Yuanyuan Sun,Lingling Zhao,Chunxia Wu,Yanru Chen,Guang-Li Wang
Photoelectrochemical (PEC) bioanalysis has recently become a perspective pathway for biomolecular sensing, presenting new insights for disease diagnosis and treatment. Herein, we present a new cathodic PEC platform for detecting Src kinase in a label-free, non-immobilized, and high-throughput mode. Specifically, treatment of the tyrosine-containing peptide by tyrosine kinase produced an ortho-diphenol structure of tyrosine that can in situ introduce oxygen vacancies (Ov) on the surface of NiTiO 3 material, which led to the augmentation of the photoelectric signal. While the presence of the target Src kinase phosphorylated tyrosine in substrate peptide and blocked the surface Ov formation onto NiTiO 3 . Such a phenomenon was correlated with the Src kinase detection in terms of good sensitivity and selectivity, achieving the linear detection range of 0.001–2.0 μg/mL and the detection limit of 3.0×10 −4 μg/mL (S/N=3). For Src kinase detection, this is a superior strategy to some classical methods in terms of easy operation, rapid response, and cost effectiveness. This study features the first exploration of cathodic photoelectrochemistry for Src kinase detection and is expected to expand its application in probing more disease related targets with unknown possibilities.
Nanomaterial induced endothelial cell leakiness (NanoEL) is caused because nanomaterials enter the interstitial space of the endothelial cells and disrupt the endothelial cell–cell interactions by interacting with vascular endothelial cadherin (VE-cad). Whereas the NanoEL effect could cause controllable leakiness in cancer therapy, the gaps created by the NanoEL effect can make the cancer cells cross the endothelial barrier and produce side effects induced by using nanomedicine. In this paper, a series of ultralow protein corona nanoparticle is reported that can penetrate the endothelial cell junction without obviously interacting with the VE-cad and phosphorylating the tyrosine 658 (Y658) and tyrosine 731 (Y731) residues on VE-cad, thus preventing the VE-cad from being activated by Src kinase, and this avoids inducing of the NanoEL effect and cancer cell migration, regardless of particle material, density and surface charge. These findings provide a new idea for the design of novel nanoparticles without side effects and can maximize their cancer-killing effect.
危险品化学品经营许可证（带存储）