SOUTH AFRICAN JOURNAL OF BOTANY
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2023
·
IF 3.1
Hui Li,Keren Agyekumwaa Addo,Yi-gang Yu,Xing-long Xiao
In Iranian, ethnopharmacological studies show that Cuminum cyminum is commonly used in pain relief. The most abundant component in C. cyminum seeds is cuminaldehyde (CUM). However, there are few articles that have evaluated the anti-biofilm activity against Staphylococcus aureus ( S. aureus ). The nobility of this study was to evaluate the anti-biofilm effect of CUM and to investigate its possible mechanism of action. Crystal violet staining assay showed that CUM decreased biofilm biomass. The morphological observation indicated that CUM restrained the adhesion of biofilm, and destroyed the integrated and cohesive structure of biofilm. MTT assay proved that the metabolic activity of adherent cells treated with CUM was reduced. In vitro assays exhibited that cell motility, cell surface hydrophobicity, and extracellular polymers production of S. aureus were also inhibited in the presence of CUM. Furthermore, quantitative real-time PCR analysis confirmed the ability of CUM to interfere with the expression of adhesion genes associated with biofilm formation such as agrA, icaA, sigB, ebpS, fnbA, fib and clfA . Molecular docking speculated that the interaction of CUM with SarA could inhibit SarA-DNA binding, and ultimately inhibit the formation of biofilm. The results of this trial provided scientific experimental data for the use of CUM as an anti-biofilm agent.
研究领域:
微生物学
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药理学
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分子生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2022
·
IF 5.911
Hui Li,Yan-yan Huang,Keren Agyekumwaa Addo,Ze-xuan Huang,Yi-gang Yu,Xing-long Xiao
The previous study indicated that cuminaldehyde (CUM) could be used as an antibacterial agent in sauced beef to reduce the propagation of Staphylococcus aureus ( S. aureus ). This research took sauced beef treated with 0.4 μL/mL CUM as the research object. Transcriptomic and proteomic methods were used to comprehensively analyze the changes in genes and proteins of S. aureus under CUM stress. A total of 258 differentially expressed genes (DEGs, 178 up-regulated and 80 down-regulated) and 384 differentially expressed proteins (DEPs, 61 up-regulated and 323 down-regulated) were found. It was observed that CUM destroyed the cell wall and cell membrane by inhibiting the synthesis of peptidoglycan and fatty acid. Low energy consumption strategies were formed by reducing glycolysis and ribosome de novo synthesis. The levels of genes and proteins associated with the glycine, serine , threonine , methionine , cysteine, and branched-chain amino acids were dramatically changed, which impaired protein synthesis and reduced bacterial viability . In addition, the up-regulated DEGs and DEFs involved in DNA replication , recombination and single-stranded DNA-binding contributed to DNA repair . Moreover, ATP-binding cassettes (ABC) transporters were also perturbed, such as the uptake of betaine and iron were inhibited. Thus, this study revealed the response mechanism of S. aureus under the stress of CUM, and provided a theoretical basis for the application of CUM in meat products.
研究领域:
微生物学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2022
·Vol. 189
·期 3
·1-9
·
IF 6.408
Gao Huanli,Xu Tingting,Zhou Jiahuan,Rojas Orlando J.,He Ming,Ji Xingxiang,Dai Hongqi
A system for the rapid and ultra-sensitive detection of Staphylococcus aureus ( S. aureus ), a prevalent foodborne pathogen is introduced. Limitations of typical electrochemical sensing, often subjected to interference from non-specific protein adsorption are addressed. A dual-aptamer-based sandwich immunobiosensor is shown for its benefits regarding specificity and anti-fouling capacity, endowed by a sulfonated polyaniline layer combined with signal amplification via highly conductive gold nanoparticles. EIS spectra (Nyquist plots) were recorded at pH 7.4 PBS containing 5 mM Fe(CN) 6 3− /Fe(CN) 6 4− , in order to verify the possibility of the electrochemical sensing for detection of S. aureus. Results demonstrated that the constructed immunobiosensor presents an extended detection range (1 × 10 1 to 1 × 10 5 CFU/mL) and detection limit as low as 2 CFU/mL. The resistance values of the immunobiosensor developed maintain at a stable value during 2 weeks. Besides, the specificity of the system is highlighted by testing raw milk, and the results of which demonstrate the excellent prospects of the system for monitoring foodborne pathogens. Graphical abstract
研究领域:
食品科学
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生物传感器技术
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
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IF 8.1
Danliangmin Song,Ai Jia,Xuehe Qi,Kai Dong,Shiyu Liu,Chaoxin Man,Xinyan Yang,Yujun Jiang
Bacterial biofilm is a protective matrix composed of metabolites secreted by bacteria that envelop bacteria. By forming a biofilm, bacteria can considerably improve their environmental tolerance. In food-related processing environment, different types of microorganisms are often present in biofilms. The main contaminating strain in the powdered infant formula (PIF) processing environment, Cronobacter sakazakii and Staphylococcus aureus continues to pollute the PIF processing environment after biofilm production. This study selected Cronobacter sakazakii with a weak biofilm-forming ability as one of the test organisms. The coexistence of Cronobacter sakazakii and Staphylococcus aureus on the surface of production equipment was simulated to analyze the interaction. Biofilm formation in the co-culture group was significantly higher than the others. In-depth study of the effect of Staphylococcus aureus on the biofilm formation genes of Cronobacter sakazakii . Results show two bacteria can coexist on the surface of a metal device, forming a more compact hybrid biofilm structure. Under co-culture conditions, S. aureus increased bcsA and fliD expression in Cronobacter sakazakii , whereas decreased bcsC expression. Signaling molecules produced by Staphylococcus aureus (Autoinducer 2) significantly promoted the biofilm formation of Cronobacter sakazakii at the concentration of 0–500 ng/mL (0.099–0.177) and up-regulated the expression of bcsA , filD and flhD genes.
研究领域:
微生物学
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食品科学
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DOI
PubMed
高影响因子
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
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2021
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IF 3.927
Dongpo Xu,Yan Chen,Tianyi Qiu,Shuyan Qi,Li Zhang,Ming Yin,Kangjie Ge,Xiuxia Wei,Xinxin Tian,Pinggao Wang,Mingyuan Li,Jingxuan Qiu
In this work, the Staphylococcus aureus ( S. aureus ) was firstly used as a template coupled with PVP in electrospinning experiments, Sn 2+ bonded with S. aureus and PVP to form nanofiber through electrospinning. Finally, we got the hierarchical mesoporous SnO 2 nanotube after calcination of the nanofiber . The hierarchical mesoporous SnO 2 nanotube as a gas sensor toward triethylamine (TEA) shows high sensitivity, better cycling stability and selectivity. The response and recovery time of the hierarchical mesoporous SnO 2 nanotube to 10 ppm TEA are about 19 s and 10 s.
研究领域:
材料科学
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生物学
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传感器技术
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-11
·
IF 5.3
Zhang Xiaoyu,Sun Ruimeng,Zheng Haoran,Qi Yanfei
A spherical nucleic acid (SNA, AuNPs-aptamer) into CRISPR/Cas12a system combined with poly T-template copper nanoparticles as fluorescence reporter was fabricated to establish an amplification-free sensitive method for Staphylococcus aureus ( S. aureus ) detection. This method, named PTCas12a, utilizes the concept that the bifunction of SNA recognizes the S. aureus and triggers the Cas12a cleavage activity. Then, the Cas12a enzyme cleaves the Poly T40 to generate a signal change in Poly T-Cu fluorescence, indicating the presence or absence of the target bacteria. The PTCas12a platform demonstrated a detection limit as low as 3.0 CFU/mL (3 N/S) in a wide response range of 1.0 × 10 1 –1.0 × 10 6 CFU/mL for S. aureus detection, which holds significant potential in ensuring food safety and preventing the spread of diseases. Graphical abstract
研究领域:
生物技术
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医学诊断
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食品安全
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DOI
PubMed
高影响因子
RSC Advances
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2019
·Vol. 9
·期 30
·17002-17015
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IF 3.049
Kishore Chand,M. Ishaque Abro,Umair Aftab,Ahmer Hussain Shah,Muhammad Nazim Lakhan,Dianxue Cao,Ghazanfar Mehdi,Abdalla Mohamed Ali Mohamed
Recently, it has been shown that silver nanoparticles (AgNPs) exhibit great potential for different applications, including food storage, cosmetic products, electronic components, biosensor materials, cryogenics, dental materials and especially for drug-delivery activities. In this study, we synthesized AgNPs with neem extract (NE) alone and mixed plant extracts of neem, onion and tomato (NOT) as a combined reducing and stabilizing agent by a green synthesis method at different pHs. The synthesized products were characterized by ultraviolet-visible spectroscopy (UV-vis), X-ray diffraction (XRD), dynamic light scattering (DLS), atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The antibacterial effects of the synthesized products were studied by the Kirby disk diffusion method. It was confirmed that the AgNPs work effectively as a drug material against Gram-positive bacteria Staphylococcus aureus in nutrient agar. In addition, it was seen that the reducing and stabilizing agent NOT could work effectively with six medicines with a different nature at the maximum addition of 15 μg. However, the synthesized product with NE alone only worked for four of the medicines. Therefore, it was evident that the AgNPs synthesized with NOT extract were more susceptible to the Gram-positive bacteria Staphylococcus aureus. We believe that this new route for synthesizing AgNPs with NOT extract could be more beneficial in comparison to NE alone for improved antibacterial properties in drug-delivery applications.
研究领域:
微生物学
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纳米生物学
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药物化学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Yahui He,Jian Zhou,Jinbo Zhang,Yihao Guo,Zhangbin Ji,Hui Chen,Yongqing Fu
Love-mode surface acoustic wave (SAW) sensors show great promise for biodetection applications owing to their low cost, digital output, and wireless passive capability, but their performance is often restricted by the availability of suitable sensitive membrane layers. Herein, a composite layer of electrospun fibers made from cellulose acetate and polyethylenimine, coated with gold nanoparticles, is proposed as a porous and sensitive membrane coated onto a love-mode SAW biosensor for monitoring gene sequences of Staphylococcus aureus. The results showed that the developed sensor exhibited an impressive sensitivity of 122.56 Hz/(nmol/L) for detecting gene sequences of S. aureus, surpassing the sensitivity of conventional SAW sensors employing a bare Au film as the sensitive layer by 5-fold. The analysis revealed a remarkably linear detection (R2 of 0.97827) of S. aureus gene sequences within the range of 0 to 100 nmol/L. The limit of detection was impressively low at 0.9116 nmol/L. The good stability and specificity of the biosensor in liquid environments were demonstrated for clinical diagnostics.
研究领域:
生物医学工程
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOLOGICAL & PHARMACEUTICAL BULLETIN
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2015
·Vol. 38
·期 7
·1041-1048
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IF 1.828
Huixian Liu,Sui Xiao,Depeng Zhang,Shuhua Mu,Lifang Zhang,Xiaoyang Wang,Feiqun Xue
In this study we describe the design, synthesis, and antibacterial activity of novel pleuromutilin analogs. A series of new compounds containing piperazine and alkylamino or arylamino groups was synthesized. The new compounds were characterized via 1H-NMR, 13C-NMR, Fourier transform (FT)-IR and MS, and were further evaluated for their in vitro activity against seven Gram-positive, and one Gram-negative, pathogens. Antibacterial data revealed that all compounds exhibited moderate to good antibacterial activities against sensitive Gram-positive pathogens. Specifically, 9d displayed the best activity: its activity to Staphylococcus aureus (ATCC25923) is 0.125 µg/mL, which is equal to the control compound tiamulin. The antibacterial activities of 9d to Streptococcus suis (minimum inhibitory concentration (MIC) of 2 µg/mL), Streptococcus agalactiae (MIC of 0.5 µg/mL), and Streptococcus dysgalactiae (MIC of 0.5 µg/mL) were also excellent compared with the control drug erythromycin (MIC of >128 µg/mL). The binding modes of these compounds with active sites were calculated using the programs of Molecular Operating Environment (MOE) and Pymol.
研究领域:
药理学
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有机合成化学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 11
·6335-6344
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IF 4.682
Liu Lingling,Zhou Shu,Luo Liping,Zheng Jingjing,Ji Lijun
Ag@C fibres with porous silver structure were synthesized with a surface-hydrolyzed polyacrylonitrile (PAN) fibre as a template and by a hydrazine hydrate reduction and carbonization process. The composition and structure of the Ag@C fibres were characterized with a scanning electron microscope, an X-ray diffractometer, a Fourier transform infrared spectrometer, an X-ray photoelectron spectrometer, a Raman spectrometer, and a thermogravimetric analyser. The porous structure of the silver shell combined with carbon was conducive to the release of silver ions and suppressed the loss of silver nanoparticles from the surface of the carbon fibres. The Ag@C fibres showed excellent antibacterial performance. When the dosage of Ag@C fibres prepared with a silver nitrate solution of 0.5 mol/L was 1 mg, 2 mg, 4 mg and 8 mg, the bacterial inactivation rate to Staphylococcus aureus was 97.98%, 99.89%, 99.99%, and 99.99% in 12 h, respectively. The loss of viability only decreased to 80% when 1 mg of Ag@C fibres were used for antimicrobial activity test after five times of recycling. The Ag@C fibres have a broad application prospect in producing fabric products of antibacterial fields. Graphical abstract
研究领域:
材料科学
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微生物学
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 52
·期 5
·2473-2483
·
IF 2.302
Zhu Lixia,Dai Jinyue,Chen Lai,Chen Jing,Na Haining,Zhu Jin
By covalently immobilizing imidazolium ion onto molecular chain, functional polyurethane (PU) is fabricated and thus an effective way is initiated to prepare electrospun membranes with antibacterial activity. In the experiment, PUs containing imidazolium ion side group (Bmim-PUs) are synthesized through a two-step polymerization process. It includes prepolymerization of isophorone diisocyanate (IPDI) with polyester glycol and chain extension polymerization using imidazolium-based ionic diol (Bmim-OH). Then, the obtained Bmim-PUs are electrospun into fibrous membranes with a diameter of ~640 nm. After a careful assessment, antibacterial activities of electrospun membranes against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli are clearly exhibited. The antibacterial efficiency of Bmim-PUs on both bacteria species improves by 60% in comparison with PU without imidazolium ion. This research suggests a simple but effective methodology to design and fabricate ultrafine fibrous membrane with significant antibacterial activity. Moreover, the obtained fibrous membranes have widely potential applications in protective textiles, filtration, and biomedical engineering.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2014
·Vol. 16
·期 7
·1-12
·
IF 2.278
Zhao Lianhong,Yan Xiufang,Jie Zhiqiang,Yang Hong,Yang Shiping,Liang Jie
Regenerable antimicrobial N -halamine/silica hybrid nanoparticles (NPs) containing chlorinated 5,5-dimethylhydantoinyl (Cl-DMH) groups, Cl-DMH/SiO 2 hybrid NPs, have been prepared by a co-condensation reaction between N -(3-triethoxysilylpropyl)-5,5-dimethylhydantoin (TS-DMH) and tetraethoxysilane (TEOS) and then a chlorination reaction in NaClO solution. The as-synthesized Cl-DMH/SiO 2 NPs were characterized by transmission electron microscopy, Scanning electron microscopy, X-ray photoelectron spectra, Specific surface area, Differential scanning calorimetry, and Fourier transform infrared. Experimental results showed that the size of the as-synthesized Cl-DMH/SiO 2 NPs could be well adjusted by changing the mass ratio of TS-DMH/TEOS and the volume ratio of 28 % NH 4 OH/H 2 O. Antimicrobial tests showed that the as-prepared Cl-DMH/SiO 2 hybrid NPs had excellent antimicrobial activities against both Escherichia coli and Staphylococcus aureus . The minimum inhibitory concentration and minimum bactericidal concentration values of the as-prepared Cl-DMH/SiO 2 hybrid NPs are 15 and 20 μg/mL for S. aureus , 25 and 30 μg/mL for E. coli , respectively. Paper disk diffusion assay showed that smaller-sized Cl-DMH/SiO 2 hybrid NPs have bigger inhibition zone diameters, indicating stronger antimicrobial efficacies. Also, the storage stability and regenerability of Cl-DMH/SiO 2 hybrid NPs were investigated.
研究领域:
材料科学
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微生物学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）