PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Pan-Pan Guo,Xi-Bin Yang,Hong Yang,Cao Zhou,Gui-Yun Long,Dao-Chao Jin
β -N-Acetylglucosaminidases and/or β - N -acetylhexosaminidases (NAGs / Hexes) are crucial exonucleases, playing a crucial role in the insect molting process. SfHex3 and SfHex4 contain conserved catalytic domains of GH20 and GH20b, clustered into NAG2 and NAG1 group, respectively. SfHex3 and SfHex4 were mainly highly expressed in the 4th–5th instar nymphs, as well as in the integument and ovary. The expression level of SfHex3 gradually decreased in male and female adults, and SfHex4 on the first day of female was significantly higher than that on the first day of male. In addition, RNA interference (RNAi) results demonstrated that the downregulation of SfHex3 and SfHex4 expression in 5th-instar nymphs resulted in failed molting, and a high mortality. Furthermore, after RNAi with SfHex3 and SfHex4 , the transcript levels on key genes of the chitin metabolism pathway ( SfCHS1 , SfCHS1a , SfCHS1b , SfTRE1 , SfTRE2 , SfCht5 , and SfCht7 ) were significantly decreased compared to the control group. Meanwhile the expression levels of SfHex3 and SfHex4 were up-regulated after 6 h and 12 h of 20E treatment. And the transcription levels of SfHex3 and SfHex4 were significantly inhibited at nitenpyram LC 20 , LC 50 , and LC 90 after 96 h of treatment, in 3rd nymphs of Sogatella furcifera . In conclusion, SfHex3 and SfHex4 play important roles in the nymphal development of S. furcifera , contributing to the molting process from nymph to adult. This study not only enhances our understanding of the nitenpyram in pest control, but also provides a foundation for the development of new control strategies using RNAi to targeting SfHex3 and SfHex4 .
研究领域:
昆虫学
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分子生物学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yanxin Ma,Changwei Gong,Qiulin Wang,Jian Pu,Jizhi Yang,Anchun Peng,Hongxing Chen,Xuegui Wang
Sogatella furcifera is a migratory pest that harms rice cultivation. Triflumezopyrim (TFM), a novel insecticide, effectively controls S. furcifera. To guide its scientific application, we studied the TFM resistance mechanisms in resistant strains. Through cross-resistance determination, we found that the strain had cross-resistance to clothianidin. Synergistic and enzyme activities also demonstrated that the TFM resistance was related to the enhancement of glutathione S-transferases and UDP-glycosyltransferases. Meanwhile, SfGSTd2, SfUGT2, and SfUGT35A1 were invariably upregulated in the TFM-resistant strain. RNAi revealed that SfGSTd2 (mortality increasing 33.3–53.3%) and SfUGT35A1 (increasing 33.3–46.7%) significantly increased sensitivity on TFM, while SfUGT35A1 (increasing 40.0–46.7%) also clearly promoted sensitivity toward clothianidin in S. furcifera. The GAL4/UAS system further verified that the overexpression of SfUGT35A1 is involved in the formation of TFM (mortality descending 2.8–52.8%) and clothianidin resistance (mortality descending 11.1–41.7%). Therefore, all results showed that the overexpression of SfGSTd2- and SfUGT35A1-mediated resistance to TFM in S. furcifera.
研究领域:
昆虫学
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分子生物学
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害虫管理
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 21
·15722
·
IF 5.6
Wanwan Wang,Haiyun Rui,Lei Yu,Nuo Jin,Wan Liu,Chen Guo,Yumeng Cheng,Yonggen Lou
Chemical elicitors can increase plant defense against herbivorous insects and pathogens. The use of synthetic chemical elicitors is likely to be an alternative to traditional pesticides for crop pest control. However, only a few synthetic chemicals are reported to protect plants by regulating signaling pathways, increasing the levels of defense metabolites and interfering with insect feeding. Here, we found that the exogenous application of a phenoxycarboxylic compound, 4-chlorophenoxyacetic acid (4-CPA), can induce chemical defenses to protect rice plants from white-backed planthoppers (WBPH,Sogatella furcifera). Four-CPA was rapidly taken up by plant roots and degraded to 4-chlorophenol (4-CP). Four-CPA treatment modulated the activity of peroxidase (POD) and directly induced the deposition of lignin-like polymers using hydrogen peroxide (H2O2) as the electron acceptor. The polymers, which are thought to prevent the planthopper’s stylet from reaching the phloem, were broken down by WBPH nymphs. Meanwhile, 4-CPA increased the levels of flavonoids and phenolamines (PAs). The increased flavonoids and PAs, together with the degradation product of the polymers, avoided nymphal feeding and prolonged the nymphal period for 1 day. These results indicate that 4-CPA has the potential to be used as a chemical elicitor to protect rice from planthoppers. Moreover, these findings also open a pathway for molecule structure design of phenoxycarboxylic compounds as chemical elicitors.Keywords:chemical elicitor;4-chlorophenoxyacetic acid;lignin-like polymers;white-backed planthoppers;rice;chemical defense
研究领域:
植物科学
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昆虫学
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农业化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ao Liang,Jie Hu,Zuhan Zhao,Xiangsong Li,Jian Pu,Jizhi Yang,Liping Yu,Xuegui Wang
Sogatella furcifera ( S. furcifera ) is a serious pest of rice in China. Excessive use of conventional insecticides has led to environmental pollution and other associated issues. The rapid advancement of nanotechnology offers new perspectives for developing innovative pest management strategies. Therefore, we designed a nanopesticide system in which dinotefuran (DNF) was encapsulated within chitosan (CS) and further integrated with ZnO quantum dots (ZnO QDs). The resulting formulation (DNF@CS-P-ZnO) displayed an average particle size of 33.3 nm and a loading efficiency of 17.74 %. Under acidic conditions (pH 5), about 74.93 % of DNF was released within 96 h, showing a controlled release profile. Bioassays confirmed high insecticidal activity, with mortality reaching 93.67 % after 4 d. Mechanistic analyses indicated that the nanoformulation inhibited cytochrome P450 activity and downregulated resistance-related genes ( CYP417A2 , CYP439A3 , CYP4CE3 ), thereby enhancing susceptibility to insecticides. Additionally, the formulation exhibited effective translocation within both pests and rice plants, improved photostability, enhanced wettability, and minimal impact on non-target organisms. Overall, DNF@CS-P-ZnO represents a promising nanopesticide platform that improves pesticide efficacy while reducing environmental risks, offering potential for sustainable rice pest control.
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2016
·
IF 2.388
Xi-Chao Mu,Wei Zhang,Li-Xiang Wang,Shuai Zhang,Kai Zhang,Cong-Fen Gao,Shun-Fan Wu
Three rice planthoppers , brown planthopper , Nilaparvata lugens , white-backed planthopper, Sogatella furcifera and small brown planthopper, Laodelphax striatellus , are important pests of cultivated rice in tropical and temperate Asia. They have caused severe economic loss and developed resistance to insecticides from most chemical classes. Dinotefuran is the third neonicotinoid which possesses a broad spectrum and systemic insecticidal activity. We determined the susceptibility of dinotefuran to field populations from major rice production areas in China from 2013 to 2015. All the populations of S. furcifera and L. striatellus were kept susceptible to dinotefuran (0.7 to 1.4-fold of S. furcifera and 1.1-to 3.4-fold of L. striatellus ) However, most strains of N. lugens (except FQ15) collected in 2015 had developed moderate resistance to dinotefuran, with resistance ratios (RR) ranging from 23.1 to 100.0 folds. Cross-resistance studies showed that chlorpyrifos-resistant and buprofezin-resistant Sogatella furcifera , chlorpyrifos-resistant and fipronil-resistant L. striatellus , imidacloprid-resistant and buprofezin-resistant Nilaparvata lugens exhibited negligible or no cross-resistance to dinotefuran. Synergism tests showed that piperonyl butoxide (PBO) produced a high synergism of dinotefuran effects in the DY15 and JS15 populations (2.14 and 2.52-fold, respectively). The obvious increase in resistance to dinotefuran in N. lugens indicates that insecticide resistance management strategies are urgently needed to prevent or delay further increase of insecticide resistance in N. lugens .
研究领域:
昆虫学
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农药科学
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农业害虫管理
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昆虫抗药性
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DOI
高影响因子
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