 2D NMR spectroscopy, the repeating unit of pFSP contained (1→4)-linked-α-D-Glcp, (1→4)-linked-β-D-Manp and (1→3,6)-linked-β-D-Manp units, together with the branches of (1→6)-linked-β-D-Galp and terminated with (1→)-linked-β-D-Manp residue.

研究领域: 药理学 · 生物化学 · 分子生物学 · 分析化学
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