MICROCHIMICA ACTA
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2019
·Vol. 186
·期 12
·1-9
·
IF 5.479
Shi Jianrong,Li Geyuan,Cui Yanru,Zhang Yan,Liu Donghao,Shi Yi,He Hua
β-Cyclodextrin-functionalized carbon nitride nanosheets were modified with a molecularly imprinted polymer to obtain a fluorescent probe of type MIP@β-CD/CNNS which is shown to enable fluorometric determination of sterigmatocystin (STG). The material was characterized by transmission electron microscopy, infrared spectra, powder X-ray diffraction, X-ray photoelectron spectroscopy, and by absorption and emission spectra. The modified CNNSs have a good fluorescence quantum yield (13%), high sorption capacity for STG (86 mg·g −1 ), fast adsorption rate (25 min), and superior adsorption selectivity (with an imprint factor 2.56). When used as an optical probe for STG, the CNNSs act as the chromophore, while β-CD and MIP act as the recognition groups. The blue fluorescence of MIP@β-CD/CNNS (with excitation/emission maxima at 368/432 nm) is quenched by STG. Fluorescence drops linearly in the 0.15 to 3.1 μM STG concentration range. The lower detection limit is 74 nM. The method was successfully applied to the determination of STG in spiked wheat extract. Conceivably, this detection scheme based on a combination of β-CD inclusion and molecular imprinting may be extended to the detection of various other organic compounds.
Schematic representation of the preparation of surface-imprinted β-cyclodextrin-functionalized carbon nitride nanosheets. These are used, along with a molecularly imprinted polymer, for fluorometric determination of sterigmatocystin.
研究领域:
分析化学
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食品安全
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材料科学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
·
IF 4.8
Jun Huang,Yaoyao Su,Xiaoman Chen,Gang Yang,Zhirong Wang,Kewei Chen,Muying Du,Zsolt Zalán,Ferenc Hegyi,Jianquan Kan
Sterigmatocystin (STC), a mycotoxin that pollutes wheat and other grains and is related to health risks, has attracted increasing attention. In this study, we have explored the toxicity conditions of Aspergillus versicolor in wheat and identified a potential biological control method. By constructing a water activity (Aw)-water content curve, the effects of temperature, Aw, and culture time on toxin production by A . versicolor in wheat were determined. The Aw was found to be 0.96, and the concentration of STC in wheat reached the maximum (105.7 mg/kg) after 10 d of culture at 28 °C. Biocontrol bacteria were screened using plate confrontation, agar diffusion, and co-culture in wheat. The results showed that Bacillus subtilis HJ4 has significant antifungal activity against A. versicolor in vitro and in vivo . In vivo experiments showed that B. subtilis HJ4 significantly reduced the toxicity of A. versicolor to wheat (51%) on day-7, but the control effect was weakened (10%) on day-20. Finally, research on the antifungal mechanism of B. subtilis HJ4 revealed that its action primarily relies on competition for space and nutrients, destroying the cellular structure of the fungus and secreting substances with antifungal activity, such as proteins, cyclic lipopeptides, and oligosaccharides, aiding in control against A. versicolor . In summary, this study has emphasized the influence of environmental factors on the growth and toxin production of A. versicolor in wheat and evaluated the potential of B. subtilis HJ4 in reducing the pollution caused by A. versicolor and STC in wheat.
研究领域:
食品科学
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微生物学
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毒理学
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产品关联
DOI
高影响因子
Journal of Ovarian Research
·
2024
·Vol. 17
·期 1
·1-16
·
IF 3.8
Wang Shiwei,Wu Xuan,Yang Juan,Peng Yuwan,Miao Fulu,Li Min,Zeng Juan
Background Sterigmatocystin (STE) is a mycotoxin widely found in contaminated food and foodstuffs, and excessive long-term exposure to STE is associated with several health issues, including infertility. However, there is little information available regarding the effects of STE toxin on the female reproductive system, particularly concerning oocyte maturation.Methods In the present study, we investigated the toxic effects of STE on mouse oocyte maturation. We also used Western blot, immunofluorescence, and image quantification analyses to assess the impact of STE exposure on the oocyte maturation progression, mitochondrial distribution, oxidative stress, DNA damages, oocyte ferroptosis and asymmetric division defects.Results Our results revealed that STE exposure disrupted mouse oocyte maturation progression. When we examined the cellular changes following 100 µM STE treatment, we found that STE adversely affected polar body extrusion and induced asymmetric division defects in oocytes. RNA-sequencing data showed that STE exposure affects the expression of several pathway-correlated genes during oocyte meiosis in mice, suggesting its toxicity to oocytes. Based on the RNA-seq data, we showed that STE exposure induced oxidative stress and caused DNA damage in oocytes. Besides, ferroptosis and α-tubulin acetylation were also found in STE-exposed oocytes. Moreover, we determined that STE exposure resulted in reduced RAF1 protein expression in mouse oocytes, and inhibition of RAF1 activity also causes defects in asymmetric division of mouse oocytes.Conclusion sCollectively, our research provides novel insights into the molecular mechanisms whereby STE contributes to abnormal meiosis.
研究领域:
生殖毒理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2017
·Vol. 9
·期 7
·299
·
IF 3.364
Jing-Min Liu,Feng-Zhen Cao,Guo-Zhen Fang,Shuo Wang
Originated from the bottom-up synthetic strategy, molecularly imprinted polymers (MIPs) possess the inherent ability of selective and specific recognition and binding of the target analytes, with their structural cavities that can match the target molecules in respect to size, shape, and functional groups. Herein, based on the high selectivity of MIPs and the fluorescence properties of the β-NaYF4:Yb3+, Er3+upconversion nanoparticles, MIPs with both specificity and fluorescent signals are fabricated to recognize trace sterigmatocystin (ST) with high selectivity and sensitivity. The structure analogue of ST, 1,8-dihydroxyanthraquinone (DT), was employed as the template molecule, acrylamide as the functional monomer, 3-methacryloyloxypropyltrimethoxysilane as the crosslinking agent, and a new molecular imprinting technique of non-aqueous sol-gel method is used to synthesize a molecularly imprinted material with high selectivity to ST. Under optimal conditions, the fluorescence enhancement of fluorescent MIPs increased as the concentration of ST increased. In the range of 0.05–1.0 mg L−1, fluorescence enhancement and the concentration showed a good linear relationship with a detection limit of 0.013 mg L−1. Real sample analysis achieved the recoveries of 83.8–88.8% (RSD 5.1%) for rice, 82.1–87.5% (RSD 4.6%) for maize, and 80.6–89.2% (RSD 3.0%) for soybeans, respectively, revealing the feasibility of the developed method.Keywords:sterigmatocystin (ST);upconversion nanophosphors;molecularly imprinted polymers;fluorescence detection
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2023
·
IF 6.1
Yaqi Zhang,Yong Man,Jingna Li,Yaming Sun,Xiuming Jiang,Lijun He,Shusheng Zhang
The aflatoxin B 1 (AFB 1 ) early warning technique based on precursors is an effective strategy for the prevention of AFB 1 contamination risk. The determination of precursors is imperative to ensure the efficiency of the early warning technique. Herein, a controllable magnetic adsorbent Fe 3 O 4 /ZIFs was first introduced for the effective extraction and determination of averantin (AVN) and sterigmatocystin (ST) precursors in cereal by combining magnetic solid-phase extraction (MSPE) and high-performance liquid chromatography (HPLC). Benefiting from the abundant adsorption sites and multifunctional groups matching the analytes , Fe 3 O 4 /ZIFs effectively and simultaneously extracted AVN and ST with great differences in polarity and structure via multiple interactions. AVN was extracted by Fe 3 O 4 /ZIFs mainly through π–π and hydrophobic interactions, while ST was extracted predominantly by electrostatic interactions and surface complexation. The limits of detection were 0.08 μg kg −1 (AVN) and 0.36 μg kg −1 (ST). The developed method exhibited satisfactory spiked recoveries (79.1%–105.4%) in the determination of AVN and ST in rice. This work provides a novel analytical strategy for further studying AFB 1 early warning technique and the formation and transformation of aflatoxins .
研究领域:
分析化学
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食品安全
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毒理学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
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2020
·
IF 4.236
Li Yuanjing,Yan Pengfei,Lu Xiaojie,Qiu Yanling,Liang Shang,Liu Gang,Li Shuangfei,Mou Lin,Xie Ning
The sucrose non-fermenting 1/AMP-activated protein kinase (SNF1/AMPK) is a central regulator of carbon metabolism and energy production in the eukaryotes. In this study, the functions of the Podospora anserina SNF1 (PaSNF1) ortholog were investigated. The ΔPaSNF1 mutant displays a delayed development of mycelium and fruiting bodies and fails to form ascospores. The expression of the PaSNF1 gene in the strain providing female organs in a cross is sufficient to ensure fertility, indicating a maternal effect. Results of environmental stress showed that ΔPaSNF1 was hypersensitive to stress, such as osmotic pressure and heat shock, and resistant to fluconazole. Interestingly, the knockout of PaSNF1 significantly promoted sterigmatocystin (ST) synthesis but suppressed cellulase [filter paperase (FPA), endoglucanase (EG), and β-glucosidase (BG)] activity. Further, transcriptome analysis indicated that PaSNF1 made positive regulatory effects on the expression of genes encoding cellulolytic enzymes. These results suggested that PaSNF1 may function in balancing the operation of primary and secondary metabolism. This study suggested that SNF1 was a key regulator concerting vegetative growth, sexual development, and stress tolerance. Our study provided the first genetic evidence that SNF1 was involved in the ST biosynthesis and that it may also be a major actor of lignocellulose degradation in P. anserina.
研究领域:
微生物学
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分子生物学
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真菌遗传学
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酶学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2024
·
IF 5
Jun Huang,Gang Yang,Kewei Chen,Muying Du,Zsolt Zalán,Ferenc Hegyi,Jianquan Kan
Sterigmatocystin (STC) is an emerging mycotoxin that poses a significant threat to the food security of cereal crops. To mitigate STC contamination in maize, this study employed selected lactic acid bacteria as biocontrol agents against Aspergillus versicolor , evaluating their biocontrol potential and analyzing the underlying mechanisms. Lactiplantibacillus plantarum HJ10, isolated from pickle, exhibited substantial in vitro antifungal activity and passed safety assessments, including antibiotic resistance and hemolysis tests. In vivo experiments demonstrated that L. plantarum HJ10 significantly reduced the contents of A. versicolor and STC in maize (both >84 %). The impact of heat, enzymes, alkali, and other treatments on the antifungal activity of cell-free supernatant (CFS) was investigated. Integrated ultra-high-performance liquid chromatography (UPLC) and gas chromatography–mass spectrometry (GC–MS) analysis revealed that lactic acid, acetic acid, and formic acid are the key substances responsible for the in vitro antifungal activity of L . plantarum HJ10. These metabolites induced mold apoptosis by disrupting cell wall structure, increasing cell membrane fluidity, reducing enzyme activities, and disrupting energy metabolism. However, in vivo antagonism by L . plantarum HJ10 primarily occurs through organic acid production and competition for growth space and nutrients. This study highlights the potential of L . plantarum HJ10 in reducing A. versicolor and STC contamination in maize.
研究领域:
微生物学
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食品科学
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毒理学
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农业科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Ruya Wang,Pu Wu,Yanru Cui,Meriem Fizir,Jianrong Shi,Hua He
Two thermo-responsive molecularly imprinted polymers (MHNTs@MIP and MCNTs@MIP) for the selective extraction of sterigmatocystin have been prepared on the surface of the magnetic halloysite nanotubes (MHNTs) and magnetic carbon nanotubes (MCNTs), respectively. 1, 8-dihydroxyanthraquinone, n-isopropyl acrylamide, methacrylic acid, ethylene dimethacrylate and dimethyl sulfoxide were used as the dummy template, thermo-sensitive functional monomer, co-monomer, cross-linker and porogen, respectively. The magnetic properties, adsorption properties as well as the temperature responsive behaviors of MHNTs@MIP and MCNTs@MIP were systematically studied and compared for the first time. Enough saturation magnetizations of MHNTs@MIP (9.42 emu/g) and MCNTs@MIP (10.54 emu/g) were obtained. MHNTs@MIP and MCNTs@MIP also showed controllable adsorption and release behaviors to sterigmatocystin in response to the temperature change (35 °C and 20 °C). Compared with MCNTs@MIP, MHNTs@MIP had higher adsorption affinity (K L = 0.120 L/mg), higher adsorption kinetic (K 2 = 0.0100 g/(mg•min)) and higher imprinting factor (5.22) to sterigmatocystin. These results indicated that MHNTs@MIP was favorable adsorbent for the selective separation of sterigmatocystin. Furthermore, the elution conditions of MHNTs@MIP were optimized by response surface methodology. Under the optimal conditions, MHNTs@MIP coupled with high performance liquid chromatography were successfully applied to the selective recognition, purification, enrichment and detection of sterigmatocystin in wheat samples. The recoveries were calculated from 88.62% to 102.9% with RSDs less than 3.5 % and limit of detection of 1.1 μg/kg. This work provided a suitable carrier for the preparation of imprinted polymers and a practical approach for highly selective recognition and determination of analytes in real samples.
研究领域:
食品科学
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分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Qiuzheng Du,Yan Zhang,Lili Yu,Hua He
A novel magnetic molecularly imprinted polymers (MMIPs) with a new functional monomer Triallyl isocyanurate was synthesized successfully to enrich and detect sterigmatocystin (STG) in wheat samples. The differential UV–vis spectra and the reverse prediction method were selected to achieve the optimal synthesis conditions of the MMIPs, which were characterized well. The adsorption experiment showed that MMIPs have high selectivity and sensitivity. A magnetic solid phase extraction combined with high performance liquid chromatography (MSPE-HPLC) method based on the MMIPs was successfully established with the optimal extraction condition. The linear range and RSD were 1.8–25 ng·g −1 and 2.6–4.1%, respectively. The recovery of this method was 87.6–96.9% and the limit of detection (LOD) was 0.63 ng·g −1 . The excellent sensitivity and selectivity of this method were confirmed by experiment of the extraction and detection of STG in wheat extracts. This work extends the use of molecular imprinting in mycotoxins applications.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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