Shihan Qin,Xue Jiang,Shani Li,Qing Li,Jin Huang,Qingjiao He,Wei Cai
The dried root of Sophora davidii Kom. ex Pavol (Leguminosae, family) is a folk medicine and has long been used for the treatment of various diseases, including cough, hematochezia, sore throat, and dysentery. In this study, UHPLC-Q-Exactive Orbitrap mass spectrometry investigations were applied to obtain the profiling of the flavonoids in S. davidii . Finally, 108 compounds were identified, and 85 of them were firstly reported. Then, the in vitro antioxidant activity and tyrosinase activity of S. davidii were investigated using the DPPH radical scavenging ability, hydroxyl radical scavenging, and copper-reducing ability method and by measuring the rate of tyrosinase-catalyzed L-dopa oxidation. To our knowledge, this is the first study of S. davidii’s antioxidant activity, anti-tyrosinase activity, and systematic characterization of flavonoids. The current study will be of support for the usage of S. davidii in the future development of the pharmaceutical industry.
Ethnopharmacological relevance In TCM opinion, most of pneumonia is related to "lung heat". Sophora davidii (Franch.) Skeels flower was first documented in "Guizhou Herbal Medicine", and was recorded as having functions of clearing heat, detoxifying, and cooling blood. It can be used to treat lung heat cough. Aim of the study To investigate main mechanisms of Sophora davidii flower extract (SDFE) in Treating LPS-induced acute Pneumonia. Materials and methods Acute pneumonia models on BEAS-2B cells and rats were established using LPS. The rat model was used to verified the protective effects of SDFE through HE staining, lung tissue W/D ratio assay, white blood cell count analysis, and ammonia-induced coughing test. Network pharmacology was applied to predict the active compounds, core targets and main pathways of SDFE in treating acute pneumonia. Western Blot and ELISA kits were employed to validate representative proteins in selected pathway in vivo and in vitro . Results HE staining, lung tissue W/D ratio assay, white blood cell count analysis, and ammonia-induced coughing test showed SDFE could improve pathological features (leukocyte infiltration, pulmonary edema, lung injury and cough). Network pharmacology indicated MAPK/NF-κB pathway was the most relevant pathway. SDFE could significantly inhibit the expression of Fos and Jun, and the phosphorylation levels of p38, ERK, JNK, NF-κB and IκB. It also down-regulated the expression of pro-inflammatory factors (TNF-α, IL-6 and IL-1β). Conclusions SDFE can exert protective effects against acute pneumonia through the MAPK/NF-κB signaling pathway.
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