JOURNAL OF INVERTEBRATE PATHOLOGY
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2024
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IF 3.6
Peng-hua Bai,Jin-Ping Yu,Rui-rui Hu,Qian-wen Fu,Hai-chao Wu,Xing-yue Li,Guo-hao Zu,Bao-sheng Liu,Yu Zhang
Entomopathogenic nematodes (EPNs) use the chemical cues emitted by insects and insect-damaged plants to locate their hosts. Steinernema carpocapsae , a species of EPN, is an established biocontrol agent used against insect pests. Despite its promising potential, the molecular mechanisms underlying its ability to detect plant volatiles remain poorly understood. In this study, we investigated the response of S. carpocapsae infective juveniles (IJs) to 8 different plant volatiles. Among these, carvone was found to be the most attractive volatile compound. To understand the molecular basis of the response of IJs to carvone, we used RNA-Seq technology to identify gene expression changes in response to carvone treatment. Transcriptome analysis revealed 721 differentially expressed genes (DEGs) between carvone-treated and control groups, with 403 genes being significantly upregulated and 318 genes downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the responsive DEGs to carvone attraction were mainly involved in locomotion, localization, behavior, response to stimulus, and olfactory transduction. We also identified four upregulated genes of chemoreceptor and response to stimulus that were involved in the response of IJs to carvone attraction. Our results provide insights into the potential transcriptional mechanisms underlying the response of S. carpocapsae to carvone, which can be utilized to develop environmentally friendly strategies for attracting EPNs.
研究领域:
昆虫学
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分子生物学
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植物科学
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生物防治
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2020
·Vol. 20
·期 3
·1470-1477
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IF 1.134
Makirita Winisia E.,Zhang Fengqin,Mbega Ernest R.,He Nongyue,Li Xiaolong,Chacha Musa,Liu Tonghua
Entomopathogenic nematodes (EPNs) have been cited as a safe and effective method for pest management. Their virulence against lepidopterans and other foliar pest has been demonstrated in the laboratory, but achieving field efficacy is challenged with environmental conditions such as
desiccation and ultraviolet radiations. Nanoparticles (NPs) as UV-blocking agents have been reported to provide an alternative approach to enhance the EPNs efficacy. We screened the effect of ZnO, TiO2 and Fe3O4 NPs on survival and pathogenicity of EPNs, and
determined whether the nanoparticles could provide protection to the EPNs at low concentrations when applied in direct sunlight. All nanoparticles had less influence on the survival of infective juveniles and did not deprive their pathogenic properties following prolonged exposure. The survival
rate of nematodes decreased with increased concentrations, with no measurable difference between NPs. Moreover, the survival rate of nematodes exposed to UV radiation in different formulation decreased significantly with the increase in exposure time (p < 0.001). On the other side
when EPNs in nanoparticles formulations were exposed to sunlight, their efficacy significantly improved by protecting nematodes from ultraviolet radiation when compared to the water-based formulation. Pathogenic efficacy after exposure to sunlight was in the order of ZnO > TiO2
> Fe3O4 > H2O, indicating the compatibility of nematodes and NPs, and the benefit of different NPs in EPNs formulations. General nanoparticles are novel ingredients that provide suitable protection of EPNs for management of foliar pests. However, the
EPNs nanoparticles formulation under a specific agricultural system and climatic condition need to be established.
研究领域:
生物学
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农业科学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2020
·Vol. 20
·期 3
·1434-1439
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IF 1.134
Makirita Winisia E.,Yong Liu,He Nongyue,Mbega Ernest R.,Chacha Musa,Li Xiaolong,Zhang Fengqin
Nanoparticles (NPs) are technological engineered materials with unique physical and chemical properties, and dimension of less than 100 nm. Nanotechnology has developed at a rapid pace, resulting into tremendous wide application that has resulted into concerns and ecotoxicological consequences.
The antimicrobial potentials of the nanoparticles have been extensively studied, however, little has been done on the allied health and environmental toxicity assessments. Thus, the current work evaluated the toxicity effects of the ZnO, TiO2 and Fe3O4 NPs
on the survival of the entomopathogenic nematodes (Steinernema carpocapsae), as well as their growth inhibition effects on the nematode symbiotic bacteria (Xenorhabdus nematophila). The metal oxides NPs were characterized by scanning electron microscope and transmission electron
microscope. Their toxicity effects were evaluated at various concentrations with the consideration of the media on the toxicity influence. All metal oxides had less influence on the survival of the entomopathogenic nematode and growth of the nematode symbiotic bacterial partner in a concentration
dependant manner NPs. The observed toxicity was in the order of Fe3O4 < TiO2 < ZnO NPs respectively, with no significant difference between the NPs. The less toxic effect of the NPs noted may be associated with the ability of entomopathogenic nematodes
and their bacterial partner to tolerate toxicants. Nonetheless, other toxicity parameter of NPs on the beneficial nematodes needs to be evaluated for consideration of the compatibility potential of the nematodes and NPs for pest management.
研究领域:
环境科学
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生物学
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毒理学
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DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2025
·
IF 2.4
Tian Jingyin,Zhao Guoyu,Zhou Xia,Hu Feng,Shao Kangmeng,Dassanayaka Sajith Madhusanka,Jin Linhong
The successful commercialization of entomopathogenic nematodes (EPNs) as a biopesticide product depends on industrial mass production of viable infective juveniles (IJs) at low cost. This study focused on enhancing the yield of the Steinernema carpocapsae All (Weiser) and reducing its production costs. An optimal basal medium was screened among several basal media, and several optimized components were included in the selected optimal basal medium. The optimal medium formulation was obtained by evaluating the yield, virulence, and survival rates of S. carpocapsae All at different times. The results showed that when isopropylstilbene was added to the basal medium together with insulin, the yield increased from 13.14 × 104 IJs/ml with the basal medium to 19.63 × 104 IJs/ml. At 30 d of low temperature storage, the survival rate increased from 31.42% (basal medium) to 56.83% (optimal medium), demonstrating a prolonged the shelf life. The virulence of S. carpocapsae All was measured before and after 30 d of storage on sponges. The virulence against Tenebrio molitor (Linnaeus) (Coleoptera: Tenebrionidae) and Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) was 90 and 86.67% (before) and 93.33 and 94.44% (after), respectively, when insects were treated at 48 h, long-term cryogenic storage has no effect on the virulence. The yield with the individual optimization components added to the optimal basal medium was lower than when the components were combined, indicating that there may be a synergy between the 2 components. The results would provide practical technology for low-cost commercial production of S. carpocapsae All and promote field applications.
研究领域:
农业昆虫学
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生物防治
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线虫学
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微生物发酵
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应用昆虫学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）